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Conformational changes in antigen-stimulated lymphocytes detected with 1-anilino-8-naphthalene sulphonate. 1-苯胺-8-萘磺酸钠检测抗原刺激淋巴细胞构象变化。
Revue canadienne de biologie Pub Date : 1978-03-01
J Vaillier, D Vaillier
{"title":"Conformational changes in antigen-stimulated lymphocytes detected with 1-anilino-8-naphthalene sulphonate.","authors":"J Vaillier,&nbsp;D Vaillier","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The hydrophobic fluorescent probe, 1-anilino-8-naphthalene sulphonate (ANS), has been used to study conformational changes of mouse antigen-stimulated lymphocytes in vivo. Studies revealed that early conformational changes appear in Bovine Serum Albumin-- or Sheep Erythrocytes-- stimulated splenic and thymic lymphocytes. These conformational changes are detected by fluorescence intensity changes, when ANS is bound to lymphocytes. The kinetic studies further indicate that the course of conformational changes may vary considerably depending on antigens.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"37 1","pages":"27-34"},"PeriodicalIF":0.0,"publicationDate":"1978-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11875831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The mecanism of cementing line formation in the bones of cestrum-fed chicks. 乳喂养雏鸡骨中胶结线形成的机制。
Revue canadienne de biologie Pub Date : 1978-03-01
L F Bélanger, R Narbaitz
{"title":"The mecanism of cementing line formation in the bones of cestrum-fed chicks.","authors":"L F Bélanger,&nbsp;R Narbaitz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Chicks fed a rachitogenic diet for five weeks after hatching were then treated with a daily oral dose of 1,000 I.U. Vitamin D3 or a 1% addition to the feed of powdered leaves of Cestrum diurnum for periods of 1, 2, 4, 8, 16 and 30 days. Comparative studies were made on stained sections, microradiographs of undermineralized sections and alpharadiographs of demineralized sections. The present dose of Cestrum diurnum caused at first, a rapid maturation and mineralization of the epiphyseal cartilage and an intense growth and osteocytic osteolysis in the diaphysis of the tibia and femur. After 8 days however, growth decreased and the diaphysis gradually became petrotic. Under these conditions, the osteocytes degenerated and died. The areas of polysaccharide-rich, low density matrix which surrounded them, decreased gradually to become cementing lines, persistent after 30 days.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"37 1","pages":"5-14"},"PeriodicalIF":0.0,"publicationDate":"1978-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11424458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of estradiol benzoate on the proliferation of osteogenic cells in fetal rat fibulae cultured in vitro. 苯甲酸雌二醇对体外培养胎鼠腓骨成骨细胞增殖的影响。
Revue canadienne de biologie Pub Date : 1978-03-01
Liskova-Kiar
{"title":"Effect of estradiol benzoate on the proliferation of osteogenic cells in fetal rat fibulae cultured in vitro.","authors":"Liskova-Kiar","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The influence of estradiol benzoate on the proliferation of bone cells was investigated in vitro by radioautography. The fibulae from 19-day fetal rats were pulse-labeled with 3H-thymidine after various periods of culture on estrogen-supplemented (5 microgram/ml) or control media. The analysis of radioautographs revealed that the cell division was restricted to osteogenic cells of undifferentiated appearance. No label was found in osteoblasts or in osteoclasts. At the time of explantation, about 30% of osteogenic cells were labeled. However, this percentage varied considerably after different intervals of culture. In the control bones, it decreased during first 12 hours to 15% and then rose slowly to attain a preculture value after 48 hours. In the presence of estradiol, the initial decline of labeled cells was less pronounced. The percentage recovered to a preculture level already after 24 hours and continued to increase thereafter. The numbers of labeled osteogenic cells, in estrogen-treated bones, were significantly higher than in controls already after 12 hours of culture. It is concluded that the fetal rat bones undergo in vitro a period of adaptation to a new environment as indicated by a transient decrease of dividing cells. Estradiol benzoate appears to shorten this period by stimulating the proliferation of undifferentiated cells.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"37 1","pages":"35-41"},"PeriodicalIF":0.0,"publicationDate":"1978-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11875832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Production of colicins V and V2 in vitro and in vivo. Study of their inhibitory action on phagocytosis by peritoneal macrophages]. 体外和体内黏菌素V和V2的产生。它们对腹腔巨噬细胞吞噬抑制作用的研究[j]。
Revue canadienne de biologie Pub Date : 1977-12-01
G Ozanne, L G Mathieu, J P Baril
{"title":"[Production of colicins V and V2 in vitro and in vivo. Study of their inhibitory action on phagocytosis by peritoneal macrophages].","authors":"G Ozanne,&nbsp;L G Mathieu,&nbsp;J P Baril","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In recent years, a possible relationship between pathogenicity and colicinogeny in some Escherichia coli colicin V-producing strains had been inferred. In our laboratory, we have elaborated a simple in vitro method for the production of colicin V free of large, non dialyzable macromolecules and presumably of bacterial endotoxin. This allows study of the effects of colicin V in vivo without an undesirable added physiological response of the experimental animal to endotoxin. All the Col V+ strains we have studied displayed a greater ability to survive in the peritoneal cavity of mice than the Col V- strains. Also, we have detected colicin V in peritoneal fluids of agonizing mice injected with Col V+ strains. Phagocytosis by peritoneal macrophages seemed to be inhibited in vitro in the presence of colicin V. Colicin V is not toxic in vivo in low concentration, after intraperitoneal or intravenous injection but it may favor the multiplication and the invasiveness of the strains that produce it.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"36 4","pages":"307-16"},"PeriodicalIF":0.0,"publicationDate":"1977-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11556647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Perspectives in sensory-ecological studies of higher vertebrates. 高等脊椎动物感觉生态学研究展望。
Revue canadienne de biologie Pub Date : 1977-12-01
P Pirlot
{"title":"Perspectives in sensory-ecological studies of higher vertebrates.","authors":"P Pirlot","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It is firstly recalled that, among Vertebrates, in peripheral sense-organs on the one hand, in encephalic relays and terminals on the other hand, not only the types but also the numbers of neurons, are determining factors for the kinds of relations that exist between organisms and environments. It is thus necessary to consider the quantitative developments of relay-stations and the volumes of brain-components when studying such relations. The very concept of sense-organ may even have to be broadened, because it should include central transmission structures along with peripheral starting points. It is stressed that progresses achieved by Vertebrates in the course of their history by quantitative increases of either terminals or relays should be labeled either as specializations or as evolutions, for these two concepts must not be confused.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"36 4","pages":"337-50"},"PeriodicalIF":0.0,"publicationDate":"1977-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11813151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Encephalization and quantitative brain composition in bats in relation to their life-habits. 蝙蝠的脑化和定量脑组成与其生活习惯的关系。
Revue canadienne de biologie Pub Date : 1977-12-01
P Pirlot, J Pottier
{"title":"Encephalization and quantitative brain composition in bats in relation to their life-habits.","authors":"P Pirlot,&nbsp;J Pottier","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A quantitative analysis of the brains of 43 bat species is presented. Eleven brain components were studied. The species were arranged according to seven distinct dietary groups and it was found that the relative development of the principal components is related to those groups. The importance of neocorticalization as a reflection of evolution of all the bats in contrast to specialization in some species is stressed. This work gives a clearer view of Chiropteran progressiveness or primitiveness: the insectivorous forms occupy the least advanced, although most specialized, level; the vampires, the carnivorous species and the flying foxes are at the top of the scale. The importance of behaviour and the relative development of the central nervous system in the hierarchial classification of mammals is stressed.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"36 4","pages":"321-36"},"PeriodicalIF":0.0,"publicationDate":"1977-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11813150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Communication by harmonization of electric organ discharge frequencies by Eigenmannia virescens (Sternopygidae, Pisces). 通过本征曼(胸骨蝶科，双鱼座)电器官放电频率的协调进行通信。
Revue canadienne de biologie Pub Date : 1977-12-01
P Gaddis
{"title":"Communication by harmonization of electric organ discharge frequencies by Eigenmannia virescens (Sternopygidae, Pisces).","authors":"P Gaddis","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Spectrum analysis of the combined electric organ discharges of confined, interacting Eigenmannia virescens individuals indicated 1) the relative frequency position of each individual in the group's \"chord\" was invariably conserved, i.e. the fish do not cross each other in frequency; 2) the ratios of 2:3 and 3:4 between the frequencies of two individuals were conserved over intervals of many days; 3) changes in group size induced frequency shifts that were often quite dramatic. It is suggested that this \"chord\" may function in the communication of information regarding a group's spatial position and its social rank relations.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"36 4","pages":"317-20"},"PeriodicalIF":0.0,"publicationDate":"1977-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11813149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Resistance of earthworms Lumbricus terrestris and Allolobophora turgida to the fungicide captan 50 W.P]. [地蚓和异球虫对杀菌剂captain 50 W.P的抗性研究]。
Revue canadienne de biologie Pub Date : 1977-12-01
R G Léger, G J Millette
{"title":"[Resistance of earthworms Lumbricus terrestris and Allolobophora turgida to the fungicide captan 50 W.P].","authors":"R G Léger,&nbsp;G J Millette","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Two species of earthworms were kept in Muck, Chicot and Ste-Sophie soils treated with captan 50 W.P. solutions of 700, 1 400 and 2 800 ppm. After a 42-day treatment period, L. terrestris had a 95% survival and A. turgida 100% survival. Using a gas chromatograph method, no captan was detected in tissue extracts of exposed earthworms. Based on the survival rate and the undetected presence of captan in earthworm tissues, we concluded that soil residues of this product are unlikely to be harmful to the animal's ecology.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"36 4","pages":"351-3"},"PeriodicalIF":0.0,"publicationDate":"1977-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11813152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Induction of antituberculous immunity by polysaccharidic contaminants of crude ribosomal vaccines isolated from Mycobacterium bovis, strain BCG. 牛分枝杆菌卡介苗粗核糖体疫苗多糖污染物诱导抗结核免疫的研究。
Revue canadienne de biologie Pub Date : 1977-09-01
V Portelance, R P Boulanger, R Brasseur
{"title":"Induction of antituberculous immunity by polysaccharidic contaminants of crude ribosomal vaccines isolated from Mycobacterium bovis, strain BCG.","authors":"V Portelance,&nbsp;R P Boulanger,&nbsp;R Brasseur","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Crude ribosomal fractions isolated from the BCG strain of Mycobacterium bovis are capable of inducing antituberculous immunity in CF-1 mice. These crude preparations contain varying amounts of polysaccharides in addition to ribosomal ribonucleic acid and proteins. Whereas these latter two constituents of the crude fractions were found inactive, high levels of antituberculous immunity were induced by a ribosomal sub-fraction significantly enriched with polysaccharides. These results strongly suggest that polysaccharides play an important role in the induction of antituberculous immunity in CF-1 mice immunized with crude ribosomal fractions isolated from the BCG strain and that if ribosomal proteins and/or RNA are of a certain importance, their role would be secondary (complexes providing physical support to polysaccharides, adjuvant, etc.).</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"36 3","pages":"265-75"},"PeriodicalIF":0.0,"publicationDate":"1977-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12113610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Victorien Fredette, 1913-1975]. [维多利亚·弗雷德特，1913-1975]。
Revue canadienne de biologie Pub Date : 1977-09-01
A Frappier
{"title":"[Victorien Fredette, 1913-1975].","authors":"A Frappier","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"36 3","pages":"191-2"},"PeriodicalIF":0.0,"publicationDate":"1977-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11549445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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