Rejuvenation research最新文献

{"title":"Neuroprotective Potential of Hydroalcoholic Extract of <i>Centella asiatica</i> Against 3-Nitropropionic Acid-Induced Huntington's Like Symptoms in Adult Zebrafish.","authors":"Vishal Kumar,&nbsp;Charan Singh,&nbsp;Arti Singh","doi":"10.1089/rej.2022.0036","DOIUrl":"https://doi.org/10.1089/rej.2022.0036","url":null,"abstract":"<p><p>Huntington's disease (HD) is an inherited neurodegenerative disease. 3-Nitropropionic acid (3-NP) causes increased reactive oxygen species production and neuroinflammation. <i>Centella asiatica</i> (CA) is a strong antioxidant. The aim of this study is to investigate the effect of hydroalcoholic extract of <i>C. asiatica</i> (HA-CA) on 3-NP-induced HD in adult zebrafish. Adult zebrafish (∼5-6 months old) weighing 470 to 530 mg was used and treated with 3-NP (5 mg/kg intraperitoneal [<i>i.p.</i>]). The animals received HA-CA (80 and 100 mg/L) daily for up to 28 days in water. Tetrabenazine (3 mg/kg <i>i.p.</i>) was used as a standard drug. We have done an open field test (for locomotor activity), a novel tank diving test (for anxiety), and a light and dark tank test (for memory), followed by biochemical analysis (acetyl-cholinesterase [AchEs], nitrite, lipid peroxidation [LPO], and glutathione [GSH]) and histopathology to further confirm memory dysfunctions. 3-NP-treated zebrafish exhibit reductions in body weight, progressive neuronal damage, cognition, and locomotor activity. The HA-CA group significantly reduced the 3-NP-induced increase in LPO, AchEs, and nitrite levels while decreasing GSH levels. Oral administration of HA-CA (80 or 100 mg/L) significantly reduces 3-NP-induced changes in body weight and behaviors, in addition to neuroinflammation in the brain by lowering tumor necrosis factor-α and interleukin-1β levels. Moreover, HA-CA significantly decreases the 3-NP-induced neuronal damage in the brain. HA-CA ameliorates neurotoxicity and neurobehavioral deficits in 3-NP-induced HD-like symptoms in adult zebrafish.</p>","PeriodicalId":20979,"journal":{"name":"Rejuvenation research","volume":"25 6","pages":"260-274"},"PeriodicalIF":2.6,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10342037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"October 2022 Editorial.","authors":"Irina Conboy","doi":"10.1089/rej.2022.0049","DOIUrl":"https://doi.org/10.1089/rej.2022.0049","url":null,"abstract":"na.","PeriodicalId":20979,"journal":{"name":"Rejuvenation research","volume":"25 5","pages":"207"},"PeriodicalIF":2.6,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33490635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Berberine Ameliorates Lipopolysaccharide-Induced Cognitive Impairment Through SIRT1/NRF2/NF-κB Signaling Pathway in C57BL/6J Mice.","authors":"Nan Chen,&nbsp;Xin-Chen Wang,&nbsp;Ling-Ling Fan,&nbsp;Yu-Huang Zhu,&nbsp;Qi Wang,&nbsp;Yun-Bo Chen","doi":"10.1089/rej.2022.0023","DOIUrl":"https://doi.org/10.1089/rej.2022.0023","url":null,"abstract":"<p><p>The inflammatory response is the stress reactions to infection or injury so as to help the body return to normal as soon as possible. In central nervous system, the overactivated immune system causes irreversible damage to neurons and synapses, which results in cognitive impairment. Berberine, an isoquinoline alkaloid extracted from <i>Coptidis Rhizoma</i>, plays a powerful role in anti-inflammation. It has been reported that berberine significantly improved the decline of cognitive ability. Therefore, we carried out this work to find out the specific mechanism. We tested behaviorally that berberine administration did improve lipopolysaccharide (LPS)-induced cognitive impairment in C57BL/6J mice. We found that berberine reduced neuronal damage in the hippocampus by Nissl staining, and verified by western blot and immunofluorescence that berberine improved LPS-induced cognitive impairment through the SIRT1/nuclear factor E2-related factor 2 (NRF2)/nuclear factor-kappaB (NF-κB) signaling pathway. The results showed that berberine plays an anti-inflammatory and antioxidant role by targeting SIRT1/NRF2/NF-κB signaling pathway so as to reduce the cognitive impairment and neuronal damage caused by LPS in C57BL/6J mice. Berberine preprotection increased the expression of heme oxygenase-1 (HO-1) after activating NRF2 and inhibited the activation of NF-κB and the release of inducible NO synthase, which may be related to berberine activating SIRT1. However, the effect of reducing inflammatory response was inhibited after using SIRT1 inhibitor EX527 <i>in vitro</i>. This research explains the significance of anti-inflammatory in the treatment of cognitive impairment from different angles.</p>","PeriodicalId":20979,"journal":{"name":"Rejuvenation research","volume":" ","pages":"233-242"},"PeriodicalIF":2.6,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40421072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Downregulation of <i>SOX21-AS1</i> Alleviated Cisplatin Resistance in Cervical Cancer Through Epithelial-Mesenchymal Transition Inhibition.","authors":"Jing Tian,&nbsp;Ze Li,&nbsp;Yuanyuan Jiang,&nbsp;Wenjin Gu,&nbsp;Enqi Kong,&nbsp;Quan Hao,&nbsp;Beihua Kong,&nbsp;Li Sun","doi":"10.1089/rej.2022.0009","DOIUrl":"https://doi.org/10.1089/rej.2022.0009","url":null,"abstract":"<p><p>Cisplatin is widely used in chemotherapies in cervical cancer (CC). Nevertheless, drug resistance in cancer patients poses a major threat to efficacy of treatment. To explore the underlying modulatory mechanism of <i>SOX21-AS1</i> in cisplatin resistance in CC cell and mice models, Gepia database was referred for <i>SOX21-AS1</i> expression in cancer tissues and normal ones. Reverse transcription quantitative real-time polymerase chain reaction was used to measure the differential expression of <i>SOX21-AS1</i> in parental Siha cells and cisplatin-resistant Siha/DDP cells. Luciferase reporter gene assays were conducted to verify putative bindings between <i>SOX21-AS1</i> and <i>miR-9-3p</i>. Western blot method was employed to evaluate the changes in cleaved-caspase 7 protein expression. Cisplatin resistance was evaluated in each transfected group using cell counting kit 8 method after cells were exposed to cisplatin (0, 7.5, 15, 30, 60, 120, and 240 μg/mL) for 24 hours. Flow cytometry method was used to measure the apoptosis rates. Cell migration and invasion were measured using Transwell assays. Immunofluorescence method was applied to observe epithelial to mesenchymal transition (EMT) markers, including E-cadherin, Snail, matrix metalloproteinase (MMP)3, and MMP9. Siha/DDP cell groups stably transfected with sh-NC and sh-<i>SOX21-AS1</i> were injected through tail vein of Balb/C mice. Lung tissue sections were used for hematoxylin and eosin staining and immunohistochemistry analysis. <i>SOX1-AS1</i> expression was higher in cancer tissues than normal ones and was also higher in Siha/DDP rather than Siha cells. <i>SOX21-AS1</i> was targeted by <i>miR-9-3p</i> in CC cells. Downregulation of <i>SOX21-AS1</i> or overexpression of <i>miR-9-3p</i> inhibited cisplatin resistance in Siha/DDP cells and reduced cell invasion and migration and attenuated EMT progression. <i>In vivo</i>, the <i>SOX21-AS1</i> knockdown led to less severe lung metastasis. Downregulation of <i>SOX21-AS1</i> alleviated cisplatin resistance in CC through EMT inhibition.</p>","PeriodicalId":20979,"journal":{"name":"Rejuvenation research","volume":"25 5","pages":"243-252"},"PeriodicalIF":2.6,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33446910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of Thymus Rejuvenation in Elderly Macaques.","authors":"Yan-Ying Wang,&nbsp;Le Chang,&nbsp;Gao-Hong Zhu,&nbsp;Gong-Hua Li,&nbsp;Qing-Peng Kong,&nbsp;Long-Bao Lv,&nbsp;Qiang Wang,&nbsp;Chuan Tian,&nbsp;Ye Li,&nbsp;Xiang-Qing Zhu,&nbsp;Xing-Hua Pan","doi":"10.1089/rej.2022.0013","DOIUrl":"https://doi.org/10.1089/rej.2022.0013","url":null,"abstract":"<p><p>Senile thymus atrophy is an important factor leading to decreased immune function. Repairing the atrophic thymus tissue structure, rebuilding immune function, and replenishing the number of exogenous stem cells may be ideal methods. In this study, bone marrow mesenchymal stem cells were intravenously infused into elderly macaques. We found that thymus volume was substantially increased, some thymus tissue regeneration was observed, the degree of thymus tissue fibrosis decreased, collagen fiber deposition decreased, cortical and medulla structures emerged gradually, the number of apoptotic cells decreased significantly, and the expression of apoptosis-related proteins decreased. For the effects of stem cell therapy on aging-related genes, we performed transcriptomic analysis of thymus tissue. The results show the expression pattern of the tissue transcriptome tended to be similar to the thymus expression pattern in young macaques compared with the elderly group, reverse aging-related proteins. Based on the results, it is suggested that stem cell therapy is an ideal method to prevent or reverse the aging of the thymus.</p>","PeriodicalId":20979,"journal":{"name":"Rejuvenation research","volume":" ","pages":"223-232"},"PeriodicalIF":2.6,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40646347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cellular and Mitochondrial Quality Control Mechanisms in Maintaining Homeostasis in Aging.","authors":"Urvashi Langeh,&nbsp;Vishal Kumar,&nbsp;Anoop Kumar,&nbsp;Pradeep Kumar,&nbsp;Charan Singh,&nbsp;Arti Singh","doi":"10.1089/rej.2022.0027","DOIUrl":"https://doi.org/10.1089/rej.2022.0027","url":null,"abstract":"<p><p>Aging is a natural process in all living organisms defined as destruction of cell function as a result of long-term accumulation of damages. Autophagy is a cellular house safeguard pathway that is responsible for degrading damaged cellular organelles. Moreover, it maintains cellular homeostasis, controls lifetime and longevity. Damaged mitochondrial accumulation is a characteristic of aging that is associated with neurodegeneration. Mitochondria function as a principal energy source through supplying adenosine-5'-triphosphate (ATP) through oxidative phosphorylation that serves as fuel for neuronal function. Mitophagy and mitochondrial-specific autophagy play an important role in maintenance of neuronal health through the removal of dysfunctional and aged mitochondria. The mitochondrial quality control system involves different strategies for protecting against mitochondrial dysfunction and maintaining healthy mitochondria in cells. Mitochondrial function protection could be a strategy for the promotion of neuroprotection. Mitophagy could be an effective target for drug discovery. Therefore, further detailed studies for mechanism of mitophagy will advance our mitochondrial phenotype knowledge and understanding to disease pathogenesis. This review mainly focuses on aging-mediated mechanism of autophagy and mitophagy for maintaining the cellular homeostasis and longevity.</p>","PeriodicalId":20979,"journal":{"name":"Rejuvenation research","volume":" ","pages":"208-222"},"PeriodicalIF":2.6,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40517116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"August 2022 Editorial.","authors":"Irina Conboy","doi":"10.1089/rej.2022.0042","DOIUrl":"https://doi.org/10.1089/rej.2022.0042","url":null,"abstract":"Trapped Charged Particles and Fundamental Interactions: I. Invited papers from the conference, Wildbad Kreuth, Germany, 25-30 August 2002 This international conference was the third in the series on Trapped Charged Particles and Fundamental Interactions (TCPFI). This meeting, following on from those held in Lysekil (Sweden) in 1994 and Monterey, CA (USA) in 1998, was organized in Wildbad Kreuth (Germany) within the framework of the WE-Heraeus seminar series. The conference highlighted the wide application and multi-disciplinary nature of the field of trapped charged particles with talks and posters covering topics from elementary particle and nuclear physics, one-component plasma and beam physics, through to precision metrology and quantum information processing. To illustrate the wide application and highlight the new results and technical challenges of the trapped charged particle physics presented at this conference, Journal of Physics B: Atomic, Molecular and Optical Physics (JPB) is publishing two special sections containing articles based on talks and presentations from the TCPFI conference. These articles were carefully selected by the conference organizers and reviewed by JPB. The first special section (starting on p 499 of this issue) contains papers based on the invited talks. The second section, containing articles based on selected oral and poster presentations, will appear in issue 5 (publication date 14 March 2003) of JPB. Finally, the organizers would like to thank the international advisory committee (see below) for their support, and the delegates for making it a very successful and interesting conference. The beautiful conference site at Wildbad Kreuth (shown in the figure below) and the genuine Bavarian entertainment generated the atmosphere for a successful exchange of new ideas between the renowned experts in the fields and a large number of younger scientists. Martin Groß, Dietrich Habs, Wolfgang Lange, Ulrich Schramm and Herbert Walther Guest Editors and Organizers International Advisory Committee I Bergström R van Dyck Jr G Gabrielse D Habs H J Kluge S Pape Moller D Schneider R Schuch N Severijns H Walther D Wineland","PeriodicalId":20979,"journal":{"name":"Rejuvenation research","volume":" ","pages":"159"},"PeriodicalIF":2.6,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40618763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aqueous Extract from <i>Astragalus membranaceus</i> Can Improve the Function Degradation and Delay Aging on <i>Drosophila melanogaster</i> Through Antioxidant Mechanism.","authors":"Jianqin Zhang,&nbsp;Yuqi Qiao,&nbsp;Daqi Li,&nbsp;Shenghui Hao,&nbsp;Fusheng Zhang,&nbsp;Xubo Zhang,&nbsp;Aiping Li,&nbsp;Xuemei Qin","doi":"10.1089/rej.2021.0081","DOIUrl":"https://doi.org/10.1089/rej.2021.0081","url":null,"abstract":"<p><p>Astragali Radix is the dry root of the leguminous plants <i>Astragalus membranaceus</i> (Fisch.) Bge. Var. <i>mongholicus</i> (Bge.) Hsiao and <i>A. membranaceus</i> (Fisch.) Bge. Astragali Radix is mostly used clinically as a decoction. A number of pharmacological studies show that <i>Astragalus</i> extract can increase telomerase activity and has antioxidation, anti-inflammatory, immune regulation, anticancer, lowering blood lipid, lowering blood sugar, and other effects. However, the anti-aging mechanism of aqueous extract from Astragali Radix (ARE) is still unclear. In this study, we evaluated the anti-aging effect of ARE on <i>Drosophila melanogaster</i> and investigated the underlying mechanism. The results of life span assay showed that 1.25 mg/mL of ARE can significantly prolong the life span of <i>D. melanogaster</i> in a natural aging model and protect against H₂O₂ and paraquat. Meanwhile, ARE can improve the climbing ability and food intake of flies. Metabolomics and the glutamate content assay suggested that ARE prevented an age-dependent increase in glutamate levels in <i>D. melanogaster</i>. Furthermore, ARE showed a dose-dependent effect on the scavenging ability of α, α-diphenyl-β-picrylhydrazyl <i>in vitro</i>. Superoxide dismutase and catalase activities in the aging group also increased after the intervention of ARE. The data and the findings described here support the notion that ARE may play a preventive role in aging by improving the climbing ability, eliminating harmful free radicals accumulated in <i>D. melanogaster</i> and triggering antioxidant responses.</p>","PeriodicalId":20979,"journal":{"name":"Rejuvenation research","volume":" ","pages":"181-190"},"PeriodicalIF":2.6,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40120767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three Month Heterochronic Parabiosis Has a Deleterious Effect on the Lifespan of Young Animals, Without a Positive Effect for Old Animals.","authors":"Tatiana Yankova,&nbsp;Tatiana Dubiley,&nbsp;Dmytro Shytikov,&nbsp;Iryna Pishel","doi":"10.1089/rej.2022.0029","DOIUrl":"https://doi.org/10.1089/rej.2022.0029","url":null,"abstract":"<p><p>Our previous study showed that an exchange of blood between heterochronic parabionts for 3 months did not rejuvenate the immune system of the old partners. Moreover, the young immune system became more aged and began to function according to the \"old\" principle. Does this forced aging affect all systems of the organism in this model? We checked the levels of corticosterone, testosterone, insulin-like growth factor 1 (IGF-1), insulin, and thyroxine in the blood of heterochronic parabionts, but did not find significant changes compared with age-related controls. Since numerous data support the possibility of rejuvenation of the brain, muscles, and other tissues using the model of heterochronic parabiosis, as well as opposite data, we planned to assess the overall effect of this long-term blood exchange on the rate of organism aging. We measured the life span of animals whose blood was exchanged for 3 months and then were disconnected. Median and maximum life expectancy decreased in young heterochronic parabionts compared with the isochronic control. Old heterochronic parabionts showed only a small trend toward an increase in the median life span, but it was not statistically significant, and the maximum life span did not change compared with the isochronic parabionts. These data support our assumption that old blood contains factors capable of inducing aging in young animals. The mechanism of selective suppression of aging factor production in the organism could be a key research field for life extension.</p>","PeriodicalId":20979,"journal":{"name":"Rejuvenation research","volume":" ","pages":"191-199"},"PeriodicalIF":2.6,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40393561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Link between senescence and cell fate: Senescence-associated secretory phenotype (SASP) and its effects on stem cell fate transition.","authors":"Y. Pan, Zhenzhen Gu, Yansi Lyu, Yi Yang, Man-Hon Chung, Xiaohua Pan, S. Cai","doi":"10.1089/rej.2022.0021","DOIUrl":"https://doi.org/10.1089/rej.2022.0021","url":null,"abstract":"Senescence is a form of durable cell cycle arrest elicited in response to a wide range of stimuli. Senescent cells remain metabolically active and secrete a variety of factors collectively termed senescence-associated secretory phenotype (SASP). SASP is highly pleiotropic and can impact numerous biological processes in which it has both beneficial and deleterious roles. The underlying mechanisms by which SASP exerts its pleiotropic influence remain largely unknown. SASP serves as an environmental factor, which regulates stem cell differentiation and alters its routine. The latter can potentially be accomplished through dedifferentiation, transdifferentiation, or reprogramming. Behavioral changes that cells undergo when exposed to SASP are involved in several senescence-associated physiological and pathological phenomena. These findings provide clues for identifying possible interventions to reduce the deleterious effects without interfering in the beneficial outcomes. Here, we discuss the multifaced effects of SASP and the changes occurring in cellular states upon exposure to SASP factors.","PeriodicalId":20979,"journal":{"name":"Rejuvenation research","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2022-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42060215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}