Plant breeding and biotechnology最新文献

{"title":"Prospects for obtaining low-alkaloid and adaptive forms of narrow-leafed lupinе based on the genome and transcriptome resources of the species","authors":"M. Vishnyakova, E. Krylova","doi":"10.30901/2658-6266-2022-2-o1","DOIUrl":"https://doi.org/10.30901/2658-6266-2022-2-o1","url":null,"abstract":"The narrow-leafed lupinе (Lupinus angustifolius L.) is considered as a crop of untapped opportunities. The food and forage potential of the species is not fully exploited due to the presence of quinolizidine alkaloids (QA) in plants, which are secondary metabolites that make the seeds bitter and toxic to humans and animals. Varieties with a low content of QA (“sweet” varieties) created over the last 50-60 years turned out to be more susceptible to damage by sucking insects and insect-transmitted viruses than high alkaloid ones (“bitter” varieties). Based on the rapidly developing genomic, transcriptomic and metabolomic profiling of the species, some molecular determinants and features of alkaloid biosynthesis in narrow-leafed lupinе plants have been identified: alkaloids are formed in the vegetative organs of the plant and then transported to the seeds. This information substantiated the creation of “bitter-sweet” forms with a high content of alkaloids in the vegetative parts of the plant, which would make it possible to reduce the attack of pathogens, and a minimal content of alkaloids in the seeds. This review summarizes the existing prerequisites for obtaining such forms of narrow-leafed lupinе on the basis of the available scientific developments. Information on the creation of saturated genetic maps of the species, in which the iucundus (iuc) locus determining the overall low alkaloid content in seeds is integrated and is used in breeding programs. The use of the new generation sequencing allowed the identification of the RAP2-7 gene, encoding the transcription factor APETALA2/ETHYLENE RESPONSE FACTOR, which is coupled to the iuc locus and located in the area with the main QTLs that affect the composition of the QA. It is a likely candidate gene for regulating alkaloid content in narrow-leafed lupinе seeds. The initial stages of QA biosynthesis and its regulatory factors have been revealed. Two reference assemblies of the genome of narrow-leafed lupinе have been carried out. All these achievements constitute a valuable resource for the creation of forms of narrow-leafed lupinе with a high content of alkaloids in the vegetative mass and low in the seeds, which are absent in nature.","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79710521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity Analysis of Bangladeshi Coastal Rice Landraces (Oryza sativa) for Morpho-Physiological and Molecular Markers' Responses to Seedling Salinity Tolerance","authors":"Hafsa Sultana, U. Somaddar, S. Samanta, A. K. Chowdhury, G. Saha","doi":"10.9787/pbb.2022.10.2.115","DOIUrl":"https://doi.org/10.9787/pbb.2022.10.2.115","url":null,"abstract":"","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41965412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Seedling Screening Method for Low-Temperature Tolerance in Pumpkin (Cucurbita moschata) and Selection of the Tolerant Resources for Rootstock of Cucumber","authors":"Chae-Rin You, Hemasundar Alavilli, Kihwan Song","doi":"10.9787/pbb.2022.10.2.102","DOIUrl":"https://doi.org/10.9787/pbb.2022.10.2.102","url":null,"abstract":"","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46508351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contribution of IR20 and IR64 in Developing Three Bangladeshi Popular Rice Cultivars","authors":"M. Ahmed, A. Biswas, S. Afrin","doi":"10.9787/pbb.2022.10.2.81","DOIUrl":"https://doi.org/10.9787/pbb.2022.10.2.81","url":null,"abstract":"","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43187929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-Wide Association Study of Blast Resistant in Korean Rice (Oryza sativa L.) Breed Lines","authors":"Tae-Ho Ham, Ja-Hong Lee, Seong-Gyu Jang, Muhyun Kim, Hongjia Zhang, Na-Eun Kim, Soon-Wook Kwon, Joohyun Lee","doi":"10.9787/pbb.2022.10.2.139","DOIUrl":"https://doi.org/10.9787/pbb.2022.10.2.139","url":null,"abstract":"","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42933012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of Gene Expression Related to Salt Tolerance with Sorghum (Sorghum bicolor L. Moench) Mutants","authors":"J. Seo, J. Lyu, Jung Min Kim, N. Hung, Joon-Woo Ahn, C. Kim, B. Ha, Soon-Jae Kwon","doi":"10.9787/pbb.2022.10.2.128","DOIUrl":"https://doi.org/10.9787/pbb.2022.10.2.128","url":null,"abstract":"","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42230628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colorimetric and Chlorophyll Fluorescence Assays for Fast Detection and Selection of Transgenic Events of Cotton, Cowpea, Soybean and Common Bean Expressing the Atahas Gene","authors":"C. T. Citadin, M. P. Santos, E. R. Andrade, Thaís M. Cipriano, F. Aragão","doi":"10.9787/pbb.2022.10.2.94","DOIUrl":"https://doi.org/10.9787/pbb.2022.10.2.94","url":null,"abstract":"","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45292784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maintenance of potato varieties in in vitro and field collections of the Russian Potato Research Centre","authors":"E. Oves, N. Gaitova, O. Shishkina","doi":"10.30901/2658-6266-2022-1-o5","DOIUrl":"https://doi.org/10.30901/2658-6266-2022-1-o5","url":null,"abstract":"The main biological feature of potato varieties is vegetative reproduction. This mode of reproduction can be associated with problems due to the physiological ageing of the crop and the accumulation of specific pathogens causing reduced tuber yields. In order to avoid these problems, potato seed production widely uses modern biotechnological methods. The use of meristemic technologies allows preserving the identity of the biomaterial in the process of maintaining the potato collection in vitro, but even under these conditions there is a threat that modifications of individual economically valuable traits may get fixed. In potato varieties, such non-heritable deviations manifest themselves in the form of a shift in phenophases and the period of tubers ripening. The use of modern high-tech methods of varietal resources storage implemented on the basis of biotechnological approaches, makes it possible to maintain high quality of biomaterial. At the same time, mobility and practicality remain the main criteria for the effectiveness of different storage methods, depending on the extent to which they can be used in practice. In this review, the collection of varieties at Russian Potato Research Center is used as an example for considering the main stages of the formation and functioning of a modern Bank of Healthy Potato Varieties (BHPV), which supplies various regions of the Russian Federation with high-quality phytopathogen-free potato varieties.","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85652649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On the anniversary of Lyudmila Andreevna Bespalova, an outstanding breeder, Vice President of the Vavilov Society of Geneticists and Breeders (VOGiS), Academician of the Russian Academy of Sciences (RAS)","authors":"A. Nizhnikov, E. Khlestkina, I. Tikhonovich","doi":"10.30901/2658-6266-2022-1-o3","DOIUrl":"https://doi.org/10.30901/2658-6266-2022-1-o3","url":null,"abstract":"April 2, 2022 marks the anniversary of Lyudmila Andreevna Bespalova, an Academician of the Russian Academy of Sciences, one of the prominent wheat and triticale breeders, the author of many varieties introduced into agricultural practice both in our country and abroad, which are cultivated in areas encompassing millions of hectares, thus making a significant contribution to solving the global food security problem. Lyudmila A. Bespalova is a recognized classic in the field of wheat breeding and seed production, she has published over 300 scientific papers, and received about 120 copyright certificates and patents. Acad. Bespalova is a bright representative of domestic breeders, who maintains the traditions of one of the largest scientific schools and takes an active part in the life of the community of geneticists and breeders in this country by training young specialists and increasing the prestige of and demand for the profession of a breeder.","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74811752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryopreservation of raspberry cultivar accessions bred in Russia from the VIR in vitro collection","authors":"A. M. Kamnev, S. E. Dunaeva, N. N. Volkova, O. Lisitsyna, T. Gavrilenko","doi":"10.30901/2658-6266-2022-1-o2","DOIUrl":"https://doi.org/10.30901/2658-6266-2022-1-o2","url":null,"abstract":"Cryobanks use plant cryocollections for long-term preservation of crops which cannot be preserved in seed collections. These are vegetatively propagated crops, accessions of species which form either a small amount of seeds, or recalcitrant seeds. Shoot tips (apexes) of in vitro plants are used for cryopreservation for most berry crops, therefore maintenance of in vitro collections is very important. The VIR in vitro collection includes 150 accessions of Rubus L. species, 85 of them are raspberry cultivars, 59 of which were bred in Russia. These cultivars reflect a wide ecogeographic diversity. Among them, there are raspberry cultivars created at the end of the 19th – first half of the 20th centuries, including cultivars bred by I.V. Michurin and by the pioneer of northern horticulture V.V. Spirin. More than half of national raspberry varieties (33) are listed in the State Register for Selection Achievements Admitted for Usage. Raspberry cultivars from Russian breeding programs have a very limited representation in foreign genebanks. The first aim of the present work was cryopreservation of mostly folk and old Russian raspberry cultivars received by VIR from 1925 till 1950 and their transfer into the cryobank. The second aim of the work was to monitor post-cryogenic regeneration of raspberry cultivars transferred to the cryobank earlier. A modified protocol of the droplet vitrification method by “DV-biotech” was used for cryopreservation of shoot tips of in vitro plants of 10 raspberry cultivars (7 of which are folk and old Russian ones) from the VIR in vitro collection. Post-cryogenic regeneration was evaluated for 17 raspberry cultivars preserved in the cryobank from one to five years. Ten raspberry cultivars (900 apexes) with an average mean post-cryoregenic regeneration value of 38.2±3.0% determined in control tests, were placed in the cryobank for long-term storage. A statistically significant effect of the genotype on the viability of explants after cryopreservation was noted, while the post-cryogenic regeneration was genotype insensitive. Additionally, levels of post-cryogenic regeneration were evaluated for 17 raspberry cultivars (296 apexes) preserved in the cryobank from one to five years. Post-cryogenic regeneration within the 20-70% range was displayed by four raspberry cultivars preserved in the cryobank for one year, and for 8 cultivars conserved there from three to five years post-cryogenic regeneration was within the 10-50% range. According to the results of monitoring, regeneration displayed by 12 raspberry cultivars was within the 10-70% range, which can be considered as a reliable rate of apex preservation in liquid nitrogen vapors in the VIR cryobank. Monitoring of the post-cryogenic regeneration of the raspberry accessions preserved in the VIR cryobank and cryopreservation of new raspberry cultivars will be continued.","PeriodicalId":20582,"journal":{"name":"Plant breeding and biotechnology","volume":"78 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76116253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}