Proceedings of the National Academy of Sciences of Belarus, Biological Series最新文献

{"title":"Fauna of aquatic invertebrates of the spring ecosystems in the Minsk region","authors":"V. М. Baitchorov, М. D. Moroz, Y. G. Hihiniak, I. Y. Giginyak, A. A. Kulikova, J. V. Korzun","doi":"10.29235/1029-8940-2024-69-1-25-35","DOIUrl":"https://doi.org/10.29235/1029-8940-2024-69-1-25-35","url":null,"abstract":"New data on aquatic invertebrates of the spring ecosystems of the Minsk region are presented. 104 lowest defined taxa (LDT) species and forms of representatives of macrozoobenthos and pleistone complexes belonging to 4 phyla of aquatic invertebrates were found: Platyhelminthes – 2, Mollusca – 20, Annelida – 7, Arthropoda – 75 species and forms. 75 taxonomic elements have been identified before the species. The average number of identified aquatic invertebrate species in the studied springs of the Minsk region was 12‒13 species, and the number of 205 specimens. The sources are inhabited by protected species of animals listed in the Red Book of Belarus and the Red Lists of a number of Western European countries. It was concluded that the fauna of aquatic invertebrate animals is rich and represented by a number of species rare in Belarus and Europe.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139853476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of moisture deficiency on the apple stress-associated protein expression level .","authors":"Р. V. Kuzmitskaya, К. S. Karaleva, О. Y. Urbanovich","doi":"10.29235/1029-8940-2024-69-1-36-46","DOIUrl":"https://doi.org/10.29235/1029-8940-2024-69-1-36-46","url":null,"abstract":"In plants, stress-associated proteins (SAP) are involved in response to adverse biotic and abiotic factors. In order to study the drought effect on changes in the SAP expression level in apple trees and their involvement in stress response, an expression level of fourteen genes belonging to this family was analyzed using qPCR. Their participation in a stress response to drought in the genome of the MM-106 apple tree rootstock was shown. At that, the genes demonstrated a tendency to increase their expression by the fourth hour of drought exposure followed by its decrease by the twenty-fourth hour.A comparison of the data obtained during the described experiment with the data obtained when modeling drought under milder conditions showed that under more severe drought conditions, an expression of a greater number of gene encoding stress-associated proteins increases, and a more pronounced response occurs. Evaluation of the effect of an identity degree of nucleotide sequences of gene encoding stress-associated proteins on the similarity of their expression profiles under drought exposure did not reveal any direct relationship between the primary structure of genes and the nature of their expression.In the structure of stress-associated proteins encoded by the genes most strongly response to drought in Malus, Solanum lycopersicum, Gossypium hirsutum, and Cucumis sativus, the A20-AN1 type domain occurs more frequently than other types of zinc finger domains. Analysis of the similarity of stress-associated protein sequences and the expression levels of genes encoding them under drought conditions in different plant species (apple, cotton, tomato, and cucumber) suggests that in each species, the evolution of adaptation mechanisms, including SAP encoding genes, occurred independently and, most likely, after separation of those species from their common ancestor. The results obtained will make it possible to clarify the role of individual genes, encoding SAP, in the formation of an apple stress response to the drought effect.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139851316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological and biochemical features of implementation of the adaptive potential of clonally micropropagated potato plants using immunostimulants","authors":"N. V. Baliuk, N. Laman, J. Kalatskaja","doi":"10.29235/1029-8940-2024-69-1-15-24","DOIUrl":"https://doi.org/10.29235/1029-8940-2024-69-1-15-24","url":null,"abstract":"The effect of epibrassinolide in combination with salicylic acid and methyl jasmonate on the formation of complex resistance of clonally micropropagated potato plants of the Briz variety to the potato virus Y and to moisture lack, their physiological state, the functioning of the pro-/antioxidant system, as well as on the productivity and quality of the obtained mini-tubers was studied. It was revealed that antiviral resistance under combined stress conditions is formed only in the presence of epibrassinolide that triggers a series of protective mechanisms through accumulation of hydrogen peroxide, phenolic compounds and an increase in peroxidase activity, while maintaining productivity and improving the quality of the products obtained. The use of a three-component mixture is accompanied by the largest mass and a number of potato minitubers, while its protective effect against a viral infection is not manifested.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139851865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity of apple trees in the old orchard of the Central Botanical Garden of the National Academy оf Sciences of Belarus","authors":"A. A. Famina, A. N. Zainchkovskaya, Р. V. Kuzmitskaya, О. Y. Urbanovich, Р. А. Pashkevich, L. S. Sidor, В. Y. Anoshenko, L. V. Goncharova","doi":"10.29235/1029-8940-2024-69-1-57-67","DOIUrl":"https://doi.org/10.29235/1029-8940-2024-69-1-57-67","url":null,"abstract":"Based on the sequence of the Golden Delicious apple genome, whose nucleotide sequence is located in the GenBank database, the design of molecular markers of SSR type that limit the area of tetra- and hexanucleotide repeats was carried out in silico. The most informative of them were selected, which showed clear peaks on capillary electrophoresis and made it possible to reveal a high level of polymorphism in certain apple genome regions. The developed SSR markers can be effectively used for the genetic diversity assession and DNA identification of apple varieties, establishment of the varietal correspondence. These markers were used to analyze the genetic diversity of apple trees in the old orchard of the Central Botanical Garden of the National Academy of Sciences of Belarus (CBG) where trees were planted from 1932 to 1940. It is shown that the trees growing in the garden are genetically close to old local varieties, as well as to individual foreign ones. It is established that among the trees of the CBG old orchard there are such varieties as Antonovka obyknovennaya, Minskoe, Papirovka. Wealthy, Melba, Bogatyr’. The obtained results will be used to save the genetic resources of the fruit crop valuable for the country, which is an apple tree, as well as to develop a design project of a new fruit plants collection-exposition when reconstructing the area of old plantings.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139792937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamics of change in the number of amphibian larvae in pond conditions and factors determining it in the central part of Belarus","authors":"J. V. Korzun, A. A. Kulikova","doi":"10.29235/1029-8940-2024-69-1-47-56","DOIUrl":"https://doi.org/10.29235/1029-8940-2024-69-1-47-56","url":null,"abstract":"The aim of the work is to study the influence of factors of abiotic and biotic origin on abundance, growth rate and development of amphibian larvae in natural conditions.The scientific novelty of the work lies in studying the degree of interaction of amphibian larvae with each other and with invertebrate predators, their interaction in natural conditions. The main part of the work contains a literature review, the work methodology and the result description.In conclusion, it was noted that under natural conditions, the magnitude of impact of predators on the number of amphibian larvae is much lower than it has been previously thought; they are not able to provide a significant function of regulating the number of tadpoles. Abiotic environmental factors, being within the optimum limits, do not affect the mortality of larvae, and food in natural water bodies is not a factor limiting abundance. The most intensive death of larvae occurs in the early larval development stage. Despite a low overall density of larvae in a natural water body, most amphibian larvae are periodically exposed to metabolites and are affected by the “group effect”. This is expressed in the inhibition of growth and development of individuals, the death or decrease in the decline in the viability of the most sensitive individuals, and also increases the likelihood of their death from other factors.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139793445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial activity of Thuja occidentalis essential oils and extracts","authors":"N. A. Kovalenko, G. N. Supichenkо, Т. I. Ahramovich, Y. A. Niachai, V. N. Leontiev","doi":"10.29235/1029-8940-2024-69-1-79-88","DOIUrl":"https://doi.org/10.29235/1029-8940-2024-69-1-79-88","url":null,"abstract":"The steam distillation method was used to obtain samples of essential oil of cultivars with a columnar and spherical shape of the crown Thuja occidentalis introduced in the Republic of Belarus. More than 26 components have been identified and determined in the essential oil of Thuja occidentalis by gas-liquid chromatography. The dominant components are α-thujone (∼55–60 %), β-thujone (∼10–12 %), fenchone (∼8–16 %), sabinene (∼1–5 %), camphor (∼1–4 %), bornylacetate (∼1–3 %). Distributions of terpenoid enantiomers in the essential oil of Thuja occidentalis cultivars are shown. The antimicrobial activity of Thuja occidentalis essential oils was detected in relation to test cultures of gram-positive and gram-negative bacteria. Bacterial cultures were more sensitive to the inhibitory effect of essential oil samples from plants with a spherical crown shape. The influence of the optical activity of camphor on the essential oil antimicrobial properties is shown. It has been established that alcohol extracts of Thuja occidentalis are inferior to essential oils in terms of antimicrobial activity. The effect of the crown shape and the color of the studied cultivar needles on their antioxidant properties is shown.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139851280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variability of plastic features of size groups of the american catfish Ameiurus nebulosus (Lesueur, 1819) – alien species in the water bodies of Belarus","authors":"Yu. I. Okhremenko, H. Gajduchenko","doi":"10.29235/1029-8940-2023-68-3-257-264","DOIUrl":"https://doi.org/10.29235/1029-8940-2023-68-3-257-264","url":null,"abstract":"A scheme for measuring the plastic features of the brown bullhead, including 28 morphometric characteristics, has been proposed. Based on the cluster analysis of morphometric characteristics, the size groups of species of a model water body were determined (Karpin village pond, Maloritsky district, Brest region).The change in the exterior parameters of the brown bullhead during its ontogenesis has been shown for the first time. It is proposed to divide brown bullhead individuals into 3 size groups for analysis: A – young individuals not involved in reproduction (body length without a caudal fin up to 135.0 mm); B – mature individuals that have started breeding (sizes from 136.0 to 174.0 mm); C – adults, actively breeding individuals (sizes from 175.0 to 187.0 mm).","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84353734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of a temperature shock on the synthesis efficiency of surfactants by Rhodococcus pyridinivorans 5Ap bacteria","authors":"H. Bukliarevich, M. Titok","doi":"10.29235/1029-8940-2023-68-3-224-233","DOIUrl":"https://doi.org/10.29235/1029-8940-2023-68-3-224-233","url":null,"abstract":"It was found that the synthesis of surfactants by R. pyridinivorans 5Ap bacteria can be increased by exposing them to a short temperature shock (55 °C for 20 minutes) after 24 hour cultivation in a minimal medium containing molasses (3 %) and hexadecane (2 %) (9 % increase in the emulsification index). Gene activation encoding global regulators of cell metabolism, including those performing a protective function under stress, was recorded in this cultivation mode. In particular, the mRNA amount determining the synthesis of the alternative transcription factor SigH increased 90.8 times and containing in the promoters its binding sites fmdB cochaperone – 59.3 times, hsp22.5 chaperone – 81.1 times, and the trxB gene encoding thioredoxin reductase – 73.1 times. In addition, it was shown that the transcriptional activation of groEL1, groEL2 and dnaJ genes determining the synthesis of heat shock proteins was 2.2, 2.6 and 4.4 times, respectively. The obtained results suggest that an increase in the alternative factor sigma H synthesis, which activates protective cellular metabolism, as well as structural, heat shock proteins under short temperature stress, leads to an increased production of surfactants, which can be used to optimize the synthesis of these secondary metabolites for biotechnological use.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80334163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of disulfide bonds in the formation of the spatial structure of the human epidermal growth factor","authors":"A. A. Akunevich, V. V. Khrustalev, T. Khrustaleva, L. Kordyukova, A. Arutyunyan","doi":"10.29235/1029-8940-2023-68-3-183-196","DOIUrl":"https://doi.org/10.29235/1029-8940-2023-68-3-183-196","url":null,"abstract":"The epidermal growth factor (EGF) is a peptide of the EGF-like growth factor family with a common conserved EGF-like domain formed by three intramolecular disulfide bonds. This article describes changes in the spatial structure of EGF and its mutant form with the D46G substitution in its C-terminal fragment observed upon disulfide bonds reduction in the corresponding synthetic peptides in a 0.01 M phosphate buffer (pH = 7.4). The structure was analyzed using circular dichroism spectroscopy, spectrofluorimetry, native polyacrylamide gel electrophoresis, and centrifugal ultrafiltration. It was shown that disulfide bonds reduction changes the geometry of the EGF-like domain towards an increase in the content of the beta-structure, while these peptides remain in dimeric form. According to the molecular modeling results, this can lead to the elongation of the main beta-hairpin of the EGF-like domain, to the elongation of the intermolecular beta-structure, or to the formation of a new beta-structure between the N- and C-terminal fragments of each molecule, which will change the intermolecular interface in dimeric form. Disulfide bonds reduction prevents EGF dimer dissociation to monomers. Under physiological conditions, this can lead to the inability of EGF to form binding sites for EGFR (epidermal growth factor receptor) and to cause its activation.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84295922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological changes of mitochondria under the diabetes condition and pharmacological approaches to eliminate them","authors":"U. Gayibov, H. Ruziboev, M. Y. Eraliev, M. Pozilov, M. Asrarov, N. Abdulladjanova, Ch. Xiandan, A. Sysa","doi":"10.29235/1029-8940-2023-68-3-197-205","DOIUrl":"https://doi.org/10.29235/1029-8940-2023-68-3-197-205","url":null,"abstract":"The state of lipid peroxidation (LPO), respiration and oxidative phosphorylation, mitochondrial permeability transition pore (mPTP), and the antiradical activity of rat liver mitochondria in the streptozotocin (STZ)-induced diabetes condition were studied with the consideration of the ways of correcting detected membrane damages using gossitan isolated from the cotton plant Gossypium hirsutum L. It was shown that the rate of respiration of liver mitochondria in states V3 and V4 increases during STZ-induced diabetes, which significantly reduces the respiratory control (RC) and ADP/O coefficients in comparison with the control. The findings suggest that the uncoupling of respiration and oxidative phosphorylation takes place during STZ-induced diabetes. It was shown that in the STZ-induced diabetes condition, the rate of swelling of rat liver mitochondria is higher than that of the healthy ones; this means that mPTP of rat liver mitochondria is in the open state. Gossitan recovers mPTP to the normal condition, thereby removing the STZ effect on mitochondria. Gossitan (a personal dose is 10 mg/kg of body weight, during 8 days) eliminates the detected functional disorders of rat liver mitochondria, probably due to its antioxidant properties.","PeriodicalId":20656,"journal":{"name":"Proceedings of the National Academy of Sciences of Belarus, Biological Series","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78069299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}