RESEARCH RESULTS IN BIOMEDICINE最新文献

{"title":"Study of the cytokine genes SNPs association with the characteristics of the immunological status of children with recurrent respiratory infection","authors":"I. A. Makarova, E. Teplyakova, M. A. Kazumian, O. Lyangasova, A. Aleksandrova, E. V. Mashkina, T. P. Shkurat","doi":"10.18413/2658-6533-2022-8-3-0-3","DOIUrl":"https://doi.org/10.18413/2658-6533-2022-8-3-0-3","url":null,"abstract":"Background: Herpesvirus infections are one of the most common human infections that play a special role in children with recurrent respiratory infection cohort formation. The immunological status of such children is characterized by an imbalance in all links of immunity. The aim of the study: To study the immunological status of RRI children and to analyze the association of SNP -330T>G in IL2 and -1082G>A in IL10 with the relative and absolute values of the main subpopulations of lymphocytes. Materials and methods: In our study, we enrolled 76 children between 10 and 17 years old who had acute respiratory infections at least 5 times a year. Flow cytometry was used for lymphocyte subpopulations analysis. Polymorphic variants were determined by allele-specific PCR. Results: In anti-NA EBV IgG and anti-CMV IgG positive children with AA genotype for the -1082G>A IL10 polymorphism the average relative number of B-lymphocytes was 9.6%, which is below normal values (р=0.01). The average number of B-lymphocytes for heterozygotes was 12.4% (р=0.01). In the subgroup children with GG genotype, the average number of B-lymphocytes was 16.7% (р=0.01). In children with AA genotype the average level of NK cells was 19.3% (р=0.04). Children with GG genotype had lower level of NK cells (10.9%) (р=0.04). Conclusion: Our studies revealed a relationship between the immune status indicators and the genotypes for the -1082G>A IL10 polymorphism in anti-NA EBV IgG and anti-CMV IgG positive RRI children group. The -1082AA genotype of the IL10 is associated with a decrease in the relative and absolute number of B-lymphocytes, as well as with an increase in the relative number of NK cells. RRI children with -1082GG genotype tend to increase the relative and absolute number of B-lymphocytes, and to decrease the relative number of NK-cells.","PeriodicalId":20921,"journal":{"name":"RESEARCH RESULTS IN BIOMEDICINE","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83286264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of polymorphism rs10841855 in the glycogen synthase 2 gene with hypercholesterolemia and type 2 diabetes mellitus","authors":"I. Azarova, Yaroslava E. Korvyakova, Alexander O. Glotov, V. E. Ivakin, A. Polonikov","doi":"10.18413/2658-6533-2022-8-2-0-1","DOIUrl":"https://doi.org/10.18413/2658-6533-2022-8-2-0-1","url":null,"abstract":"Background: Glycogen synthase 2 (GYS2) catalyzes a key step in glycogenesis in the liver. Loss-of-function mutations in the GYS2 gene are associated with type 0a glycogenosis with characteristic fasting hypoglycemia and postprandial hyperglycemia. The association of the single nucleotide polymorphism rs10841855 (G>T) with the risk of type 2 diabetes mellitus (T2D) was first established in the European population, but data on the effect of this variant on the predisposition to T2D in the inhabitants of the Slavic ethnic group of Central Russia are not available in the literature. The aim of the study: To investigate the association of polymorphism rs10841855 (G>T) GYS2 with the risk of T2D in residents of Central Russia. Materials and methods: The study included 2668 unrelated individuals of Slavic origin, including 1387 patients with T2D and 1281 healthy volunteers. Genotyping of the rs10841855 polymorphism of the GYS2 gene was performed by the MassArray Analyzer-4 genetic analyzer. SNPStats software was used for statistical analysis of the data. Results: Linear regression established an association of the alternative rs10841855-T allele (OR 1.25; 95% CI 1.09-1.43; P=0.001) and the rs10841855-G/T genotype (OR 1.37; 95% CI 1, 13-1.66; P=0.0064) of GYS2 with an increased risk of T2D. The sex- and BMI-stratified analysis showed that the association of the rs10841855-G/T genotype (OR 1.71; 95% CI 1.32-2.22; P=0.0001) occurred only in females with BMI>25 kg/m2. In addition, carriage of the rs10841855-T allele was associated with a higher level of total cholesterol in the blood plasma of T2D patients (P=0.0068). According to the Roadmap Epigenomic Consortium, the rs10841855-T allele is associated with H3K4 histone methylation in the enhancer and promoter regions of the GYS2 gene in the liver. Conclusion: The association of rs10841855 of the GYS2 gene with an increased risk of T2D in residents of Central Russia was validated for the first time. The association may be explained by the low transcriptional activity of the glycogen synthase 2 gene in the carriers of the alternative allele of this polymorphism.","PeriodicalId":20921,"journal":{"name":"RESEARCH RESULTS IN BIOMEDICINE","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81123568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of stem cell markers in meningioma among Sudanese patients","authors":"Aldeaf Sawsan A.H., Alsadig Gassoum, M. A. Arbab, Ibrahim Montaser A., Nihad Elsadig, S. Elhassan, Nahla E. Abdelrahem, Imad M. Fadl Elmula, A. Elhassan","doi":"10.18413/2658-6533-2022-8-1-0-1","DOIUrl":"https://doi.org/10.18413/2658-6533-2022-8-1-0-1","url":null,"abstract":"Background: Meningioma is the second most common primary intracranial tumor of the central nervous system, surgical total excision of meningioma offers a better survival to patients; however, a significant proportion of histological variant recur within 5 years despite complete excision. Chemotherapy is generally unsuccessful in treating meningiomas, so refractory and recurrence meningioma are treated with palliative surgery and radiotherapy. In this context, certain therapeutic approaches based on effective molecular biology are needed. In ecent years, the so-called cancer stem cells (CSC) have emerged; these cells can be identified by stem cell markers among many other cancers, but there is no unique marker found in all stem cells, as their phenotype varies considerably among different cells and species. The aim of the study: To characterize the RNA (cDNA) of CD 44, CD 73 and CD 105 genes as stem cell markers in meningioma among Sudanese patients and to correlate the PCR findings of CD 44, CD 73 and CD 105 genes with meningioma histological variants. Materials and methods: Using Innu PREP RNA – MiniKit (Analytic Jena) 56 tissue samples radiologically diagnosed as meningioma were immediately processed for RNA extraction and cDNA synthesis. The PCR was done using Maxime PCR premix Kit (i-Taq) entrobiotechnology. Results: This study included 56 tumor samples; 54 samples were confirmed histologically to be meningioma; 40 (74.07%) were female and 14 (25.93%) ere male. The majority of the patients were Afro-Asian (68.4), followed by Niger-Congo (22.8), and all patients had headache. CD44 was expressed in all meningioma samples (100%), CD73 was positive in 61% and negative in 39%, CD105 was positive in 89% of the samples. Conclusion: Our results showed that, the expression of cancer stem cell markers in meningioma was inconsistent within the same class of meningioma; moreover, the expression of CD44, CD73 and CD105 markers could confirm the presence of cancer stem cells in our meningioma samples.","PeriodicalId":20921,"journal":{"name":"RESEARCH RESULTS IN BIOMEDICINE","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79116727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wound healing effect of organometallic zinc complex in the rat model of planar skin wound","authors":"S. Lebedeva, P. A. Galenko-Yaroshevsky (Jr.), Stanislav Melnik, S. Kozin, T. Demura, Yaroslav R. Arshinov, Maria V. Svistakova, E. Grigorevskikh, P. Galenko-Yaroshevsky","doi":"10.18413/2658-6533-2022-8-1-0-5","DOIUrl":"https://doi.org/10.18413/2658-6533-2022-8-1-0-5","url":null,"abstract":"Background: The complex and dynamic process of wound healing involves the stages of inflammation, proliferation and maturation of the connective tissue of the scar. Zinc, as an essential trace element, plays an important physiological role at each stage of wound remodeling. Zinc increases the migration and proliferation of keratinocytes, participates in the reepithelization of the epidermis, has anti-inflammatory, antioxidant, immunomodulatory and antimicrobial action. The aim of the study: Comparative study of the effect of the zinc complex of the N-isopropenylimidazole derivative under the code name Pilim-1 on the course of wound uninfected process in modeling of planar skin wound in rats. Materials and methods: 1% gel of Pilim-1 sodium with carboxymethyl cellulose gel as a base has been studied. Methyluracil (10% ointment for topical application) was selected as reference preparation. A model of a \"planar skin wound\" was reproduced on 24 anesthetized outbred adult male white rats. The degree of healing was evaluated for 28 days by weight by checking the wound area. Wound skin bioptats were taken on day 29 after the start of treatment. On histological sections, the presence of capillaries, fibroblasts, epitheliocytes, white blood cells, the degree of severity of granulation tissue and its maturity, presence of necrosis and inflammation were evaluated. Results: Local application of 1% Pilim-1 gel was found to reduce the healing time of wounds compared to the control group of animals. In animals treated with Pilim-1, complete wound regeneration was noted on the 24 day. On days 10, 21, 24, 29, a statistically significant decrease in the size of the wound was Краткое сообщение Short communication Научные результаты биомедицинских исследований. 2022;8(1):71-81 Research Results in Biomedicine. 2022:8(1):71-81 73 revealed in this group compared to the control group. In the group of animals treated with methyluracil, complete wound healing was observed only in 3 rats on the 29th and 24th days of observation. On histological sections, signs of complete epithelization, restoration of skin epithelialization and the presence of mature granulation tissue were noted. Conclusion: The identified regenerative effect of the zinc complex is of interest for further study in order to develop an effective agent that stimulates reparative regeneration of wounds.","PeriodicalId":20921,"journal":{"name":"RESEARCH RESULTS IN BIOMEDICINE","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81575887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of N-terminal telopeptide-1 with BMD in patients with osteopenia and osteoporosis","authors":"Modagan P, Leena Chand","doi":"10.18413/2658-6533-2022-8-1-0-6","DOIUrl":"https://doi.org/10.18413/2658-6533-2022-8-1-0-6","url":null,"abstract":"Background: Osteoporosis is a common disease of aging population, causing fractures and raising mortality and morbidity. Standard diagnosis usually depends on measuring bone mineral density using a dual energy X-ray absorptiometry. But it does not provide information regarding bone formation or resorption. Bone turnover markers provide information regarding bone formation or resorption before the structural changes in bone occur. The aim of the study: This study was undertaken to investigate the effectiveness of N-terminal telopeptide-1 in the diagnosis of osteopenia and osteoporosis. Materials and methods: The case-control study was conducted in 170 individuals from the Department of Orthopedic Surgery. They were divided into three groups based on bone mineral density; group I with normal BMD (n=57), group II with osteopenia (n=62) and group III with osteoporosis (n=51). Institutional ethics committee approval was obtained. Written informed consent was obtained from all the study participants. Serum N-terminal telopeptide-1 (NTX-1) was analyzed by ELISA. Statistical analysis was performed using the SPSS 16.0 version for Windows. The continuous variables were expressed as mean ± SD. ANOVA with Tukey’s HSD and ROC curve analysis were performed. Odds ratio was analyzed. Pearson correlation coefficient was obtained between BMD and NTX1. P value <.05 was considered statistically significant. Results: There was a significant increase in serum N-terminal telopeptide-1 across the groups. NTX-1 values showed correlation with BMD. The area under the curve during analysis of NTX-1 in patients with normal BMD and osteopenia and osteoporosis were 0.697 and 0.592 respectively. Conclusion: The participants were in the obese as well as over-weight category. Serum N-terminal telopeptide-1 was significantly increased across the groups indicating that bone turnover markers are associated with progression of osteoporosis.","PeriodicalId":20921,"journal":{"name":"RESEARCH RESULTS IN BIOMEDICINE","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81865009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of sodium-glucose cotransporter type 2 inhibitors on the dynamics of functional and structural disorders in the simulation of post-contrast acute kidney injury","authors":"V. Y. Poltev, D. A. Kostina, N. I. Nesterova, I. Iezhitsa","doi":"10.18413/2658-6533-2022-8-1-0-4","DOIUrl":"https://doi.org/10.18413/2658-6533-2022-8-1-0-4","url":null,"abstract":"","PeriodicalId":20921,"journal":{"name":"RESEARCH RESULTS IN BIOMEDICINE","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89658042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical and morphological markers of susceptibility to prolonged and postterm pregnancies","authors":"","doi":"10.18413/2658-6533-2022-8-1-0-9","DOIUrl":"https://doi.org/10.18413/2658-6533-2022-8-1-0-9","url":null,"abstract":"","PeriodicalId":20921,"journal":{"name":"RESEARCH RESULTS IN BIOMEDICINE","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87534750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patients' attitude towards coercion and violence in providing stationary psychiatric aid: ethical and preventive aspects","authors":"N. Rzhevskaya, Kira S. Merzlikina, V. Ruzhenkova, Konstantin Yu. Retiunskii, Inna I. Zamyatina, O. Shiryaev","doi":"10.18413/2658-6533-2022-8-1-0-8","DOIUrl":"https://doi.org/10.18413/2658-6533-2022-8-1-0-8","url":null,"abstract":"Background: People with mental disorders are most vulnerable in respect to their rights and legitimate interests wile obtaining psychiatric aid. The aim of the study: To develop recommendations for improving the conditions of stay and treatment of persons with mental disorders in psychiatric clinics based on the study of the frequency and forms of coercion and violence applied to patients, and their personal assessment. Materials and methods: Using the medico-sociological method and the modified Bogardus social distance scale, 271 patients were examined in 3 regional psychiatric hospitals: 110 men and 161 women. Results: Patients of psychiatric clinics are socially maladapted, uncritical to their disease. They prefer not to have close relationships with people with mental disorders. Women are often subjected to coercion and violence in a psychiatric clinic. Patients justify coercion in treatment by psychiatrists. The use of physical restraint is considered the prerogative of junior medical staff, but the personal participation of a psychiatrist or the involvement of other patients in cases of threat to others is allowed. A quarter of patients speak out against the use of coercion in the hospital. Compulsory treatment of persons with mental disorders is justified by most patients if their well-being or public safety is in danger. The hospital considers it necessary to control drug treatment and force-feeding when patients refuse to eat. Most patients are not completely satisfied or indifferent to the conditions of living and treatment in the clinic. Conclusion: Creating a comfortable environment in psychiatric clinics requires the development of standards for equipping the wards and premises of the department. Minimization of the use of physical restraint and coercion requires clinical and legal regulation of their use, training of medical personnel in medical bioethics, education of patients and their families.","PeriodicalId":20921,"journal":{"name":"RESEARCH RESULTS IN BIOMEDICINE","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86280101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gastrointestinal stromal tumors in elderly patients","authors":"M. B. Polyanskiy, Sergey P. Dronov, I. N. Zvyagin, Aleksey S. Rukavishnikov","doi":"10.18413/2658-6533-2022-8-1-0-7","DOIUrl":"https://doi.org/10.18413/2658-6533-2022-8-1-0-7","url":null,"abstract":"Background: Gastrointestinal stromal tumors (GISTs) account for 1 to 3% of all primary malignant tumors of the gastrointestinal tract. The global incidence of GISTs varies in the range of 7-15 cases per 1 million people per year. In about 95% of cases, the incidence is sporadic. Despite the fact that some success has been achieved in the treatment of this pathology, the problem of GISTs treatment is urgent, especially in elderly and senile patients in particular. The aim of the study: To study the age-related characteristics of GISTs development in patients of older age groups. Materials and methods: A retrospective analysis of 56 clinical cases of GISTs in patients of different age groups according to the WHO classification was carried out in the study. Results: The most common variant of the immunohistochemical structure was the spindle cell one 62.5%. In most cases, tumors were localized in the stomach 82.2%. Elderly patients had larger tumor sizes compared with young and middle-Aged patients. In patients of older age groups, the disease was most often detected at stage II. In most cases, a comorbid pathology was detected, most often a combination of several diseases of the cardiovascular system. Conclusion: In patients of older age groups, the spindle cell structure of the GISTs is most common, the tumor was most often localized in the stomach (77.4%), most often the tumor was localized along the lesser curvature. In most cases, the tumor was up to 10.0 cm in diameter. On average, the disease was detected at stage II. Comorbid pathology occurred in 87.3% of cases. In 2020-2021, the disease was detected more often, the of tumors sizes were smaller, due to an increase in the number of CT scans of the chest for the diagnosis of the new coronavirus infection. © 2022 AME Publishing Company. All rights reserved.","PeriodicalId":20921,"journal":{"name":"RESEARCH RESULTS IN BIOMEDICINE","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81242901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Studying the role of interlocus interactions of folate cycle genes and matrix metalloproteinases in the formation of fetal growth retardation","authors":"O. A. Efremova","doi":"10.18413/2658-6533-2022-8-1-0-3","DOIUrl":"https://doi.org/10.18413/2658-6533-2022-8-1-0-3","url":null,"abstract":"Background: Fetal growth retardation (FGR) is one of the most common pregnancy complications that can adversely affect the shortand long-term health of newborns. The search for maternal polymorphisms of candidate genes involved in the formation of FGR is important. The aim of the study: To study the role of interlocus interactions of the folate cycle genes and matrix metalloproteinases in the formation of fetal growth retardation. Materials and methods: 477 pregnant women were examined (234 women whose pregnancy was complicated by intrauterine growth retardation and 243 women who had a physiological course of pregnancy (control group)). SNP×SNP interactions of 10 polymorphic loci of matrix metalloproteinase genes and genes affecting the folate cycle associated with the development of FGR were studied using the dimensionality reduction method (MDR method modified by MB-MDR). Validation of the results was carried out using a permutation test (1000 permutations were performed). FGR-related polymorphisms were analyzed in silico for their functional significance. To determine the biological pathways, the following programs were used: Gene Ontology and Genomania. Results: The 7 most significant models of SNP×SNP interactions of matrix metalloproteinase genes and folate cycle genes associated with the development of FGR have been established, which include eight of the 10 SNPs under consideration: rs1805087 MTR, rs1801394 MTRR, rs1979277 SHMT1, rs1799750 MMP-1, rs243865 MMP-2, rs3025058 MMP-3, rs11568819 MMP-7, rs17577 MMP-9 (рperm≤0,05). The largest number of models includes polymorphic loci rs1979277 (7 models), rs243865 (4 models), rs3025058 (3 models). The two-locus combination of TT rs243865 MMP2 x TT rs1979277 SHMT1 genotypes (beta = -0,68, p=0,001) has the most significant association with FGR. These polymorphisms exhibit pronounced functional effects in relation to 38 genes that are involved in the biological pathways of collagen metabolism (mainly in its catabolic breakdown) and modulation of the activity of matrix metalloproteinases (mainly in the processes of increasing the activity of matrix metalloproteinases, causing the breakdown of the extracellular matrix). Conclusion: Interlocus interactions of folate cycle genes and matrix metalloproteinases determine susceptibility to fetal growth retardation.","PeriodicalId":20921,"journal":{"name":"RESEARCH RESULTS IN BIOMEDICINE","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74729191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}