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Evaluation of bacterial quality of Oreochromis niloticus samples of Lake Manzala, Egypt 埃及曼萨拉湖尼罗褐口鱼样品细菌质量评价

Alfarama Journal of Basic & Applied Sciences Pub Date : 2020-06-07 DOI: 10.21608/ajbas.2020.23319.1009

M. El-Ghannam, A. Hanora, H. Abdulla, M. Zaky

{"title":"Evaluation of bacterial quality of Oreochromis niloticus samples of Lake Manzala, Egypt","authors":"M. El-Ghannam, A. Hanora, H. Abdulla, M. Zaky","doi":"10.21608/ajbas.2020.23319.1009","DOIUrl":"https://doi.org/10.21608/ajbas.2020.23319.1009","url":null,"abstract":"The present study aimed to investigate bacterial quality of tilapia fish (Oreochromis niloticus) and evaluate the hygienic health hazard of fish contaminated with some food borne pathogens. Bacteria were isolated from intestine, gills and flesh of Oreochromis niloticus collected from Lake Manzala during two seasons; winter and summer, 2017. Microbiological studies was applied for all samples as total viable bacteria, total coliform, faecal coliform, faecal streptococcus and examined for the presence of Pseudomonas sp. Results show that total viable bacterial count mean was highest in intestine 222 x 104 CFUg-1 in summer. Total coliform count mean was highest in intestine 425 x 103 CFUg-1 in summer. Faecal coliform count mean was highest in gills 90 x 102 CFUg-1 in summer. Faecal streptococcus detected in fish organs only in summer. P. aeruginosa count mean was highest in intestine 185 x 102 CFUg-1 in summer. Molecular identification of suspected colonies was as DNA extraction, PCR technique using ISSR protocol and sequencing, using the nucleotide sequences of the genes for 16S rRNA revealed different strains of Pseudomonas aeruginosa. All isolates were tested for resistance to 10 groups of antibiotics namely Tetracycline (10μg), Gentamicin (10μg), Ampicillin (10μg), Trimethoprim/Sulphamethoxazole (25μg), Vancomycin (30μg), Erythromycin (15μg), Ciprofloxacin (30μg), Chloramphenicol (30μg), Amoxicillin (10μg) and Rifampicin (5μg) using Kirby-Bauer disc diffusion method. The results showed high frequency of multi-drug resistance to many antibiotics, particularly penicillin, ampicillin and chloramphenicol. Therefore this fish species caught from the Lake Manzala may pose health hazards to human and the whole ecosystem.","PeriodicalId":210712,"journal":{"name":"Alfarama Journal of Basic & Applied Sciences","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130925092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Solvation Thermodynamic Parameters for Cocamidopropyl Betaine (CAPB) Surfactant in Aqueous and Alcoholic-Aqueous Solvents 椰油酰胺丙基甜菜碱(CAPB)表面活性剂在水溶液和醇-水溶液中的溶剂化热力学参数

Alfarama Journal of Basic & Applied Sciences Pub Date : 2019-09-24 DOI: 10.21608/ajbas.2019.17224.1001

F. El-Dossoki

引用次数: 0

In vitro efficacy of biologically active compounds derived from Navicula arenaria against soil borne phytopathogenic Macrophomina phaseolina and Fusarium oxysporum 砂沙Navicula arenaria生物活性化合物对土源植物病原菌phaseolina Macrophomina和oxysporum的体外药效研究

Alfarama Journal of Basic & Applied Sciences Pub Date : 1900-01-01 DOI: 10.21608/ajbas.2021.67687.1048

Asmaa L. Alallaf, Metwally Kotab, H. Shafik, A. Elsayed

{"title":"In vitro efficacy of biologically active compounds derived from Navicula arenaria against soil borne phytopathogenic Macrophomina phaseolina and Fusarium oxysporum","authors":"Asmaa L. Alallaf, Metwally Kotab, H. Shafik, A. Elsayed","doi":"10.21608/ajbas.2021.67687.1048","DOIUrl":"https://doi.org/10.21608/ajbas.2021.67687.1048","url":null,"abstract":"Navicula arenaria Donkin 1861 is a marine microalga belongs to class of bacillariophycea. Recently, microalgae and their products are used as biological control of diseases caused by phytopathogenic fungi. This is considered environmentally ecofriendly method to overcome the plant damage caused by soil borne pathogenic fungi and thereby economic loss. Therefore, this study aimed to investigate in vitro the antifungal activity of N. arenaria isolate PS 31 extracellular and intracellular metabolites against two taxa of soil borne phytopathogenic fungi; Macrophomina phaseolina and Fusarium oxysporum. N. arenaria PS 31 hexane extract was the most effective extract on growth inhibition of both phytopathogenic fungi. There is no significant difference between miconazole and N. arenaria hexane extract of 5.6 mg/ml on growth inhibition of F. oxysporum. The inhibitory effect of hexane extract at 5.6, 4.2 and 2.8 mg/ml and ethyl acetate extract at 35% (v/v) was higher than controls. Ethyl acetate extract was effective on growth inhibition of M. phaseolina (29.67%). GC-MS analysis of N. arenaria hexane fraction revealed the presence of potent antifungal compounds such as Phenol, 2,2'-methylenebis[6-(1,1-dimethylethyl)-4methyl-, di-n-octyl phthalate, cholestane-3,5-diol, 5-acetate,(3.beta.,5.alpha.), Cholestan-3ol,(3.beta.,5.beta.)and beta.-Sitosterol. These results suggest that N. arenaria hexane extract can be used in biological control of plant diseases caused by M. phaseolina and F. oxysporum.","PeriodicalId":210712,"journal":{"name":"Alfarama Journal of Basic & Applied Sciences","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115662008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

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