Research communications in molecular pathology and pharmacology最新文献_第9页

Genistein, a soy isoflavone, enhances necrotic-like cell death in a breast cancer cell treated with a chemotherapeutic agent. 染料木黄酮，一种大豆异黄酮，在化疗药物治疗的乳腺癌细胞中促进坏死样细胞死亡。

Research communications in molecular pathology and pharmacology Pub Date : 2003-01-01

Haruna Satoh, Kazuhiro Nishikawa, Kazuyuki Suzuki, Ryuji Asano, Nantiga Virgona, Tomio Ichikawa, Kiyokazu Hagiwara, Tomohiro Yano

引用次数: 0

Modulation of articular chondrocyte activity by pirfenidone. 吡非尼酮对关节软骨细胞活性的调节。

Research communications in molecular pathology and pharmacology Pub Date : 2003-01-01

Hilary P Benton, Angelica V Esquivel, Amber D Rice, Shri N Giri

{"title":"Modulation of articular chondrocyte activity by pirfenidone.","authors":"Hilary P Benton, Angelica V Esquivel, Amber D Rice, Shri N Giri","doi":"","DOIUrl":"","url":null,"abstract":"Pirfenidone is under investigation as an anti-inflammatory and anti-fibrotic agent in several organs including lung. Since important features of arthritic conditions include inflammation and long-term damage to articular cartilage, we have investigated whether PD can suppress chondrocyte responses to bacterial lipopolysaccharide (LPS) and interleukin 1 (IL-1); modulators that induce a cascade of inflammatory responses that lead to articular joint tissue damage. PD (0 - 5microM) showed no effect on cell number or viability when incubated with high density primary equine chondrocyte cultures for a 24 hr period. PD did not stimulate nitric oxide (NO) release by chondrocytes when added alone but LPS and IL-1-induced NO release was inhibited by PD, in a dose-dependent manner. PD did not significantly influence GAG release from cartilage matrix nor did it stimulate or suppress the GAG releasing actions of LPS or IL-1. We conclude that PD is capable of attenuating the cytokine-induced production of the inflammatory mediator, NO by chondrocytes, without stimulating matrix glycosaminoglycan loss from cartilage. PD may have potential as an anti-inflammatory agent in the joint.","PeriodicalId":21045,"journal":{"name":"Research communications in molecular pathology and pharmacology","volume":"113-114 ","pages":"275-88"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24936307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accelerated acetylcholine metabolism in the lung after infusion of phenylephrine in dogs. 注射苯肾上腺素后狗肺内乙酰胆碱代谢加速。

Research communications in molecular pathology and pharmacology Pub Date : 2003-01-01

Tsutomu Nakahara, Hiroaki Nejishima, Koichi Nakayama, Kunio Ishii

引用次数: 0

Fructose diphosphate attenuates the acetaminophen-induced liver injury in the rat evidence for involvement of nitric oxide. 果糖二磷酸减轻了对乙酰氨基酚引起的大鼠肝损伤，证明了一氧化氮的参与。

Research communications in molecular pathology and pharmacology Pub Date : 2003-01-01

Anastasios A Mihas, Vijaya K Kanji, Thanos A Mihas, Roy M Joseph, Angel K Markov, Douglas M Heuman

{"title":"Fructose diphosphate attenuates the acetaminophen-induced liver injury in the rat evidence for involvement of nitric oxide.","authors":"Anastasios A Mihas, Vijaya K Kanji, Thanos A Mihas, Roy M Joseph, Angel K Markov, Douglas M Heuman","doi":"","DOIUrl":"","url":null,"abstract":"We have previously shown that fructose-1,6-diphosphate (FDP) stimulates the synthesis of nitric oxide probably by stimulating the hepatic inducible nitric oxide synthase (iNOS). The aim of the present study was to evaluate the hepatoprotective role of FDP in acetaminophen-induced liver injury and whether this hepatoprotective effect is mediated by nitric oxide. Liver injury was induced in adult Sprague-Dawley rats by the administration of acetaminophen (1.6 g/kg by gavage) 10 min prior to the intraperitoneal injection of either FDP or normal saline. Liver injury was assessed by alanine aminotransferase (ALT) activity in the serum. iNOS and malondialdehyde (MDA) levels were determined in liver homogenates. Acetaminophen produced striking elevations of serum ALT, high MDA levels and a profound decrease in the liver iNOS. Administration of FDP attenuated the ALT and MDA elevations and prevented the liver iNOS depletion caused by acetaminophen. Pretreatment of the animals with the iNOS inhibitor L-NAME abolished this hepatoprotection. These findings suggest that FDP protects against acetaminophen-induced liver injury, at least partly, by stimulating production of nitric oxide.","PeriodicalId":21045,"journal":{"name":"Research communications in molecular pathology and pharmacology","volume":"113-114 ","pages":"253-66"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24936361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

N3-phenacyluridine as a new type of antinociceptive compound in mice. N3-phenacyluridine是一种新型的小鼠抗伤害性化合物。

Research communications in molecular pathology and pharmacology Pub Date : 2003-01-01

Toshiyuki Kimura, Tomomi Shimizu, Tatsuya Funahashi, Mayumi Nagakura, Kazuhito Watanabe, Kuniomi Tachibana, Shigemi Kondo, Ing Kang Ho, Ikuo Yamamoto

{"title":"N3-phenacyluridine as a new type of antinociceptive compound in mice.","authors":"Toshiyuki Kimura, Tomomi Shimizu, Tatsuya Funahashi, Mayumi Nagakura, Kazuhito Watanabe, Kuniomi Tachibana, Shigemi Kondo, Ing Kang Ho, Ikuo Yamamoto","doi":"","DOIUrl":"","url":null,"abstract":"The antinociceptive activity of N3-phenacyluridine, a novel hypnotic, was examined with tail pinch, hot plate and acetic acid-induced abdominal constriction methods by intracerebroventricular (i.c.v.) injection to mice. In the first method, N3-phenacyluridine exerted antinociceptive activity after the i.c.v. injection at a dose of 0.5 micromol/mouse, although this activity was comparable to the activity of morphine (0.01 micromol/mouse, i.c.v.). In the second method, the activity of N3 -phenacyluridine was continued until 120 min after the injection at a dose of 0.5 micromol/mouse. In last, N3-phenacyluridine (0.1 micromol/mouse, i.c.v.) significantly decreased in the numbers of acetic acid-induced abdominal constriction as compared to the control (1% Tween 80 saline). The ED50 value of the antinociceptive activity of N3-phenacyluridine was 0.02 imol/mouse, i.c.v. The present paper demonstrated for the first time that N3-phenacyluridine belonging to oxopyrimidine nucleoside analogue possesses not only the hypnotic and sedative activities previously reported, but also antinociceptive activity.","PeriodicalId":21045,"journal":{"name":"Research communications in molecular pathology and pharmacology","volume":"113-114 ","pages":"57-65"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24936519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced expression of PP1gamma1, a catalytic subunit isoform of protein phosphatase type1 and expression of telomerase activity in Ewing's sarcoma cells. 尤因肉瘤细胞中PP1gamma1(1型蛋白磷酸酶的催化亚基异构体)的表达和端粒酶活性的表达增强。

Research communications in molecular pathology and pharmacology Pub Date : 2003-01-01

Satoshi Mori, Yongping Cao, Takahisa Yamadab, Kenichi Sogawa, Kazuya Kondo, Norifumi Hino, Tatsuhiko Miyazaki, Yoji Kawaguchi, Shiro Oka, Kojiro Kawasaki, Tasuku Mashiba, Hiromichi Norimatsu

引用次数: 0

Fas/Fas-ligand expressions in peripheral-blood mononuclear cells of patients with myelodysplastic syndromes. 骨髓增生异常综合征患者外周血单个核细胞中Fas/Fas配体的表达

Research communications in molecular pathology and pharmacology Pub Date : 2003-01-01

Toshihiko Hirano, Noriko Hiratsuka, Tohru Iwahori, Kitaro Oka, Kazunori Wakasugi

{"title":"Fas/Fas-ligand expressions in peripheral-blood mononuclear cells of patients with myelodysplastic syndromes.","authors":"Toshihiko Hirano, Noriko Hiratsuka, Tohru Iwahori, Kitaro Oka, Kazunori Wakasugi","doi":"","DOIUrl":"","url":null,"abstract":"Increased expressions of Fas and Fas-ligand (Fas-L) in bone marrow cells of myelodysplastic syndromes (MDS) have been reported, and large number of these \"cell-death signals\" might explain molecular basis for exacerbation of apoptosis in marrow cells of these syndromes. However, expression of these molecules or progression of apoptosis in peripheral-blood mononuclear cells (PBMCs) in MDS has little been investigated. In the present study, we compared expression of these cell-death molecules and percentages of apoptotic cells in PBMCs between MDS patients and healthy subjects. PBMCs were obtained from 7 MDS patients and 8 age-matched healthy controls. Five out of 7 patients were MDS with refractory anemia (RA) type, while the other 2 were MDS-RA with excess of blasts (MDS-RAEB) type. Percentages of PBMCs expressing Fas, Fas-L, and phosphatidylserine as a cell apoptosis-marker were determined by staining cells with FITC-labeled anti-CD95 (Fas) antibody, biotinyl anti Fas-L antibody, and annexin V, respectively. The cells were subsequently analyzed with flow cytometry. The mean (SD) percentage of Fas-expressing PBMCs in MDS group was 55.3 (13.9), whereas the value in healthy subjects was 30.6 (8.8) %, and thus the ratio of Fas positive cells in PBMCs of MDS was significantly higher than that of healthy subjects (p < 0.002). In contrast, the mean (SD) of Fas-L expressing PBMCs in MDS (n=5) was 18.4 (12.2) %, which was significantly lower (p < 0.02) than that in healthy subjects (34.4 +/- 8.1%; n=7). The mean (SD) of apoptotic PBMCs detected as annexin V-positive, non-necrotic cells in MDS (n=7) was 23.3 (7.5) %, which was not significantly different from that in healthy subjects (22.2 +/- 7.8%; n=8). Thus, PBMCs in MDS express high levels of Fas, whereas they conversely exhibit low levels of Fas-L, which may result in prevention of apoptosis by the death signals, and in cell-survival in these cells.","PeriodicalId":21045,"journal":{"name":"Research communications in molecular pathology and pharmacology","volume":"113-114 ","pages":"315-28"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24936311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The relationship between oxidative metabolism and lipid peroxidation in rat liver microsomes. 大鼠肝微粒体氧化代谢与脂质过氧化的关系。

Research communications in molecular pathology and pharmacology Pub Date : 2003-01-01

Bin Ji, Y Masubuchi, T Horie

引用次数: 0

Effects of acute renal failure induced by uranyl nitrate on the pharmacokinetics of intravenous torasemide in rats. 硝酸铀酰致急性肾功能衰竭对静脉注射托拉塞米药动学的影响。

Research communications in molecular pathology and pharmacology Pub Date : 2003-01-01

Ae K Lee, Eun J Kim, Myung G Lee

引用次数: 0

Comparison of different de-endothelization procedures in the isolated rat thoracic aorta: a short communication. 大鼠离体胸主动脉不同去内皮化方法的比较。

Research communications in molecular pathology and pharmacology Pub Date : 2003-01-01

C Uluoglu, H Zengil

{"title":"Comparison of different de-endothelization procedures in the isolated rat thoracic aorta: a short communication.","authors":"C Uluoglu, H Zengil","doi":"","DOIUrl":"","url":null,"abstract":"The aim of this study was to compare the effects of different techniques used for deendothelization on responses to endothelium-dependent (acetylcholine) and -independent (sodium nitroprusside) vasodilators and vasoconstrictor (phenylephrine) in the isolated rat thoracic aorta. Four different methods were used for de-endothelization: (1) mechanical (wire), (2) physical (distilled water), (3) chemical (Triton X-100 and saponin) and (4) enzymatic (trypsin). The results showed that mechanical rubbing with rough-surfaced wire was the most effective procedure amongst five different techniques used for deendothelization in the isolated rat thoracic rings. Denudation by wire abolished endothelium-dependent relaxation successfully without much interfering smooth muscle vasodilating and contracting properties of the agonists. It seems that physical, chemical and enzymatic techniques are not suitable for endothelial denudation in the isolated thoracic rat aorta preparations. These endothelium removing methods may be useful for studying the role of endothelium in vascular beds or small diameter vessels whose endothelial cells can not be removed mechanically.","PeriodicalId":21045,"journal":{"name":"Research communications in molecular pathology and pharmacology","volume":"113-114 ","pages":"289-97"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24936308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0