Research on Crops最新文献_第3页

Optimizing bioherbicide application timing and plant spacing to manage weed growth in rice (Oryza sativa L.) cultivation 优化生物除草剂的施用时间和株距，管理水稻（Oryza sativa L.）种植中的杂草生长

Research on Crops Pub Date : 2024-05-22 DOI: 10.31830/2348-7542.2024.roc-1057

Paiman, Zulkaisi Nurul Amar, Ikhsan Firmansyah, Hageng Rahmatulloh Priyo Pambudi, Anggunila, C. T. Kusumastuti, Ardiyanta

{"title":"Optimizing bioherbicide application timing and plant spacing to manage weed growth in rice (Oryza sativa L.) cultivation ","authors":"Paiman, Zulkaisi Nurul Amar, Ikhsan Firmansyah, Hageng Rahmatulloh Priyo Pambudi, Anggunila, C. T. Kusumastuti, Ardiyanta","doi":"10.31830/2348-7542.2024.roc-1057","DOIUrl":"https://doi.org/10.31830/2348-7542.2024.roc-1057","url":null,"abstract":"Weed management is crucial for successful rice cultivation. Bioherbicides derived from cogon grass extract offer a promising solution for weed control. Additionally, optimizing plant spacing has been recognized as an effective method to suppress weed growth. Thus, this study aims to investigate the impact of cogon grass extract application timing and plant spacing on weed growth in rice cultivation. The study was conducted from October 2022 to February 2023 in the Special Region of Yogyakarta, Indonesia. The study was 3 × 2 factorial and arranged in a randomized completely block design (RCBD). The first factor was the bioherbicide application timing: control (no application), three days before planting, and at the time of planting. The second factor was plant spacing: 15 × 15 and 25 × 25 cm. In total, there were six treatment combinations and three replications (replications as blocks). The research results indicated the application timing of bioherbicide did not significant effect on the growth of weed and rice, as well as the grain dry weight. Fimbristylis miliacea was obtained as the dominant weed species. Bioherbicide application at the time of planting could alter the composition of weed species compared to the control in plant spacing of 15 × 15 cm. Furthermore, the plant spacing of 25 × 25 cm resulted in higher grain and weed dry weight (19.40 g/clump and 45.00 g/0.25 m2) compared to 15 × 15 cm (12.92 g/clump and 23.04 g/0.25 m2). On the contrary, the grain dry weight per hectare was higher in 15 × 15 cm (5.74 t/ha) than in 25 × 25 cm (3.10 t/ha). The research findings indicate that closer plant spacing can suppress weed growth and increase the grain dry weight per hectare compared to wider plant spacing. We recommend that the use of cogon grass extract dosage needs to be increased above 50 L/ha.\u0000","PeriodicalId":21022,"journal":{"name":"Research on Crops","volume":"67 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141110080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing the efficacy of biochar derived from crop residues on rice (Oryza sativa L.) dry matter, yield accumulation and soil organic carbon in Central Vietnam 提高作物秸秆生物炭对越南中部水稻（Oryza sativa L.）干物质、产量积累和土壤有机碳的功效

Research on Crops Pub Date : 2024-05-22 DOI: 10.31830/2348-7542.2024.roc-1060

T. D. H. Truong, T. D. TRAN, Q. C. Nguyen, T. T. Nguyen, T. Hoang

{"title":"Enhancing the efficacy of biochar derived from crop residues on rice (Oryza sativa L.) dry matter, yield accumulation and soil organic carbon in Central Vietnam ","authors":"T. D. H. Truong, T. D. TRAN, Q. C. Nguyen, T. T. Nguyen, T. Hoang","doi":"10.31830/2348-7542.2024.roc-1060","DOIUrl":"https://doi.org/10.31830/2348-7542.2024.roc-1060","url":null,"abstract":"Biochar from crop residues plays an important role in reclamation of degraded rice soil due to imbalance fertilizer application and soil carbon sequestration. The objective of this study aimed to evaluate the best type and rate of biochar application for rice dry matter and soil organic carbon improvement. Therefore, pot experiments were carried out in two seasons of spring and summer 2023 at the University of Agriculture and Forestry, Hue University. Twelve treatments including 2 types of biochar (rice husk and peanut shell) and 6 application rates (0, 1, 2, 3, 4, 5%) were used and arranged in a randomized block design with three replications. Research results showed that the shoot dry matter was higher 9.5 – 27.0% (spring season) and 19.1 – 26.9% (summer season) at a rate of 5% application in both types of biochar than control. Rice husk biochar application had higher shoot dry matter than peanut shell biochar application in the same application rate from 0.30 – 0.46 g/plant in both seasons. Similarly, organic carbon was found the highest content (2.15 - 2.21%) when the application of biochar from crop residues at a rate of 5%. Biochar from crop residues is considered as a good amendment for increasing crop dry matter and soil organic carbon.\u0000","PeriodicalId":21022,"journal":{"name":"Research on Crops","volume":"57 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141108518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental surveillance of the rice massif in the Kuban-Protoka river region 库班-普罗托卡河地区水稻丘陵的环境监测

Research on Crops Pub Date : 2024-05-22 DOI: 10.31830/2348-7542.2024.roc-1083

Victoria Vladimirovna Ustinova, Alexey Dmitrievich Timoshkin

{"title":"Environmental surveillance of the rice massif in the Kuban-Protoka river region ","authors":"Victoria Vladimirovna Ustinova, Alexey Dmitrievich Timoshkin","doi":"10.31830/2348-7542.2024.roc-1083","DOIUrl":"https://doi.org/10.31830/2348-7542.2024.roc-1083","url":null,"abstract":"The article presents the results of environmental monitoring of the rice massif between the Kuban-Protoka rivers. The analysis of the dynamics of the yield of rice crops grown in the zone of risky agriculture, namely in the territory subject to secondary salinization, is carried out. Based on the results of chemical analyses of water and soil, methods of increasing fertility on degraded lands are proposed. In the summer and autumn of 2023, field visits were made to the territory of the Petrovsko-Anastasievskaya, Chernoerkovskaya, Azov and Temryuk irrigation systems in order to visually inspect them and take samples of water and soil for subsequent laboratory chemical analysis. Field visits were carried out during the beginning of the growing season and during the formation of the final drainage and discharge runoff, in order to track the dynamics of changes in the quality indicators of irrigation, waste water and soil. As a result of the conducted research, it was found that the quality of irrigation waters in the studied irrigation systems does not comply with irrigation standards. Chemical analysis of the soil from rice checks showed an excess of maximum permissible concentration (MPC) values of Cl-, SO42-, Na+ и NH4+ ions in the studied irrigation systems. Such results are facilitated by the spread of secondary salinization, since the Kuban River Delta is a modern accumulation of salts from the entire river basin. Soil salinization has a negative impact on agricultural production. Thus, with the help of timely monitoring of saline lands, it is possible to prevent degradation processes using effective melioration and reclamation methods.","PeriodicalId":21022,"journal":{"name":"Research on Crops","volume":"43 23","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141108844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of establishment methods and seed coating techniques on the performance of direct seeded rice (Oryza sativa L.) under Periyar Vaigai command area of Tamil Nadu 泰米尔纳德邦 Periyar Vaigai 指挥区直播稻（Oryza sativa L. ）的种植方法和种子包衣技术对其产量的影响

Research on Crops Pub Date : 2024-05-22 DOI: 10.31830/2348-7542.2024.roc-1074

M. Jayaraj, R. D. Singh, T. Ragavan, S. A. H. Hussainy, P. Subramanian, B. Saliha, B. Sivasankari

{"title":"Influence of establishment methods and seed coating techniques on the performance of direct seeded rice (Oryza sativa L.) under Periyar Vaigai command area of Tamil Nadu","authors":"M. Jayaraj, R. D. Singh, T. Ragavan, S. A. H. Hussainy, P. Subramanian, B. Saliha, B. Sivasankari","doi":"10.31830/2348-7542.2024.roc-1074","DOIUrl":"https://doi.org/10.31830/2348-7542.2024.roc-1074","url":null,"abstract":"Rice is a crucial staple crop cultivated extensively in command areas worldwide. In the command areas, the release of water varies due to the inadequate amount of rainfall in the source area and its proper supply. To combat such situations, direct seeding (DS) methods can be opted for; however, lacunae like excess seeds per drop, scattering of seeds, and intercultural operations like thinning, gap filling, and excess weed growth need a clear refinement to enhance the profitability of rice cultivation. Therefore, a research trial was conducted during 2022-24 at Central Farm, Agricultural College and Research Institute, Madurai, to evolve a suitable direct seeding method. The present investigation was laid out under a split-plot design comprising four main plot treatments and four subplot treatments replicated thrice. The main plot treatments comprised different seeding methods (S) and the subplot treatments comprised different seed coating techniques (P). Based on the experimental results, paper roll seeding and DS + Seed coating @ 2% KNO3 fb Bacillus sp. and Azospirillum (PR + P-KN) combination performed better on establishment with grain (6150 kg/ha) and straw yield (7824 kg/ha) respectively. Regarding the B:C ratio, OS-GP + P-KN resulted in higher with 2.64, closely followed by PR + P-KN with 2.61. The least effect was recorded in the combination of round slots in rice drum seeder and dry seeding (RS + DS). Investigating different techniques of direct seeding and seed coating is essential across various regions, particularly in regulated areas where water discharge remains unpredictable.\u0000","PeriodicalId":21022,"journal":{"name":"Research on Crops","volume":"54 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141113335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of broad-spectrum herbicide mixtures on weed flora in wet direct seeded rice (Oryza sativa L.) in the east coast plain region of India 广谱除草剂混合物对印度东海岸平原地区湿直播稻（Oryza sativa L.）杂草群的功效

Research on Crops Pub Date : 2024-05-22 DOI: 10.31830/2348-7542.2024.roc-1076

S. Meher, Sanjoy Saha, Nitish Tiwari, A. Mahapatra, Jagadish Jena, Minu Mohan

{"title":"Efficacy of broad-spectrum herbicide mixtures on weed flora in wet direct seeded rice (Oryza sativa L.) in the east coast plain region of India","authors":"S. Meher, Sanjoy Saha, Nitish Tiwari, A. Mahapatra, Jagadish Jena, Minu Mohan","doi":"10.31830/2348-7542.2024.roc-1076","DOIUrl":"https://doi.org/10.31830/2348-7542.2024.roc-1076","url":null,"abstract":"Wet direct seeded rice (DSR) attributing floristically diverse weed flora which emerges as main constraints for its successful cultivation. Broad spectrum herbicide mixtures can be acted as weapon for mitigating this issue by nullifying the challenges like weed shift, herbicide resistance etc. Consequently, an experiment was conducted at the Institute Research Farm of ICAR-National Rice Research Institute, Cuttack, situated in the east coast plain region of India specifically in mid-central table land agro-climatic zone of Odisha during the Kharif seasons of 2020 and 2021 to assess the effectiveness of different herbicide mixtures in managing weeds successfully. The experiment was laid out in Randomized Complete Block Design encompassing 12 treatments, viz., nine post-emergence herbicide mixtures (experimental combinations for testing), one post-emergence herbicide check, one weed-free (T11) (weed-free plots were maintained by hand weeding) and one weedy check (T12), replicated thrice. The net plot size was 5.8 × 3.8 m (22.04 m2). The test variety CR Dhan 203 was sown in 20 cm rows apart. Weed-free treatment (T11) was at par with fenoxaprop-p ethyl 6.7% EC (w/w) + ethoxysulfuron 15% WDG (w/w) (50+15) g/ha (T4) , penoxsulam 21.7% SC+ cyhalofop-butyl 12% EC (w/v) (25+100) g/ha (T3) , azimsulfuron 50% DF+ bispyribac sodium 10% SC (22+25) g/ha (T1), flucetosulfuron 10% WDG (w/w) + bispyribac sodium 10% SC (25+25) g/ha (T2) , bispyribac sodium 10% SC + ethoxysulfuron 15% WDG (w/w) (25+15) g/ha (T5) for high performance in terms of grain yield. Also showed lowest weed flora dynamics and relative weed density as well. Among the herbicide treatments, fenoxaprop-p ethyl 6.7% EC (w/w) + ethoxysulfuron 15% WDG (w/w) (50+15) g/ha (T4) registered the highest weed control efficiency (86.49%) and lowest weed index (6.69%), hence demonstrating as most viable and adoptable combinations for agricultural crop production.\u0000","PeriodicalId":21022,"journal":{"name":"Research on Crops","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141111550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Response of yield and its components of two wheat varieties to foliar spraying with stearic acid 两个小麦品种的产量及其成分对叶面喷洒硬脂酸的反应

Research on Crops Pub Date : 2024-05-22 DOI: 10.31830/2348-7542.2024.roc-1061

H. Baqir, N. H. Zeboon

{"title":"Response of yield and its components of two wheat varieties to foliar spraying with stearic acid","authors":"H. Baqir, N. H. Zeboon","doi":"10.31830/2348-7542.2024.roc-1061","DOIUrl":"https://doi.org/10.31830/2348-7542.2024.roc-1061","url":null,"abstract":"As a result of the excessive use of mineral fertilizers, it has led to an increase in the accumulation of pollutants in the soil and an imbalance in the elements present in it , leading to a decrease in crop productivity and quality, in addition to serious health problems for humans and animals, Therefore, attention in recent research has focused on the use of safe and environmentally friendly materials, including stearic acid, Therefore, a field experiment was conducted at Experiments Station affiliated to the Department of field crops, College of Agricultural Engineering Sciences, University of Baghdad during the winter season of 2022-2023, aiming to study the effect of foliar spraying with stearic acid on some traits of yield and its components of wheat. Randomized complete block design within split-plot arrangement with three replications was used. The study included two factors, the first (the main plots) represented two varieties of wheat (Buhooth 22 and Adina), while the second factor in the sub-plot represented spraying with stearic acid at three concentrations of 100, 200, and 300 mg/L, in addition to the control treatment (without spraying). The experiment results showed the superiority of the variety Buhooth 22 to Adina in most yield traits, as the highest average grain yield was recorded at 5.38 t/ha. Regarding the concentrations of the sprayed stearic acid, the results showed the superiority of the concentration 300 mg/L in number of spikes, weight of 1000 grain, grain yield, and harvest index. The concentration 300 mg/L didn’t differ significantly with the concentration mg/L in most traits, especially the total grain yield, which amounted to 5.88 t/ha. We conclude that Buhooth 22 cultivar and stearic acid with 200 mg/L concentration recorded the best results.\u0000","PeriodicalId":21022,"journal":{"name":"Research on Crops","volume":"59 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141112591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characters of Pandoraea, a bacterium associated with tomato (Solanum lycopersicum) using 16S rDNA from Limpopo Province, South Africa 南非林波波省番茄（Solanum lycopersicum）伴生细菌 Pandoraea 的 16S rDNA 分子特征

Research on Crops Pub Date : 2024-03-18 DOI: 10.31830/2348-7542.2024.roc-972sc

Gavin Geldenhuys

{"title":"Molecular characters of Pandoraea, a bacterium associated with tomato (Solanum lycopersicum) using 16S rDNA from Limpopo Province, South Africa","authors":"Gavin Geldenhuys","doi":"10.31830/2348-7542.2024.roc-972sc","DOIUrl":"https://doi.org/10.31830/2348-7542.2024.roc-972sc","url":null,"abstract":"Tomato is one of the premier vegetables in the world and is widely cultivated for higher yield and economic importance; thus, the area under cultivation is increasing. Pandoraea species can be a risky pathogenic bacterium to tomato plants, impacting the yield. They are gram-negative, motile, and rod-shaped. In 2022, the University of Limpopo conducted a study to identify and name the Pandoraea bacterium found in a tomato field located in Limpopo Province, South Africa. Molecular methods were employed, and the Chelex approach was utilized to extract the 16S rDNA marker, enabling the identification of bacteria present in the soil of tomato fields in South Africa. After conducting tests using specific primers to amplify the 16S rDNA, we have identified the bacterium as Pandoraea. Our analysis indicates that the strain of Pandoraea found in South Africa shares a 90% similarity with a population from Belgium (LR536859). Furthermore, maximum likelihood phylogenetic analysis indicates that this species is grouped with other Pandoraea species, with highly supported (100) bootstrap values. While the 16S rDNA method successfully identified this species, we recommend utilizing other DNA markers for a more comprehensive understanding of the Pandoraea phylogeny.\u0000","PeriodicalId":21022,"journal":{"name":"Research on Crops","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140232733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular study of Serratia marcescens, a bacterium associated with roots of maize (Zea mays L.), using 16S rDNA in Limpopo Province, South Africa 利用 16S rDNA 对南非林波波省玉米（Zea mays L.）根部相关细菌 Serratia marcescens 进行分子研究

Research on Crops Pub Date : 2024-03-18 DOI: 10.31830/2348-7542.2024.roc-980sc

Gavin Geldenhuys

{"title":"Molecular study of Serratia marcescens, a bacterium associated with roots of maize (Zea mays L.), using 16S rDNA in Limpopo Province, South Africa","authors":"Gavin Geldenhuys","doi":"10.31830/2348-7542.2024.roc-980sc","DOIUrl":"https://doi.org/10.31830/2348-7542.2024.roc-980sc","url":null,"abstract":"Maize the most important grain crop in South Africa produced under diverse environments is prone to dangerous pathogenic bacteria which needs better control and management. One of the bacteria that can affect maize yield is Serratia a gram-negative, facultatively anaerobic, motile bacterium. Colonies on (aerobic) nutrient agar plates are circular and have a dark red to pink colour. This study isolated and identified Serratia bacterium from maize fields in Limpopo, South Africa. The molecular study was done in 2022 at the Aquaculture Research Unit, University of Limpopo. To identify the bacterium associated with maize, for extraction DNA Chelex method was used then 16S rDNA marker was used. The bacterium was identified as S. marcescens. The Nblast analysis showed South African S. marcescens had 97% similarity with a population from Brazil (MH127785), and South Korea (MN082049). Phylogenetic analysis using maximum likelihood placed this species with those molecularly identified as S. marcescens in the same clade with highly supported (100) bootstrap values. In conclusion, this species is identified using 16S rDNA properly. However, using other DNA markers to understand S. marcescens phylogeny better is recommended.\u0000","PeriodicalId":21022,"journal":{"name":"Research on Crops","volume":"41 16","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140231525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular study of Enterobacter associated with roots of maize (Zea mays L.) using 16S rDNA in Limpopo Province, South Africa 利用 16S rDNA 对南非林波波省玉米（Zea mays L.）根部相关肠杆菌进行分子研究

Research on Crops Pub Date : 2024-03-18 DOI: 10.31830/2348-7542.2024.roc-981sc

Gavin Geldenhuys

{"title":"Molecular study of Enterobacter associated with roots of maize (Zea mays L.) using 16S rDNA in Limpopo Province, South Africa ","authors":"Gavin Geldenhuys","doi":"10.31830/2348-7542.2024.roc-981sc","DOIUrl":"https://doi.org/10.31830/2348-7542.2024.roc-981sc","url":null,"abstract":"One of the most significant grain crops in South Africa, white maize, is grown all throughout the country in a wide variety of climates and soil types due to its high economic value. White maize cultivation is expanding at a staggering rate, for which accurate identification of root-associated bacteria is necessary. Among the various bacterium affecting maize, Enterobacter species a gram-negative, motile, non-spore-forming, rod-shaped, facultatively anaerobic bacteria are dangerous pathogenic bacteria causing drastic reduction in yield if not properly identified and managed. Therefore, this study was conducted to isolate and identify Enterobacter from maize fields in Limpopo Province, South Africa. The molecular study was done in 2022 at the Aquaculture Research Unit, University of Limpopo. To identify the bacterium associated with maize, for extraction DNA, the Chelex method was used then 16S rDNA marker was used. The bacterium was identified as Enterobacter. The Nblast analysis showed South African Enterobacter has 97% similarity with a population from Korea (KC355340). Phylogenetic analysis using maximum likelihood placed this species with those molecularly identified as Enterobacter in the same clade with highly supported (100) bootstrap values. In conclusion, this species is identified using 16S rDNA properly. However, other DNA markers are recommended to better understand Enterobacter phylogeny.\u0000","PeriodicalId":21022,"journal":{"name":"Research on Crops","volume":"305 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140233214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characters of Pseudomonas cichorii using 16S rDNA isolated from spinach, Limpopo Province, South Africa 利用 16S rDNA 鉴定从南非林波波省菠菜中分离出的 cichorii 假单胞菌的分子特征

Research on Crops Pub Date : 2024-03-18 DOI: 10.31830/2348-7542.2024.roc-956

Mehrnoush Aminisarteshnizi

{"title":"Molecular characters of Pseudomonas cichorii using 16S rDNA isolated from spinach, Limpopo Province, South Africa ","authors":"Mehrnoush Aminisarteshnizi","doi":"10.31830/2348-7542.2024.roc-956","DOIUrl":"https://doi.org/10.31830/2348-7542.2024.roc-956","url":null,"abstract":"Pseudomonas is a genus of Gram-negative, Gammaproteobacteria, belonging to the family Pseudomonadaceae. Pseudomonas cichorii is a Gram-negative soil bacterium that is pathogenic to plants. It has a wide host range and can have an important economic impact on lettuce, celery, and chrysanthemum crops. Pseudomonas cichorii can be a risky pathogenic bactererium in vegetables, including spinach, and therefore have an effect on the fresh production of spinach. This study was conducted to isolate and identify P. cichorii from a spinach field in Limpopo Province, South Africa. The molecular study was conducted in 2023 at Limpopo University to identify the bacterium from South Africa's soils using 16S rDNA marker. The DNA was extracted using the Chelex method. The bacterium was identified as P. cichorii. The Nblast analysis showed South African P. cichorii had 99% similarity with a population from South Africa (FJ477103). Phylogenetic analysis using maximum likelihood placed this species with those molecularly identified as P. cichorii in the same clade with highly supported (100) bootstrap values. The species was identified using 16S rDNA however, the use of other DNA markers can be evaluated for a better understanding of P. cichorii phylogeny.\u0000","PeriodicalId":21022,"journal":{"name":"Research on Crops","volume":"289 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140233325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0