Rafidain journal of science最新文献_第6页

Biosynthesis of Iron Oxide Nanoparticles and Using it to Support the Efficiency of Wastewater Filtration System 氧化铁纳米颗粒的生物合成及其在污水过滤系统中的应用

Rafidain journal of science Pub Date : 2023-03-01 DOI: 10.33899/rjs.2023.177285

M. Al-Allaf, A. AL-Rawi, Kossay AL-Ahmadi

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Estimation of the Levels of some Immunological Markers in Aborted Women Infected with Toxoplasma gondii at Baghdad City 巴格达市刚地弓形虫感染流产妇女某些免疫标志物水平的测定

Rafidain journal of science Pub Date : 2023-03-01 DOI: 10.33899/rjs.2023.177286

Sanaa Ahmed, H. Khalaf, N. Jafar, B. Noomi

{"title":"Estimation of the Levels of some Immunological Markers in Aborted Women Infected with Toxoplasma gondii at Baghdad City","authors":"Sanaa Ahmed, H. Khalaf, N. Jafar, B. Noomi","doi":"10.33899/rjs.2023.177286","DOIUrl":"https://doi.org/10.33899/rjs.2023.177286","url":null,"abstract":"Toxoplasma gondii is one of the most common parasites among humans worldwide, as serological studies indicate that more than one third of the population of the world is infected with this parasite. This parasite is one of the main causes of abortion in pregnant women which mainly occur in the acute phase of infection and in early pregnancy. The present study aimed to detect toxoplasma among pregnant women and its relationship with some immunological markers. Fifty blood samples (5ml) were collected from aborted woman (within 15 days after abortion) and 10 blood samples collected from healthy woman as control. The serums were used to evaluate IL-8, IL-10, IL-17, and IFN-γ. The results showed that toxoplasma caused abortion in the first trimester in rate of 68%. The majority of aborted women (76%) were at age group 25-30 years. As for immunological parameters, IL-8, IL-10, IL-17, and IFN-γ significantly increased in woman aborted with toxoplasmosis in compare with healthy woman. The parameters were also higher among toxoplasma positive patients when compared with aborted women with other cause but these increases in the immunological parameters were not significant. The study conclude that toxoplasma is still the main causes of abortion in women and it associated with significant increase in immunological markers represented by IL-8, IL-10, IL-17, and IFN-γ.","PeriodicalId":20803,"journal":{"name":"Rafidain journal of science","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86178206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis and Spectral Study of some New α, β-Unsaturated Carbonyl Compounds and Pyrazole Derivatives 一些新的α、β-不饱和羰基化合物及吡唑衍生物的合成与光谱研究

Rafidain journal of science Pub Date : 2023-03-01 DOI: 10.33899/rjs.2023.177296

R. Hassan, A. Al-Sabawi

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Isolation and Identification of some Yeasts from some Plants 几种植物酵母菌的分离与鉴定

Rafidain journal of science Pub Date : 2023-03-01 DOI: 10.33899/rjs.2023.177284

Maha Al-Taei, Badia Malla Obaeda

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Isolation and Identification of Xylella fastidiosa that Cause Oleander Leaf Scorch 夹竹桃枯叶木杆菌的分离与鉴定

Rafidain journal of science Pub Date : 2023-03-01 DOI: 10.33899/rjs.2023.177292

Muhammed Z. Bakir, Khalid M. Ahmed, Ramadan Y. Mohamed

{"title":"Isolation and Identification of Xylella fastidiosa that Cause Oleander Leaf Scorch","authors":"Muhammed Z. Bakir, Khalid M. Ahmed, Ramadan Y. Mohamed","doi":"10.33899/rjs.2023.177292","DOIUrl":"https://doi.org/10.33899/rjs.2023.177292","url":null,"abstract":"During spring of 2022, leaf scorch symptom was observed in Nerium oleander plant in Erbil city. Surveys were carried out in three main Erbil streets (60-meter, 120 meter and Qasmlo) to determine the occurrence and distribution of oleander leaf scorch. Oleander leaf scorch (OLS) is a disease caused by xylem limited bacterium Xylella fastidiosa . The percentage of plants with Oleander leaf scorch infection ranged usually between 23-91%, and the disease was assessed the highest disease rate based on disease rating scale was recorded in 60-meter street is 91.95%, Qasmlo street is 89.07% and the lowest disease rate was observed in 120-meter street is 23.17%. In this study the bacterium was detected after isolated in laboratory as Gram stain test was performed and the isolated bacterium was Gram negative, rod shaped, non-flagellate and non-motile also identified by PCR were performed with two primers forward primer 16S (AGAG TTTG ATCC TGGC TCAG) and reverse primer 16S (GGCT ACCT TGTT ACGA CTT), amplified a 1200 bp fragment from the isolated OLS. The Analysis of BLAST revealed that the highest identity number query sequences were (100%) identified and submitted in GenBank and have taken accession number Xylella fastidiosa subsp. Sandyi (ON131099) and Xylella fastidiosa subsp. Sandyi (ON131100) also the phylogenetic results show the each identified bacteria are grouped in one clade with other sequences were stored in NCBI GenBank. This is a first report of a „ Xylella fastidiosa .in Erbil city.","PeriodicalId":20803,"journal":{"name":"Rafidain journal of science","volume":"97 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87227119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Family Specialty for Microbacterium sp. AJ-Z Isolated from Fenugreek (Trigonella foenum-graecum) Root Nodules 胡芦巴根瘤中微细菌sp. j - z的家族特征检测

Rafidain journal of science Pub Date : 2023-03-01 DOI: 10.33899/rjs.2023.177283

Z. Qaddawi, A. Mohammed, William O. Onu

引用次数: 0

Synthesis, Characterization of Substituted 1,3-Oxazepine, Thiazolidine-4-one and Azetidine-2-one Using Benzimidazole as a Synthon 以苯并咪唑为合成物的取代1,3-恶氮平、噻唑烷-4- 1和氮杂啶-2- 1的合成与表征

Rafidain journal of science Pub Date : 2023-03-01 DOI: 10.33899/rjs.2023.177293

Muhammed O. Muhammed

引用次数: 0

Green Synthesis of Silver Nanoparticles Using Nigella sativa Callus and Cellular Suspension Cultures with Different pH Values 用不同pH值的黑草愈伤组织和细胞悬浮培养绿色合成纳米银

Rafidain journal of science Pub Date : 2023-03-01 DOI: 10.33899/rjs.2023.177290

Sawsan J. A. AL-Allaf, Rehab Albaker

引用次数: 0

Biodiversity of some Species of Alternaria Fungi Causing Spotting in Ornamental Plants 观赏植物中引起斑点病的几种互交菌的生物多样性

Rafidain journal of science Pub Date : 2023-03-01 DOI: 10.33899/rjs.2023.177291

Noor Al-healy, Warka Qassim

引用次数: 0

Screening of Conditions for Manganese Peroxidase Production from White Rot Fungi (Pleurotus djamor) 白腐菌(Pleurotus djamor)产锰过氧化物酶条件的筛选

Rafidain journal of science Pub Date : 2023-01-01 DOI: 10.33899/rjs.2023.178571

E. G, E. I., Oparaji H.

{"title":"Screening of Conditions for Manganese Peroxidase Production from White Rot Fungi (Pleurotus djamor)","authors":"E. G, E. I., Oparaji H.","doi":"10.33899/rjs.2023.178571","DOIUrl":"https://doi.org/10.33899/rjs.2023.178571","url":null,"abstract":"The need for alternative and environmental friendly methods of waste clean-up has led to the use of enzymes in bioremediation. In this study, white rot fungi were isolated from decaying plant parts using standard microbiology and biochemical techniques. The isolated fungal mycelial were identified and screened using standard substrates (2, 6-DMP) to determine their capability for production of Manganese peroxidase. Pure manganese peroxidase was achieved after four distinct purification phases. Crude extract of the homogenate proteins obtained through optimized submerged fermentation system were precipitated using ammonium sulphate salt. Ammonium sulphate of 60% concentrated at pH 4.5 precipitated protein with highest Manganese peroxidase activity (322 U/mg). The precipitated proteins was desalted through dialysis for twelve hours (12hrs) with buffer exchange at interval of six hours (6 hrs) and activity of 343.91 U/mg was recorded afterwards. DEAE-cellulose was used for the ion exchange purification of the dialyzed protein, salt (NaCl) gradients of 0.3-0.6 M was found best in washing off the bound protein from the exchange resin and activity of 434.18 U/mg was recorded from the pooled fraction tubes. sephadex G-100 was used for separation of the proteins into molecular sizes and weights. 2.8 purification folds of the enzyme were gotten after ion exchange (DEAE-cellulose) and gel filtration (sephadex G-100) with percentage yield of 2.20%. Specific activity of the enzyme increased to 602.00% after gel filtration. The partial purified enzyme was further characterized for determination of optimal pH (4.5), temperature (40 °C) and kinetic properties K M of 3.4 mM and V MAX of 250 µmol/min.","PeriodicalId":20803,"journal":{"name":"Rafidain journal of science","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77254473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0