{"title":"The plasminogen-activating system in gingival fluid from adults. An intra-individual study before and after treatment of gingivitis.","authors":"B Kinnby, L Matsson, I Lecander","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>High concentrations of tissue plasminogen activator (t-PA) and placental type plasminogen activator inhibitor (PAI-2) have previously been found in gingival crevicular fluid (GCF) of adults and children. In the present study, intra-individual comparisons were made of the concentrations of t-PA, urokinase type plasminogen activator (u-PA), PAI-1, and PAI-2 in GCF from the same sites before and after periodontal treatment in eight healthy male volunteers aged 35-46 yr. The gingival state was assessed by exudate measurement, bleeding on standardized probing, and the gingival index of Löe & Silness 3 days before the start of the trial and on the day after completing a 21-day preventive program consisting of instruction and professional cleaning once a week. Eight sites per subject were selected for enzyme analyses, all showing improvement in gingival state during the period. Sampling of GCF at the start and at the end of the trial was done with small disks of Millipore-filter. t-PA and PAI-2 were analyzed with enzyme-linked immunosorbent assays with low method errors. The mean concentrations of t-PA were 0.73 mg/l before treatment and 0.49 mg/l after treatment. The mean concentrations of u-PA were 84.4 micrograms/l before treatment and 101.6 micrograms/l after treatment. PAI-1 was found in three subjects at the detection level. The mean PAI-2 concentrations were 2.19 mg/l before and 1.13 mg/l after treatment. The mean molar ratio PAs/PAI-2 was 0.47 before and 0.48 after treatment. This insignificant change implies a maintained proteolytic balance and indicates that PAI-2 is an important inhibitor of tissue proteolysis.</p>","PeriodicalId":21511,"journal":{"name":"Scandinavian journal of dental research","volume":"102 6","pages":"334-41"},"PeriodicalIF":0.0,"publicationDate":"1994-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18870871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Parathyroid hormone and enamel formation in rat maxillary incisors.","authors":"L Ranggård, J G Norén, C Engström","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Parathyroid hormone (PTH) plays an important role in regulating calcium in serum. It is also known to affect bone and dentin formation. The purpose of this investigation was to evaluate enamel formation in normal rats receiving added PTH. It is in two parts: a pilot study where a known method was tested, followed by the main study where the rats were given different doses of PTH. The enamel was examined in both studies and in the main study the ameloblasts were also investigated. Contradictory results were seen. In the pilot study, severe enamel aberrations occurred, while no divergence from normal amelogenesis was noted in the main study. A factor causing the disparate results was the use of a hard tissue marker (oxytetracycline) in the pilot study. It can be concluded that injections of PTH in doses that affect bone and dentin did not cause any changes in normal enamel formation.</p>","PeriodicalId":21511,"journal":{"name":"Scandinavian journal of dental research","volume":"99 2","pages":"90-5"},"PeriodicalIF":0.0,"publicationDate":"1991-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13209197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Microbial aspects of frequent intake of products with high sugar concentrations.","authors":"J Carlsson","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The Vipeholm Study showed that a frequent intake of products with high sugar concentrations increases caries activity. This effect of sugar was explained by the acid production of bacteria on the teeth. In the following 40 yr many microbial characteristics have been described, which are important in the pathogenesis of dental caries. This contribution focuses on one characteristic of the cariogenic bacteria; their acidurance. Compared to other oral bacteria the cariogenic lactobacilli and mutans streptococci have enzymes with greater resistance to acid, and greater capacities of the cells to extrude protons from the cytoplasm. The most important enzyme for the extrusion of protons is a membrane-bound proton-translocating ATPase. This enzyme is found in higher amounts and is working at lower pH in the cariogenic bacteria. Another important characteristic of the cariogenic bacteria is their production of lactic acid when they are exposed to high concentrations of sugar. Lactic acid has a pK of 3.8, while other carboxylic acids have a pK of 4.8. The bacterial cell membrane is permeable to undissociated carboxylic acids. These undissociated acids act as carriers to bring protons back into the cytoplasm. Bacteria with lactic acid as fermentation product have therefore the possibility to grow and produce acids at one pH unit lower than bacteria with, for example, acetic, propionic, or butyric acid as fermentation product.</p>","PeriodicalId":21511,"journal":{"name":"Scandinavian journal of dental research","volume":"97 2","pages":"110-4"},"PeriodicalIF":0.0,"publicationDate":"1989-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13791322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Chromatographic separation of alkaline phosphatase from dental enamel.","authors":"D Moe, S Kirkeby, E Salling","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Alkaline phosphatase (AP) was prepared from partly mineralized bovine enamel by extraction in phosphate buffer, centrifugation and various chromatographic techniques. Chromatofocusing showed that the enamel enzyme possessed five isoelectric points at the acid pH level ranging from pH 5.7 to pH 4.4. Three enzyme peaks were eluted using low pressure chromatography with a Bio-gel column. With a HPLC gel filtration column the separation of the enamel extract resulted in only one peak with AP activity. The fractions of this peak were used to produce an antibody against bovine AP.</p>","PeriodicalId":21511,"journal":{"name":"Scandinavian journal of dental research","volume":"97 1","pages":"8-13"},"PeriodicalIF":0.0,"publicationDate":"1989-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13850970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Formation and early prenatal location of the human mental foramen.","authors":"I Kjaer","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The formation and early prenatal location of the human mental foramen was investigated on 43 human fetuses. Histochemical methods supplemented by macroscopic visualization were used. Similar studies on the mental foramen were not available in the literature. The study demonstrated constancy in the developmental sequence of the bony structures in the region of the mental foramen. The formation of the mental foramen was described and related to general developmental parameters such as CRL and CNO values (skeletal maturity indexes of the hand and foot). The study indicated that the very early position of the mental foramen was in the region of the interstitial bone between the primary canine and the primary first molar. A positional change in the dorsal direction was described during the first half of the prenatal period.</p>","PeriodicalId":21511,"journal":{"name":"Scandinavian journal of dental research","volume":"97 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"1989-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13851047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of the antineoplastic agents doxorubicin and methotrexate on rat caries and secondary dentin formation.","authors":"U Pajari","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Because childhood cancer and/or its therapy is known to increase both dental caries and developmental defects in enamel, this experimental study was carried out to obtain information on the effect of two frequently used anticancer drugs, doxorubicin and methotrexate, on fissure caries and dentinal metabolism in healthy rats. Both drugs significantly reduced caries prevalence in the rat molars, the dentinal lesions that developed also being smaller than in the controls. Secondary dentin formation was slightly reduced, probably because of the lower caries prevalence in the rat molars and/or because of the action of the drugs. Thus, these two drugs could scarcely have been the reason for the increased caries activity in cancer patients.</p>","PeriodicalId":21511,"journal":{"name":"Scandinavian journal of dental research","volume":"97 1","pages":"14-9"},"PeriodicalIF":0.0,"publicationDate":"1989-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13850964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Long-term retention of TiF4 and SnF2 after topical application to dentin in dogs.","authors":"A B Tveit, B Klinge, B Tötdal, K A Selvig","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>As part of an evaluation of the potential of topical application of TiF4 in the prevention or arrest of root caries, the longevity of the titanium-rich glaze and the long-term retention of titanium and fluoride in exposed dentin surfaces were examined in dogs. For comparison, the effect of fluoride in combination with another cation, tin, was studied as well. Facets of exposed dentin were prepared in two beagles and treated for 1 or 4 min with either a TiF4 or an SnF2 solution (1.1 M F, native pH). After 3, 7, or 21 wk, the experimental surfaces were analyzed for fluoride, titanium, tin, and calcium using an electron microprobe. Dentin surfaces treated with TiF4 for 1 min showed fluoride concentrations ranging from less than 0.15% to 1.03%, while those treated for 4 min with SnF2 showed values from 0.22% to 1.28%. The calcium analyses revealed partial surface demineralization in one half of the SnF2-treated specimens, while this was a rare finding in the TiF4 group. The findings show that a brief application of TiF4 to exposed dentin results in approximately the same increase in fluoride content as a 4-min application of SnF2, with, however, less surface demineralization. In addition, the presence of a surface glaze may impart an added benefit to the use of TiF4 as a topical agent in the prevention of root caries.</p>","PeriodicalId":21511,"journal":{"name":"Scandinavian journal of dental research","volume":"96 6","pages":"536-40"},"PeriodicalIF":0.0,"publicationDate":"1988-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14336614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A 10-year follow-up study of the masticatory system after treatment with osseointegrated implant bridges.","authors":"T Haraldson, G Zarb","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Twenty-one patients with osseointegrated implant bridges (OIB), with an average age of 65 yr, were functionally examined at a 10-yr follow-up. Altogether 27 OIBs, supported by 138 fixtures, were examined. The examination comprised an anamnestic and clinical examination and registration of bite force. 90% of the patients were very satisfied with the outcome of the treatment. According to the anamnestic dysfunction index (Ai), 80% of the patients were symptom free in their masticatory system. The occlusal state was found to be good. Seventy-five percent of the patients were found to have only mild dysfunction of their masticatory system or to be symptomless according to the clinical dysfunction index (Di). The bite force ranged from 3 to 118 N at gentle biting, from 25 to 250 N at \"biting as when chewing\" and from 77.5 to 495.5 N at maximal bite force. On average the bite force had improved significantly at all bite-force levels tested compared to an examination 10 yr earlier. It is concluded that oral function in patients with OIBs is very favorable.</p>","PeriodicalId":21511,"journal":{"name":"Scandinavian journal of dental research","volume":"96 3","pages":"243-52"},"PeriodicalIF":0.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14294625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Reliability of the periapical index scoring system.","authors":"D Orstavik","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Data accumulated in one methodological and two longitudinal clinical studies employing the periapical index (PAI) scoring system were analyzed to assess the usefulness and reliability of the PAI system. More than 93% of pairs of PAI scores were either identical or deviated from each other with one step only for intra- and interexaminer agreement in scoring the same radiograph as well as for intraexaminer agreement in scoring two radiographs of the same root. Untreated roots which on completion of the longitudinal studies were judged to have healthy periapices, were scored as diseased less than 3% of the time during the study. Diseased teeth were scored as healthy less than 7% of the time. The results suggest that the PAI scoring system may be used with a high degree of reliability.</p>","PeriodicalId":21511,"journal":{"name":"Scandinavian journal of dental research","volume":"96 2","pages":"108-11"},"PeriodicalIF":0.0,"publicationDate":"1988-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14292411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biophysical and microbiologic studies of \"heavy\" and \"light\" plaque formers.","authors":"T Simonsson, S Edwardsson, P O Glantz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In order to trace factors possibly influencing initial bacterial colonization of tooth surfaces, a multiple linear regression analysis was performed on the present results as well as on those from certain clinical and biochemical studies previously performed in the same subjects. No statistically significant differences were observed between the two groups of plaque formers for the individual parameters. The multiple regression analysis showed, however, that the calculated work of adhesion for two polar and one non polar test liquid, the rate of unstimulated salivary secretion, morphologic differences in the dento-gingival areas of maxillary premolars and saliva-induced aggregation of S. mitior were crucial for about 90% of the variation in the number of CFU colonizing tooth surfaces after both 2 and 6 h. Both electrostatic and van der Waal's forces, as well as salivary flow conditions could thus have influenced the initial attachment of bacteria to the test surfaces. It is concluded that saliva including its suspended microorganisms could be looked upon as being a colloidal biological system, and if so, the initial bacterial colonization of tooth surfaces should follow known, general biophysical laws.</p>","PeriodicalId":21511,"journal":{"name":"Scandinavian journal of dental research","volume":"95 1","pages":"43-8"},"PeriodicalIF":0.0,"publicationDate":"1987-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14594652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}