Journal of Microbial Systematics and Biotechnology最新文献

Cover and Table of Content JMSB Vol 3, No 1 (2021) JMSB第3卷第1期(2021)封面和目录

Journal of Microbial Systematics and Biotechnology Pub Date : 2021-08-31 DOI: 10.37604/jmsb.v3i1.82

C. Jmsb

引用次数: 0

Effect of ethanol extract from Karuk leaf (Piper sarmentosum Roxb.) on the growth of Malassezia furfur in vitro 芦芦叶乙醇提取物对毛马拉色菌体外生长的影响

Journal of Microbial Systematics and Biotechnology Pub Date : 2020-12-30 DOI: 10.37604/jmsb.v2i2.59

K. Khusnul, Asti Kusmayanti, L. Rahman, N. Ratnaningtyas

{"title":"Effect of ethanol extract from Karuk leaf (Piper sarmentosum Roxb.) on the growth of Malassezia furfur in vitro","authors":"K. Khusnul, Asti Kusmayanti, L. Rahman, N. Ratnaningtyas","doi":"10.37604/jmsb.v2i2.59","DOIUrl":"https://doi.org/10.37604/jmsb.v2i2.59","url":null,"abstract":"In Indonesia, there are numerous therapeutic plants found. Some of the plants used in herbal medicine are Karuk leaf (Piper sarmentosum Roxb.) belong to the Piperaceae family. Karuk leaf has chemical contents such as saponins, polyphenols, flavonoids, and essential oils and many tests are carried out on several bacteria, but testing of fungi is rarely studied. This study aims to determine the ethanol extract activities from karuk leaf in inhibiting the growth of the Malassezia furfur fungus and to determine its minimum inhibition by using the Kirby-Bauer method. The study was conducted by an experimental method of the M. furfur fungus using the Kirby-Bauer method. The ethanol extract from karuk leaf was made in various concentrations and tested on 0.5 McFarland fungus by diffusion on Sabouraud Dextrose Agar (SDA). The results of this analysis showed that the ethanol extract of Karuk leaf could inhibit the M. furfur fungus at a concentration of 30% by 5.3 mm, 40% by 6.6 mm, 50% by 7.6 mm, 60% by 11.3 mm, 70% by 12.5 mm, 80% by 15.6 mm, 90% by 17.4 mm, and 100% by 19.5 mm. Based on the results of the study and the data analysis, it can be concluded that several concentrations of ethanol extract of Karuk leaf affect the growth of M. furfur in vitro.","PeriodicalId":212822,"journal":{"name":"Journal of Microbial Systematics and Biotechnology","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129028125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extraction, characterization, and biological toxicity of β-glucans from Saccharomyces cerevisiae isolated from ragi 豚草中酿酒酵母β-葡聚糖的提取、鉴定及生物毒性研究

Journal of Microbial Systematics and Biotechnology Pub Date : 2020-12-30 DOI: 10.37604/jmsb.v2i2.62

I. Ramadhani, D. Larissa, Y. Yuliani, M. Amir, Kusmiati Kusmiati

{"title":"Extraction, characterization, and biological toxicity of β-glucans from Saccharomyces cerevisiae isolated from ragi","authors":"I. Ramadhani, D. Larissa, Y. Yuliani, M. Amir, Kusmiati Kusmiati","doi":"10.37604/jmsb.v2i2.62","DOIUrl":"https://doi.org/10.37604/jmsb.v2i2.62","url":null,"abstract":"β-glucan is a homopolysaccharide with biological activities that are beneficial to health as an immunostimulant, anti-inflammatory, anti-diabetic, anti-cholesterol, and many more. β-glucan extraction results from yeast require characterization related to this bioactive quality, such as β-glucan weight, monomer analysis, functional groups, and cytotoxicity assay. Four Saccharomyces cerevisiae isolates were isolated from three local ragi samples, namely the SC-1, SC-2, SC-3, and SAF from instant ragi. This study aimed to obtain the best candidate of S. cerevisiae isolates to produce high β-glucan levels and low protein levels and to test the potential for cytotoxicity. The four isolates were rejuvenated on potato dextrose agar (PDA), then inoculated into the liquid glucose yeast peptone (GYP) fermentation medium for six days. Saccharomyces cerevisiae cells were extracted by neutralizing acid-base, dried and weighed as a crude β-glucan (mg per 300 mL). The highest yield was SC-2 (818 mg), followed by SC-3 (726 mg), SAF (597 mg), and SC-1 (433 mg). The presence of –OH (alcohol), -C-C-C- (alkane), and –R-O-R- (ether) groups were showed using FTIR characterization. Glucose equivalent β-glucan levels and protein levels were determined using a UV-Vis spectrophotometer. The results showed that β-glucan SC-1 gave the best results with glucose equivalent β-glucan levels of 4,865% and protein levels of 3,804%. The crude β-glucan toxicity test using the brine shrimp lethality test (BSLT) method shows that the β-glucan of the SAF strain has LC50 cytotoxicity of 114.8 ppm followed by β-glucan cytotoxicity from local ragi LC50 was SC-2 (323.5 ppm), SC-1 (331.1 ppm), and SC-3 (354.8 ppm). Therefore, based on the results, SC-1 isolate obtained the highest β-glucan crude and the lowest protein content was SC-2. The β-glucan of SAF extract had the highest toxicity properties based on the IC50 value.","PeriodicalId":212822,"journal":{"name":"Journal of Microbial Systematics and Biotechnology","volume":"298 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115870436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Culturable bacterial abundance in Volvariella volvacea cultivation medium and characterization of its bacteria 草粪培养基中可培养细菌丰度及其细菌特性研究

Journal of Microbial Systematics and Biotechnology Pub Date : 2020-12-30 DOI: 10.37604/jmsb.v2i2.52

M. Masrukhin, I. Saskiawan

{"title":"Culturable bacterial abundance in Volvariella volvacea cultivation medium and characterization of its bacteria","authors":"M. Masrukhin, I. Saskiawan","doi":"10.37604/jmsb.v2i2.52","DOIUrl":"https://doi.org/10.37604/jmsb.v2i2.52","url":null,"abstract":"Straw mushroom (Volvariella volvacea) is one of the popular edible fungi in Indonesia. Previous researches showed the correlation among the type of substrate, substrate quality, and its composting process to the microbial community, yield, and biological efficiency. The aim of the research is to analyze the culturable bacteria abundance in straw mushroom cultivation medium, characterize the bacteria in several stages of mushroom cultivation and investigate the interaction between V. volvacea with its resident bacteria. Samples were taken from mushroom farmers in Subang and Karawang regencies, Indonesia. The materials for cultivation medium are the mixture of cotton and paddy straw and the pasteurization was performed at 65-70°C for 7 hours. The result shows the abundance of the bacteria in most of the cultivation stages is relatively similar i.e. 108 CFU/g, except in 15 days after inoculation (DAI), the bacterial abundance is lower i.e.6.24 x 107 CFU/g. Twenty-five isolates were obtained and Gram-positive bacteria is the dominant bacteria found in the cultivation medium, especially rod-shaped Gram-positive bacteria. According to co-culture assay there are nine isolates that decrease the growth rate and clearly inhibit mycelial growth. The other 10 isolates have lower inhibitory activity, and 6 isolates have no inhibitory activity to the mycelial growth. C38 isolates have the highest mycelial growth inhibition. It belongs to rod-shaped Gram positive group of bacteria which isolated from the early stage of V. volvacea cultivation medium (5 DAI).","PeriodicalId":212822,"journal":{"name":"Journal of Microbial Systematics and Biotechnology","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114162360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The influence of biocarrier of Aspergillus niger and Trichoderma harzianum toward vegetative growth of sorghum in the field experiment 田间试验研究了黑曲霉和哈茨木霉生物载体对高粱营养生长的影响

Journal of Microbial Systematics and Biotechnology Pub Date : 2020-12-30 DOI: 10.37604/jmsb.v2i2.60

A. Sugiharto, T. P. Napitupulu, M. Sudiana

{"title":"The influence of biocarrier of Aspergillus niger and Trichoderma harzianum toward vegetative growth of sorghum in the field experiment","authors":"A. Sugiharto, T. P. Napitupulu, M. Sudiana","doi":"10.37604/jmsb.v2i2.60","DOIUrl":"https://doi.org/10.37604/jmsb.v2i2.60","url":null,"abstract":"Compared to other cereal crops, sorghum has a higher drought tolerance trait. However, efforts are needed to increase the productivity of sorghum, particularly in drought marginal land. One strategy to be implemented is the utilization of soil microorganisms formulated with biocarrier. Therefore, the aim of this study was to evaluate the influence of the fungal strain Aspergillus niger and Trichoderma harzianum formulated with compost and zeolite as biocarrier towards vegetative growth of sorghum. The field experiment was designed as a randomized block designed, factorial pattern with 4 replications. The first factor was selecting biocarrier, namely zeolite, compost, and a mixture of zeolite: compost (1:1). The second factor was the fungal inoculants, A. niger, and T. harzianum. The observed parameter was the growth profile of sorghum during vegetative growth, including stalk diameter and height. The results showed that the type of biocarrier, as well as the fungal strains did influence the growth of sorghum. The highest stalk diameter and height of sorghum were obtained after application of A. niger formulated with a mixture of zeolite: compost (1:1), with 17% and 41.2% higher than control, respectively. This condition shows that a mixture of zeolite and compost is seemingly able to create better micro-ecological conditions for fungal microbes to function effectively. Therefore, our findings suggested the addition of zeolite to compost for the application of biocarrier in the field experiment.","PeriodicalId":212822,"journal":{"name":"Journal of Microbial Systematics and Biotechnology","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117266864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Development of a dimer-based screening system for dimerization inhibitor of HIV-1 protease 基于二聚体的HIV-1蛋白酶二聚化抑制剂筛选系统的建立

Journal of Microbial Systematics and Biotechnology Pub Date : 2020-12-30 DOI: 10.37604/jmsb.v2i2.42

I. D. A. P. Dwipayana, Y. M. Syah, Reza Aditama, Feraliana Feraliana, A. Fibriani

{"title":"Development of a dimer-based screening system for dimerization inhibitor of HIV-1 protease","authors":"I. D. A. P. Dwipayana, Y. M. Syah, Reza Aditama, Feraliana Feraliana, A. Fibriani","doi":"10.37604/jmsb.v2i2.42","DOIUrl":"https://doi.org/10.37604/jmsb.v2i2.42","url":null,"abstract":"An in vitro dimer-based screening system (DBSS) for selecting new HIV-1 protease dimerization inhibitor candidates from natural compounds had been established. This system utilizes a fusion between HIV-1 protease and dimer binding domain of AraC protein (proteaseHIV1-AraCDBD) where fluorescence signal will be emitted in the presence of HIV-1 protease inhibitor. However, this screening system had not been evaluated. Therefore, this study was aimed to evaluate it in recombinant Escherichia coli culture. The expression of proteaseHIV1-AraCDBD fusion gene was observed for 18 hours. Its crude lysate isolation was done once every 3 hours and analyzed using SDS PAGE. To test the DBSS, darunavir was used as positive control, and Nigella sativa extract (JH3) was used as the test compound. The results of SDS PAGE analysis on crude lysates presented a ~24.2 kDa band, which was the predicted size of the proteaseHIV1-AraCDBD fusion protein based on its amino acid sequence. The growth curve and protein expression profiles revealed that the 15 hours was the optimum culture age to be used in the screening system. Darunavir testing in DBSS showed an increase in fluorescence signal compared to the negative control. The same increase in fluorescence signal was also obtained from the JH3 compound test. In conclusion, DBSS could be used as an assay to screen for new HIV-1 protease inhibitors, and the JH3 compound demonstrated the ability to inhibit HIV-1 protease dimerization.","PeriodicalId":212822,"journal":{"name":"Journal of Microbial Systematics and Biotechnology","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121543916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Cover JMSB Vol 2, No 1 (2020) 封面JMSB第2卷第1期(2020)

Journal of Microbial Systematics and Biotechnology Pub Date : 2020-07-31 DOI: 10.37604/jmsb.v2i1.44

C. Jmsb

引用次数: 0

Cover JMSB Vol.1 No.2 (2019) 封面JMSB 2019年第1卷第2期

Journal of Microbial Systematics and Biotechnology Pub Date : 2019-12-30 DOI: 10.37604/jmsb.v1i2.32

C. Jmsb

引用次数: 0

Cover JMSB Vol 1, No 1 (2019) 封面JMSB第1卷第1期(2019)

Journal of Microbial Systematics and Biotechnology Pub Date : 2019-06-26 DOI: 10.37604/jmsb.v1i1.15

Jmsb Jmsb

引用次数: 0

Nickel (Ni) reduction in Sorowako post-mining soil through application of mycorrhiza Acaulospora sp. associated with Canavalia ensiformis L. 应用与Canavalia ensiformis L.相关的Acaulospora菌根对Sorowako矿区后土壤镍的还原作用。

Journal of Microbial Systematics and Biotechnology Pub Date : 2019-06-24 DOI: 10.37604/jmsb.v1i1.19

M. Akib, K. Mustari, T. Kuswinanti, S. A. Syaiful, Syatrawati, Z. Kumalawati

{"title":"Nickel (Ni) reduction in Sorowako post-mining soil through application of mycorrhiza Acaulospora sp. associated with Canavalia ensiformis L.","authors":"M. Akib, K. Mustari, T. Kuswinanti, S. A. Syaiful, Syatrawati, Z. Kumalawati","doi":"10.37604/jmsb.v1i1.19","DOIUrl":"https://doi.org/10.37604/jmsb.v1i1.19","url":null,"abstract":"The nickel (Ni) content in a post-mining soil of Pomalaa mines reached 14,200 mg.kg-1 and became a limiting factor in the plant growth process. A Ni reduction in the soil by using phyto-accumulator such as Jack bean (Canavalia ensiformis L.) can be improved by combining it with arbuscular mycorrhizal (AM) fungi. The purpose of this study was to determine the effect of the mycorrhizal fungus Acaulospora sp. on the efficiency of Ni reduction by C. ensiformis. This experiment was carried out by using a randomized block design with three different treatments, include: 1) C. ensiformis without Acaulospora sp. inoculation (negative control), 2) C. ensiformis inoculated with indigenous Acaulospora sp. and 3) C. ensiformis inoculated with non-indigenous Acaulospora sp. The study was conducted in the nursery that belongs to PT. Vale Indonesia Tbk., Sorowako, South Sulawesi, Indonesia. The results showed that highest nickel accumulation was found in the root inoculated with indigenous Acaulospora sp. (9500 mg.kg-1), followed by stem (1400 mg.kg-1), leaf and pod (1300 mg.kg-1), seed (1200 mg.kg-1), and flower (1100 mg.kg-1). This study indicates that application of the indigenous Acaulospora sp. can improve C. ensiformis efficiency to reduce Ni content at Sorowako post-mining area.","PeriodicalId":212822,"journal":{"name":"Journal of Microbial Systematics and Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121301775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4