Regulatory Peptides最新文献_第3页

Regulatory Peptides Pub Date : 2014-11-01 DOI: 10.1016/j.regpep.2014.09.003

Bo Ahrén

引用次数: 4

Insulin-like growth factor-1 inhibits colonic smooth muscle cell apoptosis in diabetic rats with colonic dysmotility 胰岛素样生长因子-1抑制糖尿病大鼠结肠平滑肌细胞凋亡

Regulatory Peptides Pub Date : 2014-11-01 DOI: 10.1016/j.regpep.2014.11.005

Manyi Sun , Feng Wang , Ping Feng

引用次数: 4

Expression and release of progalanin in fibroblasts 原丙氨酸在成纤维细胞中的表达和释放

Regulatory Peptides Pub Date : 2014-11-01 DOI: 10.1016/j.regpep.2014.09.004

Hiroyuki Yamamoto , Kazuaki Iguchi , Keiko Unno , Kazuhiko Kaji , Minoru Hoshino

{"title":"Expression and release of progalanin in fibroblasts","authors":"Hiroyuki Yamamoto , Kazuaki Iguchi , Keiko Unno , Kazuhiko Kaji , Minoru Hoshino","doi":"10.1016/j.regpep.2014.09.004","DOIUrl":"10.1016/j.regpep.2014.09.004","url":null,"abstract":"<div><p><span><span><span>Galanin<span> is a neuropeptide expressed in the central and peripheral nervous systems. Galanin is known to be biosynthesized in neural and </span></span>endocrine cells, but little evidence exists for its synthesis in other cells. In this study, we explored galanin-releasing nonneural cells using </span>radioimmunoassay<span>, finding that some fibroblasts produced and released the galanin-like immunoreactive component (galanin-LI). The molecular weight of the galanin-LI obtained from the fibroblasts, as measured by gel filtration chromatography<span> and Western blotting, was 14</span></span></span> <span><span><span><span>kDa and suggested that the compound was progalanin. Peptide mass fingerprinting analysis identified the large form of galanin-LI as progalanin without its </span>signal sequence. In addition, galanin-LI was located in the Golgi bodies and vesicle-like structures of the fibroblasts. Furthermore, the addition of </span>brefeldin A<span>, an inhibitor of transport from the ER, decreased the release of galanin-LI. In this study, we showed that the fibroblast, a nonneural and nonendocrine cell type, produced and released a galanin precursor, progalanin, without processing via Golgi bodies or </span></span>secretory vesicles.</span></p></div>","PeriodicalId":20853,"journal":{"name":"Regulatory Peptides","volume":"194 ","pages":"Pages 55-62"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.regpep.2014.09.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32675111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Mediators involved in the hyperthermic action of neuromedin U in rats 参与大鼠神经质素U热作用的介质

Regulatory Peptides Pub Date : 2014-06-01 DOI: 10.1016/j.regpep.2014.07.004

G. Telegdy , A. Adamik

{"title":"Mediators involved in the hyperthermic action of neuromedin U in rats","authors":"G. Telegdy , A. Adamik","doi":"10.1016/j.regpep.2014.07.004","DOIUrl":"10.1016/j.regpep.2014.07.004","url":null,"abstract":"<div><p><span><span>Neuromedin U (NmU), first was isolated from the porcine spinal cord, has subsequently been demonstrated in a number of species, in which it is present in the periphery and also the brain. Two receptors have been identified: NmU1R is mainly present in peripheral tissues, and Nmu2R in the </span>central nervous system<span>. NmU, a potent endogenous anorectic, serves as a catabolic signaling molecule in the brain; it inhibits food uptake, increases locomotion, activates stress mechanism, having cardiovasscular effects and, causes hyperthermia. The mechanism of this hyperthermia is unknown. In the present experiments, the effects of NmU on the colon temperature following i.c.v administration were studied in rats. For an investigation of the possible role of receptors in mediating hyperthermia, the animals were treated simultaneously with CRF 9–41 and </span></span>antalarmin<span>, a CRH1 receptor inhibitors, astressin 2B, a CRH2 receptor antagonist<span>, haloperidol<span><span> a dopamine receptor antagonist, atropine a muscarinic cholinergic receptor antagonist, noraminophenazone a cyclooxygenase inhibitor or </span>isatin<span>, a prostaglandin receptor antagonist.</span></span></span></span></p><p>NmU increased the colon temperature, maximal action being observed at 2–3h. CRF 9–41, antalarmin, astressin 2B haloperidol, atropine, noraminophenazone and isatin prevented the NmU-induced increase in colon temperature.</p><p><span>The results demonstrated that, when injected into the lateral brain ventricle NmU increased the body temperature, mediated by CRHR1 and CRHR2, dopamine and </span>muscarinic cholinergic receptors. The final pathway involves prostaglandin.</p></div>","PeriodicalId":20853,"journal":{"name":"Regulatory Peptides","volume":"192 ","pages":"Pages 24-29"},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.regpep.2014.07.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32572264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

The ghrelin–GHSR-1a system in the ocular neuro-humoral regulation. Pearls and controversies ghrelin-GHSR-1a系统在眼神经-体液调节中的作用。珍珠与争议

Regulatory Peptides Pub Date : 2014-06-01 DOI: 10.1016/j.regpep.2014.06.001

Amândio Rocha-Sousa

引用次数: 0

Ghrelin and its receptor: The role of the ghrelin signaling system in regulating cardiac function 胃饥饿素及其受体:胃饥饿素信号系统在调节心功能中的作用

Regulatory Peptides Pub Date : 2014-06-01 DOI: 10.1016/j.regpep.2014.08.004

Takeshi Soeki

引用次数: 2

Natriuretic peptides and cerebral hemodynamics 利钠肽与脑血流动力学

Regulatory Peptides Pub Date : 2014-06-01 DOI: 10.1016/j.regpep.2014.07.003

Song Guo , Filippa Barringer , Nora E. Zois , Jens P. Goetze , Messoud Ashina

引用次数: 11

Islet neogenesis-associated protein (INGAP): The role of its endogenous production as a positive modulator of insulin secretion 胰岛新生相关蛋白(INGAP):其内源性生产作为胰岛素分泌的积极调节剂的作用

Regulatory Peptides Pub Date : 2014-06-01 DOI: 10.1016/j.regpep.2014.08.003

Luis E. Flores , Héctor Del Zotto , Florencia Fragapane , Bárbara Maiztegui , Carolina L. Román , Antonio C. Boschero , Juan J. Gagliardino

{"title":"Islet neogenesis-associated protein (INGAP): The role of its endogenous production as a positive modulator of insulin secretion","authors":"Luis E. Flores , Héctor Del Zotto , Florencia Fragapane , Bárbara Maiztegui , Carolina L. Román , Antonio C. Boschero , Juan J. Gagliardino","doi":"10.1016/j.regpep.2014.08.003","DOIUrl":"10.1016/j.regpep.2014.08.003","url":null,"abstract":"<div><p>Islet neogenesis-associated protein (INGAP) is a peptide found in pancreatic exocrine-, duct- and islet- non-β-cells from normal hamsters. Its increase induced by either its exogenous administration or by the overexpression of its gene enhances β-cell secretory function and increases β-cell mass by a combination of stimulation of cell replication and islet neogenesis and reduction of β-cell apoptosis. We studied the potential modulatory role of endogenous INGAP in insulin secretion<span> using two different experimental approaches. Hamster islets transfected with INGAP-small interfering RNA (INGAP-siRNA) were used to study glucose-stimulated insulin secretion (GSIS). In parallel, freshly isolated islets were incubated with high glucose and the same concentration of either a specific anti-INGAP rabbit serum or normal rabbit serum. INGAP-siRNA transfected islets reduced their INGAP mRNA and protein content by 35.1% and 47.2%, respectively whereas GSIS decreased by 25.8%. GSIS by transfected islets attained levels comparable to those recorded in control islets when INGAP pentadecapeptide (INGAP-PP) was added to the culture medium. INGAP antibody in the medium decreased significantly GSIS in a dose-dependent manner. These results indicate that endogenous INGAP plays a “physiological” positive modulatory role in insulin secretion, supporting its possible use in the treatment of prediabetes and Type 2 diabetes.</span></p></div>","PeriodicalId":20853,"journal":{"name":"Regulatory Peptides","volume":"192 ","pages":"Pages 30-34"},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.regpep.2014.08.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32617014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Dual effects of carnosine on energy metabolism of cultured cortical astrocytes under normal and ischemic conditions 肌肽对培养皮层星形胶质细胞正常和缺血状态下能量代谢的双重影响

Regulatory Peptides Pub Date : 2014-06-01 DOI: 10.1016/j.regpep.2014.08.005

Yao Shen, Yueyang Tian, Jianbo Yang, Xiaojie Shi, Li Ouyang, Jieqiong Gao, Jianxin Lu

{"title":"Dual effects of carnosine on energy metabolism of cultured cortical astrocytes under normal and ischemic conditions","authors":"Yao Shen, Yueyang Tian, Jianbo Yang, Xiaojie Shi, Li Ouyang, Jieqiong Gao, Jianxin Lu","doi":"10.1016/j.regpep.2014.08.005","DOIUrl":"10.1016/j.regpep.2014.08.005","url":null,"abstract":"<div><h3>Objective</h3><p>The aim of this study was to investigate the effects of carnosine<span> on the bioenergetic profile of cultured cortical astrocytes under normal and ischemic conditions.</span></p></div><div><h3>Methods</h3><p>The Seahorse Bioscience XF96 Extracellular Flux Analyzer was used to measure the oxygen consumption rates (OCRs) and extracellular acidification rates (ECARs) of cultured cortical astrocytes treated with and without carnosine under normal and ischemic conditions.</p></div><div><h3>Results</h3><p><span>Under the normal growth condition, the basal OCRs and ECARs of astrocytes were 21.72</span>      <span>mpH/min/μg protein respectively. Mitochondrial respiration accounted for ~</span> <span>80% of the total cellular respiration and 85% of this coupled to ATP synthesis. Carnosine significantly reduced basal OCRs and ECARs and ATP-linked respiration, but it strikingly increased the spare respiratory capacity of astrocytes. The cellular ATP level in carnosine-treated astrocytes was reduced to ~</span>   <!-->h of recovery under the normal growth condition.</p></div><div><h3>Conclusions</h3><p>Carnosine may be an endogenous regulator of astrocyte energy metabolism and a clinically safe therapeutic agent for promoting brain energy metabolism recovery after ischemia/reperfusion injury.</p></div>","PeriodicalId":20853,"journal":{"name":"Regulatory Peptides","volume":"192 ","pages":"Pages 45-52"},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.regpep.2014.08.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32646415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Gene expression profiling of gastric mucosa in mice lacking CCK and gastrin receptors 缺乏CCK和胃泌素受体小鼠胃粘膜的基因表达谱

Regulatory Peptides Pub Date : 2014-06-01 DOI: 10.1016/j.regpep.2014.08.002

Chun-Mei Zhao , Yosuke Kodama , Arnar Flatberg , Vidar Beisvag , Bård Kulseng , Arne K. Sandvik , Jens F. Rehfeld , Duan Chen

{"title":"Gene expression profiling of gastric mucosa in mice lacking CCK and gastrin receptors","authors":"Chun-Mei Zhao , Yosuke Kodama , Arnar Flatberg , Vidar Beisvag , Bård Kulseng , Arne K. Sandvik , Jens F. Rehfeld , Duan Chen","doi":"10.1016/j.regpep.2014.08.002","DOIUrl":"10.1016/j.regpep.2014.08.002","url":null,"abstract":"<div><p><span><span>The stomach produces acid, which may play an important role in the regulation of bone homeostasis<span>. The aim of this study was to reveal signaling pathways<span> in the gastric mucosa that involve the </span></span></span>acid secretion and possibly the bone metabolism in CCK</span><sub>1</sub> and/or CCK<sub>2</sub><span> receptor knockout (KO) mice. Gastric acid secretion<span> was impaired and the ECL cell signaling pathway was inhibited in CCK</span></span><sub>2</sub> receptor KO mice but not in CCK<sub>1</sub> receptor KO mice. However, in CCK<sub>1+2</sub><span> receptor double KO mice the acid secretion in response to pylorus ligation-induced vagal stimulation and the ECL cell pathway were partially normalized, which was associated with an up-regulated pituitary adenylate cyclase-activating polypeptide (PACAP) type 1 receptor (PAC1). The basal part of the gastric mucosa expressed parathyroid hormone-like hormone (PTHLH) in a subpopulation of likely ECL cells (and possibly other cells) and vitamin D3<span> 1α hydroxylase<span><span> probably in trefoil peptide2-immunoreactive cells. In conclusion, mice lacking CCK receptors exhibited a functional shift from the gastrin-CCK pathways to the neuronal pathway in control of the ECL cells and eventually the acid secretion. Taking the present data together with previous findings, we suggest a possible link between gastric PTHLH and </span>vitamin D and bone metabolism.</span></span></span></p></div>","PeriodicalId":20853,"journal":{"name":"Regulatory Peptides","volume":"192 ","pages":"Pages 35-44"},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.regpep.2014.08.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32617585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6