Radiation BotanyPub Date : 1974-12-01DOI: 10.1016/S0033-7560(74)80010-4
William R. Jordan III , Alan H. Haber
{"title":"Cytokinins and mitotic inhibition in “gamma-plantlets”","authors":"William R. Jordan III , Alan H. Haber","doi":"10.1016/S0033-7560(74)80010-4","DOIUrl":"https://doi.org/10.1016/S0033-7560(74)80010-4","url":null,"abstract":"<div><p>After irradiation of dry grains with 500 krads of gamma rays, the cytokinin content of wheat seedlings germinating without cell division rose from a barely detectable quantity in the dry grains to an amount similar to that in seedlings from unirradiated controls. The chromatographic mobility of the cytokinins from the two sources suggests that they are identical. Treatment of the “gamma-plantlets” with cytokinins and several combinations of other growth substances failed to induce cell division. It is concluded that accumulation of extractable cytokinins can be uncoupled from cell division and that failure of cell division in gamma-plantlets is not due to a gross deficiency of extractable cytokinins.</p></div>","PeriodicalId":20794,"journal":{"name":"Radiation Botany","volume":"14 4","pages":"Pages 219-222"},"PeriodicalIF":0.0,"publicationDate":"1974-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0033-7560(74)80010-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91741523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Radiation BotanyPub Date : 1974-12-01DOI: 10.1016/S0033-7560(74)80012-8
Harvey L. Ragsdale, W.A. Rhoads
{"title":"Four-year post-exposure assay of vegetation surrounding project pinstripe: Demonstration of the utility of delayed damage appraisals","authors":"Harvey L. Ragsdale, W.A. Rhoads","doi":"10.1016/S0033-7560(74)80012-8","DOIUrl":"10.1016/S0033-7560(74)80012-8","url":null,"abstract":"<div><p>This report illustrates the feasibility of using temporally-delayed vegetation assays to determine radiation damage, by documenting the radiation damage resulting from the accidental venting of radioactive materials during Project Pinstripe, Frenchman's Flat, Nevada Test Site, in April, 1966. Evidence of desert shrub radiation damage was first observed and photographed, in April, 1968. Systematic study of the vegetation was initiated in October, 1970, and evidence of radiation damage documented over 72·9 hectares adjacent to the vent. Beta doses were estimated at 15–21 krads based on gamma exposure dose measurements. The minimum beta dose estimate was substantially greater than the theoretical lethal dose for the shrub, <em>Larrea divaricata</em>. Radiation damage to the shrubs, <em>Larrea divaricata, Ephedra funerea</em>, and <em>Atriplex confertifolia</em> was expressed as differential bud mortality, partial death of shrub crowns with and without crown regrowth, and total shrub crown death without crown regrowth. Each of the shrub populations was statistically different from its control population with respect to the distribution of individuals among damage classes. Generally, damage patterns were similar to those observed at two previously-studied Plowshare events.</p></div>","PeriodicalId":20794,"journal":{"name":"Radiation Botany","volume":"14 4","pages":"Pages 229-230, IN1-IN2, 231-236"},"PeriodicalIF":0.0,"publicationDate":"1974-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0033-7560(74)80012-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83150656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Radiation BotanyPub Date : 1974-12-01DOI: 10.1016/S0033-7560(74)80029-3
C.B. Singh
{"title":"In vivo labelling of nucleic acids during microsporogenesis in tomato","authors":"C.B. Singh","doi":"10.1016/S0033-7560(74)80029-3","DOIUrl":"https://doi.org/10.1016/S0033-7560(74)80029-3","url":null,"abstract":"<div><p>Methods are described for administering tritiated thymidine and orotic acid to microsporocytes of tomato at early meiotic prophase—I. Of five methods examined, bud inoculation proved to be the most effective for efficient entry and incorporation of precursors and convenience in handling a large population with least disturbance to the meiotic system. Application of this technique could be extended to other plant species having a similar growth habit and floral morphology.</p></div>","PeriodicalId":20794,"journal":{"name":"Radiation Botany","volume":"14 4","pages":"Pages 355-358"},"PeriodicalIF":0.0,"publicationDate":"1974-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0033-7560(74)80029-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91741790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Radiation BotanyPub Date : 1974-12-01DOI: 10.1016/S0033-7560(74)80027-X
A. Arain
{"title":"The effect of ethyl methanesulfonate treatment on floret sterility and chlorophyll mutation rate in barley","authors":"A. Arain","doi":"10.1016/S0033-7560(74)80027-X","DOIUrl":"https://doi.org/10.1016/S0033-7560(74)80027-X","url":null,"abstract":"","PeriodicalId":20794,"journal":{"name":"Radiation Botany","volume":"13 1","pages":"347-350"},"PeriodicalIF":0.0,"publicationDate":"1974-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76677263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Radiation BotanyPub Date : 1974-12-01DOI: 10.1016/S0033-7560(74)80017-7
Edward Flaccus , Thomas V. Armentano, Marcie Archer
{"title":"Effects of chronic gamma radiation on the composition of the herb community of an oak-pine forest","authors":"Edward Flaccus , Thomas V. Armentano, Marcie Archer","doi":"10.1016/S0033-7560(74)80017-7","DOIUrl":"https://doi.org/10.1016/S0033-7560(74)80017-7","url":null,"abstract":"<div><p>A complete inventory of the herb populations of the inner 50 m of the Brookhaven irradiated forest was made in 1968 and 1973. The data are compared with those of earlier inventories, giving information on changes through a ten-year period in a community exposed to chronic radiation stress.</p><p>Per cent cover of <em>Carex pensyluanica</em>, which dominates the zone 20–40 m from the source (125-25 R/day in 1973), increased rapidly at first and then more slowly through 1968, then declined somewhat by 1973. There was no shift in its distribution over ten years. Sharp increase in <em>Rubus</em> spp. cover in this zone contributed to the most recent <em>Carex</em> decline.</p><p>A greater than eight-fold rise in total density of species other than <em>Carex</em> occurred since 1968, mostly due to a large increase in density of important species, while only those of infrequent occurrence declined or disappeared by 1973. Rate of change in herb populations was slowed by high exposures close to the source and by competition with <em>Carex</em> and <em>Rubus</em> in the sedge zone. The distribution of <em>Rumex acetosella</em> changed in a manner suggesting possible genetic alteration of radiosensitivity. There was a net increase of four species between 1968 and 1973.</p><p>Species diversity increased with distance from the source, increased with time from 1968 to 1973 in the outer 40–50 m zone, and changed relatively slowly with time in the inner zones of higher exposure rates. The reasons for the comparatively slow rates of successional change are discussed.</p><p>We predict that with future reduction in radiation stress due to source decay and with continuing expansion of <em>Rubus</em> spp. clones, <em>Carex</em> dominance will continue to decline as species diversity and herb population densities increase slowly.</p></div>","PeriodicalId":20794,"journal":{"name":"Radiation Botany","volume":"14 4","pages":"Pages 263-271"},"PeriodicalIF":0.0,"publicationDate":"1974-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0033-7560(74)80017-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91741442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Radiation BotanyPub Date : 1974-12-01DOI: 10.1016/S0033-7560(74)80020-7
J.P. Skou, K. Bech, Kirsten Lundsten
{"title":"Effects of ionizing irradiation on mushrooms as influenced by physiological and environmental conditions","authors":"J.P. Skou, K. Bech, Kirsten Lundsten","doi":"10.1016/S0033-7560(74)80020-7","DOIUrl":"https://doi.org/10.1016/S0033-7560(74)80020-7","url":null,"abstract":"<div><p>The effects of irradiation with β (10 MeV fast electrons)- and γ-rays were studied on several characters in strains of the cultured mushroom under different physiological and environmental conditions, including uncut and cut mushrooms, tightness of packing, and relative humidity. Weight loss was greatest in the non-irradiated mushrooms owing to evaporation from an increased surface area resulting from expansion and ripening which were greatly retarded in the irradiated samples. Twenty-five krads of β- or γ-rays had a significant, but transitory, effect on the veil opening. The inhibition became long-lasting and improved with increasing dose. High relative humidity and free exchange of gases were essential for maintaining quality. Depending on the conditions, expansion of pilei and opening of veils were affected differently by irradiation with β- and γ-rays. The strains displayed different opening rates. Expansion and elongation were retarded significantly by 100 krads. The effect improved further with increasing dose. Irradiation improved the skin colour when the mushrooms were stored uncovered or in boxes with perforated PVC-foil. The opposite was the case when the boxes were sealed. The colour of the flesh was examined by measuring its reflectance. Edaphic factors or age of the culture (flush-number) influenced the slope of the reflectance curves. Generally, irradiation increased flesh discoloration, but more in the stipes than in pilei. This adverse effect would make acceptance of irradiated mushrooms by consumers questionable.</p><p>The flesh of the pilei was almost unaffected by the packing, while packing gave a positive response on the stipes. Surface mould fluff was reduced by 50 krads of β- or γ-rays and almost extinct after 200-krad treatment. Growth of pathogens such as <em>Pseudomonas tolaasii</em> and <em>Mycogone pemiciosa</em> was prevented by 200 krads.</p></div>","PeriodicalId":20794,"journal":{"name":"Radiation Botany","volume":"14 4","pages":"Pages 287-288, IN5-IN6, 289-299"},"PeriodicalIF":0.0,"publicationDate":"1974-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0033-7560(74)80020-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91741445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Radiation BotanyPub Date : 1974-12-01DOI: 10.1016/S0033-7560(74)80018-9
R.K. Schulz, J. Ulrich, K.L. Babcock
{"title":"Effect of simulated dew on fallout retention and beta radiation damage to a bean crop","authors":"R.K. Schulz, J. Ulrich, K.L. Babcock","doi":"10.1016/S0033-7560(74)80018-9","DOIUrl":"https://doi.org/10.1016/S0033-7560(74)80018-9","url":null,"abstract":"<div><p>A beta-emitting fallout simulant (very fine sand labeled with varying levels of <sup>90</sup>Y) was applied to a field bean crop <em>Phaseolus vulgaris</em> L.). The foliage was wet or dry in alternate rows at time of application, thus simulating presence or absence of dew conditions. The presence of moisture caused a much larger initial retention of the simulant by the foliage. Dosimetry indicated that the contact dose under moist conditions was substantial, with the wet plants receiving a total dose 60 per cent greater than the dry plants. The yield of string beans was very sensitive to beta radiation and a dose of about 1000 rads to the surface of the apical buds severely reduced the string bean yield. It was estimated that this corresponded to a dose of about 400 rads to the meristematic tissue of these buds. Vegetative growth was much more resistant to the radiation with serious vegetative yield reductions occurring at about 10,000 rads to the surface of the apical buds.</p></div>","PeriodicalId":20794,"journal":{"name":"Radiation Botany","volume":"14 4","pages":"Pages 273-276, IN3, 277-279"},"PeriodicalIF":0.0,"publicationDate":"1974-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0033-7560(74)80018-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91741526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Radiation BotanyPub Date : 1974-12-01DOI: 10.1016/S0033-7560(74)80016-5
R. S. Kahan
{"title":"Accelerated and increased development of inflorescences in tomato plants from irradiated seeds","authors":"R. S. Kahan","doi":"10.1016/S0033-7560(74)80016-5","DOIUrl":"https://doi.org/10.1016/S0033-7560(74)80016-5","url":null,"abstract":"","PeriodicalId":20794,"journal":{"name":"Radiation Botany","volume":"17 1","pages":"257-262"},"PeriodicalIF":0.0,"publicationDate":"1974-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89724563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Radiation BotanyPub Date : 1974-12-01DOI: 10.1016/S0033-7560(74)80019-0
G. P. Howland, E. L. Boyd
{"title":"Genetic control of photomorphogenesis. Isolation of nonfilamentous mutants after gamma irradiation of Pteridium aquilinum spores","authors":"G. P. Howland, E. L. Boyd","doi":"10.1016/S0033-7560(74)80019-0","DOIUrl":"https://doi.org/10.1016/S0033-7560(74)80019-0","url":null,"abstract":"","PeriodicalId":20794,"journal":{"name":"Radiation Botany","volume":"8 1","pages":"281-285"},"PeriodicalIF":0.0,"publicationDate":"1974-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87533488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}