Science China Life Sciences最新文献

{"title":"Unraveling the diversity of bile acid modifications by integrative mass spectrometry approaches.","authors":"Nan Xing, Yu-Fei Wang, Xue Ding, Hong-Li Tan, Yi-Fang Li, Guo-Xiang Sun, Rong-Rong He, Wan-Yang Sun","doi":"10.1007/s11427-025-2909-3","DOIUrl":"10.1007/s11427-025-2909-3","url":null,"abstract":"","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"2796-2799"},"PeriodicalIF":9.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144192077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional cancer-cell-nociceptive neuronal circuits drive gastric tumor progression.","authors":"Peng Gao, Jie Zhang, Yicheng Chen","doi":"10.1007/s11427-025-2964-5","DOIUrl":"10.1007/s11427-025-2964-5","url":null,"abstract":"","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"2825-2826"},"PeriodicalIF":9.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of multi-omics in aquaculture genetics and breeding: current status and future perspective.","authors":"Xiaofei Yu, Sara Faggion, Yuxiang Liu, Bo Wang, Qifan Zeng, Chunzhe Lu, Jingjie Hu, Luca Bargelloni, Lingzhao Fang, Zhenmin Bao","doi":"10.1007/s11427-024-2828-8","DOIUrl":"10.1007/s11427-024-2828-8","url":null,"abstract":"<p><p>Aquaculture, a fast-growing sector, plays an important role in the supply of nutrient-rich food for humans. Selective breeding is a promising approach to ensure the development and sustainability of intensive aquaculture systems by achieving cumulative and permanent improvements in desirable traits. The advancement of omics technologies offers unprecedented opportunities for genetic improvement, especially in the prioritization of SNPs to be used in the genomic selection and editing of economically important traits. This review highlights novel breeding strategies in aquaculture, emphasizing how multi-omics data can be integrated into selective breeding programs. Specifically, we discuss the current achievements in integrating functional data into conventional genomic prediction models and highlight the potential of artificial intelligence to efficiently map genes and predict phenotypes or genetic merit using multi-omics data. Ultimately, we discuss genome editing methods for their potential to fix existing alleles, introduce alleles from wild populations or related species, and create de novo alleles, with the general goal of improving commercially important traits in aquaculture species.</p>","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"2591-2604"},"PeriodicalIF":9.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144192164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pluripotent stem cell-derived cardiomyocyte transplantation: marching from bench to bedside.","authors":"Yi Hong, Jiarui Liu, Weixuan Wang, Hao Li, Weijing Kong, Xiaoxia Li, Wei Zhang, Sara Pahlavan, Yi-da Tang, Xi Wang, Kai Wang","doi":"10.1007/s11427-024-2801-x","DOIUrl":"10.1007/s11427-024-2801-x","url":null,"abstract":"<p><p>Cardiovascular diseases such as myocardial infarction, heart failure, and cardiomyopathy, persist as a leading global cause of death. Current treatment options have inherent limitations, particularly in terms of cardiac regeneration due to the limited regenerative capacity of adult human hearts. The transplantation of pluripotent stem cell-derived cardiomyocytes (PSC-CMs) has emerged as a promising and potential solution to address this challenge. This review aims to summarize the latest advancements and prospects of PSC-CM transplantation (PCT), along with the existing constraints, such as immune rejection and engraftment arrhythmias, and corresponding solutions. Encompassing a comprehensive range from fundamental research findings and preclinical experiments to ongoing clinical trials, we hope to offer insights into PCT from bench to bedside.</p>","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"2568-2590"},"PeriodicalIF":9.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Donor macrophage pyroptosis contributes to the development of aGVHD.","authors":"Xueyan Sun, Qingyuan Qu, Qi Chen, Fengqi Liu, Haixia Fu, Yuxiu Chen, Menglin Li, Yun He, Xiaolu Zhu, Xiangyu Zhao, Yu Wang, Xiaojun Huang, Xiaohui Zhang","doi":"10.1007/s11427-024-2908-8","DOIUrl":"10.1007/s11427-024-2908-8","url":null,"abstract":"<p><p>Pyroptosis, a novel form of inflammation-related programmed cell death that often occurs in myeloid cells, plays an important role in multiple inflammatory diseases. Our previous study revealed macrophage dysfunction in acute graft-versus-host disease (aGVHD). However, whether macrophages undergo pyroptosis in aGVHD remains unknown. In our study, macrophage pyroptosis was observed in aGVHD mice. Additionally, serum IL-1β and IL-18 levels were increased in aGVHD patients. Almost all peritoneal macrophages in the aGVHD mice were derived from the donors. Less pathological damage and a longer survival time were observed in the mice that received purified T cells and bone marrow (BM) from AAV9-F4/80-GSDMD mice, indicating that macrophage pyroptosis in donor mice promoted the development of aGVHD. In addition, decreased proportions of CD69⁺CD4⁺ T cells, Th1 cells and Th17 cells and an increased proportion of Tregs were observed in the recipients when GSDMD was suppressed in the donor macrophages. Administration of a pyroptosis inhibitor significantly alleviated the severity of aGVHD without impairing graft-versus-lymphoma (GVL) effects. Our results suggested that donor-derived macrophages undergo pyroptosis in aGVHD, and these cells might participate in the development of aGVHD by affecting the activation and differentiation of CD4⁺ cells. The pyroptosis inhibitor disulfiram is a potentially promising agent for aGVHD treatment.</p>","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"2605-2616"},"PeriodicalIF":9.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into tissue- and cell type-specific effects of Grb10 on pig skeletal muscle growth by multi-omics analysis.","authors":"Meng Li, Huan Liu, Tingting Lu, Suchen Li, Xinchen Zhou, Hui Miao, Yuelang Zhang, Chen Peng, Kaiyu Chen, Jiayi He, Yulong Yin, Pengju Zhao, Xinyan Han","doi":"10.1007/s11427-024-2910-7","DOIUrl":"10.1007/s11427-024-2910-7","url":null,"abstract":"<p><p>Domestic pigs are shaped by artificial and natural selection into obese and lean types that are closely related to muscle tissue. However, the key genes and regulatory mechanisms behind these developments remain largely unknown. Here, we pinpoint GRB10 specificity in muscle tissue and cells between obese and lean pigs by combining genomics, transcriptomics, epigenomics, and single-cell transcriptomics. GRB10 shows notable differences in divergent selection on haplotype blocks and expression levels between obese and lean pig breeds, with its expression profiles varying significantly by tissue and development stage. Notably, we identify a muscle-specific promoter of GRB10 and its transcription factor KLF15. This TF-promoter binding is verified by dual luciferase and chromatin immunoprecipitation (ChIP) assays, and is suggested to be conserved in humans. Single-nucleus RNA sequencing further highlights differential expression patterns of GRB10 between obese and lean pig breeds across various cell types. Type IIa myonuclei and TTN⁺ FAPs, which are more predominant in lean pigs, play a crucial role in myofibril assembly and muscle tissue development. These findings offer insights into the regulatory mechanisms controlling muscle growth. They highlight the tissue- and cell type-specific effects of GRB10 on muscle heterogeneity, which has potential applications in livestock breeding and human obesity research.</p>","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"2659-2675"},"PeriodicalIF":9.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR/Cas9-mediated promoterless gene targeting reduces lysosome storage in MPS VII mice.","authors":"Linlin Shan, Qiongyan He, Weifeng Zhang, Xiaojing Zheng, Junli Zhao, Peiyan Yang, Qinwen Mao, Haibin Xia","doi":"10.1007/s11427-024-2915-x","DOIUrl":"10.1007/s11427-024-2915-x","url":null,"abstract":"<p><p>Targeted gene integration mediated by CRISPR/Cas9 is a promising therapeutic strategy for monogenic autosomal recessive diseases. In this study, we established a novel all-in-one high-capacity adenovirus (HCAd) that can pack both CRISPR/Cas9 and donor DNA into the same vector and tested it on a mouse model of mucopolysaccharidosis type VII (MPS VII) caused by mutations in the β-glucuronidase (GUSB) gene. This system allowed targeted integration of promoterless GUSB in the mouse beta-actin gene (mActb) locus and the co-expression of GUSB with the self-cleaving peptide T2A (T2A) controlled by a strong endogenous mActb promoter. The in vivo results indicated that the serum GUSB level of MPS VII mice treated with a single intraperitoneal injection of the HCAd vector achieved 14% of that of wild-type mice, resulting in significant amelioration of lysosomal storage in the liver and spleen. Furthermore, the HCAd was injected intraventricularly in the brain of newborn MPS VII mice, leading to strongly positive GUSB enzyme staining in the choroid plexus and perivascular spaces of the periventricular regions and reduced lysosome storage. In summary, by using an all-in-one vector, the study provides a universal, one-for-all therapeutic for MPSVII, a disease caused by different mutations of the GUSB gene.</p>","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"2697-2706"},"PeriodicalIF":9.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Planer-polarized junction association of the RacGEF ELMO-Sponge contributes to planar polarity and germband extension in Drosophila embryos.","authors":"Deqing Kong, Jing He, Wangfang Liu, Zhaoxuan Zhang, Joerg Grosshans, Zhiyi Lv","doi":"10.1007/s11427-024-2778-4","DOIUrl":"10.1007/s11427-024-2778-4","url":null,"abstract":"<p><p>Planar polarization of epithelia is critical for tissue morphogenesis and function. Polarized cell behaviour requires the asymmetric distribution of key components. The planar polarized enrichment of F-actin and MyoII is required for Drosophila embryonic axis extension. The actomyosin enriches the cell junctions in the dorsoventral (vertical) versus anterior-posterior (horizontal) direction to shrink the vertical junctions and drive cell rearrangement. Rac GEF plays an essential role in the reorganization of the actin cytoskeleton in many cellular processes. However, whether and how Rac GEF is involved in the planar polarity of the cytoskeleton in Drosophila embryonic axis extension remains largely unclear. Here, we report that the planar polarized RacGEF ELMO-Sponge is essential for germband extension. We find that Sponge and ELMO form a complex and enrich the vertical rather than the horizontal junctions in germband cells. Critically, this planar polarized distribution is confined to the region of adherens junctions in the apical-basal dimension, where F-actin becomes concentrated. Both the amount and planar polarity of F-actin, MyoII, and E-cadherin were affected by ELMO knockdown. Sponge knockdown damaged tissue integrity during germband extension. Consistent with reduced MyoII levels, we observed reduced recoil after junction ablation and incomplete germband extension in sponge- or ELMO-depleted embryos. Taken together, our data suggest that the ELMO/Sponge complex is required for the planar polarized localization of F-actin and maintains junctional contractility, thereby promoting effective tissue elongation.</p>","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"2648-2658"},"PeriodicalIF":9.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144668274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress and gaps in antimicrobial resistance research within One Health sectors in China: a systematic analysis.","authors":"Cai Chen, Shu-Le Li, Jue Liu, Sabiha Y Essack, Yi Luo, Mui-Choo Jong, Xiang-Yu Dai, Yao-Yang Xu, Lise Korsten, David W Graham, Yong-Guan Zhu","doi":"10.1007/s11427-025-2955-6","DOIUrl":"10.1007/s11427-025-2955-6","url":null,"abstract":"<p><p>Antimicrobial resistance (AMR) presents a multifaceted health threat to humans, animals, plants, food systems, and environments. In response, China initiated extensive research to understand and address AMR. However, there has been a lack of analysis and synthesis of research results at the national level. This study establishes a national AMR knowledge repository through the systematic analysis of over 44,000 scientific publications (2000-2024), employing a machine learning framework that combines transformer-based language models and cluster analysis. Natural language processing (NLP) was used to identify key AMR research topics, subtopics, and AMR detection methods across One Health sectors, including changes over time. Main findings include: (i) China's AMR research in human health aligns with societal disease burdens, yet gaps exist for pathogens like Clostridium difficile and Hepatitis B virus, despite their significant risks in China. (ii) While AMR research in probiotics is increasing, potential risks of AMR transmission associated with their use are often underestimated, particularly regarding the post-marketing surveillance and standardization of probiotic products. (iii) Discovery of new antimicrobial agents and alternative therapies is crucial for AMR prevention in China. (iv) Artificial intelligence (AI) methods are promising to guide and accelerate research, including exploration of natural products and plant extracts. Overall, while the AMR research in China aligns with One Health principles, with the plant health sector surpassing global counterparts, food systems require enhanced efforts and cross-sectoral research, particularly in the development of effective AMR detection and surveillance technologies. This work demonstrates a replicable methodological framework for establishing and sustaining country-specific scientific evidence platforms, offering valuable data-driven support for synthesizing findings, decision-making, and developing current and future action plans to manage AMR from a One Health perspective.</p>","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"2755-2767"},"PeriodicalIF":9.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144668275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to: Local administration of liposomal-based Plekhf1 gene therapy attenuates pulmonary fibrosis by modulating macrophage polarization.","authors":"Lifeng Yan, Chenchen Hou, Juan Liu, Yi Wang, Chenxi Zeng, Jun Yu, Tianyu Zhou, Qing Zhou, Shengzhong Duan, Weining Xiong","doi":"10.1007/s11427-024-2931-2","DOIUrl":"10.1007/s11427-024-2931-2","url":null,"abstract":"","PeriodicalId":21576,"journal":{"name":"Science China Life Sciences","volume":" ","pages":"2827-2828"},"PeriodicalIF":9.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}