Russian Journal of Biotherapy最新文献

{"title":"Cytopathogenic effect of Streptococcus pneumoniae native pneumolysin in CHO-K1 cells","authors":"E. A. Kurbatova, I. V. Yakovleva, N. F. Gavrilova, E. S. Petukhova, I. B. Semenova, A. E. Zaitsev, Y. Volokh, A. Leonova, A. Poddubikov","doi":"10.17650/1726-9784-2024-23-1-51-57","DOIUrl":"https://doi.org/10.17650/1726-9784-2024-23-1-51-57","url":null,"abstract":"","PeriodicalId":21505,"journal":{"name":"Russian Journal of Biotherapy","volume":" 41","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140386412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitory effect of lomustine on the growth of spontaneous HER2-positive mammary tumors in transgenic mice","authors":"V. A. Alexandrov, A. Stukov, Yuliya G. Zmitrichenko, O. Belyaeva, G. V. Tochilnikov","doi":"10.17650/1726-9784-2024-23-1-45-50","DOIUrl":"https://doi.org/10.17650/1726-9784-2024-23-1-45-50","url":null,"abstract":"","PeriodicalId":21505,"journal":{"name":"Russian Journal of Biotherapy","volume":" 39","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140386455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Test Dissolution for quality control of water-insoluble somatostatine analog","authors":"E. V. Ignatieva, M. N. Kraeva, I. V. Yartseva, D. V. Podymova, O. L. Orlova, D. V. Gusev, Y. A. Poskedova, T. M. Litvinova, Z. S. Sprakh","doi":"10.17650/1726-9784-2024-23-1-66-74","DOIUrl":"https://doi.org/10.17650/1726-9784-2024-23-1-66-74","url":null,"abstract":"","PeriodicalId":21505,"journal":{"name":"Russian Journal of Biotherapy","volume":" 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140386858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of solid dispersions with polyvinylpyrrolidone on the solubility of GML-3","authors":"V. Markeev, S. V. Tishkov, E. V. Blynskaya, K. V. Alekseev","doi":"10.17650/1726-9784-2024-23-1-58-65","DOIUrl":"https://doi.org/10.17650/1726-9784-2024-23-1-58-65","url":null,"abstract":"","PeriodicalId":21505,"journal":{"name":"Russian Journal of Biotherapy","volume":" 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140386316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"B16 melanoma growth characteristic in C57BL/6 mice with various methods of obtaining tumor material and syngeneic tumor transplantation sites","authors":"I. G. Murazov, Ia. V. Agatsarskaya, K. Kryshen","doi":"10.17650/1726-9784-2024-23-1-28-36","DOIUrl":"https://doi.org/10.17650/1726-9784-2024-23-1-28-36","url":null,"abstract":"","PeriodicalId":21505,"journal":{"name":"Russian Journal of Biotherapy","volume":" 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140387801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of inactivating heterozygous mutations in DNA repair genes on experimental lung carcinogenesis in mice","authors":"M. Maydin, M. Yurova, E. Fedoros, P. V. Sergiev, S. Aleksakhina, E. A. Otradnova, S. S. Kruglov, E. N. Imyanitov","doi":"10.17650/1726-9784-2024-23-1-37-44","DOIUrl":"https://doi.org/10.17650/1726-9784-2024-23-1-37-44","url":null,"abstract":"Background. Inactivating mutations in Chek2 and Gprc5a genes are known to be associated with cancer development. Experimental carcinogenesis studies in genetically modified mice generate new data on their influence on pathology development.Aim. In the present study in a model of lung carcinogenesis, survival parameters as well as tumor multiplicity and size in mice with Chek2 and Gprc5a heterozygous inactivating mutations were evaluated.Material and methods. F2 hybrid mice from crosses between CBAB6F1 males heterozygous for the studied mutations and wild-type BALB / c females were used: Chek2dAA-carriers (76 males and 64 females) and Gprc5ainsA-carriers (60 males and 42 females). Starting at four months of age, mice received urethane (ethyl carbamate) intraperitoneally at a dose of 600 mg / kg weekly for 6 weeks. After genotyping by allele-specific PCR, animals were allocated to groups. Carcinogenesis parameters were evaluated 40 weeks after the beginning of the experiment.Results. The proportion of mice with mutations surviving to the age of three months roughly followed the Mendelian distribution (35 / 41 males and 33 / 31 females) for the offspring of males heterozygous for Chek2dAA and was significantly lower in the case of Gprc5ainsA (20 / 40 males and 17 / 25 females, p = 0.043). The death of Gprc5ainsA carriers during the experiment was also higher than in the control group (p = 0.0506 in females). Synchronous lung and thymus neoplasms were found in 2 out of 4 Gprc5ainsA females that died before the end of the experiment, which were not found in other groups. At the end of the experiment, no significant differences in tumor multiplicity, mean linear size, and volume were found between the groups of mice with and without mutations.Conclusion. It was found that heterozygous inactivating mutation Chek2dAA does not affect early age development and does not modify the parameters of induced lung carcinogenesis in mice. Heterozygous carriage of Gprc5ainsA mutation in mice increases the risk of early death and sensitivity to the toxic and carcinogenic effects of urethane.","PeriodicalId":21505,"journal":{"name":"Russian Journal of Biotherapy","volume":" 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140387649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combretastatins A-4 and A-1 and their derivatives: Review","authors":"E. R. Nemtsova, N. Morozova, A. Plyutinskaya, A. Noev, A. Pankratov, P. Shegay","doi":"10.17650/1726-9784-2024-23-1-10-18","DOIUrl":"https://doi.org/10.17650/1726-9784-2024-23-1-10-18","url":null,"abstract":"","PeriodicalId":21505,"journal":{"name":"Russian Journal of Biotherapy","volume":" 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140387840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of circulating tumor DNA of B16-F10 melanoma syngenic model in C57BL6 mice plasma","authors":"K. M. Konoplina, A. A. Malchenkova, N. A. Kalinina, M. V. Panyugina, E. Kosobokova, V. Kosorukov","doi":"10.17650/1726-9784-2024-23-1-19-27","DOIUrl":"https://doi.org/10.17650/1726-9784-2024-23-1-19-27","url":null,"abstract":"","PeriodicalId":21505,"journal":{"name":"Russian Journal of Biotherapy","volume":" 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140387546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solubilization of 3-hydroxyquinazoline derivative with antitumor activity","authors":"E. Sanarova, A. Lantsova, L. Nikolaeva, V. Osipov, D. V. Gusev, L. Borisova","doi":"10.17650/1726-9784-2023-22-4-60-67","DOIUrl":"https://doi.org/10.17650/1726-9784-2023-22-4-60-67","url":null,"abstract":"Background. Increasing the solubility of new pharmacologically active substances is one of the main tasks of pharmacy. It becomes even more relevant in the field of creating dosage forms for difficult-to-solubilise substances with a new mechanism of action, as in the case of 3-hydroxyquinazoline derivatives, which have shown in vivo experiments the ability to activate tumour cell death by ferroptosis. This includes OVF-009 – an original domestic substance. Due to the solubility of this compound in oils, the technology of its solubilisation using modified castor oil (Kolliphor® ELP, BASF, Germany), approved for parenteral use, was proposed.Aim. To create a model dosage form for a new hydrophobic quinazoline derivative in order to further evaluate the spectrum of its antitumour activity in in vivo experiments.Materials and methods. OVF-009, Kolliphor® ELP, 95 % ethanol, Kollidon 17 PF (BASF, Germany), sodium hydroxide, phosphate buffer, water for injection; spontaneous micelle formation, ultrasound, potentiometry, dynamic light scattering, electrophoretic method, viscometry method, etc.Results. The main properties of micelle-forming solubiliser Kolliphor® ELP and its aqueous solutions were considered; 10 model solutions OVF-009 based only on Kolliphor® ELP and with addition of ethanol and Kollidon 17PF in different concentrations were obtained; the quality of the obtained compositions was evaluated by parameters – appearance, solution transparency, pH, stability over time and tolerability by laboratory animals. As a result, two formulations prepared on phosphate buffer were chosen: formulation No 5, which contains 10 % Kolliphor® ELP and additionally 10 % polyvinylpyrrolidone, and formulation No 8, in which in addition to the main solubiliser 10 % ethanol and 16 % polyvinylpyrrolidone were added. Both formulations have neutral pH, can be stored for 24 h and are tolerated by laboratory animals.Conclusion. The selected model compositions for solubilisation of the substance OVF-009 on the basis of Kolliphor® ELP allowed to provide the concentration of the active substance in the solution suitable for further biological experiments on animals.","PeriodicalId":21505,"journal":{"name":"Russian Journal of Biotherapy","volume":"46 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139244179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling the release of vinpocetine from microcapsules based on sodium alginate and chitosan by molecular dynamics","authors":"Y. Polkovnikova","doi":"10.17650/1726-9784-2023-22-4-68-75","DOIUrl":"https://doi.org/10.17650/1726-9784-2023-22-4-68-75","url":null,"abstract":"Introduction. when developing the composition of drugs, an actual direction is the use of computer modeling methods, including the methods of molecular dynamics (MD), which significantly expanded the possibilities of chemistry, providing spatial and temporal resolution that is inaccessible in experiments.Aim. To simulation of the release of vinpocetine from sodium alginate with a shell of chitosan into solvent media by the method of MD to determine the characteristics of computer simulation, which makes it possible to obtain microcapsules with desired biopharmaceutical properties. Materials and methods. To simulate the release of vinpocetine from sodium alginate with a shell of chitosan, the MD method in the GROMOS 54a7 force field was used using the Gromacs 2019 program. Using the HyperChem 8.0.1 program, the molecules of the components of the simulated systems were constructed. The models were parameterized using the Internet service Automated Topology Builder (http://atb.uq.edu.au/).Results. Based on the results of MD modeling, the van der waals interaction energies of vinpocetine with sodium alginate (alginic acid), with chitosan (chitosan-cation) and with a solvent in terms of 1 molecule of vinpocetine were calculated. The fractions of vinpocetine molecules not bound to the polymer were also calculated. It has been established that the average values of the energy of the van der waals interaction between vinpocetine and the solvent in an acidic medium are lower than in a neutral medium. Also, in an acidic environment, in contrast to a neutral environment, a slight release of vinpocetine is observed.Conclusion. In the course of the experiment, it was found that at pH 2.0, chitosan dissolves in an aqueous medium and a slight release of vinpocetine from alginic acid into an aqueous solution of chitosan is observed (the average proportion of vinpocetine molecules not associated with sodium alginate (alginic acid) and chitosan is 2.16 ± 2.33 %), the release of vinpocetine into water at pH 6.8 is not observed.","PeriodicalId":21505,"journal":{"name":"Russian Journal of Biotherapy","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139244558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}