Scanning microscopy最新文献_第8页

Cross-linking of cell surface receptors as a trigger or cell apoptosis and proliferation. 细胞表面受体的交联作为细胞凋亡和增殖的触发器。

Scanning microscopy Pub Date : 1995-09-01

Y N Korystov

引用次数: 0

Scanning electron microscopy and energy dispersive spectroscopy analysis of calciotraumatic lines in rat labial dentin after acute exposure to strontium chloride. 大鼠唇牙本质急性氯化锶暴露后钙损伤线的扫描电镜和能谱分析。

Scanning microscopy Pub Date : 1995-09-01

H Mishima, T Sakae, Y Kozawa

引用次数: 0

Atomic force microscopy of nucleoprotein complexes. 核蛋白复合物的原子力显微镜。

Scanning microscopy Pub Date : 1995-09-01

Y L Lyubchenko, B L Jacobs, S M Lindsay, A Stasiak

{"title":"Atomic force microscopy of nucleoprotein complexes.","authors":"Y L Lyubchenko, B L Jacobs, S M Lindsay, A Stasiak","doi":"","DOIUrl":"","url":null,"abstract":"Recent data on the AFM studies of nucleoprotein complexes of different types are reviewed in this paper. The first section describes the progress in the sample preparation methods for AFM studies of nucleic acids and nucleoprotein complexes. The second part of this paper reviews AFM data on studies of complexes of DNA with regulatory proteins. These studies include two different types of DNA distortion induced by proteins binding: local bending of DNA at sites of protein binding and formation of large loops due to protein-protein interactions between molecules bound to distant sites along the DNA molecules (DNA looping). The prospects for use of AFM for physical mapping of genomes are discussed in this section as well. The third part of the paper reviews data on studies of complexes of DNA with non-sequence specific binding proteins. Special emphasis is given to studies of chromatin which have resulted in progress in the understanding of structure of native chromatin fiber. In this section, novel data on AFM studies of RecA-DNA filaments and complexes of dsRNA with the dsRNA-specific protein p25 are also presented. Discussion of the substrate preparation procedures in relation to the AFM studies of nucleoprotein complexes is given in the final section.","PeriodicalId":21502,"journal":{"name":"Scanning microscopy","volume":"9 3","pages":"705-24; discussion 724-7"},"PeriodicalIF":0.0,"publicationDate":"1995-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18510735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of DNA fragmentation and color thresholding for objective quantitation of apoptotic cells. DNA片段化与颜色阈值法定量凋亡细胞的比较。

Scanning microscopy Pub Date : 1995-09-01

D R Plymale, D S Ng Tang, C D Fermin, D E Lewis, D S Martin, R F Garry

引用次数: 0

Improved methods for preserving macromolecular structures and visualizing them by fluorescence and scanning electron microscopy. 改进了保存大分子结构的方法，并通过荧光和扫描电子显微镜观察它们。

Scanning microscopy Pub Date : 1995-09-01

P B Bell, B Safiejko-Mroczka

{"title":"Improved methods for preserving macromolecular structures and visualizing them by fluorescence and scanning electron microscopy.","authors":"P B Bell, B Safiejko-Mroczka","doi":"","DOIUrl":"","url":null,"abstract":"To determine the optimal procedures to preserve cytoskeletal and other macromolecular structures for microscopic studies we have evaluated the effects of various methods to extract cultured cells. In this report, we compare results using different fixatives, crosslinking reagents, and permeabilization methods on (1) the labeling of cells for fluorescence microscopy with phalloidin or antibody against tubulin; and (2) the morphological preservation of macromolecular structures for scanning electron microscopy. Maximal labeling of F-actin with phalloidin was obtained by fixing cells in 4% paraformaldehyde (PFA) and labeling the unextracted cells with methanolic phalloidin, whereas maximal labeling of tubulin required prefixation with either PFA or the bifunctional protein crosslinking reagent, dithiobis (succinimidylpropionate) (DSP) and extraction with ethanol or Triton in a high salt buffer. However, for both qualitative and quantitative light and electron microscopic studies of intracellular macromolecular structures, prefixation with DSP and extracting with Triton X-100 in a stabilizing buffer is the overall method of choice for both labeling and morphological studies. Although other methods provide maximal labeling or preservation of specific structures, this method provides excellent preservation of morphological structure while allowing proteins to be preserved and labeled by specific probes.","PeriodicalId":21502,"journal":{"name":"Scanning microscopy","volume":"9 3","pages":"843-57; discussion 858-60"},"PeriodicalIF":0.0,"publicationDate":"1995-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18509259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Confocal laser scanning microscopic studies on alveolar bone remodeling with orthodontic tooth movement and retention. 正畸牙移动与固位对牙槽骨重塑的共聚焦激光扫描显微研究。

Scanning microscopy Pub Date : 1995-09-01

H Yagishita, S Iwatsubo, T Aoba

{"title":"Confocal laser scanning microscopic studies on alveolar bone remodeling with orthodontic tooth movement and retention.","authors":"H Yagishita, S Iwatsubo, T Aoba","doi":"","DOIUrl":"","url":null,"abstract":"Alveolar bone reconstruction in growing dog during the retention period following orthodontic tooth movement was studied. Three beagle dogs (8-10 kg body weight, about one-year-old) were used and two of the animals were subjected to histological observation. The upper 2nd and lower 3rd premolars on both sides were extracted prior to the orthodontic treatments. After a healing period of one month, the upper 3rd premolar and the lower 4th premolar on the right side were moved mesially with a conventional orthodontic force for 8 weeks, and then retained in their new position for 4 weeks. The contralateral corresponding premolars were used as control. The alveolar bone was double-labeled with tetracycline (TC) during the movement and calcein (Cal) during the retention period. Alveolar bone structure and labeling patterns were examined by contact microradiography, conventional fluorescence microscopy, and confocal laser scanning microscopy (CLSM). Optimizing the separation of TC and Cal labelings in the alveolar bone was attained by the simultaneous use of ultraviolet (364 nm) and argon (488 nm) laser sources for excitation of TC and Cal, respectively. Cal labeling, indicative of new bone deposition showed two distinct patterns: lamination at the periodontal surface and rings circumscribing the vascular canal. The cementum surface also exhibited active deposition during the experimental period. Bone formation was affected by slight changes in magnitude and direction of orthodontic or occlusal forces. CLSM is valuable in deciphering the process of alveolar bone remodeling.","PeriodicalId":21502,"journal":{"name":"Scanning microscopy","volume":"9 3","pages":"781-8"},"PeriodicalIF":0.0,"publicationDate":"1995-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18510008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scanning force microscopy of chromatin. 扫描力显微镜的染色质。

Scanning microscopy Pub Date : 1995-09-01

W Fritzsche, J Vesenka, E Henderson

引用次数: 0

Atomic Force microscopy of neuron networks. 神经元网络的原子力显微镜。

Scanning microscopy Pub Date : 1995-09-01

A Cricenti, G De Stasio, R Generosi, P Perfetti, M T Ciotti, D Mercanti

引用次数: 0

Consistency in calibrated backscattered electron images of calcified tissues and minerals analyzed in multiple imaging sessions. 在多次成像会议中分析钙化组织和矿物质的校准后向散射电子图像的一致性。

Scanning microscopy Pub Date : 1995-09-01

E G Vajda, J G Skedros, R D Bloebaum

{"title":"Consistency in calibrated backscattered electron images of calcified tissues and minerals analyzed in multiple imaging sessions.","authors":"E G Vajda, J G Skedros, R D Bloebaum","doi":"","DOIUrl":"","url":null,"abstract":"Pure metal standards have been used to calibrate the operating envionment in quatitative backscattered electron (BSE) imaging of mineralized tissue, allowing comparisons to be made between various mineralization states of bone at the microscopic level. It has not previuously been documented that calibration procedures produce consistent, reliable results over multiple imaging sessions. In this study, BSE images were obtained from bones, pure metals, and a naturally occurring mineral in multiple imaging sessions over a six day period. The graylevel histogram profile (GHP) from each specimen was analyzed for changes in the shape and relative placement on the graylevel spectrum. Computer controlled calibration and a restrospective calibration method using pure aluminum and pure magnesium-aluminum-zinc demonstrated consistency between imaging sessions. Calibrated weighted mean graylevels (WMGLs) for biological meterials had an average standard deviation of 5.9 graylevels (2.4% variation) during the course of the study. WMGLs for inorganic materials had an average standard deviation of 0.9 graylevels (0.4% variation). A trend towards increased image brightness, due to specimen and/or embedding media degradation, was observed in the biological tissues. No increase in rightness was observed for the inorgtanic specimens. Kurtosis and skewness tests revealed a slight deviation from normality in all specimens, which remained consistent between multiple imaging sessions. These results demonstrate the BSE image analysis of bones and mineral can be calibrated with negligible precision error allowing comparisons between data within and between multiple imaging sessions.","PeriodicalId":21502,"journal":{"name":"Scanning microscopy","volume":"9 3","pages":"741-53"},"PeriodicalIF":0.0,"publicationDate":"1995-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20488451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Escherichia coli on urine citrate and urease-induced crystallization. 大肠杆菌对尿柠檬酸盐和脲酶诱导结晶的影响。

Scanning microscopy Pub Date : 1995-09-01

A Edin-Liljegren, H H Hedelin, L Grenabo, S Pettersson

引用次数: 0