Russian Journal of Biotherapy最新文献

{"title":"Preparation and investigation of properties of resveratrol and solubilizers compositions","authors":"S. Krechetov, M. S. Maslennikova, A. Kuksin, A. Malinkin, N. Solovieva, I. Krasnyuk","doi":"10.17650/1726-9784-2023-22-2-65-73","DOIUrl":"https://doi.org/10.17650/1726-9784-2023-22-2-65-73","url":null,"abstract":"Background. The search for technology or the use of excipients for the development of drugs containing poorly soluble pharmaceutical substances is an urgent task of pharmacy. According to the biopharmaceutical classification system, resveratrol belongs to group 4: the substance has low solubility and low permeability. Therefore, one of the priorities in the development of drugs with resveratrol is to increase its solubility and, as a result, increase its permeability. The article presents the results of physicochemical studies of resveratrol compositions with solubilizers (poloxamer 407, polysorbate 80, methyl-beta-cyclodextrin) after dissolution in a common solvent followed by evaporation.Aim. Obtaining compositions of resveratrol with solubilizers and auxiliary formations with an exclusive study of their properties for the development of solid dosage forms.Materials and methods. Trans-resveratrol, poloxamer 407, polysorbate 80, methyl-beta-cyclodextrin, citric and ascorbic acids, glycerin, methanol; high performance liquid chromatography, differential scanning calorimetry.Results. Physical and chemical studies of the obtained compositions of resveratrol with excipients were carried out. Excipients used: citric and ascorbic acids for all compositions, as well as glycerin for compositions with tween 80. Stability studies were carried out under conditions of «accelerated aging», as a result of which a noticeable decrease in the amount of resveratrol was found (80 % of the original). Studies of the stability of resveratrol have shown that at low pH, inherent in the stomach, its content remains practically unchanged for two days. And at pH close to neutral, the content of resveratrol is reduced to 90 % of the original amount. The obtained results indicate the feasibility of developing drugs that promote the absorption of resveratrol in the stomach and are stored at low temperatures (in the refrigerator).Conclusion. Compositions of resveratrol with solubilizers were obtained and the choice of excipients for the development of solid dosage forms was substantiated. Recommendations are given for obtaining solid dosage forms for each of the developed formulations and storage conditions are determined.","PeriodicalId":21505,"journal":{"name":"Russian Journal of Biotherapy","volume":"56 4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88406501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship of SLCO1B1 and ABCB1 gene polymorphisms with clinical variants of methotrexate toxicity in pediatric acute lymphoblastic leukemia therapy","authors":"О. D. Gurieva, М. I. Savelyeva, Z. Sozaeva, Т. T. Valiev","doi":"10.17650/1726-9784-2023-22-2-53-59","DOIUrl":"https://doi.org/10.17650/1726-9784-2023-22-2-53-59","url":null,"abstract":"Background. Despite the significant clinical efficacy of current treatment protocols for acute lymphoblastic leukemia (ALL) in children, high-dose methotrexate demonstrates significant interindividual variability in drug toxicity and disease outcomes due to polymorphisms of drug transporter genes and genes responsible for cytostatic metabolism, which makes pharmacogenetic studies increasingly relevant.Aim. To evaluate the association of ABCB1 (C3435T rs1045642, rs1128503, rs2032582, rs4148738), SLCO1B1 T521C rs4149056 gene polymorphisms with the main types of methotrexate toxicity and the onset of clinical events (death, recurrence, progression) during the treatment of childhood ALL.Materials and methods. The study enrolled 103 patients diagnosed with ALL who received therapy according to BFM group protocols (2002/2009), using high-dose (2000 and 5000 mg/m2) methotrexate. Laboratory methods using NCI toxicity scales (CTCAE v5.0 2018) were used to assess adverse reactions. Real-time polymerase chain reaction method was used to study ABCB1 and SLCO1B1 gene polymorphisms. The study material was peripheral blood. Material was sampled once, regardless of the duration of methotrexate therapy. SPSS Statistics 21.0 software was used for statistical processing of the results. Analysis of associations was performed using the χ2 criterion and Fisher’s exact test.Results. Development of infectious complications, oropharyngeal mucositis, delayed MTX elimination, events were significantly associated with polymorphisms of the studied genes: SLCO1B1 T521C rs4149056, ABCB1 rs4148738, ABCB1 rs1128503, which correlates with the data of world scientific literature.Conclusion. Determination of polymorphisms of genes responsible for the transport and metabolism of methotrexate is a promising and dynamically developing area of clinical oncology.","PeriodicalId":21505,"journal":{"name":"Russian Journal of Biotherapy","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89522644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical significance of detection of micrometastases in lymph nodes in ovarian cancer","authors":"Yu. V. Aldushkina, N. Anisimova, A. N. Gritsai, K. Novruzov, Yu. I. Dolzhikova, O. A. Odaryuk, D. V. Filonenko","doi":"10.17650/1726-9784-2023-22-2-20-26","DOIUrl":"https://doi.org/10.17650/1726-9784-2023-22-2-20-26","url":null,"abstract":"Ovarian cancer of epithelial origin is the most common type of oncological process in this organ and is characterized by a high probability of fatal outcome. It is believed that this is due to insufficiently effective diagnosis of the prevalence and severity of this disease. This review presents data on the development of disease recurrence in a significant number of patients after cytoreductive surgery. According to some authors, the reason for the high risk of recurrence is the insufficient information content of the method of studying histological preparations of regional lymph nodes after staining with hematoxylin and eosin to detect small-sized metastases, including isolated tumor cells and micrometastases. To improve the accuracy of such diagnostics, it is recommended to supplement the study of multilevel sections of lymph nodes using routine staining with the use of immunohistochemistry and other methods using various antibodies to cytokeratins, along with other epithelial markers. The review provides an analysis of the opinions of various researchers on the prognostic significance of the detection of micrometastases in the lymph nodes, which can increase the effectiveness of therapy due to a more accurate assessment of the prevalence of the disease and correction of the tactics of treating patients with various oncological diseases.","PeriodicalId":21505,"journal":{"name":"Russian Journal of Biotherapy","volume":"90 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80398533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunological aspects of hematopoietic stem cell transplantation for hematologic malignancies and some immunological peculiarities of cancer radioimmunotherapy","authors":"A. Shirin, O. Baranova, V. V. Reshetnikova","doi":"10.17650/1726-9784-2023-22-2-10-19","DOIUrl":"https://doi.org/10.17650/1726-9784-2023-22-2-10-19","url":null,"abstract":"This literature review provides basic information about the role of hematopoietic stem cell transplantation in the treatment of myelodysplastic syndromes, acute myeloid leukemia, acute lymphoblastic leukemia. The recommendations for the use of stem cell transplantation are indicated. Conditioning regimens (myeloablative, non-myeloablative, reduced intensity), their advantages and disadvantages are considered. The issues of prevention and treatment of graft-versus-host disease, including graft preparation (T-cell depletion), extracorporeal photopheresis and mechanisms of graft-versus-tumor (-leukemia) reaction are highlighted. The historical milestones of theimmunotherapy development, the creation of therapeutic monoclonal antibodies, the development of targeted therapy, for example, conjugates of monoclonal antibodies with cytostatic drugs and radionuclides (targeted radionuclide therapy) are reported. Information about radioimmunotherapy as a method used for treatment of solid tumors and non-Hodgkin’s lymphoma, and information on the use of mesenchymal stem cells for the treatment and prevention of the graft-versus-host reaction are presented.","PeriodicalId":21505,"journal":{"name":"Russian Journal of Biotherapy","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85436223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"To the 100th anniversary of the academician Anatoliy Andreevich Vorobyov (05.02.1923–23.03.2006)","authors":"A. Editorial","doi":"10.17650/1726-9784-2023-22-1-82-83","DOIUrl":"https://doi.org/10.17650/1726-9784-2023-22-1-82-83","url":null,"abstract":"<jats:p>.</jats:p>","PeriodicalId":21505,"journal":{"name":"Russian Journal of Biotherapy","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75887902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmaceutical development for transdermal dosage forms of nifedipine: justification of the choice and specificity of the action","authors":"V. V. Tokaev, E. Stepanova, M. Makieva, Yu. A. Morozov, D. Pozdnyakov, D. I. Gagloeva","doi":"10.17650/1726-9784-2023-22-1-76-81","DOIUrl":"https://doi.org/10.17650/1726-9784-2023-22-1-76-81","url":null,"abstract":"Background. Present day in medicine can be assessed as an active period of the fight against cardiovascular diseases. This group of diseases cannot be considered obsolete in any way, and even taking into account the emerging new pathology – coronavirus infection – cardiovascular diseases have consolidated their dominant position. Among the drugs used for appropriate pharmacotherapy, stands out nifedipine (1,4-dihydro-2,6-dimethyl-4-(2-nitrophenyl)-3,5-pyridindicarboxylic acid dimethyl ether) – a drug that can inhibit the flow of calcium into the cardiomyocytes of vascular smooth muscle cells. The drug has been on the domestic pharmaceutical market for quite a long time, but it is still in demand. However, nifedipine has quite pronounced side effects, including those associated with its oral administration – nausea caused by irritation of the gastrointestinal tract, rarely gum hyperplasia. And at the same time, the main modern dosage form of nifedipine is tablets. Therefore, expanding the range of its dosage forms is an urgent issue. Moreover, well-chosen dosage forms reduce the side effect of the drug. This is especially true for extended versions. So pharmaceutical development in relation to the creation of original dosage forms of nifedipine is quite appropriate.Aim. Determination of the possibility of absorption of nifedipine from the proposed dosage forms – transdermal patch and transdermal gel.Materials and methods. To determine nifedipine in rat blood plasma samples, high-performance liquid chromatography was used (chromatograph “Millichrome A-02” (Institute of Chromatography “EcoNova”, LLC, Russia).Results. Chromatograms of blood plasma samples of all animals on whose skin a transdermal gel or patch was applied revealed a peak coinciding in retention time with the peak of the standard sample of nifedipine.Conclusion. The possibility of penetration of nifedipine from the patch and transdermal gel through intact animal skin has been confirmed.","PeriodicalId":21505,"journal":{"name":"Russian Journal of Biotherapy","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78916885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship of lymphoid populations (infiltration) of the primary tumor with bone marrow immune responses in patients with breast cancer","authors":"S. Chulkova, E. Sholokhova, I. Poddubnaya, I. S. Stylidi, A. Egorova, N. A. Kozlov, N. Tupitsyn","doi":"10.17650/1726-9784-2023-22-1-49-61","DOIUrl":"https://doi.org/10.17650/1726-9784-2023-22-1-49-61","url":null,"abstract":"Backgraund. Currently, immunotherapy is firmly established in the standard of cancer treatment. The basis for the appointment of immunotherapy are immunological tumor markers, which include lymphoid infiltration, a detailed study of which has received increasing attention in the last decade. An undoubted interest is the study of lymphoid infiltration, not only depending on the morpho-clinical parameters of breast cancer (BC), but also on the immune system of the bone marrow.Aim. To evaluate the infiltration of the primary tumor by lymphocytes depending on the morpho-clinical characteristics of BC and immune responses in the bone marrow.Materials and methods. This study included 125 patients with BC who received treatment at the “N.N. Blokhin National Medical Research Center of Oncology” of the Ministry of Health of Russia. Tumor stage II was prevailed, а moderate degree of differentiation (G2) was more often noted. The luminal BC – 67 %, non-luminal – 33 %. Immunophenotyping of the primary tumor: cryostat sections, ZEISS Axioscope luminescent microscope (Zeiss AG, Germany). CD45+, CD38+, T- and B-cell infiltration were assessed. Bone marrow: CD3+, CD4+, CD8+, CD19+, CD16+, CD56+ lymphocytes and their subpopulations were studied (FACSCanto II flow cytometer, Kaluza Analysis v2.1 program (Beckman Coulter, USA)).Results. CD45+ infiltration was noted in 50.5 % of cases (severe in 30 %, moderate – 26.4 %). CD8+ cells significantly infiltrated the tumor in 21.4 % of cases. CD38+ infiltration was observed in 40 %. In the non-luminal BC, severe CD45 infiltration was observed more frequently than in the luminal (33 % vs 26 %). CD38+ infiltration is expressed in non-luminal BC (p = 0.016). CD45+ infiltration was positively correlated with earlier stages (p = 0.071) more pronounced in infiltrative ductal BC, than in lobular BC: 59.2 % vs 20 % (p = 0.05). The content of CD45RO+cells in bone marrow in the luminal BC is higher than in the non-luminal: 37.3 ± 2.3 % vs 28 ± 2.8 % (p = 0.04). The number of CD19+CD38+ cells, on the contrary, is less: 24.2 ± 2 % vs 34.8 ± 6 % (p = 0.041). Tumor-infiltrating lymphocytes highly correlated with bone marrow lymphoid populations: CD38+ cells with NK-bone marrow cells; CD4+ cells with the B-precursors; CD8+cells with the B1-lymphocytes.Conclusion. Lymphoid infiltration of BC is associated with stage, tumor size, histological type and biological subtype. Intratumoral populations CD38+, CD4+, CD3+, CD8+ cells are in a negative correlation with bone marrow lymphoid populations.","PeriodicalId":21505,"journal":{"name":"Russian Journal of Biotherapy","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89014992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Features of risk analysis, assessment and control methods in pharmaceutical development","authors":"S. Tishkov, E. V. Blynskaya, K. Alekseev, V. Alekseev","doi":"10.17650/1726-9784-2023-22-1-28-41","DOIUrl":"https://doi.org/10.17650/1726-9784-2023-22-1-28-41","url":null,"abstract":"Quality Risk Management (QRM) is an important aspect of pharmaceutical development in the Quality-by-Design paradigm, since it determines the Design Space, critical quality attributes and critical process attributes.The purpose of this review is to systematize and analyze the main aspects of the QRM system in relation to each element of pharmaceutical development. This review article discusses the following key elements of QRM such as: risk analysis, risk control, risk communication, risk verification. For risk analysis, the advantages and disadvantages of QRM tools such as Preliminary Hazard Analysis, Failure Mode Effects Analysis, Fault Tree Analysis are presented and analyzed, Hazard Operability Analysis and Hazard Analysis and Critical Control Points. In addition, a description of the methods of mathematical planning and design of experiments for pharmaceutical development and QRM is given and their significance for these systems is determined. Elements of QRM such as root cause analysis, brainstorming, and a corrective and preventive action procedure are given as risk control and mitigation tools.In this paper, the main stages of QRM are considered and conclusions are formulated regarding the main advantages and disadvantages of the methods of identification, analysis, control, risk reporting, and the main ways for further improvement of the presented system are proposed.","PeriodicalId":21505,"journal":{"name":"Russian Journal of Biotherapy","volume":"21 2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83769624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoprobes based on fluorescent semiconductor nanocrystals and single-domain antibodies for highly sensitive detection of epidermal growth factor receptor in tumor cells","authors":"G. Nifontova, D. Kalenichenko, M. Baryshnikova, Z. Sokolova, P. Samokhvalov, A. Karaulov, A. Sukhanova, I. Nabiev","doi":"10.17650/1726-9784-2023-22-1-68-75","DOIUrl":"https://doi.org/10.17650/1726-9784-2023-22-1-68-75","url":null,"abstract":"Background. The development of highly oriented conjugates of quantum dots (QDs) and single-domain antibodies (sdAbs) as innovative fluorescence imaging nanoprobes that specifically recognize tumor biomarkers, in particular, epidermal growth factor receptor (EGFR), is a promising approach to improving immunohistochemical tumor typing.Aim. The study was aimed at developing fluorescent nanoprobes based on QDs and sdAbs that specifically recognize EGFR, as well as evaluating their functional characteristics (size and optical properties) and functional activity.Materials and methods. QDs were obtained using high-temperature organometallic synthesis and transferred into the aqueous phase by means of stepwise replacement of ligands on the QD surface. The QDs and sdAbs were conjugated in an oriented manner using a bifunctional cross-linking agent. Detailed characteristics of the resulting conjugates were analyzed by the dynamic light scattering and immunoassay methods. Functional activity was assessed on the model human epidermoid carcinoma cells line A431.Results. The QD–sdAb conjugates have been standardized in terms of control parameters determining their functional activity, in particular, hydrodynamic diameter and efficiency of binding with target tumor cells. They are characterized by high dispersity, homogeneity, and specific functional activity towards their molecular target.Conclusion. The results demonstrate the potential use of the designed QD–sdAb conjugates for EGRF detection in immunohistochemical typing of tumor.","PeriodicalId":21505,"journal":{"name":"Russian Journal of Biotherapy","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83778493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anemia syndrome associated with malignant neoplasms in patients with colorectal cancer","authors":"V. N. Blindar, M. M. Dobrovolskaya, G. N. Zubrikhina, T. V. Davydova","doi":"10.17650/1726-9784-2023-22-1-62-67","DOIUrl":"https://doi.org/10.17650/1726-9784-2023-22-1-62-67","url":null,"abstract":"Background. Colorectal cancer is the most commonly diagnosed cancer among all malignant neoplasms. This disease is accompanied by the development of anemic syndrome (AS). This complication not only worsens the results of treatment and, as a result, reduces overall survival, but also reduces the quality of life of patients, reduces adherence to treatment.Aim. To study the expression of hepcidin 25 (HP25), prohepcidin (PROHP), ferritin (FR), interleukin 6 (IL-6) and metabolites of nitric oxide (NOx) in the peripheral blood of patients colorectal cancer, to assess their relationship with the clinical course of the disease and with AS.Materials and methods. The study was conducted in 41 patients with colorectal cancer and AS. The content of FR, PROHP, HP25, IL-6 was determined using enzyme immunoassay. The total content of NOx in blood serum was determined using the Griess reagent after the reduction of nitrate to nitrite with cadmium granules in the presence of zinc.Results. There are three types of AS. A characteristic feature of which was microcytosis (MCV – 74.3 ± 2.1 fl) and hypochromia (MHC – 22.9 ± 1.2 pg) of erythrocytes. Type 1 of AS – iron deficiency anemia (IDA), type 2 – anemia of chronic diseases (ACD) in combination with iron deficiency erythropoiesis and type 3 – ACD with functional iron deficiency (FID). True IDA was detected in 15 (36.6 %) patients, which was classically characterized by: low concentration of FR, PROGP, GP25, IL-6 and NOx. A less significant group (9 patients, 21.9 %) had a high concentration of FR and low PROHP, GP25, IL-6, NOx, which indicated iron deficiency against the background of ACD. It differed from the first group with IDA in a significant concentration of FR, which may indicate the transition of IDA to the chronic phase of AS. Third group (17 patients, 41.5 %) – with FID – turned out to be the most numerous and characterized by a high concentration of FR (386.7 ± 41.2 ng/ml), GP25 (43.2 ± 7.1 ng/ml), PROHP (283.3 ± 18.5 ng/ml), IL-6 (24.8 ± 5.5 pg/ml), NOx (39.7 ± 5.5 µmol/l), the values were significantly higher (p <0.001) than in patients with IDA and ACD with iron deficiency erythropoiesis.Conclusion. In patients with a widespread tumor process, AS with FID is most often detected. FID was accompanied by hyperproduction of FR, IL-6, GP25, PROGP and NOx. A close correlation was noted between the studied parameters with an increase in the T-stage of colorectal cancer. This may indicate the relationship of these proteins in the development of cytokine-induced anemia in cancer patients. The data obtained can be widely used to assess the state of metabolic disorders in anemia associated with malignant neoplasms for the differential diagnosis of AS variants and adequate treatment.","PeriodicalId":21505,"journal":{"name":"Russian Journal of Biotherapy","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84646367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}