SEPARATION SCIENCE PLUS最新文献_第6页

Application of sol‐gel universal sorbent coated fabric phase sorptive extraction membranes in combination with high‐performance liquid chromatography‐ultraviolet detection to monitor endocrine‐disrupting chemicals in milk and environmental water samples 应用溶胶-凝胶通用吸附剂涂层织物相吸附萃取膜结合高效液相色谱-紫外检测法监测牛奶和环境水样中干扰内分泌的化学物质

IF 1.1

SEPARATION SCIENCE PLUS Pub Date : 2023-12-08 DOI: 10.1002/sscp.202300101

Basit Olayanju, A. Kabir, N. Manousi, K. Furton

{"title":"Application of sol‐gel universal sorbent coated fabric phase sorptive extraction membranes in combination with high‐performance liquid chromatography‐ultraviolet detection to monitor endocrine‐disrupting chemicals in milk and environmental water samples","authors":"Basit Olayanju, A. Kabir, N. Manousi, K. Furton","doi":"10.1002/sscp.202300101","DOIUrl":"https://doi.org/10.1002/sscp.202300101","url":null,"abstract":"Here, we deployed sol‐gel universal sorbent‐coated fabric phase sorptive extraction membranes in tandem with high‐performance liquid chromatography equipped with an ultraviolet detector (HPLC‐UV) for the analysis of 10 endocrine‐disrupting chemicals (EDCs). Due to the varying polarities of the studied compounds indicated by their octanol/water partition coefficient ‘log Kow’ (1.47–5.07), the extraction membrane was designed with different functionalities that are capable of simultaneous interaction with compounds of diverse natures including polar, non‐polar, and ionic species via sol‐gel sorbent coating technology. An isocratic mode of HPLC‐UV at 55:45% acetonitrile:water (v/v) on a reverse phase C18 Zorbax column (5 μm, 150 mm, 4.6 mm) was used for the separation and quantitation. Calibration curves were found linear between 25 and 2000 ppb for all compounds except cortisone in the range of 25–1000 ppb which provided an R2 value above 0.9940 in all cases. The intra‐day reproducibility and inter‐day reproducibility were found in the range of 1.2–12.8 and 0.2–14.1 (expressed as percent relative standard deviation), respectively. The validated method was finally deployed for the analysis of environmental water and milk samples with estradiol showing the highest concentrations among the studied compounds.","PeriodicalId":21639,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138588630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemical analysis and high‐performance thin‐layer chromatography guided quantification of Boeravinone B from Boerhaavia diffusa root extracts: Assessment of in vitro safety analysis 植物化学分析和高效薄层色谱法指导量化白苧根提取物中的白苧酮 B：体外安全性分析评估

IF 1.1

SEPARATION SCIENCE PLUS Pub Date : 2023-12-07 DOI: 10.1002/sscp.202300140

T. P. S. Laasya, S. Thapliyal, K. Goel, Bhupinder Kumar, Ramarao Poduri, Gaurav Joshi

{"title":"Phytochemical analysis and high‐performance thin‐layer chromatography guided quantification of Boeravinone B from Boerhaavia diffusa root extracts: Assessment of in vitro safety analysis","authors":"T. P. S. Laasya, S. Thapliyal, K. Goel, Bhupinder Kumar, Ramarao Poduri, Gaurav Joshi","doi":"10.1002/sscp.202300140","DOIUrl":"https://doi.org/10.1002/sscp.202300140","url":null,"abstract":"An Indian traditional medicinal plant, Boerhaavia diffusa (Punarnava), is reported for its diuretic activity and helps in impaired kidney function. The present study indicates that alkaloids, carbohydrates, saponins, phytosterols, flavonoids, and glycosides are absent in the petroleum ether extract and present chiefly in the methanolic extract of roots. The phytosterols were found to be present in the ethyl acetate extract, while other phytochemical constituents, that is, alkaloids, carbohydrates, saponins, flavonoids, and glycosides, were absent. From high‐performance thin‐layer chromatography, the results indicated that out of all the three extracts and two marketed formulations, ethyl acetate root extract (LEA) has the highest amount of Boeravinone B content. Further, different root extracts of B. diffusa and two marketed formulations were evaluated on the human embryonic kidney cell line to understand pharmacological safety. From the results, it was observed that LEA and petroleum ether root extracts showed a higher percentage of cell survival and a better safety profile up to the dose of 1 mg/mL. The overall results indicated that further preclinical evaluation of B. diffusa is needed to understand the mechanism behind nephroprotective potential which can further expedite the drug discovery process of nephroprotective molecules.","PeriodicalId":21639,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138592448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New validated liquid chromatography‐tandem mass spectrometry method for the determination of Dacomitinib in human plasma and its application to a pharmacokinetic study 新型高效液相色谱-串联质谱法测定人血浆中的达科替尼并将其应用于药代动力学研究

IF 1.1

SEPARATION SCIENCE PLUS Pub Date : 2023-12-07 DOI: 10.1002/sscp.202300131

Tinku Gupta, NaliniKanta Sahoo, Maddela Rambabu, M. Praveen, Shrikant Charde, Madhusmita Sahu, Prasun Chakrabarti, Bui Thanh Hung, Martin Margala, B. Unhelkar, A. Narayanankutty

{"title":"New validated liquid chromatography‐tandem mass spectrometry method for the determination of Dacomitinib in human plasma and its application to a pharmacokinetic study","authors":"Tinku Gupta, NaliniKanta Sahoo, Maddela Rambabu, M. Praveen, Shrikant Charde, Madhusmita Sahu, Prasun Chakrabarti, Bui Thanh Hung, Martin Margala, B. Unhelkar, A. Narayanankutty","doi":"10.1002/sscp.202300131","DOIUrl":"https://doi.org/10.1002/sscp.202300131","url":null,"abstract":"Dacomitinib, a quinazoline compound, exhibits antineoplastic activity against brain metastasis activities in non‐small cell lung cancer and the central nervous system. In this study, the liquid–liquid extraction method with high‐performance liquid chromatography and tandem mass spectrometry detection method was established and validated for the determination of Dacomitinib in human plasma. Plasma samples were prepared and chromatographic separation was achieved on analytical column Discovery C18 (10 cm × 4.6 mm, 5 μm) with gradient elutes at a flow rate of 0.8 mL/min, using a mobile phase consisting of acetonitrile and ammonium formate. Dacomitinib and dacomitinib D10 (internal standard) were detected by multiple reactions. The method was fully validated according to the United States Food and Drug Administration guidelines. The calibration curve was linear with an excellent correlation coefficient (r2 ˂ 0.99). The method validation steps such as carry‐over, matrix effect, extraction recovery, dilution effect, intra‐inter accuracy, and precision were found within acceptable limits. The method was then applied to a pharmacokinetic study in human plasma. After oral administration, the plasma concentration in different volunteers reached 0.5–250.01 ng/mL. The result established can be applied to the estimation of drugs in human plasma.","PeriodicalId":21639,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138590804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel stability indicating mass compatible reversed‐phase high‐performance liquid chromatography for the determination of Diroximel fumarate and its related impurities 用于测定富马酸双羟萘酸及其相关杂质的新型稳定性指示质量兼容反相高效液相色谱法

IF 1.1

SEPARATION SCIENCE PLUS Pub Date : 2023-12-07 DOI: 10.1002/sscp.202300171

Shikha Shah, C. Kothari, Krishna Bhalodi, Misari Patel

{"title":"A novel stability indicating mass compatible reversed‐phase high‐performance liquid chromatography for the determination of Diroximel fumarate and its related impurities","authors":"Shikha Shah, C. Kothari, Krishna Bhalodi, Misari Patel","doi":"10.1002/sscp.202300171","DOIUrl":"https://doi.org/10.1002/sscp.202300171","url":null,"abstract":"Diroximel fumarate (DRF) is a novel oral fumarate used in the treatment of multiple sclerosis. Comprehending DRF's stability behavior in different degradation conditions is crucial and has been studied under photolysis, oxidative, hydrolysis, and thermal conditions. Nucleosil C18 column (250 × 4.6 mm, 5 μm) was employed to achieve drug separation, with a mobile phase comprising of water and acetonitrile (65:35, v/v). The flow rate was 1 mL/min and detection was performed at 210 nm using a photodiode array detector. The fully validated reversed phase‐high‐performance liquid chromatography stability method as per the International Council for Harmonization Q2 R1 demonstrated excellent selectivity, accuracy, and precision with good sensitivity. During the analysis, two prominent degradant product peaks of DRF were identified across all stress conditions. The usage of liquid chromatography‐mass spectrometry compatible solvents in the mobile phase permits further characterization of DRF and its degradants peak. Significant levels of degradation were found in alkaline and acidic hydroxide, peroxide, photolytic, and thermal conditions with hydrolysis being postulated as a possible mechanism of degradation. The fully validated stability‐indicating analytical method can be used for routine quality control and stability testing of DRF, requiring storage in a protected primary container, as it is sensitive to light and moisture.","PeriodicalId":21639,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138592695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous estimation of andrographolide, apigenin, apocynin, and gallic acid by high‐performance thin layer chromatography method with Greenness quality by design approach 采用绿色质量设计方法的高效薄层色谱法同时测定穿心莲内酯、芹菜素、芹菜苷和没食子酸

IF 1.1

SEPARATION SCIENCE PLUS Pub Date : 2023-12-07 DOI: 10.1002/sscp.202300109

Shweta Mevada, Saurabh Shukla, Harsha Patel

{"title":"Simultaneous estimation of andrographolide, apigenin, apocynin, and gallic acid by high‐performance thin layer chromatography method with Greenness quality by design approach","authors":"Shweta Mevada, Saurabh Shukla, Harsha Patel","doi":"10.1002/sscp.202300109","DOIUrl":"https://doi.org/10.1002/sscp.202300109","url":null,"abstract":"People's health benefits from andrographolide (ANDRO), apigenin (API), gallic acid (GA), and apocynin (APO) because they aid in the treatment of liver illness. The study mainly illustrated that a green approach is essential in the medical sector as well as the herbal sector with the help of “Green Analytical quality by design”. The mobile phase combination Dichloromethane: Ethyl acetate: Formic acid (10:8:0.4)v/v/v was optimized with the aid of an analytical quality‐by‐design approach. The absorbance mode at 254 nm was chosen for densitometric analysis as it gives a compact spot of ANDRO, API, GA, and APO at RF values of 0.24, 0.55, 0.17, and 0.68. Linearity was obtained in the range of 200–800 ng for all four markers. After retardation factor, ANDRO, APO, API, and GA from the tablet were found 0.0433, 0.0020, 0.1356, and 0.0015 mg, respectively. The validation parameter of R2 value ranged from 0.998, 0.999, 0.996, and 0.998 for all four ANDRO, API, GA, and APO. The study's findings revealed that the “high‐performance thin layer chromatography” technology is an environmentally friendly analytical design that helps to maintain the stability of the medication. It was also acknowledged as a quality design and a fresh idea in the pharmaceutical industry.","PeriodicalId":21639,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138591229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of an effervescence‐assisted dispersive liquid phase microextraction using a binary solvent as a simultaneous extraction and preconcentration approach specialized for the analysis of pesticides in grape juice without dilution 开发一种使用二元溶剂的起泡辅助分散液相微萃取法，作为一种无需稀释即可同时进行萃取和预浓缩的分析葡萄汁中农药的专用方法

IF 1.1

SEPARATION SCIENCE PLUS Pub Date : 2023-12-04 DOI: 10.1002/sscp.202300195

M. Farajzadeh, Ali Hoboobi, S. Pezhhanfar, M. Mogaddam

{"title":"Development of an effervescence‐assisted dispersive liquid phase microextraction using a binary solvent as a simultaneous extraction and preconcentration approach specialized for the analysis of pesticides in grape juice without dilution","authors":"M. Farajzadeh, Ali Hoboobi, S. Pezhhanfar, M. Mogaddam","doi":"10.1002/sscp.202300195","DOIUrl":"https://doi.org/10.1002/sscp.202300195","url":null,"abstract":"In this study, a swift and economical extraction and preconcentration method called effervescence‐assisted dispersive liquid‐phase microextraction was developed to analyze the pesticide content of different grape juices. To propel the microextraction method, the sample was transferred to a conical bottom glass test tube, and ammonium chloride was added and vortexed to dissolve. Then, sodium bicarbonate and a mixture of 1,2‐dibromoethane and n‐hexane were added. The tube was located in a water bath and the produced carbon dioxide bubbles dispersed the extraction solvent into the sample. Finally, it was centrifuged and one microliter of the sedimented phase was injected into a gas chromatograph equipped with a flame ionization detector. Under the optimum conditions, the limits of detection and quantification ranged from 0.9 to 4.1 and 3.0 to 13.6 μg/L, respectively. Extraction recoveries and enrichment factors were achieved in the ranges of 36%–80% and 180%–404%, respectively. The inter‐ and intra‐day relative standard deviations were in the ranges of 3.4%–10.6% and 2.6%–9.7%, respectively. Also, the coefficients of determination in the calibration curves were ≥0.990. Short extraction time, no sorbent or costly apparatuses, using inexpensive chemicals, applying microliter levels of the extractant, and no dilution of the real samples are the highlights of the study.","PeriodicalId":21639,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138602153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemical study: Fragmentation patterns of flavonoid‐C‐glycosides in the enriched flavonoids from corn silk using high‐efficiency ultra‐high‐performance liquid chromatography combined with quadrupole time‐of‐flight mass spectrometry 植物化学研究：高效超高效液相色谱-四极杆飞行时间质谱法测定玉米丝富集黄酮类化合物中黄酮-C-糖苷的碎片模式

IF 1.1

SEPARATION SCIENCE PLUS Pub Date : 2023-11-22 DOI: 10.1002/sscp.202300156

Yumei Wang, Meiling Gu, Jialin Mao, Jianhua Liu, Songjie Fan, Honglian Zhang, Hongzhou Bu, Qi Liu

{"title":"Phytochemical study: Fragmentation patterns of flavonoid‐C‐glycosides in the enriched flavonoids from corn silk using high‐efficiency ultra‐high‐performance liquid chromatography combined with quadrupole time‐of‐flight mass spectrometry","authors":"Yumei Wang, Meiling Gu, Jialin Mao, Jianhua Liu, Songjie Fan, Honglian Zhang, Hongzhou Bu, Qi Liu","doi":"10.1002/sscp.202300156","DOIUrl":"https://doi.org/10.1002/sscp.202300156","url":null,"abstract":"Flavonoid‐C‐glycosides with various pharmacological actions were abundant in corn silk, and fragmentation in mass spectra of flavonoid‐C‐glycosides compounds was particularly important. This paper aimed to supervise the fragmentation patterns of flavonoid‐C‐glycosides in the enriched flavonoids from corn silk by high‐efficiency ultra‐high‐performance liquid chromatography combined with a quadrupole time‐of‐flight mass spectrometry approach. Therefore, the flavonoids were extracted by heating reflux and then purified based on D101 macroporous resin. Thirty‐five flavonoids were triumphantly identified in the purified product. Among them, 14 were flavonoid‐C‐glycosides, seven of them owned luteolin mother nuclei (luteolin‐C‐glycosides) and seven of them owned apigenin mother nuclei (apigenin‐C‐glycosides). Importantly, the diagnostic product ions of luteolin‐C‐glycosides including m/z (431, 413, 395, 369, 367, 313, and 299) and m/z (429, 411, 393, 367, 365, 311, and 297), and the characteristic product ions of apigenin‐C‐glycosides covered m/z (353, 339, and 299) and m/z (339, 327, and 309). In addition, diagnostic H2O neutral loss and glycosyl craking were familiar, and the difference values among product ions mainly contained 40, 60, 70, 80, 90, 100, and 120 Da. Thus, the fragmentations of luteolin‐C‐glycosides and apigenin‐C‐glycosides identified in corn silk were with proper regularity. The obtained fragmentation patterns provided rapid recognition and meaningful guidance for the purification study of flavonoid‐C‐glycosides.","PeriodicalId":21639,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139247380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid determination of multiple components in herbal medicine using a single reference compound by high‐performance liquid chromatography at equal absorption wavelength: Case study of Magnoliae officinalis cortex 等吸收波长高效液相色谱法利用单一参比化合物快速测定中药中的多种成分：厚朴皮的案例研究

IF 1.1

SEPARATION SCIENCE PLUS Pub Date : 2023-11-20 DOI: 10.1002/sscp.202300071

Zhengming Qian, Jing Chen, Qing-Qing Lei, Guoying Tan, Yuansheng Zou, Gang Peng, Juying Xie, Wenqing Li

{"title":"Rapid determination of multiple components in herbal medicine using a single reference compound by high‐performance liquid chromatography at equal absorption wavelength: Case study of Magnoliae officinalis cortex","authors":"Zhengming Qian, Jing Chen, Qing-Qing Lei, Guoying Tan, Yuansheng Zou, Gang Peng, Juying Xie, Wenqing Li","doi":"10.1002/sscp.202300071","DOIUrl":"https://doi.org/10.1002/sscp.202300071","url":null,"abstract":"Herbal medicine is a complex system containing numerous bioactive components. Evaluating the quality of herbs often requires analyzing multiple components using high‐performance liquid chromatography (HPLC). However, existing HPLC methods are time‐consuming and consume large amounts of reference compounds. This study, an ultra‐rapid and green assay method for the determination of honokiol and magnolol in Magnoliae Officinalis Cortex using a single reference compound was developed by HPLC at equal absorption wavelength (EAW). The sample was prepared using ultrasonic‐assisted matrix solid‐phase dispersion and separated with an eco‐friendly mobile phase on the Poroshell C18 column. The EAW of homokiol and magnolol was chosen as the detecting wavelength (247 nm). The contents of honokiol and magnolol in six Magnoliae Officinalis Cortex samples obtained by the developed method with a single marker and the external standard method with two markers were comparable. Additionally, the developed HPLC process only needed 4.55 mL green organic solution (ethanol) and 2.5 min, which included sample extraction and separation. The developed HPLC EAW method was ultra‐fast, green, and reference compound saving, which was an improved quality control method of Magnoliae Officinalis Cortex and would serve as an example for the determination of multiple components in other herbal medicines using a single reference compound.","PeriodicalId":21639,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139258482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacokinetics, tissue distribution, and excretion study of Neocryptotanshinone in rats using liquid chromatography‐tandem mass spectrometry 利用液相色谱-串联质谱法研究新隐丹参酮在大鼠体内的药代动力学、组织分布和排泄情况

IF 1.1

SEPARATION SCIENCE PLUS Pub Date : 2023-11-20 DOI: 10.1002/sscp.202300095

Chang Liu, Jia‐Ying Zhang, Yong‐Ming Wang, Xiao‐Ling Cheng, Li‐Yuan Qu, Yan‐Li Wang, Yi‐Fan Sun, Yong Wang, Zhi‐Min Du, Wei Wang, Jin‐Hui Wang

{"title":"Pharmacokinetics, tissue distribution, and excretion study of Neocryptotanshinone in rats using liquid chromatography‐tandem mass spectrometry","authors":"Chang Liu, Jia‐Ying Zhang, Yong‐Ming Wang, Xiao‐Ling Cheng, Li‐Yuan Qu, Yan‐Li Wang, Yi‐Fan Sun, Yong Wang, Zhi‐Min Du, Wei Wang, Jin‐Hui Wang","doi":"10.1002/sscp.202300095","DOIUrl":"https://doi.org/10.1002/sscp.202300095","url":null,"abstract":"Salvia miltiorrhiza Bunge is a Traditional Chinese Medicine used for the treatment of diseases related to the heart and liver. Owing to the abundance of salvianolic acids and tanshinones, S. miltiorrhiza exhibits various pharmacological activities, such as stimulating blood circulation, eliminating blood stasis, relieving menorrhea and pain, and improving polydipsia. Neocryptotanshinone (NCTS) is one of the tanshinones isolated from S. miltiorrhiza and exhibits anti‐inflammatory, hypoglycemic, and preventive effects against cardiovascular diseases; however, its pharmacokinetic properties have not been reported to date. Therefore, a simple and reliable LC–MS/MS method was developed to determine absorption, distribution, and excretion of NCTS in rat physiological samples. We demonstrated that NCTS had high oral absolute bioavailability of 85.96% ± 25.32%, and it was rapidly distributed in both the heart and brain, and the fecal excretion of NCTS was the highest. This study provides a theoretical basis for further development of NCTS as a potential clinical candidate.","PeriodicalId":21639,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139259564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential of three different adsorbents in solid‐phase extraction of antihistaminic and antimigraine drugs from water samples using ultra‐high‐performance liquid chromatography‐ultraviolet analysis 三种不同吸附剂在利用超高效液相色谱-紫外分析法固相萃取水样中抗组胺药物和抗偏头痛药物中的潜力

IF 1.1

SEPARATION SCIENCE PLUS Pub Date : 2023-11-20 DOI: 10.1002/sscp.202300194

S. Jebali, A. Labidi, R. B. Sghaier, L. Latrous, Adel Megriche

{"title":"The potential of three different adsorbents in solid‐phase extraction of antihistaminic and antimigraine drugs from water samples using ultra‐high‐performance liquid chromatography‐ultraviolet analysis","authors":"S. Jebali, A. Labidi, R. B. Sghaier, L. Latrous, Adel Megriche","doi":"10.1002/sscp.202300194","DOIUrl":"https://doi.org/10.1002/sscp.202300194","url":null,"abstract":"In this study, eight pharmaceuticals belonging to two classes, antihistaminic and antimigraine, were considered. Ultra‐performance liquid chromatography‐diode array detection was set up and gradient elution was used. The mobile phase was composed of acetonitrile and trifluoroacetic acid solution. Chromatographic separation was carried out with a run time of 9 min. The preconcentration of eight studied solutes was realized by micro solid‐phase extraction. Multiwalled carbon nanotubes, cetyltrimethylammonium bromide‐titanium dioxide nanotubes, and C‐18 bonded silica solid‐phase extraction (SPE) sorbents were evaluated and compared concerning recoveries. The results of the optimization of factors affecting the extraction efficiency including sample volume, the elution solvent type, and its volume are reported. For validation of the analytical procedure, specificity, linearity, precision, accuracy, limit of detection, and limit of quantification were evaluated. The developed method was successfully applied to the analysis of wastewater treatment samples. The extraction recoveries of pharmaceutical substances were above 81.23% for the three SPE sorbents. The extraction method was selective and the detection and quantification limits were between 0.004–0.02 and 0.01–0.06 μg/L, respectively. All the results indicated the potential application of the three studied SPE sorbents for the extraction of selected pharmaceuticals from wastewater.","PeriodicalId":21639,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139254985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0