Induction of antituberculous immunity by polysaccharidic contaminants of crude ribosomal vaccines isolated from Mycobacterium bovis, strain BCG.

Abstract

Crude ribosomal fractions isolated from the BCG strain of Mycobacterium bovis are capable of inducing antituberculous immunity in CF-1 mice. These crude preparations contain varying amounts of polysaccharides in addition to ribosomal ribonucleic acid and proteins. Whereas these latter two constituents of the crude fractions were found inactive, high levels of antituberculous immunity were induced by a ribosomal sub-fraction significantly enriched with polysaccharides. These results strongly suggest that polysaccharides play an important role in the induction of antituberculous immunity in CF-1 mice immunized with crude ribosomal fractions isolated from the BCG strain and that if ribosomal proteins and/or RNA are of a certain importance, their role would be secondary (complexes providing physical support to polysaccharides, adjuvant, etc.).