Dual effects of carnosine on energy metabolism of cultured cortical astrocytes under normal and ischemic conditions

Regulatory Peptides Pub Date : 2014-06-01 DOI:10.1016/j.regpep.2014.08.005

Yao Shen, Yueyang Tian, Jianbo Yang, Xiaojie Shi, Li Ouyang, Jieqiong Gao, Jianxin Lu

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引用次数: 18

Abstract

Objective

The aim of this study was to investigate the effects of carnosine on the bioenergetic profile of cultured cortical astrocytes under normal and ischemic conditions.

Methods

The Seahorse Bioscience XF96 Extracellular Flux Analyzer was used to measure the oxygen consumption rates (OCRs) and extracellular acidification rates (ECARs) of cultured cortical astrocytes treated with and without carnosine under normal and ischemic conditions.

Results

Under the normal growth condition, the basal OCRs and ECARs of astrocytes were 21.72 ± 1.59 pmol/min/μg protein and 3.95 ± 0.28 mpH/min/μg protein respectively. Mitochondrial respiration accounted for ~ 80% of the total cellular respiration and 85% of this coupled to ATP synthesis. Carnosine significantly reduced basal OCRs and ECARs and ATP-linked respiration, but it strikingly increased the spare respiratory capacity of astrocytes. The cellular ATP level in carnosine-treated astrocytes was reduced to ~ 42% of the control. However, under the ischemic condition, carnosine upregulated the mitochondrial respiratory and cellular ATP content of astrocytes exposed to 8 h of oxygen–glucose deprivation (OGD) followed by 24 h of recovery under the normal growth condition.

Conclusions

Carnosine may be an endogenous regulator of astrocyte energy metabolism and a clinically safe therapeutic agent for promoting brain energy metabolism recovery after ischemia/reperfusion injury.

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肌肽对培养皮层星形胶质细胞正常和缺血状态下能量代谢的双重影响

目的探讨肌肽对正常和缺血条件下培养的皮质星形胶质细胞生物能量谱的影响。方法采用海马Bioscience XF96细胞外通量分析仪测定经肌肽处理和不经肌肽处理的皮质星形胶质细胞在正常和缺血状态下的耗氧量(OCRs)和细胞外酸化率(ECARs)。结果在正常生长条件下，星形胶质细胞的基础ocr和ecar分别为21.72±1.59 pmol/min/μg蛋白和3.95±0.28 mpH/min/μg蛋白。线粒体呼吸约占细胞呼吸总量的80%，其中85%与ATP合成有关。肌肽显著降低基础ocr和ecar以及atp相关呼吸，但显著增加星形胶质细胞的备用呼吸能力。肌肽处理的星形胶质细胞的细胞ATP水平降低到对照组的42%。然而，在缺血条件下，肌肽上调了8 h氧葡萄糖剥夺(OGD)和24 h正常生长条件下恢复的星形胶质细胞的线粒体呼吸和细胞ATP含量。结论肌肽可能是星形胶质细胞能量代谢的内源性调节剂，是促进缺血再灌注损伤后脑组织能量代谢恢复的临床安全的治疗药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Regulatory Peptides 医学-内分泌学与代谢

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： Regulatory Peptides provides a medium for the rapid publication of interdisciplinary studies on the physiology and pathology of peptides of the gut, endocrine and nervous systems which regulate cell or tissue function. Articles emphasizing these objectives may be based on either fundamental or clinical observations obtained through the disciplines of morphology, cytochemistry, biochemistry, physiology, pathology, pharmacology or psychology.