PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY最新文献_第5页

Nanostructured minerals developed to be used as fertilizers: biosafety evaluation 纳米结构矿物用作肥料:生物安全性评价

PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY Pub Date : 2022-10-07 DOI: 10.21285/2227-2925-2022-12-3-438-446

I. Degtyareva, E. Babynin, E. Prishchepenko

{"title":"Nanostructured minerals developed to be used as fertilizers: biosafety evaluation","authors":"I. Degtyareva, E. Babynin, E. Prishchepenko","doi":"10.21285/2227-2925-2022-12-3-438-446","DOIUrl":"https://doi.org/10.21285/2227-2925-2022-12-3-438-446","url":null,"abstract":"Natural zeolites are effectively used as fertilizers, substrates, and pesticide carriers, as well as sorbents in the remediation of contaminated soils. Since nanostructured minerals exhibit unique physicochemical properties, they must be tested for toxicity and genotoxicity prior to their use in practice. The mutagenic and antimutagenic properties of a nanostructured water-zeolite suspension were first tested using two bacterial test systems: Ames test and SOS-lux test. According to the obtained data, the nanostructured water-zeolite suspension exhibits no mutagenic activity within the analyzed concentration range (0.75–400 µg/mL). In order to assess the antimutagenic activity of the nanostructured water-zeolite suspension, different types of mutagens were selected: mitomycin C, ethyl methanesulfonate, 2,4-dinitrophenylhydrazine, as well as DNA-damaging agents (ofloxacin and hydrogen peroxide). A significant antimutagenic effect of the nanostructured water-zeolite suspension at 200 μg/mL was shown against mitomycin C in the SOS-lux test (50.0% inhibition of mutagenic activity) and 2,4-dinitrophenylhydrazine in the Ames test (62.0% inhibition). For the other mutagens, a weak antimutagenic effect was observed (17.0% for ethyl methanesulfonate), while no antimutagenic effect was reported for ofloxacin and hydrogen peroxide. These differences can be attributed to the negative charge in zeolites, meaning that they can capture only positive (or neutral) molecules. Therefore, the antimutagenic effect of the nanostructured water-zeolite suspension depends on the charge of the mutagen molecule. According to the obtained results, the nanostructured water-zeolite suspension can be considered environmentally friendly, which allows it to be used for agro-industrial purposes as a fertilizer in the production of crops.","PeriodicalId":20601,"journal":{"name":"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85079900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of endophytic and epiphytic nitrogen-fixing bacteria on the content of negative allelopathic compounds in root exudates of pea (Pisum sativum L.) seedlings 内生固氮菌和附生固氮菌对豌豆幼苗根系分泌物中负化感物质含量的影响

PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY Pub Date : 2022-10-06 DOI: 10.21285/2227-2925-2022-12-3-394-405

L. Makarova, I. Petrova, N. Sokolova, S. S. Makarov, V. Pionkevich

{"title":"Influence of endophytic and epiphytic nitrogen-fixing bacteria on the content of negative allelopathic compounds in root exudates of pea (Pisum sativum L.) seedlings","authors":"L. Makarova, I. Petrova, N. Sokolova, S. S. Makarov, V. Pionkevich","doi":"10.21285/2227-2925-2022-12-3-394-405","DOIUrl":"https://doi.org/10.21285/2227-2925-2022-12-3-394-405","url":null,"abstract":"Substances that have a harmful effect on living organisms include N-phenyl-2-naphthalamine and phthalates, which are synthesized and widely used in the chemical industry. At the same time, N-phenyl-2-naphthylamine was found in the aerial parts and in the roots of some plant species, phthalates were found in many plant species and in bacteria. The aim of this research was to study the protective (antimicrobial) reaction of pea (Pisum sativum L.) seedlings of the Torsdag variety to the inoculation with bacteria Rhizobium leguminosarum bv. viceae (endosymbiont) and Azotobacter chroococcum (ectosymbiont) introduced into the aqueous medium of root growth were studied. Changes in the content of negative allelopathic compounds (pisatin, N-phenyl-2-naphthylamine, phthalates) in root exudates were the reaction indicators. After the inoculation, the seedlings grew for 24 h in the BINDER KBW-240 chamber at 21 °C, with lighting of 81 μM.m-2 . sec-1 and a 16/8 h day/night photoperiod. In ethyl acetate extracts from the aqueous medium where the seedling roots were immersed, the content of the compounds was determined by HPLC, while changes in the composition and ratio of phthalates were determined by GC-MS. Data indicating the different ability of both bacterial species to degrade N-phenyl-2-naphthylamine to phthalates and the dependence of this process activity in the bacteria studied on its concentration in the medium were presented. N-phenyl-2-naphthylamine differently but negatively affected the viability and growth of the bacteria used in the experiments. A different effect of rhizobia and azotobacter on the content of the above named compounds and on the ratio of types of phthalates in root exudates was elicited.","PeriodicalId":20601,"journal":{"name":"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81348186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prospects for chemical and biotechnological processing of miscanthus 芒草化学与生物技术加工展望

PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY Pub Date : 2022-10-06 DOI: 10.21285/2227-2925-2022-12-3-383-393

N. Shavyrkina, Yu. A. Gismatulina, V. Budaeva

{"title":"Prospects for chemical and biotechnological processing of miscanthus","authors":"N. Shavyrkina, Yu. A. Gismatulina, V. Budaeva","doi":"10.21285/2227-2925-2022-12-3-383-393","DOIUrl":"https://doi.org/10.21285/2227-2925-2022-12-3-383-393","url":null,"abstract":"The processing of plant biomass into demanded and economically viable products is currently a recognized global trend. Among alternative energy directions, biomass conversion is the most predictable and sustainable carbon resource that can replace fossil fuels. Already today, plant biomass provides almost 25% of the world’s energy supply. This review provides information on the most promising areas of chemical and biotechnological processing of the biomass of such an energy plant as miscanthus. The choice of miscanthus is due to its high yield (up to 40 t/ha of sown area) and high energy yield (140–560 GJ/ha) compared to other plant materials. In addition, miscanthus is able to grow on marginal lands and does not require special agronomic measures, while in the process of its cultivation, the soil is enriched with organic substances and it is cleaned from pollutants. The review reflects the directions of processing of native biomass and pretreated biomass. Miscanthus biomass, in addition to processing into energy resources, can be fractionated and transformed into many high-value products - cellulose, cellulose nitrates, ethylene, hydroxymethylfurfural, furfural, phenols, ethylene glycol, cooking solutions after nitric acid pretreatment of miscanthus biomass can act as lignohumic fertilizers. In addition, on the basis of miscanthus cellulose hydrolysates, it is possible to obtain benign nutrient media for biotechnological transformation into bacterial nanocellulose, for the accumulation and isolation of various microbial enzymes.","PeriodicalId":20601,"journal":{"name":"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79346868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Application of scanning electron microscopy and IR spectroscopy for a timely evaluation of the morphology and chemical composition of bacterial films during batch cultivation 应用扫描电子显微镜和红外光谱技术及时评价批培养过程中细菌膜的形态和化学成分

PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY Pub Date : 2022-10-06 DOI: 10.21285/2227-2925-2022-12-3-406-416

D. Faizullin, A. Kobelev, S. V. Klement’ev, A. Sirotkin, A. Rogov, V. Salnikov, O. Makshakova, Y. Zuev

{"title":"Application of scanning electron microscopy and IR spectroscopy for a timely evaluation of the morphology and chemical composition of bacterial films during batch cultivation","authors":"D. Faizullin, A. Kobelev, S. V. Klement’ev, A. Sirotkin, A. Rogov, V. Salnikov, O. Makshakova, Y. Zuev","doi":"10.21285/2227-2925-2022-12-3-406-416","DOIUrl":"https://doi.org/10.21285/2227-2925-2022-12-3-406-416","url":null,"abstract":"Bacteria most commonly exist in nature in the form of bacterial biofilms, i.e. associations of cells attached to a substrate surrounded by a polymer matrix. Research into biofilm formation and functioning is fundamental to the management of microbial associations in ecology, biotechnology and medicine. This task requires the development of analytical approaches capable of providing timely information throughout the life cycle of microbial communities at the same time as maintaining their intact structure. In this paper, we apply scanning electron microscopy and IR spectroscopy as rapid methods for analysing microbial biofilms. To this end, the growth kinetics of a Bacillus subtilis culture cultivated on a solid substrate for 24 h was comparatively studied by the methods of classical microbiology and biochemistry, electron scanning microscopy and Fourier-transform IR spectroscopy. The biofilm morphology was found to vary from a uniform settlement of planktonic cells over the substrate surface at the initial stage of growth (6 h) followed by the accumulation of the extracellular matrix and the formation of microcolonies at the exponential and stationary stage (12–18 h) and a gradual depletion of the matrix at the stage of cell death (24 h). The results of IR spectroscopy were established to agree well with those of biochemical studies, thereby demonstrating the potential of the method for a timely evaluation of the accumulation of proteins, polysaccharides and nucleic acids and for obtaining information about their structural state in the studied biofilm. It is concluded that scanning electron microscopy and Fourier-transform IR spectroscopy can be used for obtaining complementary information about the morphology and chemical composition of microbial biofilms during their cultivation.","PeriodicalId":20601,"journal":{"name":"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83517542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study into the hydrolysis of polyhexamethylene guanidine hydrochloride 聚六亚甲基胍盐酸盐的水解研究

PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY Pub Date : 2022-10-05 DOI: 10.21285/2227-2925-2022-12-3-356-362

O. Ochirov, S. Stel’makh, M. Grigor’eva, V. Okladnikova

{"title":"Study into the hydrolysis of polyhexamethylene guanidine hydrochloride","authors":"O. Ochirov, S. Stel’makh, M. Grigor’eva, V. Okladnikova","doi":"10.21285/2227-2925-2022-12-3-356-362","DOIUrl":"https://doi.org/10.21285/2227-2925-2022-12-3-356-362","url":null,"abstract":"The development of new preparations for managing skin lesions is a task requiring a complex research approach. Thus, one promising direction consists in the creation of new bases for wound-healing drugs for external application. Chemical compounds that can be used as such bases include polymeric hydrogels, representing spatially cross-linked macromolecules that swell in a solvent. This property provides an opportunity to load hydrogels with drugs of both synthetic and herbal origin. A search for a gel-forming polymer acting not only as a drug carrier, but also as a healing agent presents a relevant research task. In a previous work, the authors obtained polyhexamethylene guanidine hydrochloride hydrogels by crosslinking terminal amino groups with formaldehyde. The conducted studies of the wound-healing capacity of the obtained hydrogels and compositions on their basis confirmed their comparability with such widely-used agents, as levomecol, bepanthene, etc. In addition, the obtained compositions were found to exhibit their own activity. Therefore, hydrogels based on polyhexamethylene guanidine hydrochloride can be used as a promising platform for drug design. In this work, the destruction products released during hydrolysis of the hydrogel under study were investigated. IR and UV spectroscopy methods were applied to evaluate the concentration of hydrogel destruction products over time. A mechanism of hydrogel destruction yielding the initial polyhexamethylene guanidine hydrochloride and formaldehyde in a gem-diol form is proposed.","PeriodicalId":20601,"journal":{"name":"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY","volume":"111 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78312389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantum chemical simulation of hydrogen adsorption in pores: A study by DFT, SAPT0 and IGM methods 孔隙中氢吸附的量子化学模拟:基于DFT、SAPT0和IGM方法的研究

PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY Pub Date : 2022-10-05 DOI: 10.21285/2227-2925-2022-12-3-363-372

I. K. Petrushenko

{"title":"Quantum chemical simulation of hydrogen adsorption in pores: A study by DFT, SAPT0 and IGM methods","authors":"I. K. Petrushenko","doi":"10.21285/2227-2925-2022-12-3-363-372","DOIUrl":"https://doi.org/10.21285/2227-2925-2022-12-3-363-372","url":null,"abstract":"Hydrogen as a versatile energy carrier continues to attract research attention in the field of applied chemistry. One of the fundamental issues on the way to hydrogen economy is the difficulty of hydrogen storage. Physical adsorption of hydrogen in pores is a feasible and effective method of hydrogen storage. Among existing hydrogen-adsorbing materials, carbon nanostructures possess a number of advantages due to their high adsorption capacity, significant strength and low weight. In this work, we use the modern methods of quantum chemistry (DFT, SAPT0 and IGM) to study the adsorption of molecular hydrogen in a series of simulated slit-like carbon micropores with a distance between the walls of d = 4–10 Å, including the introduction of an H2 molecule into a pore, filling pores with these molecules and investigating the interactions between H2 molecules inside the pores. It was found that, depending on the value of parameter d, adsorbed hydrogen molecules form one (d = 6, 7 Å) or two layers (d = 8, 9, 10 Å) inside the pore. At the same time, for pores with small d values, high potential barriers to the introduction of H2 into a pore were observed. The decomposition of the interaction energy into components showed dispersion interactions to make a major contribution to the energy of attraction (72–82%). Moreover, an increase in the number of H2 molecules adsorbed in the pore decreases the significance of dispersion interactions (up to 61%) and increases the contribution of electrostatic and induction interactions to intermolecular attraction. Gravimetric density (GD) values were determined for pores with d = 6, 7, 8, 9, 10 Å, comprising 1.98, 2.30, 2.93, 3.25 and 4.49 wt%, respectively. It is assumed that the revealed peculiarities of hydrogen adsorption in pores will contribute to the use of carbon porous structures as a medium for hydrogen storage.","PeriodicalId":20601,"journal":{"name":"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88798805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Application of anthocyanins as indicators for alkalimetric titration 花青素作为碱滴定指示剂的应用

PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY Pub Date : 2022-10-05 DOI: 10.21285/2227-2925-2022-12-3-373-382

S. M. Dryutskaya, I. V. Tolstenok, N. Yakusheva

{"title":"Application of anthocyanins as indicators for alkalimetric titration","authors":"S. M. Dryutskaya, I. V. Tolstenok, N. Yakusheva","doi":"10.21285/2227-2925-2022-12-3-373-382","DOIUrl":"https://doi.org/10.21285/2227-2925-2022-12-3-373-382","url":null,"abstract":"Along with synthetic substances, various naturally-occurring pigments can response to pH variations in solutions by changing their colour. Such substances include, for example, anthocyanins. In this study, the possibility of applying a set of anthocyanins obtained from berry extracts as a potential acid-base indicator. This indicator can be a good alternative to the widely-used phenolphthalein test. Aqueous and alcoholic extracts derived from the fruit of ashberry, sea-buckthorn, lingonberry, blueberry, currant, blackberry, cranberry, rose hip, cherry and hawthorn plants were studied. The presence of anthocyanins in the test samples was confirmed by chemical and physicochemical methods of analysis, including qualitative tests, ascending thin-layer chromatography and direct spectrophotometry. Quantification of anthocyanins was carried out by pH-differential spectrophotometry. Compared to alcoholic extracts, aqueous berry extracts were found to contain anthocyanins in far lesser amounts. In order to compare the pH indicator potential of phenolphthalein and the studied set of anthocyanins, the content of ascorbic acid in the test samples was assessed by direct alkalimetry. A control experiment was performed using a 5% ascorbic acid solution for injection. The pH value of aqueous and alcoholic solutions of all berry extracts was determined by an instrumental procedure. The pH of the extracts ranged from 2.95 to 5.04. The content of the total amount of anthocyanins in aqueous and alcoholic extracts of blackberries, cranberries, black currants, blueberries and lingonberries in terms of cyanidin-3-glucoside was determined both quantitatively and qualitatively. A good agreement was achieved between the results of alkalimetric titration performed using phenolphthalein and the set of anthocyanins extracted from blackcurrant berries with 95% ethanol.","PeriodicalId":20601,"journal":{"name":"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91050593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The “Doctor Robik 109” complex biopreparation as a bioagent for utilizing aquatic plant phytomass in biofuel cells “dr . Robik 109”复合生物修复作为生物制剂，用于在生物燃料电池中利用水生植物生物量

PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY Pub Date : 2022-04-01 DOI: 10.21285/2227-2925-2022-12-1-50-63

D. Stom, G. Zhdanova, N. Yudina, S. Alferov, A. Chesnokova, M. Tolstoy, A. Kupchinsky, M. Saksonov, S. Zakarchevskiy, T. Enkhdul, A. Franzetti, M. Rahimnejad

{"title":"The “Doctor Robik 109” complex biopreparation as a bioagent for utilizing aquatic plant phytomass in biofuel cells","authors":"D. Stom, G. Zhdanova, N. Yudina, S. Alferov, A. Chesnokova, M. Tolstoy, A. Kupchinsky, M. Saksonov, S. Zakarchevskiy, T. Enkhdul, A. Franzetti, M. Rahimnejad","doi":"10.21285/2227-2925-2022-12-1-50-63","DOIUrl":"https://doi.org/10.21285/2227-2925-2022-12-1-50-63","url":null,"abstract":"The paper demonstrates the possibility of using the “Doctor Robik 109” complex microbiological preparation (OOO VIPEKO, Russia), applied in cesspools and sewage caissons, for obtaining electric current in biofuel elements during the utilization of the Ulothrix sp., Spirogyra sp., Elodea canadensis aqueous plant phytomass. The kinetics of electrical parameters of the studied biofuel cells was characterized by a steady and prolonged growth – for at least 30 days. This may be associated with the slow transformation of the used substrate by microorganisms, which additionally ensures the long-term operation of biofuel cells on their basis. In Spirogira sp. biofuel cells, the biological preparation generated the voltage (broken circuit mode) and current strength (short circuit mode) equal up to 746mV and 1745 A, respectively. In Ulothrix sp. biofuel cells, these values comprised 360 mV and 1120 μA for a 25-day period, respectively. When using a E. canadensis substrate, the studied bioagent microorganisms generated the voltage and current of up to 643 mV and 568 μA during a 25-day period, respectively. An increase in the electrical parameters of biofuel cells, which were based on the studied biological preparation and the phytomass of higher aquatic plants and algae, was accompanied by an increase in the number of viable microorganism cells and a decrease in the plant biomass. Since phytomass growth is activated by the phytoremediation measures of additional wastewater treatment or during the eutrophication of water bodies, the proposed method for applying aquatic plants in biofuel cells represents a promising approach to utilizing excess phytomass.","PeriodicalId":20601,"journal":{"name":"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78764124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of the haematopoietic and immune effect of microalgae extracts 微藻提取物造血和免疫作用的研究

PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY Pub Date : 2022-04-01 DOI: 10.21285/2227-2925-2022-12-1-109-120

A. Lykov, I. Uvarov, R. Gevorgiz, S. Zheleznova, O. Poveshchenko

{"title":"Study of the haematopoietic and immune effect of microalgae extracts","authors":"A. Lykov, I. Uvarov, R. Gevorgiz, S. Zheleznova, O. Poveshchenko","doi":"10.21285/2227-2925-2022-12-1-109-120","DOIUrl":"https://doi.org/10.21285/2227-2925-2022-12-1-109-120","url":null,"abstract":"Microalgae, as a source of numerous biologically active substances, are promising candidates for the development of novel biologically active supplements. An important aspect determining the possibility of using microalgae involves the absence of adverse effects on the organs and systems of the human and animal body. At the development stage, special attention should be paid to the safety of microalgae for biomedical use in terms of the absence of significant negative effects on the haematopoiesis and the functional status of immune cells. In this regard, the present study investigates the effect of oil extracts obtained from five microalgae species on haematopoietic parameters, the number and functional activity of spleen and thymus cells, as well as on immunoglobulin levels in the blood serum of laboratory animals. According to the obtained results, Chlorella vulgaris (C. vulgaris) and Cylindrotheca closterium (C. closterium) extracts decrease the number of leukocytes in the peripheral blood. Coelastrella sp., C. closterium, and Porphyridium purpureum (P. purpureum) increase the proportion of neutrophils. P. purpureum was found to initiate an increase in the proportion of eosinophils. Arthrospira (Spirulina) platensis (A. platensis) and C. closterium produce a decrease in the proportion of monocytes. C. closterium and P. purpureum induce a reduction in the proportion of lymphocytes in the peripheral blood of laboratory mice. All microalgae extracts had no effect on erythropoiesis and haemoglobin. In addition, all microalgae extracts were found to reduce splenocyte proliferation, while their effect on thymocyte proliferation depended on the microalgae type. In the mice serum, Coelastrella sp. microalgae extract increases IgG levels, while P. purpureum and C. vulgaris decrease IgM and IgA levels, respectively.","PeriodicalId":20601,"journal":{"name":"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY","volume":"90 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85842707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis and characterization of 2,5-dibutylthio-2,3-dihydro-2-formyl-4H-pyran thiosemicarbazones and their copper complexes 2,5-二丁基硫-2,3-二氢-2-甲酰基- 4h -吡喃硫代氨基脲及其铜配合物的合成与表征

PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY Pub Date : 2022-04-01 DOI: 10.21285/2227-2925-2022-12-1-167-172

E. Verochkina, N. V. Vchislo, L. Larina, E. Titov

{"title":"Synthesis and characterization of 2,5-dibutylthio-2,3-dihydro-2-formyl-4H-pyran thiosemicarbazones and their copper complexes","authors":"E. Verochkina, N. V. Vchislo, L. Larina, E. Titov","doi":"10.21285/2227-2925-2022-12-1-167-172","DOIUrl":"https://doi.org/10.21285/2227-2925-2022-12-1-167-172","url":null,"abstract":"Previously unknown derivatives of thiosemicarbazide were obtained in high yields by the condensation reaction of 2,5-dibutylthio-2,3-dihydro-2-formyl-4H-pyran with 4-phenylthiosemicarbazide or thiosemicarbazide when boiling in EtOH. The reaction products are represented by colored oils. Cu(II) complexes based on 2,5-dibutylthio-2,3-dihydro-2-formyl-4H-pyran carbazones were synthesized by their interaction with copper (II) chloride. All these complexes are soluble in dimethylformamide (DMF) and dimethyl sulfoxide (DMSO) and, according to elemental analysis, have a 1:1 metal-ligand stoichiometry. The obtained compounds were studied using IR and 1H, 13C, 15N NMR spectroscopy, as well as elemental analysis. The obtained copper-containing complexes with thiosemicarbazones are of particular interest due to their pronounced biological activity. The thiosemicarbazone 2,5-dibutylthio-2,3-dihydro-2-formyl-4H-pyran compound was subjected to primary screening for acute toxicity. The obtained results showed that, when taken intragastricaly, the studied compound can be characterized as a substance with a low risk of acute toxicity. According to the DL50 parameter, the compound belongs to the V hazard class (DL50>2000 mg/kg).","PeriodicalId":20601,"journal":{"name":"PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86816146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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