Journal of Microbial Systematics and Biotechnology最新文献_第2页

Biosorption chrome (Cr) and dyes using biosorbent in the modified tea bag 利用生物吸附剂吸附改性茶包中的铬和染料

Journal of Microbial Systematics and Biotechnology Pub Date : 2019-06-24 DOI: 10.37604/jmsb.v1i1.20

S. Lestari, R. S. Dewi, E. Wibowo, Atang

引用次数: 0

Inventory of macrofungi at peat swamp forest area, Kapuas Hulu, West Kalimantan 西加里曼丹Kapuas Hulu泥炭沼泽林区大型真菌清查

Journal of Microbial Systematics and Biotechnology Pub Date : 2019-06-24 DOI: 10.37604/jmsb.v1i1.17

Noverita, T. Setia

引用次数: 0

Antagonistic activity of three Aspergillus isolates against Fusarium wilt of banana 3株分离曲霉对香蕉枯萎病的拮抗活性

Journal of Microbial Systematics and Biotechnology Pub Date : 2019-06-24 DOI: 10.37604/jmsb.v1i1.16

I. Hidayat, Lita Citra Dewi, D. Sukmawati

引用次数: 2

Antibiofilm and antimicrobial activities of papaya (Carica papaya L.) and stevia (Stevia rebaudiana Bertoni) leaf extracts against three biofilm-forming bacteria 木瓜(Carica papaya L.)和甜叶菊(stevia reaudiana Bertoni)叶提取物对三种生物膜形成细菌的抗菌活性研究

Journal of Microbial Systematics and Biotechnology Pub Date : 2019-06-24 DOI: 10.37604/jmsb.v1i1.18

A. Hastuty

引用次数: 6

PCR-based Specific Detection of Bacillus in Liquid Organic Fertilizer 基于pcr的液体有机肥中芽孢杆菌特异性检测

Journal of Microbial Systematics and Biotechnology Pub Date : 2019-06-24 DOI: 10.37604/jmsb.v1i1.21

I. Hidayat, N. Laili, D. Agustiyani, S. Antonius

引用次数: 1

Mycorrhiza stimulates Rhizobium infection in Paraserianthes falcataria (L.) I.C. Nielson under Hg contamination 菌根刺激镰形副花根瘤菌感染I.C.尼尔森公司受到汞污染

Journal of Microbial Systematics and Biotechnology Pub Date : 1900-01-01 DOI: 10.37604/jmsb.v3i1.70

I. Latifah, I. Idris, T. P. Napitupulu, A. Z. N. Ikhwani, Gunawan Ruhiyat, I. N. Sumerta, A. Kanti, F. Y. Amandita, I. Sudiana

引用次数: 0

Analysis of the SARS-CoV-2 envelope (E), nucleocapsid (N), and non-structural protein12 (nsp12) genes from COVID-19 patients in West Java 西爪哇地区COVID-19患者的SARS-CoV-2包膜(E)、核衣壳(N)和非结构蛋白12 (nsp12)基因分析

Journal of Microbial Systematics and Biotechnology Pub Date : 1900-01-01 DOI: 10.37604/jmsb.v3i1.66

A. Fibriani, Irin Annisa Evitayani, Gusti Ayu Prani Pradani, Rebecca Stephanie, Ema Rahmawati, R. B. Ristandi, Cut Nur Cinthia Alamanda, Rifky Waluyajati Rachman, Rini Robiani, Isak Solihin

{"title":"Analysis of the SARS-CoV-2 envelope (E), nucleocapsid (N), and non-structural protein12 (nsp12) genes from COVID-19 patients in West Java","authors":"A. Fibriani, Irin Annisa Evitayani, Gusti Ayu Prani Pradani, Rebecca Stephanie, Ema Rahmawati, R. B. Ristandi, Cut Nur Cinthia Alamanda, Rifky Waluyajati Rachman, Rini Robiani, Isak Solihin","doi":"10.37604/jmsb.v3i1.66","DOIUrl":"https://doi.org/10.37604/jmsb.v3i1.66","url":null,"abstract":"According to World Health Organization, as of January 2021, Indonesia is the only Southeast Asian country in which COVID-19 is still occurring in community transmission. West Java is one of the provinces holding the highest positive cases number. With the envelope (E), nucleocapsid (N), and non-structural protein 12 (nsp12) being the target genes of SARS-CoV-2 diagnostic kits and several antiviral drugs, the study of genetic variations has become relevant and greatly important. Out of 267 oro-nasopharyngeal swab specimens that were previously confirmed positive for COVID-19 in qPCR diagnostic test in Laboratorium Kesehatan Provinsi Jawa Barat, ten samples with acceptable qualities were selected and three samples were sequenced using Sanger sequencing. Nonsynonymous mutations were observed in the envelope gene (L21F) and in the nucleocapsid genes (R203K, G204R, A211S, and S193I). Phylogenetic analysis showed that samples were clustered with other sequences carrying identical mutations, but clustered non-discriminatively with all sequences when carrying no mutation. No pattern in geographical areas and clades, except for R203K-G204R for being a marker for the GR clade. Protein structure analysis showed that mutations observed did not change the hydrophobicity and the secondary structure of the nucleocapsid, while stability change (ΔΔG) showed that all mutations, aside from the R203K-G204R, have neutral effect on the protein stability. Therefore, it can be concluded that mutations observed in this experiment did not impart preference to disperse in certain geographical areas or cause any significant structural change in the protein.","PeriodicalId":212822,"journal":{"name":"Journal of Microbial Systematics and Biotechnology","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114101212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and application of nitrogen-fixing and indole-3-acetic acid producing bacteria A13 in Oil Palm (Elaeisguineensis Jacq.) seedling 油棕(Elaeisguineensis Jacq.)幼苗固氮产吲哚-3-乙酸细菌A13的鉴定及应用

Journal of Microbial Systematics and Biotechnology Pub Date : 1900-01-01 DOI: 10.37604/jmsb.v3i1.77

I. Isti'anah, Nisa Rachmania, A. Tjahjoleksono

{"title":"Characterization and application of nitrogen-fixing and indole-3-acetic acid producing bacteria A13 in Oil Palm (Elaeisguineensis Jacq.) seedling","authors":"I. Isti'anah, Nisa Rachmania, A. Tjahjoleksono","doi":"10.37604/jmsb.v3i1.77","DOIUrl":"https://doi.org/10.37604/jmsb.v3i1.77","url":null,"abstract":"Oil palm plantations have a good prospect in Indonesia. One of the efforts to improve the productivity of oil palm plantation is the application of bacteria as biological fertilizer. The research was conducted to characterize and apply the nitrogen-fixing and indole-3-acetic acid producing bacteria in oil palm seedlings. The bacteria was isolated from soil samples which taken from Taman Nasional Bukit Dua Belas (TNBD) Jambi. Nitrogen free bromthymol blue (NFB) is used as media for nitrogen-fixing bacterial isolation. Selected isolate named A13 had an ability to form white pellicle on the surface of the semisolid medium, increased the pH, and changed the color of medium from green to blue Isolate A13 was identified as Gram-negative bacteria and had a rods shape. Analysis of 16S rRNA gene sequence showed that isolate A13 had a similarity with Pseudochrobactrum assacharolyticum. Hypersensitivity assay on tobacco leaves showed that isolate A13 was not a pathogen. During 48 hours of incubation, isolate A13 produced a maximum of IAA at the 24th hour of incubation. Isolate A13 produced 0.675 ppm of ethylene/hour in Acetylene Reduction Assay and 69,839 ppm of IAA in HPLC methods. This was the first report on nitrogen fixation and IAA production by Pseudochrobactrum assacharolyticum and its application in the soil of oil palm seedlings. Application of isolate A13 in oil palm seedling increased significantly the number of lateral roots, stem diameter, and height of plants","PeriodicalId":212822,"journal":{"name":"Journal of Microbial Systematics and Biotechnology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114988200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthesis and profiling of single cell carotenoids of Phaf ia rhodozyma in waste-based cultivation media 红土酵母菌单细胞类胡萝卜素在废物培养基中的生物合成及特性分析

Journal of Microbial Systematics and Biotechnology Pub Date : 1900-01-01 DOI: 10.37604/jmsb.v3i1.61

Arif Nurkanto, R. Handayani, I. Purnaningsih, Mia Kusmiati, Ninu Setianingrum, M. Mulyadi, Endang Kusdiyanti, A. Dinoto

{"title":"Biosynthesis and profiling of single cell carotenoids of Phaf ia rhodozyma in waste-based cultivation media","authors":"Arif Nurkanto, R. Handayani, I. Purnaningsih, Mia Kusmiati, Ninu Setianingrum, M. Mulyadi, Endang Kusdiyanti, A. Dinoto","doi":"10.37604/jmsb.v3i1.61","DOIUrl":"https://doi.org/10.37604/jmsb.v3i1.61","url":null,"abstract":"In order to investigate the feasibility of low-cost media for producing well- characterized single-cell carotenoid, the study on biosynthesis and profiling carotenoid in the yeast of Phaf ia rhodozyma was carried out. We have successfully identified the profiles of single-cell carotenoids of P. rhodozyma, which was cultivated in glucose-based medium (MG), molasses-based medium (MM), and coconut water-based medium (MC). Cells were separately cultured in those media under aerobic batch culture system to obtain the carotenoid profiles based on high performance liquid chromatography (HPLC) analysis. The results showed that medium composition strongly affects the profiles of P. rhodozyma carotenoids represented by ratio of astaxanthin and beta-carotene (ratio A/B). Astaxanthin was highly synthesized in cells cultivated in MG with ratio A/B as much of 20:1. On the other hand, MM and MC produced a lower ratio A/B than MG as much of 0.4:1 and 0.2:1, respectively. In addition, carotenoids profiles were detected more diverse when this yeast species was cultivated in two waste-based media. This study provided a basic physiological knowledge of P. rhodozyma cells for carotenoid biosynthesis using potential low cost cultivation m","PeriodicalId":212822,"journal":{"name":"Journal of Microbial Systematics and Biotechnology","volume":"287 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121070356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of Clove essential oil (Syzygium aromaticum) against the growth of dandruff scalps-causing fungal pathogen using Kirby- Bauer method in vitro 用Kirby- Bauer法体外研究丁香精油对头皮屑头皮真菌病原菌生长的影响

Journal of Microbial Systematics and Biotechnology Pub Date : 1900-01-01 DOI: 10.37604/jmsb.v3i1.78

K. Khusnul, Puput Putuh Anisa, D. P. Virgianti

{"title":"Effect of Clove essential oil (Syzygium aromaticum) against the growth of dandruff scalps-causing fungal pathogen using Kirby- Bauer method in vitro","authors":"K. Khusnul, Puput Putuh Anisa, D. P. Virgianti","doi":"10.37604/jmsb.v3i1.78","DOIUrl":"https://doi.org/10.37604/jmsb.v3i1.78","url":null,"abstract":"One of the strongest contributing factors in dandruff scalps is the presence of fungal pathogen or called as Malassezia. Several studies have found a connection between Malassezia and scalp health, one of which is caused by pathogenic fungal infections including Pityrosporum ovale, Microsporum gypseum, and Candida albicans. Clove flowers are known to contain eugenol and its derivative compounds which have antimicrobial, antifungal, antiseptic, and local anesthetic activity. This research aims to determine the effect of clove flower essential oil (Syzygium aromaticum) against some dandruff-causing fungi. The research method was to test the inhibitory power of clove flower essential oil using the diffusion method (Kirby Bauer technique). Samples of pathogenic fungi were cultured on SDA media (Saboroud Dextrose Agar) and given discs that had been given several concentrations of clove flower essential oil. The concentrations used were 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, positive control using 2% ketoconazole and negative control using virgin coconut oil (VCO). The results showed a significant effect between the ability of essential oils to inhibit the growth of some fungi that cause dandruff. The treatment of clove flower essential oil against M. gypseum at the concentration of 10% has an inhibitory ability of 33,05 mm and at the concentration of 100% amounted to 63.95 mm. Meanwhile, the inhibition percentage in P. ovale with a concentration of 10% and 100% of extract were 14,75 mm and 46,15 mm, respectively. The inhibition rate in C. albicans with the concentration of 10% extract and 100% of extract was 13,5 mm and 39,35 mm. The research shows that clove flower essential oil inhibits the growth of several pathogenic fungal in vitro.","PeriodicalId":212822,"journal":{"name":"Journal of Microbial Systematics and Biotechnology","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122028763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0