Protein expression and purification最新文献_第2页

Purification, folding, activity analysis and substrate specificity of Pseudomonas diacylglycerol kinase 假单胞菌二酰基甘油激酶的纯化、折叠、活性分析和底物特异性

IF 1.4 4区生物学

Protein expression and purification Pub Date : 2025-04-27 DOI: 10.1016/j.pep.2025.106723

Yipeng Chen , Bin Huai , Jin Chuan Wu , Ning Zhang , Yong Wang , Qingxin Li

{"title":"Purification, folding, activity analysis and substrate specificity of Pseudomonas diacylglycerol kinase","authors":"Yipeng Chen , Bin Huai , Jin Chuan Wu , Ning Zhang , Yong Wang , Qingxin Li","doi":"10.1016/j.pep.2025.106723","DOIUrl":"10.1016/j.pep.2025.106723","url":null,"abstract":"<div><div>The structural and functional investigation of bacterial membrane proteins is crucial to the development of antibiotics. Diacylglycerol kinase (DAGK) from <em>Escherichia coli</em> (<em>E. coli</em>) has been extensively studied as a model membrane protein. However, the DAGK from <em>Pseudomonas aeruginosa</em> (PAO1-DAGK) with a 44 % sequence identity to <em>E. coli</em>-DAGK is not well characterized. To explore the properties of PAO1-DAGK, it was successfully expressed in <em>E. coli</em> and was purified in Decyl-β-D-maltoside (DM) micelles followed with characterizations. Chemical cross-linking studies revealed that PAO1-DAGK in DM micelles could form dimers and trimers. The kinase activity of PAO1-DAGK was determined to be 24.2 ± 2.2 U/mg protein in a mixed-micelle system. The effects of pH and temperature on the activity of PAO1-DAGK were also investigated, respectively. PAO1-DAGK in DM micelles exhibited good stability at pH 6.0–10.0 and below 45 °C. Substrate specificity measurements indicated that PAO1-DAGK demonstrated a clear preference for medium-chain diacylglycerols (DAGs) in the mixed-micelle system, with sn-1,2-Dihexanoylglycerol (DiC6) being the most favored substrate. Molecular docking results demonstrated the interactions between DAGs and PAO1-DAGK.</div></div>","PeriodicalId":20757,"journal":{"name":"Protein expression and purification","volume":"232 ","pages":"Article 106723"},"PeriodicalIF":1.4,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced production of functional CRISPR-AsCas12a protein in Escherichia coli 在大肠杆菌中增强功能性CRISPR-AsCas12a蛋白的产生

IF 1.4 4区生物学

Protein expression and purification Pub Date : 2025-04-25 DOI: 10.1016/j.pep.2025.106722

Orlando S. Goméz-Quintero, Melissa D. Morales-Moreno, Erick G. Valdés-Galindo, Rosa E. Cárdenas-Guerra, Armando Hernandez-Garcia

{"title":"Enhanced production of functional CRISPR-AsCas12a protein in Escherichia coli","authors":"Orlando S. Goméz-Quintero, Melissa D. Morales-Moreno, Erick G. Valdés-Galindo, Rosa E. Cárdenas-Guerra, Armando Hernandez-Garcia","doi":"10.1016/j.pep.2025.106722","DOIUrl":"10.1016/j.pep.2025.106722","url":null,"abstract":"<div><div>The CRISPR-Cas12a system is a groundbreaking tool widely used for genome editing and diagnostics in biotechnology and biomedicine research laboratories. Despite its growing application, studies optimizing Cas12a protein production at the laboratory scale using straightforward protocols remains scarce. This study aimed to enhance the lab-scale recombinant production of <em>Acidaminococcus</em> sp Cas12a protein (AsCas12a) in <em>E. coli</em>. Through targeted adjustments of simple parameters, AsCas12a production was significantly increased. The optimized conditions included the use of <em>E. coli</em> BL21(DE3), TB medium supplemented with 1 % glucose, induction with 0.3 mM IPTG for at least 6–9 h, and incubation at 30 °C. Notably, these conditions differ from conventional protocols typically used for Cas12a and related proteins, such as <em>Streptococcus pyogenes</em> Cas9. Upon combining all optimized parameters, AsCas12a production increased approximately 3-fold, from 0.95 mg/mL of bacterial lysate under non-optimized conditions to 3.73 mg/mL under optimized ones. After chromatographic purification, the final protein yield rose approximately 4.5-fold, from 5.2 to 23.4 mg/L of culture volume, without compromising functional activity. The purified AsCas12a retained full activity for programmable <em>in vitro</em> DNA <em>cis</em>-cleavage and collateral <em>trans</em>-cleavage, which was successfully applied to detect the N gene of SARS-CoV-2. This optimized method provide an efficient and high-yield approach for producing functional AsCas12a protein using accessible materials and conditions available to many research laboratories worldwide.</div></div>","PeriodicalId":20757,"journal":{"name":"Protein expression and purification","volume":"232 ","pages":"Article 106722"},"PeriodicalIF":1.4,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143890637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A recombinant human SLPI variant suppresses the formation of neutrophil extracellular traps at low concentrations in vitro 重组人SLPI变体在体外低浓度抑制中性粒细胞胞外陷阱的形成

IF 1.4 4区生物学

Protein expression and purification Pub Date : 2025-04-23 DOI: 10.1016/j.pep.2025.106721

Felipe de Jesus Gonzalez-Contreras , Roxana Guadalupe Gutierrez-Vidal , Xristo Zarate

{"title":"A recombinant human SLPI variant suppresses the formation of neutrophil extracellular traps at low concentrations in vitro","authors":"Felipe de Jesus Gonzalez-Contreras , Roxana Guadalupe Gutierrez-Vidal , Xristo Zarate","doi":"10.1016/j.pep.2025.106721","DOIUrl":"10.1016/j.pep.2025.106721","url":null,"abstract":"<div><div>Neutrophil extracellular traps (NETs) are web-like structures released by neutrophils to trap and kill microbes. While NETs are crucial in host defense, excessive formation can lead to autoimmune diseases. Currently, no specific drugs target NETs directly; however, some medications can indirectly modulate their formation. The secretory leukocyte protease inhibitor (SLPI) is a small protein that inhibits the activity of neutrophil elastase (NE), an enzyme essential for NETs formation. By inhibiting NE activity, SLPI prevents the chromatin from decondensing, which is necessary for NETs to form; this suggests that SLPI may protect by preventing excessive NETs development. However, evidence indicates that NE can inactivate SLPI by cleaving its N-terminus. This action can create a protease/antiprotease imbalance, potentially leading to detrimental consequences for the host. In this study, we produced a recombinant variant of SLPI (SLPI-S15G-A16G: rSLPIv) using recombinant DNA technology, expressed in <em>Escherichia coli</em> SHuffle T7. The protein was tagged with the small metal-binding protein (SmbP) to facilitate its expression and purification through immobilized metal-affinity chromatography. Our results demonstrated that rSLPIv exhibited immunomodulatory activity at a concentration of 10 nM in neutrophils that had been prestimulated with PMA. It reduced NETs formation by 30 % and maintained this effect for up to 6 h. Confocal microscopy confirmed these findings, revealing a rSLPIv-dependent reduction in neutrophil nuclear expansion. Thus, rSLPIv shows significant suppressive activity on NETs formation at low concentrations, making it a potential candidate as an immunotherapeutic agent.</div></div>","PeriodicalId":20757,"journal":{"name":"Protein expression and purification","volume":"232 ","pages":"Article 106721"},"PeriodicalIF":1.4,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical characterization and molecular dynamics study of a thermostable endo-1,5-α-arabinanase 耐热内源性1,5-α-阿拉伯糖酶的生化表征及分子动力学研究

IF 1.4 4区生物学

Protein expression and purification Pub Date : 2025-04-19 DOI: 10.1016/j.pep.2025.106720

Vandierly Sampaio de Melo , Brisa Moreira Gomes , Tarcillo Gaziri , Elvira Regina Tamarozzi , Felipe Santiago Chambergo

{"title":"Biochemical characterization and molecular dynamics study of a thermostable endo-1,5-α-arabinanase","authors":"Vandierly Sampaio de Melo , Brisa Moreira Gomes , Tarcillo Gaziri , Elvira Regina Tamarozzi , Felipe Santiago Chambergo","doi":"10.1016/j.pep.2025.106720","DOIUrl":"10.1016/j.pep.2025.106720","url":null,"abstract":"<div><div>Arabinan, a crucial constituent of plant biomass, undergoes hydrolysis through the enzymes endo-1,5-α-L-arabinanases and α-L-arabinofuranosidases, resulting in the release of L-arabinose. This monosaccharide holds diverse applications in the biofuel, food, and pharmaceutical industries. In this study, we characterized GeoARA, a recombinant enzyme of endo-1,5-α-L-arabinanase from <em>Geobacillus</em> sp. JS12, previously undescribed in this organism. GeoARA, expressed in <em>E. coli</em> BL21 and purified via affinity chromatography, displayed optimal activity at pH 7.0 and temperature of 70 °C on the specific substrate debranched arabinan. In the presence of metallic ions and EDTA as additives, the enzyme demonstrated high stability. Notably, the enzyme maintained high thermal stability at 70°C for up to 48 h, with enhanced performance in the presence of Co<sup>2+</sup>. GeoARA demonstrated a specific activity on debranched arabinan of 226.7 U mg<sup>−1</sup>, with K<sub>m</sub>, V<sub>max</sub>, k<sub>cat</sub>, and k<sub>cat</sub>/K<sub>m</sub> values of 12.1 mg mL<sup>−1</sup>, 1284.7 μmol min<sup>−1</sup> mg<sup>−1</sup>, 642.3 s<sup>−1</sup>, and 53 mL mg<sup>−1</sup> s<sup>−1</sup>, respectively. Furthermore, the three-dimensional structure of GeoARA was determined using homology-based molecular modeling, and the predicted model quality was validated through molecular dynamics simulations. The integration of computational and biochemical analyses confirmed that the enhanced thermostability observed experimentally, particularly in the presence of the cobalt ion, is associated with reduced atomic fluctuations and increased structural rigidity near the catalytic site. Together, these biochemical features position the endo-1,5-α-L-arabinanase GeoARA as a promising candidate for efficiently extracting L-arabinose in industrial applications.</div></div>","PeriodicalId":20757,"journal":{"name":"Protein expression and purification","volume":"231 ","pages":"Article 106720"},"PeriodicalIF":1.4,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143852336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression, purification, and enzymatic characterization of human UDP-glucose:glycoprotein glucosyltransferase-selenoprotein F complex from Sf9 insect cells Sf9昆虫细胞中人udp -葡萄糖糖蛋白-葡萄糖基转移酶-硒蛋白F复合物的表达、纯化和酶学特性

IF 1.4 4区生物学

Protein expression and purification Pub Date : 2025-04-16 DOI: 10.1016/j.pep.2025.106719

Hirotaka Tomii , Takashi Kikuma , Hiroyuki Kajiura , Kanae Sano , Hiroyoshi Matsumura , Yukishige Ito , Yasuhiro Kajihara , Yoichi Takeda

{"title":"Expression, purification, and enzymatic characterization of human UDP-glucose:glycoprotein glucosyltransferase-selenoprotein F complex from Sf9 insect cells","authors":"Hirotaka Tomii , Takashi Kikuma , Hiroyuki Kajiura , Kanae Sano , Hiroyoshi Matsumura , Yukishige Ito , Yasuhiro Kajihara , Yoichi Takeda","doi":"10.1016/j.pep.2025.106719","DOIUrl":"10.1016/j.pep.2025.106719","url":null,"abstract":"<div><div>Appropriate functioning of the glycoprotein quality control system in the endoplasmic reticulum is crucial for maintaining cellular homeostasis. UDP-glucose:glycoprotein glucosyltransferase 1 (UGGT1) is a key component of this system, serving as a “folding sensor\" by recognizing the partially folded state of cognate glycoproteins and reglucosylating their deglucosylated N-glycans. Notably, UGGT1 is known to form heterodimers with Selenoprotein F (SelenoF), although the mechanism underlying the complex formation remains unclear. To date, there have been no reports of large-scale expression systems for recombinant human UGGT1, and the formation of the human UGGT1-SelenoF complex has not been demonstrated <em>in vitro</em>. To address this, we used an insect cell-based expression system for heterologous expression of human UGGT1 and SelenoF, achieving high purity and efficiency superior to that of <em>Escherichia coli</em> expression systems. Importantly, the two proteins, prepared independently, were observed to form complexes <em>in vitro</em>. The establishment of a system for large-scale production of human UGGT1 and UGGT1-SelenoF complexes paves the way for future studies investigating their structural characteristics and dynamic behavior.</div></div>","PeriodicalId":20757,"journal":{"name":"Protein expression and purification","volume":"231 ","pages":"Article 106719"},"PeriodicalIF":1.4,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-throughput optimization of peptide-linker for fusing function protein with GFP 功能蛋白与绿色荧光蛋白融合肽连接体的高通量优化

IF 1.4 4区生物学

Protein expression and purification Pub Date : 2025-04-14 DOI: 10.1016/j.pep.2025.106718

Gaili Cao , Zhong Li , Zhaoguan Wang , Youhui Yang , Jiawei Li , Hao Qi

{"title":"High-throughput optimization of peptide-linker for fusing function protein with GFP","authors":"Gaili Cao , Zhong Li , Zhaoguan Wang , Youhui Yang , Jiawei Li , Hao Qi","doi":"10.1016/j.pep.2025.106718","DOIUrl":"10.1016/j.pep.2025.106718","url":null,"abstract":"<div><div>Fusion proteins are pivotal in bioengineering, with applications in purification, delivery, and imaging. However, the development of specialized peptide linkers tailored for target fusion proteins remains an unmet challenge. In this study, we demonstrate the optimization of fusing a functional protein with green fluorescent protein (GFP) through the screening of peptide linker sequences. Using seamless cloning methodology, a nanobody protein was fused to the N-terminus of GFP via a randomized 18-amino acid peptide linker library. Initial screening of fusion protein clones was conducted on solid plates to identify those expressing robust GFP fluorescence. A total of 153 clones with unique linker sequences were identified using Sanger sequencing. A wide range of normalized fluorescence signals was observed, revealing significant variability in linker performance. Among the screened linkers, one exhibited high fluorescence activity, outperforming commonly used flexible and rigid linkers. This finding underscores the necessity of optimize linker sequences for specific fusion proteins. Furthermore, the results demonstrated that the screened linker is compatible with diverse N-terminal proteins while maintaining GFP functionality. Additionally, to investigate the effect of linker on the function of target protein, we determined the reverse transcription efficiency of the murine leukemia virus reverse transcriptase (MLV-RT) in the fusion proteins by a two-step RT-qPCR method. In conclusion, this study presents an efficient optimization of peptide linkers, offering a novel methodology for the engineering and application of specialized linkers for fusion proteins.</div></div>","PeriodicalId":20757,"journal":{"name":"Protein expression and purification","volume":"231 ","pages":"Article 106718"},"PeriodicalIF":1.4,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening and functional studies of Norrin-related nanobodies 诺林相关纳米体的筛选与功能研究

IF 1.4 4区生物学

Protein expression and purification Pub Date : 2025-04-09 DOI: 10.1016/j.pep.2025.106717

Yiwen Xu , Yiang Wang , Zhongyun Lan , Yaxin Tuo , Siyu Zhou , Shuaiying Zhao , Yunfeng Liu , Yingying Kong , Huarui Qiao , Jianfeng Xu , Yuanyuan Dai , Yong Geng

{"title":"Screening and functional studies of Norrin-related nanobodies","authors":"Yiwen Xu , Yiang Wang , Zhongyun Lan , Yaxin Tuo , Siyu Zhou , Shuaiying Zhao , Yunfeng Liu , Yingying Kong , Huarui Qiao , Jianfeng Xu , Yuanyuan Dai , Yong Geng","doi":"10.1016/j.pep.2025.106717","DOIUrl":"10.1016/j.pep.2025.106717","url":null,"abstract":"<div><div>Norrin is a crucial regulator of the Wnt/β-catenin signaling pathway, playing a key role in retinal vascular development, blood-brain barrier maintenance, and neuroprotection. In this study, a Norrin fusion protein (Norrin-Fc) was successfully expressed and purified using an insect cell expression system. Camels were immunized with purified Norrin-Fc to generate nanobodies, which consist solely of the heavy chain variable domains of heavy chain antibodies (VHH) with a molecular weight of approximately 15 kDa. Two nanobody strains, Nb 1C4 and Nb 2B10, were identified for their specific binding to Norrin-Fc, and their binding affinities were further characterized. Flow cytometry analysis confirmed that Nb 1C4 and Nb 2B10 specifically bound to the Norrin-FZD4 fusion protein. Luciferase reporter assay results demonstrated that both nanobodies effectively disrupted LGR4-induced Wnt/β-catenin signaling upon Norrin stimulation. This study represents the first successful development of nanobodies targeting Norrin, providing a strong foundation for the advancement of Norrin-related diagnostic tools and antibody therapeutics.</div></div>","PeriodicalId":20757,"journal":{"name":"Protein expression and purification","volume":"231 ","pages":"Article 106717"},"PeriodicalIF":1.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The tumor suppressor LZTR1: Its expression, purification and characterization 肿瘤抑制因子 LZTR1：其表达、纯化和表征

IF 1.4 4区生物学

Protein expression and purification Pub Date : 2025-04-09 DOI: 10.1016/j.pep.2025.106716

Yifang Sun, Yuxuan Jiang, Meng Zhang, Lei Sun

{"title":"The tumor suppressor LZTR1: Its expression, purification and characterization","authors":"Yifang Sun, Yuxuan Jiang, Meng Zhang, Lei Sun","doi":"10.1016/j.pep.2025.106716","DOIUrl":"10.1016/j.pep.2025.106716","url":null,"abstract":"<div><div>Leucine-zipper transcription regulator 1 (LZTR1) serves as a tumor suppressor gene that is highly mutated and has been implicated in the pathogenesis of diverse cancers and developmental disorders. The LZTR1 protein is a member of the BTB-Kelch superfamily and functions as an adaptor that enables the recognition and recruitment of RAS proteins, which are then targeted for ubiquitination by the Cullin3-RING ligase E3 (CRL3) complex. Understanding the assembly mechanisms, substrate recognition patterns, and pathological processes associated with RAS ubiquitination mediated by LZTR1-CUL3 is critical. In this study, we report the expression, purification, and characterization of the LZTR1 to investigate its molecular mechanisms. Our findings demonstrate that the BacMam expression system significantly enhances LZTR1 production and simultaneously improves its stability and solubility. Furthermore, the existence of CRL3 contributes to stabilizing and homogenizing LZTR1 through facilitating the formation of the complex. Additionally, we identified MRAS as a substrate that binds tightly to LZTR1, in contrast to RIT1 and HRAS. Successfully expressing and purifying the full-length CRL3<sup>LZTR1</sup>-MRAS complex provide a foundation for future structural and functional investigations and offer a potential approach for exploring other BTB proteins with similar characteristics.</div></div>","PeriodicalId":20757,"journal":{"name":"Protein expression and purification","volume":"231 ","pages":"Article 106716"},"PeriodicalIF":1.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “High-affinity nanobodies targeting IL-12B for the detection of fluorescence resonance energy transfer” [Protein Express. Purificat. 229 (2025) 106681] 针对IL-12B检测荧光共振能量转移的高亲和力纳米体的勘误表[Protein Express]。净化。229 (2025)106681]

IF 1.4 4区生物学

Protein expression and purification Pub Date : 2025-04-09 DOI: 10.1016/j.pep.2025.106714

Jing Hu , Wenxuan Feng , Jianchuan Wen , Siyu Zhou , Zengchao Sun , Shuaiying Zhao , Shaojue Guo , Hui Wang , Yong Geng

引用次数: 0

Bioinformatics analysis and polyclonal antibody preparation of Mesocricetus auratus DHX30 auratus中囊绦虫DHX30生物信息学分析及多克隆抗体制备。

IF 1.4 4区生物学

Protein expression and purification Pub Date : 2025-04-06 DOI: 10.1016/j.pep.2025.106715

Keyi Wang , Jing Huang , Mingzhi Li , Tianxiang Han , Ju Yao , Decheng Kong , Bao Liu , Xiaojin Xia , Yunli Guo , Sha Li , Lingbao Kong

{"title":"Bioinformatics analysis and polyclonal antibody preparation of Mesocricetus auratus DHX30","authors":"Keyi Wang , Jing Huang , Mingzhi Li , Tianxiang Han , Ju Yao , Decheng Kong , Bao Liu , Xiaojin Xia , Yunli Guo , Sha Li , Lingbao Kong","doi":"10.1016/j.pep.2025.106715","DOIUrl":"10.1016/j.pep.2025.106715","url":null,"abstract":"<div><div>DHX30, as members of the DExD/H family, plays a crucial regulatory role in RNA metabolism and antiviral innate immune response. To facilitate comprehensive investigation of DHX30's multifunctional involvement in viral life cycles, a highly specific anti-DHX30 antibody is needed. In this paper, we used bioinformatic analyses combined with the Immune Epitope Database (IEDB) to identify immunodominant B-cell epitopes in the <em>Mesocricetus auratus</em> DHX30 protein. A hydrophilic peptide sequence demonstrating strong antigenic potential was selected for recombinant antigen production. The corresponding DHX30 truncated gene fragment was cloned into the pET-28a vector to generate the pET-28a-DHX30-N expression construct. Following transformation into <em>E. coli</em> BL21(DE3) competent cells, recombinant protein expression was induced and subsequently purified via Ni-NTA affinity chromatography. Six-week-old female KM mice were immunized intraperitoneally with the purified antigen. The antibody titer was determined by indirect ELISA, demonstrating high serum titers. Specificity validation through immunoblotting and immunocytochemical analyses confirmed the antibody's exceptional target recognition capability. These antibodies are expected to develop detection methods for further research.</div></div>","PeriodicalId":20757,"journal":{"name":"Protein expression and purification","volume":"231 ","pages":"Article 106715"},"PeriodicalIF":1.4,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143812165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0