Russian Journal of Infection and Immunity最新文献

{"title":"INVESTIGATION OF BACTERIAL AND VIRAL SLOW INTERVERTEBRAL DISC INFECTIONS","authors":"S. Isroilov, Marsel Albertovich Shigapov, Artur Murasov, Aliya Gumerovna Kuzhabaeva, K. Nikonorov, Maria Vyacheslavovna Skudarnova","doi":"10.15789/2220-7619-iob-2052","DOIUrl":"https://doi.org/10.15789/2220-7619-iob-2052","url":null,"abstract":"Objective. Currently, few publications have been available regarding an effect of level, type of intrusion, and viral infections on spinal disc degeneration. The study was aimed to evaluate an incidence of indolent bacterial and viral intervertebral disc infections in unstratified group of patients operated on for spinal degenerative pathologies. \u0000Material and methods. For the study, intraoperative disc samples were collected from patients who underwent discectomy and total spinal fusion surgery for degenerative pathologies between the years December, 2018 to January, 2020. 447 samples were examined obtained from 392 patients (189 women and 203 men) with a mean age of 58.1 years. \u0000The following epidemiological and clinical data were collected: gender, age, spinal segments affected, type of surgical approach, preoperative C-reactive protein (CRP; reference range 0.00.5 mg/dL), and preoperative as well as 6-month postoperative ODI and NDI, both expressed as a percentage. The incidence of postoperative infections within six months after surgery as well as MI for each operated segment was assessed. Only patients with ventral cervical or dorsal thoracic access were enrolled in the study. In the lumbar region, the ventral assess was chosen for anterior lumbar interbody fusion, the lateral approach for oblique or extreme lateral interbody fusion, and the dorsal approach in case of standard microdiscectomy of posterior transforaminal interbody fusion or transforaminal interbody fusion. \u0000A logistic regression analysis was performed to model a risk of positive microbiological culture and a risk of emerging modal changes by using a set of independent variables selected in a stepwise manner by using the Akaike information criterion as a measure of quality of select procedure. \u0000Results. MRI for modal analysis was available for all 447 segments. MRI revealed 45 type 1 SCs (10.07% of all segments), 118 type 2 SCs (26.4%), and 5 type 3 SCs (1.12%). Microbiological culture was available in 410 segments. In these 410 segments, 39 type 1 MCs, 113 type 2 MCs, and 5 type 3 MCs were found. A statistically significant relationship was found between SC type 2 and a positive microbiological result (p = 0.0127). In contrast, there were no statistically significant associations for type 1 and type 3 SCs (p=0.3052 and p=0.0767, respectively). \u0000Conclusion. A relation between disc degeneration and infections seems unlikely. Histopathological evidence of inflammation in degenerated discs is rare and unrelated to microbiological findings.","PeriodicalId":21412,"journal":{"name":"Russian Journal of Infection and Immunity","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84660609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IgG-antibodies to individual cytomegalovirus (CMV) proteins and the peripheral blood subpopulation of lymphocytes in patients with anterior uveitis of varying severity","authors":"Galina Kriсhevskaya, N. Balatskaya, G. Alatortseva, E. Sorozhkina, Lyudmila Kovaleva, I. Kulikova, Vera V. Dotsenko, L. Nesterenko, L. Lukhverchik","doi":"10.15789/2220-7619-iat-8875","DOIUrl":"https://doi.org/10.15789/2220-7619-iat-8875","url":null,"abstract":"Relevance. Cytomegalovirus (CMV), a human beta-herpesvirus, persists in the human in a latent form for life after infection. CMV reactivation that occurs periodically can cause disease of end organs, including the eye, not only in immunosuppression, but also in immunocompetent people. Due to its immunosuppressive properties, persistent CMV is involved in the pathogenesis of diseases of various etiologies. Aim. To study the features of antibody formation to six individual CMV proteins and to conduct a correlation analysis with the indicators of the subpopulation of blood lymphocytes in patients with anterior uveitis of varying severity (without hypopion-mild course, with hypopion-more severe course). Materials and methods. 36 patients with anterior uveitis were examined. Group I consisted of 20 patients without hypopion, group II 16 people with hypopion. There were no characteristic features of CMV anterior uveitis(elevated intraocular pressure, stromal iris atrophy). IgG antibodies to CMV (markers of chronic infection) were determined in blood sera by ELISA, antibodies to individual recombinant CMV antigens were determined by Line-Immunoassay: to the main non-structural immediate early protein (IE), DNA-binding phosphoprotein p52, phosphoproteins of the tegument p150, p65, p28, GB-AD envelope glycoprotein. The subpopulation of lymphocytes was studied by flow cytometry: T-lymphocytes (CD3+), T-helper cells (CD3+CD4+CD8-), T-cytotoxic (CD3+CD4-CD8+), T-double-positive (CD3+CD4+CD8+), natural killers (CD16+CD56+), B-lymphocytes (CD19+). Statistical analysis was performed using StatTech v. 3.0.2 program (Stattech LLC, Russia). Results. In anterior uveitis of a light course, a statistically significant increase in the relative content of CD16+CD56+ and a decrease in the absolute number of CD3+CD8+ were found compared with anterior uveitis of a heavier course. Only in the group of patients with hypopion (more severe course) there was a direct correlation, a noticeable reliable relationship between the levels of antibodies to IE, p65, p28 antigens and the content of double-positive cells (CD3+CD4+CD8+) and an inverse reliable relationship between the levels of antibodies to IE, p65 and p52 antigens and CD19+ lymphocytes. Conclusion. The results obtained are consistent with the isolated reports on the role of double-positive lymphocytes in the pathogenesis of severe forms of some viral diseases. Further studies are needed to assess the effect of individual viral antigens on CD3+CD4+CD8+ content in patients with chronic CMV infection to confirm the role of double - positive lymphocytes in the pathogenesis of a more severe course of anterior uveitis.","PeriodicalId":21412,"journal":{"name":"Russian Journal of Infection and Immunity","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87233642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PROBIOTICS IN PREVENTION AND TREATMENT OF COVID-19: A SYSTEMATIC REVIEW OF CURRENT EVIDENCE","authors":"Seyedahmad Seyedalinaghi, Ramin Shahidi, Arian Afzalian, Parinaz Paranjkhoo, K. Ghorbanzadeh, Hengameh Mojdeganlou, A. Razi, Paniz Mojdeganlou, Mohsen Dashti, Sahar Nooralioghli Parikhani, Afsaneh Ghasemzadeh, Z. Pashaei, A. Karimi, E. Mehraeen, Daniel Hackett, Ahmadi Sepide","doi":"10.15789/2220-7619-pip-2118","DOIUrl":"https://doi.org/10.15789/2220-7619-pip-2118","url":null,"abstract":"Introduction: Clinical evidence suggests that certain probiotics may help treat and prevent viral infections. To date, the effectiveness of probiotics in the alleviation of COVID-19 has not been established. The aim of this systematic review was to assess the role of probiotics in the prevention and treatment of COVID-19. \u0000Methods: An extensive search of four electronic databases was performed which included Embase, Scopus, Web of Science, and PubMed from November 2019 to June 2022. After reviewing the references list of related articles additional studies were identified. A multiple combination of keywords validated by MESH were used to search the databases. Study selection was performed according to an inclusion and exclusion criteria. \u0000Results: Twenty-three articles met the study inclusion criteria. Six articles were conducted in vitro while the remaining studies were conducted in the human population (in vivo). The type of probiotic was defined in eighteen studies. There were two studies that used supplements (vitamins, herbals, minerals, etc.) in addition to probiotics. The largest sample size was 44,585 participants which were from a study that used an application-based survey. The majority of studies found that probiotics had a positive effect on the COVID-19 disease. The benefits included early remission of COVID-19 symptoms and a shorter duration of sickness (10 studies), lower mortality rates (3 studies), and decreased hospitalization and length of stay (3 studies). Six in vitro studies found that probiotics were beneficial against SARS-CoV-2 through antiviral effects. There were only two studies that found probiotics to be ineffective or caused negative effects when consumed in COVID-19 patients. \u0000Conclusion: Available evidence supports the antiviral role of probiotics on prevention and treatment of COVID-19. The antiviral potential of Lactobacillus paracasei metabolite PlnE and PlnFagainst SARS-CoV-2 may explain the effectiveness of probiotics on COVID-19.","PeriodicalId":21412,"journal":{"name":"Russian Journal of Infection and Immunity","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73031919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization and validation of flow cytometry method for quantification of SARS-COV-2 antigen reactive T-cells","authors":"O. Strizhakova, A. S. Pershin, A. A. Kazarov, I. Lyagoskin, Yana A. Bahareva, Alexander P. Vasil'ev, Yu. A. Nikonova, I. Egorova, Rahim R. Shukurov, R. Khamitov","doi":"10.15789/2220-7619-oav-2084","DOIUrl":"https://doi.org/10.15789/2220-7619-oav-2084","url":null,"abstract":"A proper and representative monitoring of SARS-CoV-2 herd immunity including a long-term health impact on recovered patients and vaccinated individuals is of great importance. For this, a monitoring campaign should assesses both humoral and T-cell immune arms. Upon that, analyzing antigen specific-cell activation and cellular phenotype are informative. \u0000We developed a flow cytometry method for detection of intracellular IFN-producing antigen-reactive T cells after exposure of human peripheral blood mononuclear cells (PBMC) to SARS-CoV-2 virus antigens. The method was validated according to the following characteristics: sensitivity, specificity, precision, and robustness. \u0000We used positive samples from donors recovered from COVID-19 and negative samples from donors who had no contact with COVID-19 patients and lacking antibodies to SARS-CoV-2. All samples were tested by laboratory methods. Peripheral blood mononuclear cells were isolated from donor blood by centrifugation in a Ficoll density gradient. Specific T cells were stimulated with S-protein as well as N, M, ORF3a, and ORF7a protein peptides to count IFN--producing T cells by flow cytometer. The data were statistically analyzed. AUC level was determined. The predictive value of the method was considered acceptable when AUC was greater than 0.7. Precision was considered acceptable if the coefficient of variation did not exceed 20%. Robustness was confirmed for frozen and freshly prepared PBMC samples. \u0000Based on the validation, the suitability of the method \"Evaluation of antigen-reactive T cells that produce intracellular IFN- in response to SARS-CoV-2 virus antigens by flow cytometry\" was confirmed. The method allows for reliable data that was used to characterize standard control samples for internal quality control of TigraTest SARS-CoV-2 kits.","PeriodicalId":21412,"journal":{"name":"Russian Journal of Infection and Immunity","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90925050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A MULTIFACTORIAL IMPACT ON RESPIRATORY MORBIDITY IN PEOPLE OF REPRODUCTIVE AGE AND EFFECTIVENESS OF NATOPHERINE- AND ANDROPHERINE-CONTAINING ODORANTS REGARDING A NON-SPECIFIC RESISTANCE OF THE NASAL MUCOSA AND LOWERED MORBIDITY OF RESPIRATORY INFECTIONS","authors":"A. Bedareva, E. Shabaldina, Evgenia Astaf'eva, A. Chuyanova, A. Shabaldin","doi":"10.15789/2220-7619-ami-8048","DOIUrl":"https://doi.org/10.15789/2220-7619-ami-8048","url":null,"abstract":"Prevention of respiratory infections during epidemic periods is an important task of modern health care in Russia and the world. One such approach may be the use of volatile substances capable of activating nonspecific resistance of the nasal mucosa. Long-term application of synthetic analogues of female pheromones to young men leads to a decrease in the frequency of respiratory diseases, an increase in the levels of secretory immunoglobulins, and in persons with allergic diseases, to a decrease in the levels of eosinophilia in the peripheral blood. Based on this, the aim of the study was set - to study the influence of catamnesis factors and indicators reflecting the functioning of the mucosal immunity of the nasal mucosa, its nonspecific resistance, on the formation of frequent respiratory morbidity; as well as to evaluate the effectiveness of odorants with natopherine and andropherine in relation to the dynamics of these indicators and to reduce the frequency of respiratory infections in young people. Materials and methods. A survey of 46 conditionally healthy boys and girls, in the age range from 18 to 22 years, was carried out. The frequency of respiratory morbidity, catamnesis factors, and the results of non-invasive studies (cytokine concentrations in the nasopharyngeal aspirate and rhinocytograms). Within 30 days, all young people were applied to the nasolabial fold synthetic analogues of male (andropherine) and female (natopherine) pheromones (andropherine for young woman, natopherine for young man). A re-examination with the above mentioned studies was carried out on the 14th and 30th day of the experiment. The frequency of respiratory infections was reassessed one year after the start of the experiment. Results. The study showed a number of predictors and protectors of frequent and long-term respiratory morbidity in young people. The predictors of this condition were catamnestics markers of allergic anomalies of the constitution, behavioral factors (smoking), as well as immune indicators of allergy (IL-4) and mucociliary clearance deficiency. A monthly course of applications on the skin of the nasolabial fold of odorants based on synthetic analogues of male and female pheromones (steranes) has a positive effect both on the nonspecific resistance of the nasal mucosa, on the immune parameters of its functioning. After a month of using steranes, complete decontamination of the nasal mucosa from coccal microorganisms occurs. A year after the experiment, there are no frequently and long-term ill young people. This indicates a significant clinical effect of the applied odorants.","PeriodicalId":21412,"journal":{"name":"Russian Journal of Infection and Immunity","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74345218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"VIRUS-LIKE PARTICLE ENDOCYTOSIS PATHWAYS AND CAPTURED ANTIGEN PRESENTATION","authors":"","doi":"10.15789/2220-7619-vpe-8045","DOIUrl":"https://doi.org/10.15789/2220-7619-vpe-8045","url":null,"abstract":"The proteins of many viruses can be assembled into strictly organized structures virus-like particles bearing antigens of the original viruses and may also be artificially decorated with antigens of other pathogens. These particles contain no viral genome and lack infectivity but can be highly immunogenic and therefore being actively used for vaccine development. Undoubtedly, while designing vaccines, it is necessary to take into account information about the interaction of vaccine components with immune system particularly antigen-presenting cells. This is especially important for virus-like particles because, like other nanometer-sized particles, they can enter antigen-presenting cells using various endocytosis pathways. The latter exploit multiple receptors, generate endocytic vesicles of different sizes, and, most importantly, are associated with varying fates of captured material. Here we review the mechanisms of phagocytosis, macropinocytosis, clathrin-mediated endocytosis, rapid endophilin-mediated endocytosis, and several endocytic pathways associated with lipid rafts. The data are presented on the relationship between various endocytic pathways and sorting of absorbed cargo in early endosomes as well as enzymatic degradation of the late endosomes contents. We also describe the mechanisms of distribution of absorbed antigens within antigen-presenting cells to be loaded onto the class I and II major histocompatibility complex molecules. The data are presented on the endocytosis of various viruses during cell infection, as well as a comparative analysis of the endocytosis pathways for virus-like particles and related viruses. It has been noted that virus-like particles, along with the absorption pathway specific for parent virus, can rely on additional endocytic pathways to be also artificially targeted at the selected endocytic receptor and relevant absorption pathway. It allows to select or design particles with optimal endocytosis and antigen presentation to induce a protective immune response upon vaccination. It should be assumed that most prophylactic vaccines require particles that are well engulfed by antigen presenting cells and direct material to endolysosomal degradation, or particles whose uptake directs material to both late and static early endosomes, making antigens available for \"direct\" and cross presentations. Finally, we discuss virus-like particles for the delivery of drugs or genetically engineered constructs, as well as optimal endocytic pathways that should protect the payload of these particles from endolysosomal degradation.","PeriodicalId":21412,"journal":{"name":"Russian Journal of Infection and Immunity","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86338483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A clinical case of SARS-CoV-2 infection complicated by nephrogenic pulmonary edema and COVID-associated pneumonitis, alveolitis","authors":"O. Vorobeva, Natalya E. Gimaldinova, L. P. Romanova","doi":"10.15789/2220-7619-acc-1985","DOIUrl":"https://doi.org/10.15789/2220-7619-acc-1985","url":null,"abstract":"COVID-19 is a highly transmissible disease with severe course especially in patients with nephrogenic hypertensive disease and chronic kidney disease due to a higher incidence of all-type infections than in the general population. The aim of the study is to describe a clinical case of SARS-CoV-2 infection complicated by nephrogenic pulmonary edema and COVID-associated pneumonitis, alveolitis. Description of the case. Patient K.S., born in 1975, was hospitalized 24 hours after symptom onset at emergency hospital due to complaints of increased blood pressure up to 180200/110120 mm Hg, temperature up to 38.7C, dry cough, feeling of heaviness in the chest, change in urine color. PCR smear for SARS-CoV-2 was positive. Computed tomography revealed a pattern of bilateral COVID-associated pneumonitis, alveolitis, with 75% involvement. The electrocardiogram revealed signs of left ventricular myocardial hypertrophy. Ultrasound examination showed numerous cysts in the kidneys. Urinalysis at admission: leukocytes 499, erythrocytes 386. Glomerular filtration rate (CKD-EPI: 29 ml/min/1.73 m2) and corresponds to stage IV of chronic kidney disease. Coagulogram: fibrinogen: 32.3 (1.64.0) g/l, D-dimer: 663 (0250). Despite the treatment, the patients condition worsened, the phenomena of cardiopulmonary and renal insufficiency increased, which led to a fatal outcome. During a virological study of sectional material: SARS-CoV-2 coronavirus RNA was found in the lung and kidneys. Signs of bilateral COVID-associated pneumonitis, alveolitis with diffuse cellular infiltrates in combination with changes in the alveolar apparatus, signs of pulmonary edema were revealed. Heart-related signs swelling of the interstitium, fragmented muscle fibers, some of them hypertrophied, a wave-like deformation of cardiomyocytes, blurring of the transverse striation. Arteries with thickened sclerosed walls. In the kidneys diffuse damage to the proximal tubules of the nephron with areas of cortical and proximal necronephrosis, areas of fibrinoid swelling. Conclusion. The cause of death of a 45-year-old patient was a severe course of bilateral COVID-associated pneumonitis, alveolitis, which contributed to the development of renal medullary hypoxia and type 1 cardiorenal syndrome, which led to early nephrogenic pulmonary edema.","PeriodicalId":21412,"journal":{"name":"Russian Journal of Infection and Immunity","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84179049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of the intestinal microflora in children receiving anti-tuberculosis therapy","authors":"E. Bryukhacheva, Y. Zakharova, L. Otdushkina, T. V. Pyanzova","doi":"10.15789/2220-7619-cot-2037","DOIUrl":"https://doi.org/10.15789/2220-7619-cot-2037","url":null,"abstract":"The progress in investigating microbiome in children and adolescents as well as its impact on maintaining health is considered one of the most significant achievements of modern medicine. In children with various diseases, the qualitative and quantitative indicators of the intestinal microflora change, which causes the development of dysbiosis. The assessing changes in intestinal microflora in children during treatment with anti- tuberculosis drugs has retained its importance and relevance due to insufficient data in the field, which requires a more detailed understanding of this problem. The presented article assesses the state of gut microbial biocenosis in children receiving anti-tuberculosis treatment. The purpose of the study: to study the microbial landscape of the colonic contents in children receiving anti-tuberculosis therapy. Materials and methods. The study included 24 children with verified tuberculosis of the respiratory organs receiving treatment in a 24-hour hospital of the Kuzbass Clinical Phthisiopulmonological Medical Center named after I.F. Kopylova. The study of intestinal microflora was carried out by a quantitative bacteriological method. The material of the study was feces collected in a sterile disposable container, which was delivered to the Department of Microbiology, Immunology and Virology of the Federal State Budgetary Educational Institution of the Russian Ministry of Health. Results. Dysbiosis with predominance of microecological disorders of II (41.7%) and III (37.3%) degrees was detected in all children receiving anti-tuberculosis therapy. The microbial landscape of the intestine was characterized by a decrease in the quantitative content of representatives of the obligate flora Bifidobacterium spp. in 66.7% of children and Lactobacillus spp. in 45.8%, an increase in the number of facultative microorganisms: E. coli lac+ and Citrobacter freundii in 12.5%. Maintaining a full-fledged and active intestinal normobiota is possible by ensuring its dynamic control in children with tuberculosis during treatment. The results of studying the characteristics of the microbiota suggest that patients need probiotic therapy during the treatment of tuberculosis. Conclusion. Microecological disorders in children with tuberculosis therapy were characterized by a decrease in the quantitative content of representatives of obligate flora and an increase in the number of facultative microorganisms. Also in the material, various types of fungi of the genus Candida were identified.","PeriodicalId":21412,"journal":{"name":"Russian Journal of Infection and Immunity","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87701580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotyping of rickettsias circulating in the territories of the Altai Republic and Khabarovsk krai","authors":"S. Shtrek, N. Rudakov, S. Shpynov, Alexey V. Sannikov, I. Samoylenko, L. D. Shchuchinova, O. Trotsenko, Anna G. Dragomeretskaya, E. V. Matushchenko","doi":"10.15789/2220-7619-gor-2014","DOIUrl":"https://doi.org/10.15789/2220-7619-gor-2014","url":null,"abstract":"The aim of the study was to verificate of the causative agent of Siberian tick-borne typhus (STT) upon ixodic tick mixed infection on the territory of the Altai Republic and Khabarovsk Krai using an integrated molecular biological approach. Materials and methods. The material for the study was presented as 304 imago of ixodic mites of six species. The ticks were collected on the territory of the Altai Republic and the Khabarovsk Krai in the years 20142022. Rickettsia DNA was identified by two-round PCR using genus- and species-specific primers for gltA and ompA genes, followed by sequencing and using PCR reagent kits RealBest DNA R. sibirica/R. heilongjiangensis (Vector-Best, Novosibirsk). Results. The detection of rickettsia in ticks in the Altai Republic was 82.6% (CI: 69.196.1), in the Khabarovsk Krai 53.1% (CI: 44.961.3). The species composition of rickettsias in various species of ixodic mites in these territories, endemic to Siberian tick typhus, is characterized by the presence of mixed forms. Rickettsia sibirica and R. raoultii were found in ticks Dermacentor nuttalli, Haemaphysalis concinna, D. silvarum. Only Candidatus R. tarasevichiae were detected in Ixodes persulcatus, I. pavlovsky mites and I. pavlovsky/persulcatus hybrids. The DNA of R. heilongjiangensis was found in H. mites. concinna in the Khabarovsk Krai. The DNA of Candidatus R. tarasevichiae is also present in the ticks of D. nuttalli and H. concinna from the Altai Republic. The DNA of the classic pathogenic species, R. sibirica, was detected in ticks D. nuttalli and H. concinna in the Altai Republic, ticks D. silvarum, H. concinna and H. japonica douglasi in the Khabarovsk Krai. In addition, in the Khabarovsk Krai, the DNA of R. heilongjiangensis was detected in H. concinna ticks, and a DNA fragment common to R. sibirica subsp. mongolitimonae and R. conorii was detected in H. japonica douglasi ticks, which requires further study. Candidatus R. tarasevichiae is common in Ixodes mites (I. persulcatus, I. pavlovsky and their hybrids), in the Altai Republic in D. nuttalli and H. concinna. R mites. raoultii was detected in ticks of the genus Dermacentor D. nuttalli in the Altai Republic and D. silvarum in the Khabarovsk Krai. Conclusions. During molecular biological monitoring of rickettsias in endemic territories, the detection of R. raoultii masks the presence of the etiological agent of STT R. sibirica. This phenomenon makes it possible to explain the high incidence of STT in the studied territories, with the rare detectability of R. sibirica DNA during molecular biological screening in ixodic mites.","PeriodicalId":21412,"journal":{"name":"Russian Journal of Infection and Immunity","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85293785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Features of lymphocyte subset composition in neonates born to mothers suffered from COVID-19 at different stages of pregnancy","authors":"E. Inviyaeva, Julia A Kosolapova, L. Krechetova, Valentine V. Vtorushina, Mziya I. Makieva, V. Zubkov","doi":"10.15789/2220-7619-fol-2098","DOIUrl":"https://doi.org/10.15789/2220-7619-fol-2098","url":null,"abstract":"The relationship between the incidence of COVID-19 in pregnant women who have had a coronavirus infection at different gestational ages and the health status of paired neonates is of great interest. However, no sufficient convincing data fully reflecting features of subsequent neonatal period, the state of the immune system in this category of children, affecting characteristics of postnatal period have been accumulated. Based on this, it underlies the relevance of the current study aimed at investigating parameters of clinical and immunological state of neonatal health after paired mothers recovered from COVID-19 at different gestational ages. The prospective study included 131 women and 132 children. The main group consisted of women (n = 61) who had COVID-19 during pregnancy and paired newborns (n = 62) at gestational age (GA) of 3741 weeks, the comparison group women without laboratory-confirmed COVID-19 during pregnancy (n = 70) and paired newborns (n = 70) of similar gestational age. While analyzing the anamnesis of the patients, no significant differences in somatic and obstetric-gynecological diseases were found. Analyzing course of pregnancy revealed that low molecular weight heparins were significantly more often applied in the main group. The term and frequency of delivery by caesarean section in pregnant women in the main group did not significant differ from that of the control group. No significant difference in the frequency of causes accounting for the severity of the condition of neonates in paired mothers with COVID-19 at different trimester of gestation was found. Investigating lymphocyte subset composition, neutrophil phagocytic activity, and IgG class antibodies specific to SARS-CoV-2 was carried out. It was found that lymphocyte subset profile in newborns from paired mothers with COVID-19 at different trimesters of gestation differed only in the level of NK cells (CD56+) in children born to mothers recovered from COVID-19 in the first trimester. In this study, in general, no severe perinatal outcomes in newborns from paired mothers with COVID-19 during pregnancy were documented. No cases of moderate or severe maternal COVID-19 were observed. Therefore, further prospective studies are needed to assess an impact of COVID-19 severity on maternal and fetal birth outcomes and clarify optimal management of pregnant women in such cases.","PeriodicalId":21412,"journal":{"name":"Russian Journal of Infection and Immunity","volume":"153 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73468621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}