Regulatory Mechanisms in Biosystems最新文献

{"title":"Gender characteristics of lipid metabolism in patients with coronary heart disease and type 2 diabetes mellitus","authors":"Y. S. Yakovleva, T. H. Ostanina, Y. Voronkova, O. Snisar, O. Voronkova","doi":"10.15421/022304","DOIUrl":"https://doi.org/10.15421/022304","url":null,"abstract":"Heart and metabolic diseases are very common in society today. There are many special features in their manifestation that have influence on prognosis and therapy approaches. Among them are gender characteristics. The aim of the research was to determine the gender peculiarities of disorders of lipid metabolism in patients with coronary heart disease and type 2 diabetes mellitus (T2DM). The study of the blood lipid spectrum in patients with coronary heart disease and T2DM was conducted in 72 patients aged 37 to 85 years old. The patients with coronary heart disease were divided into 2 groups depending on the presence or absence of T2DM: 1 group (n = 40) – patients with coronary heart disease only (Stable Angina Pectoris); group 2 (n = 32) – patients with coronary heart disease and T2DM. Each group was divided into subgroups depending on the gender of the patients: 1A and 2A – males, 1B and 2B – females. Despite the presence of T2DM, a majority of patients with coronary heart disease were overweight and obese. At the same time, obesity was more common in the female subgroups (1B – 45.0%, 2B – 64.7%) than in the male subgroups (1A – 35.0% and 2A – 33.3% respectively). There was an increase in the levels of total cholesterol, triglycerides and low-density lipoproteins for impaired lipid metabolism in patients with coronary heart disease. There was a slightly higher level of total cholesterol (6.12 ± 2.06 mmol/L) and low-density lipoproteins (4.24 ± 1.79 mmol/L) and a lower level of triglycerides (1.63 ± 0.65 mmol/L) in females than in males (5.15 ± 1.29, 3.71 ± 1.14 and 2.40 ± 1.06 mmol/l, respectively). Disruption of lipid metabolism was characterized predominantly by an increase in levels of triglycerides and low-density lipoproteins in blood in patients with coronary heart disease and T2DM regardless of gender. About a quarter of males (25.0% with coronary heart disease and 26.7% with coronary heart disease with T2DM) and females with T2DM (23.5%) reached the target total cholesterol level (<4.0 mmol/L), less frequently females with coronary heart disease (15.0%). The incidence of low-density lipoproteins target (<1.8 mmol/L) was significantly lower than total cholesterol: about 5.0% in males (regardless of the presence of T2DM) and 10.0% in females with coronary heart disease. Women with coronary heart disease and T2DM did not reach the target low-density lipoproteins level (<1.8 mmol/L). In most patients with coronary heart disease dyslipidemia grade II by Fredrickson was observed regardless of the presence of T2DM: in 100% of patients with coronary heart disease and 81.1% of patients with coronary heart disease and T2DM. A distinctive feature of impaired lipid metabolism in patients with coronary heart disease and T2DM is the presence in some of patients of Dyslipidemia grade IV by Fredrickson (6.7% of males and 5.9% of females) and the absence of Dyslipidemia class IIa in males in this group. Analysis of the achievement of the target levels o","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43528059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emmer wheat productivity formation depending on pre-sowing seed treatment method in organic and traditional technology cultivation","authors":"I. Korotkova, T. O. Chaika, T. Romashko, O. Chetveryk, A. Rybalchenko, O. Barabolia","doi":"10.15421/022307","DOIUrl":"https://doi.org/10.15421/022307","url":null,"abstract":"Determination of chlorophyll and carotenoid content is an important way of obtaining information about the plant's photosynthetic activity as well as an indirect method of assessing the productivity of plant crops, particularly grain crops. The objective of this study was to evaluate the role of chlorophyll and carotenoid in the productivity formation of emmer wheat (Triticum dicoccum (Schrank.) Schuebl) grown under the traditional and organic farming systems and the different pre-sowing seed treatment methods. The base indicators of the photosynthetic apparatus (chlorophyll and carotenoid content, ratio of photosynthetic pigments) were evaluated in the emmer wheat plants as a function of the cultivation technology and pre-sowing seed treatment. The cultivation of the emmer wheat under organic technology was carried out in crop rotation: winter rye – mustard – T. dicoccum wheat. The pre-sowing seed treatment in the traditional technology of emmer wheat cultivation was carried out only by the UV-C irradiation. In the organic technology, both UV-C irradiation and treatment with humic preparation of natural origin “1r Seed Treatment” were used. The content of chlorophyll a (by 9.2%) and chlorophyll b (by 14.5%) increased in the emmer wheat plants under the organic technology cultivation compared to the traditional technology, but with the same method of seed treatment (UV-C irradiation). As a result, the yield increase was 21.0%. The application of the “1r Seed Treatment” humic preparation in the pre-sowing seed treatment led to the increase in yield by ~ 8.0% compared to the plots with UV-C irradiation seeds treatment under organic farming cultivation. An inverse correlation between the ratio of chlorophyll a/ chlorophyll b and the crop yield has been established. The evaluation of economic indicators of the emmer wheat cultivation in the rotation: winter rye-mustard-emmer wheat under organic farming technology, proved its high profitability. So, the photosynthetic pigments’ content and their ratio can be used as the indicators of the efficiency of the introduced elements of agrotechnologies and for predicting future yields.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46024049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycolysis process activation in preserved red blood cells by nanotechnological treatment of resuspending solutions","authors":"A. Belousov, E. Malygon, T. Kalynychenko, E. Belousova, V. Yavorskiy, M. Anoshyna","doi":"10.15421/022302","DOIUrl":"https://doi.org/10.15421/022302","url":null,"abstract":"Currently, the use of nanotechnology opens up new opportunities to influence the processes of anaerobic glycolysis and the activity of hexose monophosphate reactions in preserved erythrocytes. Components containing donor red blood cells on CPDA-1 preservative were examined. Modified solutions of 0.9% NaCl and with 5% glucose were used as resuspending solutions. The solutions were treated with magnetite nanoparticles (ICNB brand) by the Belousov method. The amounts of 2,3-DPG, ATP, reduced glutathione, and glutathione peroxidase were determined by spectrophotometry. This study opens up new possibilities for increasing the shelf life and functional activity of preserved erythrocytes. The study showed a reliable increase in ATP and reduced glutathione, a decrease in 2,3-DPG and glutathione peroxidase. It was found that the activation of anaerobic glycolysis was less pronounced in tests with modified physiological saline than in tests with glucose solution. On the contrary, the pentose glucose oxidation cycle prevailed. A comprehensive analysis of the data obtained indicates the membrane-protective effect of the modified resuspending solutions. The membrane-protective effect is due to an increase in ATP and reduced glutathione, which ensures the redox potential of the cell in an equilibrium state. Magnetite nanoparticles (ICNB) change the mobility and orientation of hydrogen protons in resuspending solutions. This polarizes the aqueous sector of the erythrocyte microenvironment due to van der Waals forces, which is the main reason for activation of ATP phosphate residue hydrolysis and switching of intracellular enzymes regulating anaerobic glycolysis and pentose phosphate cycle into the active state. As a result, transmembrane metabolism and metabolism change, the energy state of erythrocytes changes, and enzymes are activated. All this has a significant impact on the energy supply of preserved red blood cells and preservation of their functional activity under storage conditions at 2 to 6 ºС.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48250922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The prerequisites for the development of type 2 diabetes or prediabetes in rats fed a high-fat diet","authors":"A. Portnychenko, M. Vasylenko, R. Aliiev, M. G. Kozlovska, M. O. Zavhorodnii, P. Tsapenko, K. Rozova, V. Portnichenko","doi":"10.15421/022303","DOIUrl":"https://doi.org/10.15421/022303","url":null,"abstract":"It is known that the pathogenesis of type 2 diabetes in humans is based on two main factors – insulin resistance and inappropriate secretory activity of β-cells of the pancreas. In animals, the role of these mechanisms has not been clearly characterized, and the differences in the manifestations of experimental diabetes under the same conditions are not sufficiently substantiated. In order to study the prerequisites and mechanisms of the development of experimental type 2 diabetes or prediabetes under lipid overload, 6-month-old male Wistar rats were fed a high-fat diet for 4 weeks; after 2 weeks of the experiment, 20 or 25 mg/kg of streptozotocin was administrated. The development of insulin resistance was assessed using the insulin tolerance test. We evaluated the dynamics of glycemia in animals, subcellular signs of liver steatosis, and determined expression of the precursor and mature protein SREBP-1 by immunoblotting. It was found that in rats fed with a high-fat diet during the 2–4th weeks of the experiment, regardless of the administration of streptozotocin, stable insulin resistance and symptoms of prediabetes were detected. The severity of carbohydrate metabolism lesion, which appeared as type 2 diabetes or prediabetes after streptozotocin administration, depended on the level of hepatosteatosis due to high-fat diet, whereas the dose of streptozotocin influenced severity of type 2 diabetes. The use of a high-fat diet led to increased processing and activation of SREBP-1, which was clearly inhibited in type 2 diabetes. Therefore, the level of lipid infiltration of the liver and deregulation of the transcription factor SREBP-1 are risk factors defining development of type 2 diabetes or prediabetes in experimental rats with lipid overloading. Changes in the maturation of SREBP-1 with the use of a high-fat diet confirm that insulin resistance in rats revealed β-cell dysfunction, which closely approximates the mechanisms of experimental type 2 diabetes to main pathways in humans. At the same time, the predisposition to β-cell dysfunction can be a prerequisite that determines compensatory reserves for maintaining carbohydrate and lipid homeostasis under the influence of lipid load in both humans and laboratory animals.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2022-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47552448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Аntibiotic resistance of bacterial cultures isolated from the feral pigeon (Columba livia) and starling (Sturnus vulgaris) at a solid waste landfill","authors":"Y. Dementieieva, N. Muzyka, D. Muzyka, A. Chaplygina","doi":"10.15421/022258","DOIUrl":"https://doi.org/10.15421/022258","url":null,"abstract":"Resistance to antibiotics is well-known global phenomenon. There are places contributing to the development of antibiotic resistance such as waste landfills, especially ones that accept medical waste which did not undergo disinfection and livestock waste with bacteria not sensitive to antibiotics. An extensive system of transfer of antibiotic resistant microorganisms is formed on these territories (zoochory, groundwater, transport etc.). The aim of the research was to determine the species composition of bacteria isolated from birds of Derhachi municipal solid waste landfills in Kharkiv city, Ukraine. Also, we determine the sensitivity of bacterial isolates to a number of standard antibiotic drugs. We collected droppings of feral pigeons (Columba livia Gmelin, 1789; Columbidae) and starlings (Sturnus vulgaris Linnaeus, 1758; Sturnidae) during the winter period in 2020/2021; both species are dominants of waste landfills. We isolated 15 bacteria species of 4 families by bacteriological methods (growing on simple and selective media and identification by biochemical properties): Enterobacteriaceae (Enterobacter asburiae, E. dissolvens, E. cancerogenus, E. cloacae, E. sakazakii, Escherichia coli, Klebsiella terrigena, K. ornithinolytica, Citrobacter freundii, Proteus mirabilis), Yersiniaceae (Serratia ficaria, S. rubidaea, S. entomophila), Morganellaceae (Providencia stuartii) and Pseudomonadaceaе (Pseudomonas aeruginosa). Sensitivity was determined by the disk-diffusion method to 18 antibiotics. Ten isolates turned out to be multiresistant-resistant to three or more classes of antimicrobial drugs. A promising direction for future research is the determination of the pathogenicity of the isolates and checking the roles of birds of Derhachi solid waste landfills as reservoirs of pathogens. Currently, it can be assumed that large concentrations of synanthropic birds (especially those that forage on solid waste landfills) with a high probability are reservoirs of many bacteria, in particular those that have developed resistance to drugs.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2022-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49599293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Testicular ultrasound examination and hemodynamics of patients with azoospermia","authors":"M. Z. Vorobets, O. Melnyk, R. Fafula, O. K. Onufrovych, A. T. Borzhievsky, Z. Vorobets","doi":"10.15421/022259","DOIUrl":"https://doi.org/10.15421/022259","url":null,"abstract":"The most difficult form of male infertility to treat is azoospermia. Azoospermia is defined as the complete absence of spermatozoa in the ejaculate. The age of the patients who underwent clinical and diagnostic studies varied between 22 and 45 years. Among 119 examined patients with azoospermia, 58.0% were diagnosed with secretory infertility. In 42.0% patients, the presence of spermatogenesis in excretory-obturational infertility was established. Among 69 patients with secretory infertility, 23 had azoospermia in the absence of spermatozoa and spermatogenic cells, which accounted for 33.3% of all patients with secretory infertility (in particular, 2 with leukocytospermia, which indicated damage to the tubular apparatus as a result of previous orchitis). In 66.6% patients, azoospermia was observed in the absence of spermatozoa, but in the presence of precursor cells of spermatogenesis. 11.5% patients out of 69 (group 1) were diagnosed with concomitant diseases. We see that arterial hypertension, diseases of the gastrointestinal tract, liver, and kidneys occur. Hereditary diseases were not detected in the examined patients. Pain of varying intensity was found in 6.7% of patients, varying degrees of testicular hypoplasia were found in 7.6%, dysuria in 13.4%, epididymitis in history 8.4%; history of epidemic mumps 6.7%; 12.6% had depression, restlessness, sleep disorders, and 21.0% had erectile dysfunction. According to clinical examination and ultrasound, chronic prostatitis is suspected in 19.2% of patients. An increase in the number of leukocytes in the blood was found in 13.2% patients. According to ultrasound, 60.5% of the patients with azoospermia had normal testicular sizes, and 39.5% had reduced testes. The volume of the testicles in the control group was 22.3 ± 2.1 cm³ on average, varying from 18.3 to 25.1 cm³. In the group with azoospermia, the volume of the testicles was on average 16.7 ± 1.7 cm³ and varied from 8.2 to 21.1 cm³, that is, the volume of the testicles in patients with azoospermia was on average 1.3 times smaller compared to normozoospermia. In the obstructive form of azoospermia, diffuse changes were detected in both testicles, probably obturational changes. Hypoplasia of the left testicle and increased echogenicity were observed in the non-obstructive form of azoospermia. The veins of the spermatic cord were moderately dilated. There was a normal amount of free fluid in the scrotum. According to elastography, the elasticity of the testicles was above normal. Obturational processes in the testicles were suspected. The hemodynamic parameters of testicular parenchymal blood flow in infertile men obtained by ultrasound dopplerography are of important diagnostic value. The average value of the linear blood flow velocity in the arteries of the parenchyma in men with normozoospermia was 0.107 ± 0.015 m/s on the right, and 0.103 ± 0.012 m/s on the left. With azoospermia, the average value of the linear velocity of blood flow on the","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2022-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44885793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patho-histological features of fibroblastic sarcoid in horses","authors":"M. Portenko, O. Shchebentovska","doi":"10.15421/022252","DOIUrl":"https://doi.org/10.15421/022252","url":null,"abstract":"Sarcoid is one of the most common skin tumours, which is diagnosed in various breeds of horses at any age regardless of the animal’s sex. Such fibroblastic neoplasm manifests itself in several clinical ways with its specific morphological features. As a rule, it metastasizes rarely but can be locally invasive. The disease’setiology is multifactorial and includes the confirmed role of the bovine papillomavirus and genetic and environmental factors. The article introduces the results of a retrospective study of horses of the Ukrainian riding breed with a fibroblastic sarcoid type, which was localized mainly in the area of the external thoracic vein. Neoplasms were represented by two types. The first one included broad base neoplasms without a pronounced pedicle but with signs of hyperkeratosis. Massive rounded dense formations with an ulcerated surface refer to the second type. To verify the studied types of sarcoid, wide surgical excision of the lesion’svisible parts was performed, followed by the selection of fragments for the pathohistological diagnosis. Also, additional staining methods were used to detect the connective and muscle tissue (Van Gieson’s staining) and elastic fibers (Weigert’s resorcin-fuchsin staining). Microscopically, the neoplasm without a visible pedicle showed signs of hyperkeratosis and acanthosis in the peritumoral epidermal layer. Epidermis desquamation with a significant amount of oxyphilic detritus, diapedesis hemorrhages, and dilated vessels was also detected in some areas. Chaotic placement of fibroblasts and fibrocytes was noted, to a greater extent, subdermally. Anisocytosis and anisokaryosis were expressed moderately. Sarcoids, macroscopically presented in the form of massive dense formations with an ulcerated surface, were histologically characterized by the replacement of the epidermis with cellular elements, subdermal hemorrhages, and the characteristic perpendicular orientation of coarse collagen fibers towards the basement membrane. Closely spaced spindle-shaped cells with fibers forming moiré structures were visualized in the deep dermis layers. Nuclei were hyperchromic and elongated in shape, no atypical mitoses were noted. Pathohistological examination during the identification of a sarcoid type for diagnostic purposes can cause rapid growth and formation of an ulcer. The final diagnosis regarding equine sarcoid can be made based on a combination of clinical signs, histopathological examination, and molecular genetic testing with the isolation of BPV DNA.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2022-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45757856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrogen-fixing, phosphate-potassium-mobilizing ability of Rahnella bacteria isolated from wheat roots","authors":"Z. Shakirov, K. Mamanazarova, I. Yakubov, S. Zakiryaeva, K. Khamidova","doi":"10.15421/022250","DOIUrl":"https://doi.org/10.15421/022250","url":null,"abstract":"As the number of people on earth increases, so does the need for food. Providing the population with environmentally friendly agricultural food is one of the urgent problems of our time. Currently, the main direction of modern organic farming is the use of biofertilizers. Bacterial preparations are capable of influencing the physiological processes of plants in small quantities, leading to increase in plant productivity. The objective of this work was to study rhizobacteria associated with wheat roots. For this purpose, we took more than 100 isolates of rhizobacteria from the rhizosphere and root surface of wheat plants grown in irrigated fields of Tashkent, Syrdarya, Andijan, Kashkadarya regions. Rhizobacteria were grown on nutrient media of Döbereiner, Ashby, Pikovsky, and Zack, and 25 isolates of associative rhizobacteria were selected based on the characteristics of absorption of molecular nitrogen, mobilization of phosphorus and potassium. They actively dissolved Сa3(PO4)2 and KAlSiO4 for 3 days. They were found to produce organic acids. In organic farming, nitrogen-fixing, phosphorus- and potassium-mobilizing rhizobacteria are of great practical importance, while our experiments on obtaining biological products are considered as an environmentally friendly and cost-effective way to increase crop yields. From the surface of wheat roots grown in different zones of Uzbekistan, when screening for nitrogen fixation, we selected 3 isolates with acetylene reductase activity of 79–91 nmol C2H4/flacon/24h. We determined that bacteria completely mobilized phosphate, forming 100% acid when grown in a medium containing Ca3(PO4)2 for 5 days. The ability of the bacteria to mobilize potassium was studied on a nutrient KAlSiO4-containing medium. The bacteria were observed to mobilize potassium, forming 90–100% acid within 15 days. Based on the study of the 16S rRNA gene of bacteria, we identified rhizobacteria UT3, UT4, and UT9 as Rahnella aquatilis.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2022-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42587298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioclimatic projection of the ecological niche of curly mallow (Malva verticillata) based on the forecast of the dynamics of the geographical range in the context of global climate change","authors":"K. Panchenko","doi":"10.15421/022253","DOIUrl":"https://doi.org/10.15421/022253","url":null,"abstract":"Curly mallow (Malva verticillata L.) is a promising species for cultivation to obtain valuable compounds for the application in medicine, and this species can be used in the bioenergy system to provide industry with alternative energy sources. For the highest economic efficiency, the practical use of this species requires the development of complex measures related to both agrotechnologies and selective breeding. Such measures require resources and there is an urgent problem of assessing the prospects of such investments taking into account the global climate change. Therefore, the problem that we aimed to solve was the assessment of how the global climate change would impact the curly mallow in general in the global context, as well as in the conditions of Ukraine in the next 50–70 years. The database of the Global Biodiversity Information Facility (GBIF) contains 2,104 records of curly-leaved mallow. This species is found on all the continents except Antarctica. Asia accounts for 39.1% of the species’ range, Europe – 53.3%, Africa – 3.6%, North America – 3.2%, South America – 0.1%, Australia – 0.8%. The modelling of M. verticillata response to the climatic factors showed that the best response models were V (in 31.6% of cases) and VII (in 36.8% of cases). Model V characterizes unimodal bell-shaped asymmetric response, and model VII – bimodal asymmetric response. The species response to the mean annual temperature is asymmetric bell-shaped with a shift to the right. The optimal average annual temperature for this species is 9.1 °C. Comparing the distribution of available resources and their use is the basis for identifying the features of the ecological niche of the species. The MaxEnt approach indicates that Southeast Asia and Europe have the most favourable conditions for the existence of this species. Changes in the climatic conditions over the next 50–70 years will make the conditions for the life of M. verticillata in the southern hemisphere unfavourable, and the favourable conditions for it in the northern hemisphere will shift significantly to the north. At the same time, conditions in the autochthonous range of the species will become unfavourable. Obviously, if not for the significant potential of the species to disperse, it would have died out as a result of the significant climate change. The area where favourable conditions for the species will remain unchanged is Central Europe. Conditions in Eastern Europe, including Ukraine, will moderately improve. The results indicate the perspective of the cultivation of curly mallow in Ukraine in the future.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2022-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47232335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low doses of imidacloprid induce neurotoxic effects in adult marsh frogs: GFAP, NfL, and angiostatin as biomarkers","authors":"S. Yermolenko, V. Nedzvetsky, V. Gasso, V. A. Spirina, V. Petrushevskyi, V. Kyrychenko","doi":"10.15421/022256","DOIUrl":"https://doi.org/10.15421/022256","url":null,"abstract":"Imidacloprid is one of the most widely used insecticides in the world. The neurotoxicity of imidacloprid in adult amphibians has not been studied thoroughly. We investigated the expression of glial fibrillary acidic protein (GFAP), neurofilament light chain (NfL) and angiostatin in the amphibian brain to identify valid biomarkers of low dose imidacloprid exposure. For the experiment, 30 individuals of the marsh frog Pelophylax ridibundus were selected. The amphibians were divided into five groups. The duration of the experiment was 7 and 21 days. The exposure concentrations were 10 and 100 µg/L. The results of the study revealed a decrease in the expression of GFAP after 7 days in the exposure groups of 10 and 100 μg/L. An increase in the level of NfL was observed in the group exposed to 10 μg/L after 21 days of the experiment. The angiostatin level was increased after 7 days at 10 µg/L and after 21 days at 100 µg/L. The data obtained indicate that low concentrations of imidacloprid can cause neurotoxic effects in the brain of P. ridibundus. Such effects can have a significant impact on amphibian populations. According to the results of the study of the expression level of GFAP, NfL and angiostatin, it can be stated that imidacloprid has a neurotoxic effect on adult marsh frogs. The studied indicators can be promising biomarkers of environmental pollution by neonicotinoids.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48606834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}