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Revue canadienne de biologie最新文献

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Relationships between hormone-induced calcium release and 86rubidium uptake stimulation in starfish oocytes. 海星卵母细胞激素诱导钙释放与铷摄取刺激的关系。
Revue canadienne de biologie Pub Date : 1979-09-01
P Guerrier, M Moreau, M Dorée
{"title":"Relationships between hormone-induced calcium release and 86rubidium uptake stimulation in starfish oocytes.","authors":"P Guerrier,&nbsp;M Moreau,&nbsp;M Dorée","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>86Rubidium+ uptake, but not 86Rubidium efflux, is strongly stimulated after addition of the meiosis inducing hormone 1-methyladenine (1-MeAde) to prophase blocked oocytes of the starfish Marthasterias glacialis. This stimulation is a transient process which does not require the continuous presence of 1-MeAde and is elicited within 1 minute of contact. 1-MeAde and its biologically active structural analogs fully stimulate Rb+ uptake at concentrations which are about two orders of magnitude lower than those required to trigger meiosis reinitiation but which already release underthreshold levels of Ca2+ from the inner part of the plasma membrane. External Ca2+ concentrations effective in triggering meiosis reinitiation also stimulate Rb+ influx, while drugs like D600, theophyllin and caffein which suppress the hormone induced Ca2+ release, simultaneously preclude the stimulation of Rb+ uptake. Dithiothreitol (DTT) which mimicks 1-MeAde action in releasing Ca2+ and inducing meiosis acts both on the efflux and on active and passive Rb+ influxes. Ouabain, the classical inhibitor of the Na+, K+ pump does not preclude meiosis reinitiation under the influence of 1-MeAde, its agonists of mimetics. It suppresses the active component of Rb+ uptake both in control or stimulate oocytes. When applied only in preincubation before starting the hormone treatment, it cannot however inhibit the stimulation of Rb+ uptake, while basal pump inhibition is preserved. These results demonstrate that stimulation of the active Rb+ or K+ transport is not indispensable to meiosis reinitiation. They suggest moreover that the hormone induced Ca2+ release from the plasma membrane may be responsible for unmasking new ouabain sensitive transport sites.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"38 3","pages":"145-56"},"PeriodicalIF":0.0,"publicationDate":"1979-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11719444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Urine production in the halothane anesthetized rabbit. 氟烷麻醉家兔的尿量。
Revue canadienne de biologie Pub Date : 1979-09-01
J C Russell, B F Litchfield, D C Secord
{"title":"Urine production in the halothane anesthetized rabbit.","authors":"J C Russell,&nbsp;B F Litchfield,&nbsp;D C Secord","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Urine and blood parameters were studied in rabbits subjected to anesthesia and abdominal surgery. Conscious control animals both fasted and fed to which water was freely available were used for comparison. During anesthesia and operation one group of animals were given no intravenous fluid, other groups were infused with normal saline at a rate of 0.5 ml, 1 ml, or 2 ml per minute. The results demonstrated that an intravenous saline infusion of 0.5 ml per minute, maintains an essentially normal state of hydration and is beneficial to rabbits during anesthesia and surgery. However, with such an infusion, the rabbits do not produce urine under the experimental conditions. Higher rates of infusion are associated with a declining heart rate and blood pressure, abnormal urine, and metabolic acidosis. We conclude that the rabbit is an inappropraite species for renal studies involving anesthesia and surgery.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"38 3","pages":"177-83"},"PeriodicalIF":0.0,"publicationDate":"1979-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11720637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Salinity adaptation in the snake eel Pisoodonophis boro (Ham-buch). 蛇鳗对盐度的适应。
Revue canadienne de biologie Pub Date : 1979-09-01
A Subramanian
{"title":"Salinity adaptation in the snake eel Pisoodonophis boro (Ham-buch).","authors":"A Subramanian","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The snake eel Pisoodonophis Boro (Family Ophichthidae) burrows, causing leaks in the bunds and damaging the salterns and paddy fields adjoining estuaries. The animal's capability to withstand brine in salterns and fresh water in paddy fields was determined from laboratory experiments. The eel tolerated salinities ranging from deionized water to 75%. Adaptation to fresh water as well as full strength seawater was completed within 24 h. The concentration of sodium and chloride in the blood varied by 10--20% on transfer from seawater to fresh water. P. boro can survive in paddy fields but not in salterns. However, their occurrence in both is described as a rare phenomenon and not obligatory.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"38 3","pages":"167-71"},"PeriodicalIF":0.0,"publicationDate":"1979-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11719446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Confirmation of the presence of uteroglobin in tubal secretions]. [确认输卵管分泌物中存在子宫红蛋白]。
Revue canadienne de biologie Pub Date : 1979-09-01
L Guilbert-Blanchette, R D Lambert, A Bélanger
{"title":"[Confirmation of the presence of uteroglobin in tubal secretions].","authors":"L Guilbert-Blanchette,&nbsp;R D Lambert,&nbsp;A Bélanger","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The acrylamide gel electrophoresis of rabbit serum, of rabbit tubal secretions at the oestrus stage (STO) and at the luteal stage (STL), followed by immunodiffusion with anti-serum, was done to detect any tubal proteins which were not found in the rabbit serum. The anti-rabbit STO goat anti-serum was absorbed with rabbit plasma in order to purify antibodies to proteins found specifically in tubal secretions. This technique revealed the presence of a protein (STx) in STO and STL but not in the rabbit serum. The electrophoretic migration (Rf = 0.72) of the tubal protein (STx) is similar to uteroglobin (Rf = 0.74). The tubal protein was detected immunologically only in tissues where uteroglobin had been found by other workers: lungs, semen, Fallopian tubes and uterine secretions (5 days post-ovulation); further more a complete identity for the precipitating line existed between these extracts. These results corroborate the presence of uteroglobin or of a very similar protein in oestrus of luteal tubal secretions and in some other rabbit tissues.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"38 3","pages":"189-93"},"PeriodicalIF":0.0,"publicationDate":"1979-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11336606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Localization of a gene responsible for ozone sensitivity in Escherichia coli]. [大肠杆菌对臭氧敏感基因的定位]。
Revue canadienne de biologie Pub Date : 1979-06-01
G Côtes, Y S Chung
{"title":"[Localization of a gene responsible for ozone sensitivity in Escherichia coli].","authors":"G Côtes,&nbsp;Y S Chung","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A gene, ozrC, responsible for sensitivity to ozone in Escherichia coli, was localized on the E. coli chromosome between argEH and metA by means of analysis of cotransduction frequencies of the gene ozrC with certain gene markers in the malB region of the chromosome.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"38 2","pages":"97-9"},"PeriodicalIF":0.0,"publicationDate":"1979-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11595054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pear malic enzyme: some physical and immunochemical properties. 梨苹果酸酶:一些物理和免疫化学性质。
Revue canadienne de biologie Pub Date : 1979-06-01
C J Hartmann, A G Drouet
{"title":"Pear malic enzyme: some physical and immunochemical properties.","authors":"C J Hartmann,&nbsp;A G Drouet","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Some physical and immunochemical characteristics of NADP malic enzyme from climacteric pear (Pyrus communis L. var Passe-Crassane) are described. The enzyme which has a molecular weight of about 224 000 d and a sedimentation coefficient of about 9.6 S, is formed by the association of four sub-units. We were not able to isolate isozymes by electrophoresis or by immunochemistry.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"38 2","pages":"101-4"},"PeriodicalIF":0.0,"publicationDate":"1979-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11334860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ultrastructural correlates of luminescence in Porichthys photophores. II. Effects of metabolic inhibitors. 波鱼荧光体发光的超微结构相关。2代谢抑制剂的作用。
Revue canadienne de biologie Pub Date : 1979-06-01
M Anctil
{"title":"Ultrastructural correlates of luminescence in Porichthys photophores. II. Effects of metabolic inhibitors.","authors":"M Anctil","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Prolonged, bright luminescent glows in Porichthys photophores are elicited by administration of 2,4-dinitrophenol (DNP) and potassium cyanide (KCN). Ultrastructural alterations of varicose nerve endings precede photocyte changes during such luminescent activity. Common alterations of nerve profiles include mitochondrial disruptions, flattening and depletion of synaptic vesicles, formation of large vacuolar cisternae, and invaginations in the contour of axolemma. Protracted luminescent activity in response to DNP results in depletion of photocyte vesicle material while vesicle and ER membranes accumulate and coil inside coalesced vesicle pools, and photocyte microvilli disappear completely. Although similar photocyte alterations are initially observed in KCN treated luminescing photophores, the early extinction of the response to KCN is related to deleterious, irreversible effects of this chemical on photocytes. These observations, along with some pharmacological manipulations, indicate that at least DNP acts initially and primarily on neural structures, probably the mitochondria, to induced transmitter release and consequent photocyte activity. Based on this and earlier studies, a chain of subcellular events leading to light emission of Porichthys photophores is proposed and discussed.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"38 2","pages":"81-96"},"PeriodicalIF":0.0,"publicationDate":"1979-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11698424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Light and electron microscopic studies on the pineal tract of rainbow trout, Salmo gairdneri. 虹鳟松果体束的光镜和电镜研究。
Revue canadienne de biologie Pub Date : 1979-06-01
Y Omura
{"title":"Light and electron microscopic studies on the pineal tract of rainbow trout, Salmo gairdneri.","authors":"Y Omura","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The pineal tract of rainbow trout from the pineal end vesicle to the posterior commissure was studied by light and electron microscopy. Five types of nerve fibres (photoreceptor basal process, ganglion cell dendrite, electron-lucent fibre and synaptic vesicles, myelinated and unmyelinated axons) and two modes of synapses (photoreceptor basal process ganglion cell dendrite and axon terminal with synaptic vesicles-photoreceptor basal process synapses) are distinguishable in the proximal region of end vesicle. The two distinct synaptic associations with the photoreceptor basal process suggest two different (excitatory and inhibitory) control of pineal sensory activity. At the distal portion of stalk about two thousand nerve fibres converge into dorsal and ventral bundles. Posterior to the habenular commissure several small branches run out laterally from the ventral bundles to the basal margin of the ependyma, but not into the habenular commissure. The dorsal bundle passes through the dorsal side of the subcommissural organ and runs ventral to the posterior commissure. The pineal tract is composed of unmyelinated axons, electron-lucent nerve fibres and myelinated axons. The number of fibres increases throughout the stalk and reaches the maximum number at the opening of pineal lumen to IIIrd ventricle, however, the number of fibres then decreases through the subcommissural organ and posterior commissure. This increase and decrease of nerve fibres suggest the continuous participation of axonal fibres of pineal nerve cells and the ramification or branching of pineal tract, respectively.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"38 2","pages":"105-18"},"PeriodicalIF":0.0,"publicationDate":"1979-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11698419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of estradiol benzoate on bone cell population kinetics in fetal rat fibulae cultured in vitro. 苯甲酸雌二醇对体外培养胎鼠腓骨骨细胞群动力学的影响。
Revue canadienne de biologie Pub Date : 1979-06-01
M Liskova-Kiar
{"title":"Effect of estradiol benzoate on bone cell population kinetics in fetal rat fibulae cultured in vitro.","authors":"M Liskova-Kiar","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"38 2","pages":"55-61"},"PeriodicalIF":0.0,"publicationDate":"1979-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11698421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Abnormal characteristics of the blood from chick embryos maintained in "shell-less" culture. 在“无壳”培养中维持的鸡胚血液的异常特征。
Revue canadienne de biologie Pub Date : 1979-06-01
B Burke, R Narbaitz, S Tolnai
{"title":"Abnormal characteristics of the blood from chick embryos maintained in \"shell-less\" culture.","authors":"B Burke,&nbsp;R Narbaitz,&nbsp;S Tolnai","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Chick embryos were maintained in shell-less culture up to a total age of 15 days. The composition of their blood was analyzed together with the blood from coontrol embryos of comparable degree of differentiation. The blood from cultured embryos had lower hematocrit values; their serum contained a reduced concentration of proteins, phospholipids and total calcium and an increased concentration of inorganic phosphorus. In view of the reduced concentration of proteins and phospholipids, the concomitant hypocalcemia must represent, at least in part, a reduction in the binding capacity of the serum and not only a decrease in the ionic fraction of serum calcium.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"38 2","pages":"63-6"},"PeriodicalIF":0.0,"publicationDate":"1979-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11777459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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