Progress in clinical and biological research最新文献_第4页

Unique structural and biological features of Helicobacter pylori lipopolysaccharides. 幽门螺杆菌脂多糖的独特结构和生物学特性。

Progress in clinical and biological research Pub Date : 1998-01-01

A P Moran, G O Aspinall

引用次数: 0

Reconciling the concepts of endotoxin sensitization and tolerance. 调和内毒素致敏和耐受性的概念。

Progress in clinical and biological research Pub Date : 1998-01-01

M A Freudenberg, R Salômao, A Sing, I Mitov, C Galanos

引用次数: 0

The molecular basis for therapeutic concepts utilizing CD14. 利用CD14治疗概念的分子基础。

Progress in clinical and biological research Pub Date : 1998-01-01

F Stelter, M Bernheiden, R Menzel, S Witt, R S Jack, U Grunwald, X Fan, C Schütt

{"title":"The molecular basis for therapeutic concepts utilizing CD14.","authors":"F Stelter, M Bernheiden, R Menzel, S Witt, R S Jack, U Grunwald, X Fan, C Schütt","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The CD14 molecule is a key receptor on myeloid lineage cells involved in the recognition of lipopolysaccharide (LPS) and Gram-negative bacteria. The application of its soluble form, sCD14, has been shown to protect mice from lethality in LPS-induced shock. Therefore the protein or its derivatives may be considered as a possible therapeutic alternative for the treatment of patients suffering from Gram-negative septic shock. In this study we performed an alanine scan of amino acids 1 to 152 of human CD14. Twenty-three substitution mutants were generated and stably transfected into CHO-cells. In each mutant five amino acids were substituted by alanine. We analyzed (a) whether mutant proteins expressed on the surface of transfectants were recognized by a panel of anti-CD14 monoclonal antibodies (mAb's), (b) the ability of mCD14-mutants to bind LPS and E. coli in a serum- or LBP-dependent manner, and (c) the capacity of soluble mutants to mediate the LPS-induced IL 6 release of U 373 astrocytoma cells. Twenty-one CD14-mutants were expressed on the surface of transfectants and 18 were present as soluble forms in the culture supernatants. We demonstrated that only CD14(39-41,43-44)A completely lacked the ability to bind LPS and E. coli. In addition, a combined mutant CD14(9-13/57,59,61-63)A had very limited capacity to interact with LPS indicating that the LPS-binding site of human CD14 is a conformational epitope. Analysis of LPS-induced activation of CD14-negative U 373 cells revealed that the regions 9-13 and 91-101 are most important for sCD14-mediated signalling.</p>","PeriodicalId":20686,"journal":{"name":"Progress in clinical and biological research","volume":"397 ","pages":"301-13"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20497118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Roles of CD14 in LPS signaling and scavenging: analysis of CD14-transgenic and non-transgenic mice and rats in response to LPS. CD14在LPS信号传导和清除中的作用:CD14转基因和非转基因小鼠和大鼠对LPS的反应分析。

Progress in clinical and biological research Pub Date : 1998-01-01

S Yamamoto, Y Tamura, Y Higuchi, N Takai, S Akizuki, M Kataoka, K Matsuura

引用次数: 0

Endotoxin and sepsis: molecular mechanisms of pathogenesis, host resistance, and therapy. Proceedings of the 4th Conference of the International Endotoxin Society. Nagoya, Japan, October 23-27, 1996. 内毒素与败血症:分子机制、发病机制、宿主耐药性和治疗。第四届国际内毒素学会会议论文集。1996年10月23日至27日，日本名古屋。

Progress in clinical and biological research Pub Date : 1998-01-01

引用次数: 0

Prevention of endotoxin shock through targeting leukocyte adhesion molecules. 靶向白细胞粘附分子预防内毒素休克。

Progress in clinical and biological research Pub Date : 1998-01-01

H Higashi, N Mukaida, A Harada, S Watanabe, N Ikeda, Y Suzuki, K Matsushima

引用次数: 0