J G Bisaillon, R Beaudet, L Lafond, S A Saheb, M Sylvestre
{"title":"Antigonococcal and antibacterial spectra of some bacterial isolates of the urogenital flora.","authors":"J G Bisaillon, R Beaudet, L Lafond, S A Saheb, M Sylvestre","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The antigonococcal and antibacterial spectra of bacterial isolates of the urogenital flora selected for their in vitro interference of Neisseria gonorrhoeae growth were determined on solid medium. A broad antigonococcal spectrum was found for all the selected isolates when they were tested against gonococcal virulent (T1) strains, penicillinase producing strains and strains belonging to the auxotypes NR, Thi-, Pro- Hyx-, Pro- Meth- Thp-, Arg- Meth- and Arg- Hyx- Ura-. Except for the group D streptococci, all the selected isolates particularly the coagulase negative staphylococci showed a narrow interference spectrum towards aerobic and anaerobic bacterial representatives of the normal urogenital flora. The selected isolates inhibited also the growth of N. meningitidis and N. catarrhalis meaning that they produce antineisserial rather than antigonococcal activities. The crude preparations isolated from cultures of Micrococcus sp. No. 2 and 42, and Acinetobacter sp. No. 13 on solid medium showed similar antigonococcal and antibacterial spectra as those observed with the basal spot/lawn method. These inhibitory activities were characterized for stability to extreme of temperature and pH values, and for susceptibility to different enzyme treatments. Based on ultrafiltration, differences in molecular size were observed between the inhibitors. These substances appear different from the previously reported gonococcal inhibitors of bacterial origin.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"40 2","pages":"215-27"},"PeriodicalIF":0.0,"publicationDate":"1981-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17844798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Impairment of testicular maturation in potassium depleted pre-pubertal rats.","authors":"K Sarkar, G A Kinson, R Narbaitz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>When maintained on a potassium deficient diet 21 day-old weanling rats ceased to grow. At the end of 3 weeks, the testes were small and the diameter of seminiferous tubules was reduced. The reduction of the tubular diameter resulted mainly from a decrease in the number of cells, chiefly spermatids. An occasional tubule contained a few scattered spermatozoa. Ultrastructurally, the cytoplasm of Sertoli cells contained an increased number of lipid droplets as well as phagocytosed degenerative cells. Spermatids, conspicuously fewer in number, showed abnormal forms and did not mature beyond the early acrosomic phase. It appeared then, that potassium deficiency in immature rats not only arrested growth but largely prevented spermatogenesis. This probably resulted from a widespread inhibition of pituitary trophin function. Also, the deleterious effects on spermatids could be due to local lack of potassium which is present in high concentration in normal seminiferous tubular lumen.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"40 2","pages":"203-14"},"PeriodicalIF":0.0,"publicationDate":"1981-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18291804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Establishment of continuously in vitro growing cell lines of med-fly (Ceratitis capitata wied.).","authors":"R Cavalloro","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The Mediterranean fruit fly, Ceratitis capitata Wiedemann, represents an extremely harmful insect pest of considerable agricultural and economic interest. As part of the study of this species, two diploid cell lines (2n = 12) of the Med-fly were established in vitro on two different culture media. The two lines were obtained from embryonic eggs 24 (+/- 1) hours old and were called CEC CC 128 and CEC CC 130. Detailed descriptions are given of the techniques used for the preparation of the primary cultures, their development and characterization, the choice and composition of the culture media and the intrinsic characteristics of the lines obtained. Indications of other elements are given, such as different cells, non cellular vesicles and glands containing symbiotic bacteria, observable in the first phase of development of the culture, before obtaining continuously growing cell lines. The doubling time of the population for both cell lines were estimated at 22 hours and the interval between successive sub-cultures at 7 days.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"40 2","pages":"181-6"},"PeriodicalIF":0.0,"publicationDate":"1981-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18291802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of dilution rates, animal species and instruments on the spectrophotometric determination of sperm counts.","authors":"M Rondeau, M Rouleau","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Using semen from bull, boar and stallion as well as different spectrophotometers, we established the calibration curves relating the optical density of a sperm sample to the sperm count obtained on the hemacytometer. The results show that, for a given spectrophotometer, the calibration curve is not characteristic of the animal species we studied. The differences in size of the spermatozoa are probably too small to account for the anticipated specificity of the calibration curve. Furthermore, the fact that different dilution rates must be used, because of the vastly different concentrations of spermatozoa which is characteristic of those species, has no effect on the calibration curves since the dilution rate is shown to be artefactual. On the other hand, for a given semen, the calibration curve varies depending upon the spectrophotometry used. However, if two instruments have the same characteristic in terms of spectral bandwidth, the calibration curves are not statistically different.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"40 2","pages":"173-80"},"PeriodicalIF":0.0,"publicationDate":"1981-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18291801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A quantitative approach to the marsupial brain in an eco-ethological perspective.","authors":"P Pirlot","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Using a volumetric method and the classical allometry equation, the author investigates the possibility of a relationship between the size of total brain or brain-components and the ecology of ethology of 29 marsupials species from South-America and Australia. He shows that there is a relation between brain and neocortex volumes, and the taxonomic groups characterized by definite life-habits. New light is shed upon the evolution of marsupials showing that, while the living didelphids most probably are the closest to the ancestral type, some other groups such as the dasyurids and peramelids include even more primitive forms. This points to a very early separation of American and Australian marsupials and to a long independent evolution, perhaps dating back to immediately pre-marsupial times.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"40 2","pages":"229-50"},"PeriodicalIF":0.0,"publicationDate":"1981-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18291805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Isolation of influenza viruses in different species of bird in Canada in 1978].","authors":"A Boudreault, J Lecomte","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>As part of the international program on the ecology of influenza virus in animals sponsored by W.H.O., 357 influenza A viruses isolated from 2 293 cloacal samples collected from ducks and other bird species in Eastern Canada during the 1978 season were characterized antigenically. Seven hemagglutinin (Hsw 1, H2, H3, Hav2, Hav4, Hav6, Hav7) and six neuraminidase subtypes (N1, N2, Neq2, Nav1, Nav5, Nav6) in 18 different combinations were found. A comparison with viruses isolated during previous seasons indicates that subtypes do change from year-to-year and from place-to-place. Isolation of few viruses from passerine birds requires additional studies to determine if these species are truly infected with influenza virus in nature. This large reservoir of influenza A viruses circulating at the same time in ducks may well be involved in the appearance of new viruses in other species, including humans.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"40 1","pages":"139-45"},"PeriodicalIF":0.0,"publicationDate":"1981-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17231484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Number of neurons and synapses in the visual cortex of different species].","authors":"M Colonnier, J O'Kusky","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The number of neurons under 1 mm2 of visual cortex (area 17) is about 200 000 in monkey and man, and it varies between 45 000 and 70 000 in non-primates which have been studied. The number per hemisphere increases with the surface of area 17, passing from less than 1 million in mouse to about 538 million in man. The number of synapses under 1 mm2 of visual cortex has been estimated by different authors at between 480 million (mouse) and 1270 million (rat) : the number per hemisphere increases with brain size from 32 billion in rat to 3 084 billion (x10(9)) in man. The number of synapses per neurons tends to be higher in species with fewer neurons per mm3. Our laminar study in monkey shows this correlation at the level of each lamina : those having the largest number of neurons per mm3 have the least number of synapses per neuron.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"40 1","pages":"91-9"},"PeriodicalIF":0.0,"publicationDate":"1981-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18258542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Interactions between retino-collicular and retino-cortical pathways at the level of the lateral geniculate nucleus].","authors":"S Molotchnikoff","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Interactions between retino-collicular and retino-cortical pathways at the level of the lateral geniculate nucleus were studied in anesthetized and paralyzed rabbits. In a first series of experiments the cortico-geniculate influence was analyzed by cortical depression. The disruption of cortical functions led to a specific abolition of the geniculate responses to right-left movement of the stimulating spot. The response to the opposite direction was unaffected. In a second series of experiments the stimulus presentation was triggered by a spontaneous collicular spike. This resulted in a significant modification of the geniculate responses recorded simultaneously suggesting that the superior colliculus is capable of modifying the transmission that occurs through the geniculate. Data are discussed in view of the visuo-motor integration.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"40 1","pages":"77-84"},"PeriodicalIF":0.0,"publicationDate":"1981-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18259772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[A comparison between orienting rapid eye movements accompanying active or passive head movement in the cat].","authors":"D Guitton, R M Douglas","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Gaze is shifted by means of eye saccades which, in most instances, are synchronized with head rotations. During eye-head movements performed by cats in the dark, most of the rapid eye movements start after the head has begun to move (mean time lag was 45 msec). This pattern resembles that observed when the cat, as a whole, is suddenly rotated passively and consists of a short lasting vestibularly induced slow phase component followed by a rapid eye movement that takes the eye in the same direction as the head. We have compared the passively and actively induced eye-head movements. The rapid eye movements are similar in both cases in that they terminate at a fixed position (\"goal\") in the orbit irrespective of the eye's starting position. They differ primarily in the fact that the eccentricity of the \"goal\" during active head rotations increases much more rapidly with velocity than it does during the passive condition. The results suggest that the rapid eye movement that accompanies an active head movement in the dark is not simply a vestibularly induced quick phase.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"40 1","pages":"69-75"},"PeriodicalIF":0.0,"publicationDate":"1981-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18259771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Effects of uterine secretions on embryonic development].","authors":"I Cantin, R D Lambert, Y J Laberge, L Ferland","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Rabbit embryos at the stage of one-cell and morula were incubated in uterine secretions from rat. The uterine fluid was aspirated from uterus of freshly sacrificed estrus animals. We obtained a very good development of the morulas (mean of the maximum diameter : 0.79 +/- 0.02 mm); however only 35% of the one cell rabbit embryos reached the stage of 16 cells. This consequently suggest a uterine tropism in the rats similar to that previously found for the rabbit.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"40 1","pages":"147-9"},"PeriodicalIF":0.0,"publicationDate":"1981-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18259766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}