SEPARATION SCIENCE PLUS最新文献_第10页

Comparison study on extraction techniques used to assess S‐phenylmercapturic acid as low benzene exposure biomarker based on gas chromatography coupled with mass spectrometry 基于气相色谱-质谱联用的S-苯巯基尿酸低苯暴露生物标志物提取技术的比较研究

IF 1.1

SEPARATION SCIENCE PLUS Pub Date : 2023-08-13 DOI: 10.1002/sscp.202300031

V. G. Milagres, Maria José Nunes de Paiva, M. M. d'Auriol‐Souza, A. L. Larentis, I. C. Costa-Amaral, L. C. André

{"title":"Comparison study on extraction techniques used to assess S‐phenylmercapturic acid as low benzene exposure biomarker based on gas chromatography coupled with mass spectrometry","authors":"V. G. Milagres, Maria José Nunes de Paiva, M. M. d'Auriol‐Souza, A. L. Larentis, I. C. Costa-Amaral, L. C. André","doi":"10.1002/sscp.202300031","DOIUrl":"https://doi.org/10.1002/sscp.202300031","url":null,"abstract":"The risk assessment of benzene exposure can be performed based on the analysis of biomarkers in biological monitoring processes. S‐phenylmercapturic acid is a specific benzene biomarker, and its analysis challenges the sensitivity of several analytical methods. The present study aims to develop and standardize an analytic procedure to determine this biomarker through gas chromatography–mass spectrometry, based on different extraction methods, to increase the sensitivity and specificity of the studied analytical method. Liquid–liquid extraction, solid‐phase extraction, liquid‐phase microextraction, and low‐temperature partitioning extraction were tested. The last one was the selected extraction method, which was validated to ensure their quality and reliability. The method above has shown linear correlation to benzene concentrations ranging from 5 to 60 μg L−1 and presented detection and quantification limits of 0.95 and 3.18 μg L−1, respectively. Intra and inter‐assay accuracy recorded variation coefficients equal 3.63% and 8.67%, respectively. The mean accuracy value was 98.72%. This procedure presented linearity, accuracy, and detection and quantification limits within the range of interest adopted to assess occupational and environmental exposure to benzene based on biomonitoring processes.","PeriodicalId":21639,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45488356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In‐tube solid‐phase microextraction (sulfopropyl methacrylate‐co‐ethylene glycol dimethacrylate monolithic capillary) coupled to LC–MS/MS to determine beta amyloid peptides in Alzheimer's cerebrospinal fluid samples 管内固相微萃取（甲基丙烯酸磺丙酯-乙二醇二甲基丙烯酸酯整体毛细管）与LC–MS/MS联用测定阿尔茨海默氏症脑脊液样本中的β-淀粉样肽

IF 1.1

SEPARATION SCIENCE PLUS Pub Date : 2023-08-07 DOI: 10.1002/sscp.202300044

Caroline Fernandes Grecco, V. Tumas, J. Hallak, M. E. Queiroz

{"title":"In‐tube solid‐phase microextraction (sulfopropyl methacrylate‐co‐ethylene glycol dimethacrylate monolithic capillary) coupled to LC–MS/MS to determine beta amyloid peptides in Alzheimer's cerebrospinal fluid samples","authors":"Caroline Fernandes Grecco, V. Tumas, J. Hallak, M. E. Queiroz","doi":"10.1002/sscp.202300044","DOIUrl":"https://doi.org/10.1002/sscp.202300044","url":null,"abstract":"Alzheimer's disease (AD) is a neurodegenerative disorder characterized by an extracellular accumulation of amyloid beta peptides in the brain. The concentration of the amyloid beta peptides in cerebrospinal fluid (CSF) have been evaluated as potential biomarkers of AD for early diagnostic. In this work, an in‐tube solid‐phase microextraction (in‐tube SPME) coupled to UHPLC–MS/MS method was established to determine amyloid beta peptides in CSF samples from AD patients. A strong cation‐exchange (sulfopropyl methacrylate‐co‐ethylene glycol dimethacrylate) monolithic capillary was synthesized on the inner surface of a fused silica capillary and used as an extractive phase for in‐tube SPME. The morphology and chemical structure of the monolithic phase were characterized by scanning electron microscopy and Fourier transform infrared spectroscopy. The proposed method presented linear range from the lower limits of quantification 0.6 and 0.8 to 10 ng mL−1. Excluding the lower limits of quantification values, the analytical validation showed precision with the coefficient of variation values lower than 13.1%, and accuracy with relative standard deviation lower than 13.5%. The in‐tube SPME coupled to UHPLC–MS/MS method was successfully applied to determine amyloid beta peptides in CSF samples from AD patients.","PeriodicalId":21639,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43610037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A sensitive, precise, accurate, and economic planar chromatographic method developed and validated for quantification of Desidustat as per guideline 根据指南，开发并验证了一种灵敏、准确、经济的平面色谱法，用于Desidustat的定量

IF 1.1

SEPARATION SCIENCE PLUS Pub Date : 2023-08-04 DOI: 10.1002/sscp.202300069

Rutvik H Pandya, Hetaben M. Kachhiya, Dhavalsinh P. Solanki, Jinal N. Tandel, U. Chhalotiya, D. Shah

{"title":"A sensitive, precise, accurate, and economic planar chromatographic method developed and validated for quantification of Desidustat as per guideline","authors":"Rutvik H Pandya, Hetaben M. Kachhiya, Dhavalsinh P. Solanki, Jinal N. Tandel, U. Chhalotiya, D. Shah","doi":"10.1002/sscp.202300069","DOIUrl":"https://doi.org/10.1002/sscp.202300069","url":null,"abstract":"Anemia with chronic kidney disease treating newer hope is recently approved Desidustat. By following International Conference on Harmonization Quality guideline Q2 (R1), an economic high‐performance thin‐layer chromatography method has been developed for the estimation of Desidustat. Pre‐coated silica gel 60 F254 plates as stationary phase along with mobile phase toluene:methanol:glacial acetic acid (7.5:2.5:0.3 v/v/v), and 230 nm detection wavelength was selected as optimized. Developed method was found linear in quantity per band range of 100 ng/band to 600 ng/band for Desidustat with 0.9979 regression coefficient. Standard spiking method showed method accuracy with a mean percentage recovery of 99.21%–101.63%. Limit of detection and limit of quantitation were found to be 3.94 and 11.94 ng/band, respectively. The method was found to be linear, precise, accurate, robust, rugged, selective, sensitive, and specific for the quantification of Desidustat in bulk and tablet dosage form and successfully can be applied for the qualitative and quantitative determination of Desidustat in bulk and marketed tablet formulation.","PeriodicalId":21639,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41993460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Miniaturized liquid extraction cartridge with a functional porous polytetrafluoroethylene membrane for the determination of formaldehyde in gaseous samples 用于测定气体样品中甲醛的功能多孔聚四氟乙烯膜小型液体萃取盒

IF 1.1

SEPARATION SCIENCE PLUS Pub Date : 2023-08-01 DOI: 10.1002/sscp.202370076

引用次数: 0

Development, validation, and measurement uncertainty estimation of automated micro‐solid‐phase extraction coupled with liquid chromatography‐triple quadrupole mass spectrometry for the quantitative determination of anti‐tuberculosis drugs in milk samples 用于定量测定牛奶样品中抗结核药物的自动微固相萃取-液相色谱-三重四极杆质谱法的开发、验证和测量不确定度估计

IF 1.1

SEPARATION SCIENCE PLUS Pub Date : 2023-07-24 DOI: 10.1002/sscp.202300088

Ramya Thati, Seetha Bala Subrahanyam, A. Shaik, M. K. Mudiam

引用次数: 0

Green high‐performance thin layer chromatography‐densitometric approach for tablet formulation containing remogliflozin etabonate and vildagliptin 含瑞格列嗪依他酸盐和维达格利汀片剂的绿色高效薄层色谱-密度测定法

IF 1.1