ScienceRise: Biological Science最新文献

{"title":"Bacterial risks and determination of critical control points at the industrial production of chicken edible egg","authors":"Denys Demianenko","doi":"10.15587/2519-8025.2023.285116","DOIUrl":"https://doi.org/10.15587/2519-8025.2023.285116","url":null,"abstract":"The aim: study the bacterial risks and to determine the control critical points at the industrial production of chicken edible egg.
 Materials and methods. The first stage of our research was to study the degree of risk of contamination by opportunistic and pathogenic microflora at all stages of production of chicken edible eggs according to ISO 22000:2007. In order to achieve the goal, bacteriological research was conducted on pathological material from day-old chickens and adult birds, the content of marketable eggs from birds of different age groups; as well as washed from the equipment of poultry farms, the repair young poultry department, the industrial herd department, egg sorting and certification workshops, from the surface of the egg, from the working surfaces of special vehicles. Bacterial contamination of air, droppings, bedding, complete feed was studied. Bacteriological studies were carried out according to generally accepted schemes, using accumulative, selective and differential diagnostic media (heptadecyl sulfate agar, endo, xylose-lysine agar, differentiated agar with diamond green, Muller-Hinton). The sampling was carried out using universal sterile applicators \"Voles\".
 The next stage was to analyze the results of bacteriological studies and determine the basic list of criteria for creating critical control points (CCPs) for the further development of a risk management scheme for the bacterial biosafety of edible eggs according to HACCP principles.
 Results. Isolation of S. aureus from the heart and lungs, E. coli, P. aeruginosa, Enterobacter spp, Enterococcus spp - from the intestines of day-old chickens and litter - indicate violations of veterinary and sanitary standards in the hatchery and the low quality of disinfection before the placement of day-old young birds, as well as non-observance of veterinary and sanitary norms for the transportation regime of day-old young birds. Isolation of E. coli, Enterobacter spp, Klebsiella spp.Str. zymogenes from compound feed, and E. coli, Enterobacter spp, Shigella spp. in washings from the equipment of the feed mill is evidence of insufficient veterinary and sanitary control of incoming raw materials, compound feed, as well as poorly carried out disinfection of equipment and specialized vehicles.When examining objects from the production line, E. coli, Enterobacter spp., P. aeruginosa, and S. epiderrmidis were most often isolated. From the pathological material during the bacteriological examination, microflora of the genus E. coli, Streptococcus spp., S. aureus (most often pathogenic serotypes) prevailed. As a result of the analysis of the conducted studies, we determined a basic list of criteria for creating a CCP and developing a risk management scheme for the bacterial biosafety of edible eggs according to the principles of HACCP.
 Conclusions. As a result of the bacteriological monitoring of objects of the technological cycle of chicken edible egg production,","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136365310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orthodontic correction in rodents and hare-like animals: principles and methods of treatment","authors":"Hanna Stepanenko, O. Siehodin","doi":"10.15587/2519-8025.2023.276319","DOIUrl":"https://doi.org/10.15587/2519-8025.2023.276319","url":null,"abstract":"The aim: Our particular interest in this study is not only the ability to extrapolate the experience of orthodontics of humane medicine for effective orthodontic correction in representatives of the animal world, but also the possibility of using teleroentgenometry and craniometry to study the skull of rodents and hare-like animals for the early preclinical diagnosis of dental disease. \u0000Materials and methods. The data of teleroentgenography (TRG), cranio- and gnatometry, biochemistry of connective tissue (GAG, GP, HST), fluoroscopy, densitometric parameters for early subclinical detection of dental disease in chinchillas (Chinchilla lanigera (n=20)), guinea pigs (Cavia porcellus (n=48)) and rabbits (Oryctolagus cuniculus(n=52)) are presented. All stages of the effective correction of mesial occlusion of incisors in rabbits (N=5) and dystropia of premolars in guinea pigs (N=5) are described. The camputation of efforts and points of their application that are necessary to move the tooth of the ellodont type is carried out. There are given the sequential stages of creating a dental imprint or 3D models, as well as the manufacture of fixed orthodontic structures, including an elastophore, orthodontic buttons with an Enlight Ormco fixation for incisors; and individual extraoral devices with expanding screws for premolars are presented. \u0000Results. Namely, among animals with dental disease, the following anatomical characteristics reliably took place. The basal angle of inclination of the base of the jaws to each other characterizing the vertical position of the jaws increased by 11 %; the body of the lower jaw shortened by 18 %; the height of the branches of the jaw increased by 17.5, and the mandibular angle, which is measured between the tangents to the lower edge of the lower jaw and the back surface of its branches, increased by 6 %. These data must be considered together with a reliable densitometric decrease in bone density and changes of biochemical components of the connective tissue in the blood serum. An analysis of bone strength of rabbits and guinea pigs is given in Tab. 2, which shows that the bone marrow of animals with dental history is statistically significantly different from the strength of animal bones without such among patients of rabbits and guinea pigs (p = 0.012 and p = 0.024, respectively). Thus, the method of program densitometry can be used to quantify the severity of metabolic disorders in the bone tissue to predict the further course of the reparative process, to appoint adequate pharmacological correction and to control the evaluation of therapeutic measures. \u0000Conclusions. The study of dental pathology of rodents and hare-like animals using densitometric, craniometric and biochemical methods allows detection of disorders in the early preclinical stage. And the extrapolation of the experience of humane orthodontics solves the issue of correcting the occlusion of these types of animals to restore the possibility of self-feed","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73919234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The consequences of phytoestrogenization of the father and the effects of phytoestrogens during puberty for male offspring","authors":"N. Seliukova, Yevgenia Korenieva, D. Morozenko, Yevheniia Vashchyk, Rymma Yeromenko, O. Matviichuk, Anatolii Matviichuk, Oleg Gladchenko","doi":"10.15587/2519-8025.2023.275068","DOIUrl":"https://doi.org/10.15587/2519-8025.2023.275068","url":null,"abstract":"Exposure to phytoestrogens (PE) during prepuberty and puberty can modulate the functioning of the reproductive axis, causing irreversible damage to reproductive programming. \u0000The aim of the study was to investigate the state of the reproductive system of male offspring of adult rats that were exposed to phytoestrogens in the pubertal period of ontogenesis. \u0000Materials and methods. The work was performed on adult male and female Wistar rats and their male offspring. In the experiment, the biological effect of PE was studied when applying a dose of 20 mg/kg of body weight for 30 days to the father and/or offspring of puberty age starting from the 45th day of postnatal life. Upon reaching the age of six months, male offspring of all studied groups were examined for reproductive function. \u0000Results. The effect of estrogen-like substances on male reproductive function is manifested not only under the conditions of their intake in the critical periods of the embryonic and postnatal periods, but also, even when acting on the germ cells of parents. In male offspring, androgen secretion is disturbed, the hormonal status changes in the direction of hyperestrogenization, fertility decreases due to the reduced quality of germ cells against the background of a normal spermogram. \u0000Conclusion. The reproductive function of sexually mature male offspring of a phytoestrogenized father who received a mixture of phytoestrogens during puberty is characterized by differences in sexual behavior, a decrease in the reproductive potential of males, which occurs due to a decrease in the share of effective fertilization, which indicates negative changes in spermatozoa, the development of which took place in conditions of absolute and relative hyperestrogeny. This indicates that phytoestrogens, as an environmental factor, have adverse consequences not only for individuals who directly use them, but also for their male offspring","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81861370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The manifestation of features of opioids and narcotics using by drug addicted people and the role of replacement pharmacotherapy in treatment and recovery of the patient","authors":"N. Sulashvili, N. Abuladze, Margarita Beglaryan, Jilda Cheishvili, Ada (Adel) Tadevosyan, Marika Sulashvil","doi":"10.15587/2519-8025.2023.276374","DOIUrl":"https://doi.org/10.15587/2519-8025.2023.276374","url":null,"abstract":"Aim of the research was to study and analyze the features of opioids and narcotics using by drug addicted people and the role of replacement drugs in treatment and recovery of the patient. \u0000Materials and methods: We conducted a systematic review and meta-analysis of studies. Were used Systematic literature reviews and meta-analyses. The material of the article was data from the scientific literature, processed and analyzed by generalization and systematization. The scientific research ensues the fundamentals of assessment development of significant reviews. The ensuing databases were used: (for searching considerable literature to study and analyze the features of opioids and narcotics using by drug addiction people and the role of replacement drugs in treatment and recovery of the patient) Pub Med, Web of Science, Clinical key, Tomson Routers, Google Scholar, Cochrane Library, and Elsevier bases. Additionally studied national and internationals policy and guidelines and also grey literature. \u0000Results: Addiction is well known to be associated with its high level of physical and mental disorders. Most chronic chemical dependencies of drug addiction are also associated with its very high mortality rates, estimated to be several times those of the non-clinical population. Since addictive substances are known to disrupt cell growth and cell division, it can be assumed, that they particularly affect dividing cells, such as stem cell pools and progenitor cells. It is also known, that they either individually or in combination potentiate apoptosis, i.e., contribute to this effect. The medicine of aging in recent times has become an independent scientific discipline. The cellular aging hypothesis suggests the aging phenotype. The organism is associated with cellular correlates of age associated changes including cell loss, reduced cell velocity, renewal and more aging, negligible functional and non-replicating cells in tissues. So, the anti-growth effects of drug addiction can reasonably occur throughout the body. Expect signs of accelerated aging to be evident. One would expect such a putative progeroid effect to occur subject to increased morbidity and mortality rates, clinically observed almost identically in drug addicts as is the case in the geriatric population. In this connection there are various changes, consideration of all clinical aspects’ expression of this general toxicology hypothesis of opioids is needed. \u0000Conclusion: Pharmacological management of drug use should be only one component of treatment for drug needs, tailored to a comprehensive needs assessment of the child or young person, carried out in conjunction with appropriate psychological therapy and mental health interventions, and in the context of a clear and applied approach to the clinical management system. Caution is required when leaving these establishments due to the risk of overdose and in the transition to adult services. Physicians should carefully consider the degree of","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77681212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Some biological, behavioral and social aspects of the perfumery use in the Ukrainian population sample (Part 2. Education level associations)","authors":"O. Filiptsova, O. Naboka, S. Bobro, O. Bashura, Yuliia Osypenko","doi":"10.15587/2519-8025.2023.277216","DOIUrl":"https://doi.org/10.15587/2519-8025.2023.277216","url":null,"abstract":"The aim. The study represents a continuation of the previous one, and provide various aspects related to the use of perfumery, which can be characterized by certain associations with the education level of Ukrainian consumers. Factors that have direct or indirect biological and medical significance and, accordingly, may have a potential impact on the safety of the perfume products use by humans, were selected for the analysis. \u0000Materials and methods. The analysis included information about 124 individuals living in Ukraine. The respondents answered the questions of the questionnaire developed regarding some aspects of the perfumery use. The Pearson chi-squared (χ2) test was used to analyze the relationships between qualitative characteristics. \u0000Results and discussion. Most of the studied aspects related to the perfumes use demonstrate associations, caused by the dependence of the education level on age. The analysis revealed that individuals with any education most often (about half of the cases or more) choose a perfume based on its scent. The study showed that the majority of respondents believe that the price of a perfume depends on a combination of factors, namely the composition of the products, the brand and the cost of the packaging. More than 80 % of people, regardless of the education level, believe that the persistence of a perfume is definitely a sign of quality. The most common opinion among people of all education levels was that non-original perfumes can have a more negative effect on people's health than original products. The \"lipstick effect\" in relation to perfume products (demand for items of \"affordable luxury\" in times of economic crises and wars) was observed. \u0000Conclusions. The study supplemented the previously found associations of age and some biological and behavioral aspects of perfume use among the population of Ukraine with data on the presence or absence of such associations in relation to the education level","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"208 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74591279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"II. Morphometry of wings of worker bees of the subspecies Apis mellifera mellifera L. (Polissya population of Zhytomyr region)","authors":"O. Galatyuk, V. Yarovets, V. Babenko, V. Cherevatov, B. Gutyj, Andrii Grigorenko, M. Strilchuk, Ihor Stolyar","doi":"10.15587/2519-8025.2023.275588","DOIUrl":"https://doi.org/10.15587/2519-8025.2023.275588","url":null,"abstract":"The uncontrolled spread of the subspecies A. m. carnica, A. m. ligustica, and A. m. caucasica has led to a reduction in the areas of pure \"dark forest bees\" populations belonging to the subspecies A. m. mellifera in their natural habitats within Ukraine. Due to the need to use dark forest bees in breeding, it became necessary to identify the locations of individual populations of bees belonging to the A. m. mellifera subspecies. \u0000The purpose of the work is to create an accessible and at the same time complete methodology for classifying bee wing phenotypes, which would make it possible to determine the probable breed of worker bees, the type and degree of hybridization of the main breed with impurities, and to identify \"purebred\" bee families by the wing phenotype suitable for further breeding. \u0000Material and methods of research: Using discriminant analysis of data, at the first stage of the study, 1500 wings of bee families were classified using 8 features: Ci, Dbi, Disc.sh, Pci, Ri, Сі.3, Сі.2, Сi.2.1, for which there was preliminary information about the possible belonging of the wing phenotype to the subspecies A. m. mellifera or its hybrids. At the second stage, additional 1212 wings of bee colonies were studied, about which there were doubts about their breed. \u0000Results of research and discussion: The wings are reliably divided into four clusters, indicating the presence of four sufficiently distinct groups among the studied wings in terms of phenotype. \u0000Conclusions and prospects for further research: A classification model has been created that allows for effective discrimination of the wings of working bees of bee colonies in Ukraine, the subspecies A. m. mellifera. Phenotypic values of indices of four Polissia micro-populations of bees, used as reference standards for possible hybridization detection, have been established, which can serve as standards in future research. Four colonies have been found, whose queens produce bees of the A. m. mellifera type of the Polissia population, and three colonies whose queens produce bees of the A. m. macedonica hybrid and can be used for further selection work","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83739800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the influence of dry extracts of bupleurum aureum and Salsola collina L. on the antimicrobial effect of co-trimoxazole","authors":"O. Naboka, A. Kotvitska, N. Filimonova, A. Glushchenko, O. Filiptsova, A. Volkova","doi":"10.15587/2519-8025.2023.275942","DOIUrl":"https://doi.org/10.15587/2519-8025.2023.275942","url":null,"abstract":"Scientific data on the pharmacodynamics of dry extracts of Bupleurum aureum and Salsola collina L. based on the results of studying the antimicrobial effect and the similar effect of co-trimoxazole when they are used together have been supplemented. The investigated phytoextracts do not show antimicrobial properties, but they do not change the antimicrobial effect of co-trimoxazole when they are used in combination. \u0000The aim of the study was to experimentally investigate the antimicrobial effect of extracts of Bupleurum aureum and Salsola collina L. and establish the possible antagonistic effect of these extracts on the antimicrobial drug co-trimoxazole when used together. \u0000Materials and methods. The research was conducted in May 2016. Screening of the antimicrobial effect of extracts of Bupleurum aureum and Salsola collina L. and establishing of the possible antagonistic effect of these extracts on the antimicrobial drug co-trimoxazole when they are used together was carried out in the laboratory of the Department of Microbiology of the National Pharmaceutical University, which has a certificate of attestation 045/14 dated 28.10.2014. For determination of antimicrobial activity, the agar diffusion method (\"well\" method), which is based on the ability of medicinal substances to penetrate the agar layer, was used. A set of reference strains of microorganisms was used: S. aureus ATCC 6538, E. coli ATCC 8739, P. aeruginosa ATCC 9027, B. subtilis ATCC 6633, C. albicans ATCC 10231. Petri dishes were filled with two layers of solid nutrient medium. The lower layer - 10 ml of melted \"cold\" AGV agar (medium No. 3), the upper layer - nutrient medium for the corresponding test strain. After cooling the lower layer of agar, three thin-walled steel cylinders (inner diameter - 6.0±0.1 mm, height - 10.0±0.1 mm) were placed on it at an equal distance from each other and from the edge of the cup. The top layer was poured around the cylinders - 13.5 ml of agar, melted and cooled to 45-48°С, mixed with the seed dose of the test microorganism (1.5 ml of microbial suspension, the concentration corresponding to the type of microorganism). After cooling the upper layer of agar, the cylinders were removed with sterile tweezers and 0.25-0.3 ml of the studied drug was added to the resulting wells. The results were recorded after 24 h by measuring the zone of growth inhibition, including the diameter of the wells. Measurements were made with an accuracy of 1 mm, while focusing on the complete absence of visible growth. \u0000The obtained data were analyzed using the methods of variational statistics. The significance level is p<0.05. The studied plant extracts of Bupleurum aureum (aqueous and alcoholic) and Salsola collina L. (aqueous and alcoholic) were used in doses of 0.005 mg/ml and 0.01 mg/ml, which corresponded to doses of 5 mg/kg and 10 mg/kg. Experimental data were also processed by parametric (Newman-Keuls) and non-parametric (Mann-Whitney) methods of variational stat","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91316558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The pathogenetic role of glycoproteins and proteoglycans in dog glomerulonephritis","authors":"D. Morozenko, Yevheniia Vashchyk, A. Zakhariev, Dmytro Berezhnyi, N. Seliukova, K. Gliebova","doi":"10.15587/2519-8025.2022.266237","DOIUrl":"https://doi.org/10.15587/2519-8025.2022.266237","url":null,"abstract":"The aim: to determine the main pathogenetic links of glomerulonephritis in dogs with the participation of connective tissue biopolymers. \u0000Materials and methods The research was conducted by analyzing the sources of scientific literature (PubMed, Elsevier, electronic resources of the V. I. Vernadskyi National Library) and the clinical experience of the authors, thanks to which a scheme of the pathogenesis of glomerulonephritis in dogs with the participation of connective tissue biopolymers was developed. \u0000Results. According to the results of our research, it was established, that chronic glomerulonephritis in dogs is accompanied by neutrophilia, lymphocytosis, as well as urinary and nephrotic syndromes, the progression of which causes a violation of the functional state of the kidneys and liver. Sick dogs develop a nephrotic symptom complex – persistent proteinuria, hypoalbuminemia, dysproteinemia, and hyperlipidemia. Clinically, edema was not observed, because it is known, that in dogs they are rarely detected with nephrotic syndrome. Inflammation in the kidneys is accompanied by an increase in the concentration of glycoproteins and sialic acids in the blood serum. After treatment, there was a decrease in the inflammatory process in the kidneys, which was manifested by a decrease in neutrophilia and lymphocytosis, as well as the content of glycoproteins and sialic acids in the blood serum. The content of total chondroitin sulfates and the fractional composition of glycosaminoglycans did not change, and the level of excretion of oxyproline and uronic acids decreased compared to the indicators before the start of treatment. This, in our opinion, is due to the slowing down of fibrotic processes in the kidneys. Thus, biochemical indicators of the state of connective tissue in the blood serum and urine of dogs with glomerulonephritis allowed us to evaluate the functional state of the kidneys for their inflammation (oxyproline and uronic acids), as well as to determine the violation of proteoglycan synthesis in nephrotic syndrome. \u0000Conclusions. For glomerulonephritis in dogs on the background of depression, decreased appetite, polydipsia, pain during palpation in the lumbar region, periodic vomiting, neutrophilic leukocytosis, lymphocytosis, nephrotic syndrome (hypoalbuminemia, increase in α2- and β-globulins, cholesterol, β-lipoproteins), growth activity of ALT, AST, and alkaline phosphatase, a decrease in Veltman's test, hyperazotemia, hyperphosphatemia, proteinuria, microhematuria, and cylindruria, there is an increase in the content of glycoproteins, sialic acids, and chondroitin sulfates, as well as heparan sulfate in the blood serum of dogs. The increase in the blood serum of patients with canine glomerulonephritis, as a marker of connective tissue – glycoproteins, sialic acids, chondroitinsulfates, and urinary excretion of oxyproline and uronic acids is due to inflammatory and fibrotic changes in the basal membranes of the kidney glomeruli","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"77 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82300468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diabetes mellitus in domestic cats: clinical cases from veterinary practice","authors":"D. Morozenko, Yevheniia Vashchyk, A. Zakhariev, N. Seliukova, Dmytro Berezhnyi, K. Gliebova","doi":"10.15587/2519-8025.2022.266536","DOIUrl":"https://doi.org/10.15587/2519-8025.2022.266536","url":null,"abstract":"The aim: to analyze clinical cases of diabetes mellitus in cats and establish the effectiveness of clinical and laboratory research and treatment of animals with the help of insulin therapy. \u0000Materials and methods. The material for the study were domestic cats admitted to the veterinary medicine clinic \"Doctor Vet\" (Lviv). \u0000Results. Clinical case 1. Cat Bonya, age 10 years, body weight 4 kg. symptoms: polyuria/polydipsia and weight loss. Examination: cachexia, weakness, pale mucous membranes, unsteady gait. Blood glucose - 20.4 mmol/l, urine analysis - pH=6.0, glucose ++++, no ketones detected. Diagnosis: diabetes. For glycemic control - caninsulin at 0.25 IU/kg every 12 hours. After the start of insulin therapy, the animal's condition improved, after 2 months - an attack of hypoglycemia, blood glucose 3.0 mmol/l. The introduction of insulin was stopped for 10 days, after 10 days the cat's appetite is normal, polyuria and polydipsia are not observed, body weight gain is 300 g, blood glucose without insulin therapy is 8.0 mmol/l. \u0000Clinical case 2. Cat Eva, age 12 years, body weight 6.7 kg, symptoms: polyuria/polydipsia during the last two weeks, increased appetite, diet - raw meat, meatballs, buckwheat porridge, boiled carrots, beets. Blood glucose 22.9 mmol/l, urine analysis – pH = 6.0, glucose ++++, ketones +. Diagnosis: diabetes. Treatment: Lantus 2 IU subcutaneously every 12 hours. After the start of insulin therapy, the condition improved, after 2 months, blood glucose was 9.00 - 13.0 mmol/l; 15.00 – 8.0 mmol/l, 21.00 – 12.0 mmol/l, glucose and ketones are absent in the urine. \u0000Conclusions. In the first clinical case, the cat there was a decrease in demand in insulin “Caninsulin” on the background of insulin therapy. In the second clinical case, insulin therapy with the help of \"Lantus\" insulin allows you to reach a level of glycemia at which diabetes stops progressing, the clinical condition of the animal normalizes, which indicates the effectiveness of the prescribed treatment. Thus, we can say that diabetes in small domestic animals is a rather severe endocrine pathology, which has typical clinical and laboratory symptoms and can be successfully treated with the help of a complex medical and diagnostic approach, based on insulin therapy","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78352343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research of the influence of Melanizol pessaries on background of “chemical” vaginitis in rats","authors":"O. Dolzhykova, R. Yeromenko","doi":"10.15587/2519-8025.2022.271039","DOIUrl":"https://doi.org/10.15587/2519-8025.2022.271039","url":null,"abstract":"The aim. to study the therapeutic influence of new pessaries Melanizol based on metronidazole and tea tree oil on the background of model of “chemical” vaginitis in female rats. \u0000Materials and methods. The therapeutic effect of pessaries Melanizol was studied in female rats on the model of \"chemical\" vaginitis with using argentum nitrate, which provokes vaginitis not burdened by infection. Against the background of vaginitis, the general condition of animals, the dynamics of body weight, the morphological composition of peripheral blood and the erythrocyte sedimentation rate (ESR), pH, biochemical parameters (total protein, ALT and glucose), temperature in the vagina of rats, macroscopically assessed changes in the vaginal mucosa (VM) were studied. \u0000Results and discussion. Against the background of pathology in the animals, a shift in the studied indicators was observed. Pessaries Melanizol at the dose of 21 mg/kg make a therapeutic effect on the model of experimental \"chemical\" vaginitis, caused by argentum nitrate, as indicated by the restoration of the VM state, reduced the number of leukocytes, restored the percentage composition of the leukocyte formula, reduced the amount of total protein and the cytolytic enzyme ALT in blood serum and vaginal contents, which indicates the anti-inflammatory and cytoprotective effect of the studied agent. The recovery of vaginal temperature, pH and the amount of glucose in the vaginal contents was also observed, which indicates the normalization of homeostasis in the vagina of experimental animals. According to these effects, the studied pessaries Melanizol were superior to the reference drug \"Gravagin\" at the dose of 30 mg/kg and were not inferior to the reference drug Hippophaes oleum pessaries (HOP) at the dose of 17.64 mg/kg. \u0000Conclusions. The therapeutic effect of new pessaries Melanizol based on metronidazole and tea tree oil on the model of \"chemical\" vaginitis in female rats has been proven. The investigated pessaries Melanizol are a promising drug for the treatment of non-specific vaginitis and require further research in this direction","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"116 12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84252977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}