Proceedings of the Society for Experimental Biology and Medicine最新文献_第2页

The effect of fasting on the secretion of lipoproteins and two forms of apo B by perfused rat liver. 禁食对灌注大鼠肝脏脂蛋白及两种载脂蛋白B分泌的影响。

Proceedings of the Society for Experimental Biology and Medicine Pub Date : 1982-06-01 DOI: 10.3181/00379727-170-41415

J B Marsh, C E Sparks

{"title":"The effect of fasting on the secretion of lipoproteins and two forms of apo B by perfused rat liver.","authors":"J B Marsh, C E Sparks","doi":"10.3181/00379727-170-41415","DOIUrl":"https://doi.org/10.3181/00379727-170-41415","url":null,"abstract":"Abstract Rat livers from animals fed ad lib or fasted 24 hr were perfused by the single pass technique with 3H-amino acids and label incorporation into lipoprotein apoproteins was measured. The lipoproteins were separated into very low density lipoproteins (d < 1.006), low-density lipoproteins (1.006 < d < 1.06), and high-density lipoproteins (1.06 < d < 1.21) by ultracentrifugation and the apoproteins of each density fraction were analyzed by gel filtration column chromatography in the presence of sodium dodecyl sulfate. The apoprotein fractions included apo Bh (higher-molecular-weight apo B), apo B1 (lower-molecular-weight apo B), apo E, and apo C. Fasting decreased total hepatic apoprotein secretion from 75 to 53 μg·g−1·hr−1 and label incorporation into the apoproteins of very low density lipoproteins was reduced by 48% with the greatest reduction in both apo B fractions. There was a redistribution of apo Bh label into the low-density lipoproteins with fasting resulting in secretion of lipoproteins enriched in apo Bh. The high-density lipoprotein fraction contained 27 and 41% of the apo B1 label isolated from liver perfusates of fasted and fed animals, respectively. The nascent high-density lipoprotein was enriched in labeled apo B1 compared to low-density lipoproteins indicating that apo B in this density range represents a distinct metabolic fraction. With fasting the apo Bh and apo B1 apoproteins behave independently in terms of the distribution of label incorporation into nascent hepatic lipoproteins.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"178-81"},"PeriodicalIF":0.0,"publicationDate":"1982-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-170-41415","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35262358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

Agglutination of Naegleria fowleri by human serum. 人血清对福氏奈格氏杆菌的凝集作用。

Proceedings of the Society for Experimental Biology and Medicine Pub Date : 1982-06-01 DOI: 10.3181/00379727-170-41420

M F Reilly, M K Bradley, S G Bradley

引用次数: 5

The luteotrophic effects of estradiol and prolactin in the absence of LH in the hysterectomized, pseudopregnant rat. 在黄体生成素缺失的情况下，雌二醇和催乳素对子宫切除假妊娠大鼠黄体生成素的影响。

Proceedings of the Society for Experimental Biology and Medicine Pub Date : 1982-06-01 DOI: 10.3181/00379727-170-41419

D R Garris

{"title":"The luteotrophic effects of estradiol and prolactin in the absence of LH in the hysterectomized, pseudopregnant rat.","authors":"D R Garris","doi":"10.3181/00379727-170-41419","DOIUrl":"https://doi.org/10.3181/00379727-170-41419","url":null,"abstract":"Abstract The ability of estradiol and prolactin to substitute for LH as luteotrophins was investigated in Day 5 (Day 1 = ovulation)-hysterectomized pseudopregnant (PSP) rats. All animals received either a sc injection of oil, 1 μg estradiol (E-1), or 150 μg prolactin (PRL) on Day 9 in combination with either a normal horse serum (NHS) or LH antiserum (LHAS) injection. The ability of oil, E-1, or PRL to maintain luteal function was assessed by monitoring serum progesterone levels through Day 12 and their direct effects on the ovary monitored by measuring luteal and extra-luteal ovarian tissue concentrations of estradiol and progesterone on Day 12. NHS treatment in combination with either oil, E-1, or PRL maintained elevated serum progesterone levels through Day 12 of PSP. LHAS/oil-treated rats underwent luteolysis while E-1 and PRL effectively maintained luteal function in LHAS-treated animals. PRL raised luteal and extraluteal ovarian estradiol concentrations compared to oil/NHS-treated controls and LHAS/oil-treated rats. E-1 induced an intraluteal rise in estradiol levels in LHAS-treated rats. While luteal progesterone concentrations fell following oil/LHAS treatment, E-1 and PRL effectively counteracted this LHAS-induced fall. The results of these studies indicate that (i) E-1 and PRL can effectively replace LH as luteotrophins during PSP in the rat and (ii) that PRL and E-1 effectively maintain luteal levels of estradiol and progesterone following LH deprivation. It is suggested that E-1 and PRL may exert their luteotrophic actions by either an E-1-induced increase in PRL which, in turn, acts on the luteal cells to increase intraluteal estradiol concentrations, or by a direct effect of E on the corpus luteum to bypass the requirement for LH.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"203-8"},"PeriodicalIF":0.0,"publicationDate":"1982-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-170-41419","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35262359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Assay for thrombopoietin: a new, more sensitive method based on measurement of the small acetylcholinesterase-positive cell. 血小板生成素测定:一种新的、更灵敏的基于小乙酰胆碱酯酶阳性细胞测定的方法。

Proceedings of the Society for Experimental Biology and Medicine Pub Date : 1982-06-01 DOI: 10.3181/00379727-170-41421

G D Kalmaz, T P McDonald

{"title":"Assay for thrombopoietin: a new, more sensitive method based on measurement of the small acetylcholinesterase-positive cell.","authors":"G D Kalmaz, T P McDonald","doi":"10.3181/00379727-170-41421","DOIUrl":"https://doi.org/10.3181/00379727-170-41421","url":null,"abstract":"Abstract A sensitive assay for a thrombocytopoiesis-stimulating factor (TSF or thrombopoietin) that utilizes measurement of the small acetylcholinesterase-positive (SAChE+) cell in the marrow of mice is described. The results of this assay were compared with a previously published procedure that measures 35S incorporation into platelets of immuno-thrombocythemic mice. In the rebound-thrombocytotic mouse assay, TSF from kidney cell culture medium caused a dose-related increase in the amount of 35S incorporation into platelets; the minimum detectable dose of TSF was 15 mg of protein/mouse. For the SAChE+ cell assay, mice received single intraperitoneal injections of TSF or platelet-specific antiserum. Other mice were injected with plasma from thrombocytopenic donor mice. For controls, groups of mice were given saline or normal mouse plasma. Bone marrow from the mice (killed at 10 hr after injection) was taken for smears and stained for acetylcholinesterase. The results show that both TSF and rabbit anti-mouse platelet serum (RAMPS) caused a highly significant dose-dependent increase in the percentage of SAChE+ cells and that plasma from thrombocytopenic mice stimulated an increase (P < 0.0005) in the percentage of SAChE+ cells. The minimum detectable dose of TSF in the SAChE+ cell assay was 1.875 mg protein/mouse. Therefore, SAChE+ cells in the marrow of mice can detect smaller doses of TSF than the rebound-thrombocytotic mouse assay.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"213-9"},"PeriodicalIF":0.0,"publicationDate":"1982-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-170-41421","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35262361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

LACK OF THYMIC EFFECT ON WOUND HEALING. 对伤口愈合缺乏胸腺作用。

Proceedings of the Society for Experimental Biology and Medicine Pub Date : 1965-05-01 DOI: 10.3181/00379727-119-30099

E R FISHER, B FISHER

引用次数: 5

FACTORS INFLUENCING UPTAKE OF TRIIODOTHYRONINE-I-131 BY BULL SPERMATOZOA. 影响公牛精子摄取三碘甲状腺原氨酸- i -131的因素。

Proceedings of the Society for Experimental Biology and Medicine Pub Date : 1965-05-01 DOI: 10.3181/00379727-119-30087

J J SULLIVAN, A C MENGE

{"title":"FACTORS INFLUENCING UPTAKE OF TRIIODOTHYRONINE-I-131 BY BULL SPERMATOZOA.","authors":"J J SULLIVAN, A C MENGE","doi":"10.3181/00379727-119-30087","DOIUrl":"https://doi.org/10.3181/00379727-119-30087","url":null,"abstract":"Summary The uptake of triiodothyronine-I-131 by washed bull spermatozoa was found to have a positive association with sperm cell concentration and period of incubation with the labeled T3. Spermatozoa suspended in a simple salt solution (2.9% sodium citrate) had a higher uptake of T3 131 than spermatozoa diluted in a more complex ionic medium (N-J solution). The T3-I131 uptake by the sperm cells suspended in the 2 diluents increased at different rates with an increase in cell concentration. Within the range studied, addition of glucose to the 2 diluents had no effect on uptake of T3-I131 by spermatozoa. Sperm cell viability greatly influenced their uptake of T3-I131. Cells killed by continuous incubation at 38 °C or by treatment with acetic acid or sodium hydroxide had similar isotope uptake values which were significantly less than that of live cells. Spermatozoa killed by cold shock treatment, however, had the lowest uptake of T3-I131. Whereas, formaldehyde treatment of spermatozoa resulted in uptake values significantly greater than that of live cells. A positive association between uptake of T3-I131 and motility occurred with spermatozoa stored up to 18 days in different diluents and temperatures.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"20-4"},"PeriodicalIF":0.0,"publicationDate":"1965-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-119-30087","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40796822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

EVIDENCE FOR THE PRESENCE OF STEM CELLS IN THE TAIL OF THE MOUSE. 干细胞存在于老鼠尾巴的证据。

Proceedings of the Society for Experimental Biology and Medicine Pub Date : 1965-05-01 DOI: 10.3181/00379727-119-30142

C V ROBINSON, S L COMMERFORD, J L BATEMAN

引用次数: 23

SOME CYTOLOGIC CHANGES OF RAT LIVER IN EXPERIMENTAL PORPHYRIA. 实验性卟啉症大鼠肝脏细胞学变化。

Proceedings of the Society for Experimental Biology and Medicine Pub Date : 1965-05-01 DOI: 10.3181/00379727-119-30143

F I LOTTSFELDT, R F LABBE

引用次数: 6

BUPHTHALMIA IN THE RABBIT: A TEST FOR EARLY DIAGNOSIS. 兔眼热:一种早期诊断试验。

Proceedings of the Society for Experimental Biology and Medicine Pub Date : 1965-05-01 DOI: 10.3181/00379727-119-30144

R R FOX, E J BABINO

引用次数: 8

LIPID COMPONENTS OF BILE FROM CHOLINE DEFICIENT RATS. 胆碱缺乏大鼠胆汁的脂质成分。

Proceedings of the Society for Experimental Biology and Medicine Pub Date : 1965-05-01 DOI: 10.3181/00379727-119-30147

I C WELLS, J M BUCKLEY

引用次数: 3