Problems Of Biological, Medical And Pharmaceutical Chemistry最新文献

{"title":"ANTIOXIDANT PROPERTIES OF THE DIHYDROQUERCETIN \u0000WITH ALPHA-TOCOPHEROL COMBINATIONS \u0000IN FREE RADICAL OXIDATION","authors":"","doi":"10.29296/25877313-2024-01-02","DOIUrl":"https://doi.org/10.29296/25877313-2024-01-02","url":null,"abstract":"Introduction. A significant factor in the manifestation of the compound antioxidant ability is a pattern of its interaction with other antioxidants, which affects the demonstrated final antioxidant effect. Flavonoids display different antioxidant capacities along with a wide range of biological activity, dihydroquercetin in particular. The role of the regenerating component in the cycles of mutual transformations in antioxidant systems is assumed for dihydroquercetin as it's a compound with a high profile of antiradical capacity. Here we focused on the nature of the antioxidant activity presentation in a composition consisting of dihydroquercetin and the essential antioxidant α-tocopherol. The combination was studied in a wide ratio range of the dihydroquercetin-α-tocopherol composition components from 1:1 to 1:20, based on the assumption that the concentration of the second component in living systems is excessive.\u0000Aim. The work aimed to determine the nature of the dihydroquercetin-α-tocopherol composition antioxidant activity in vitro, both in general and in terms of the individual component’s action.\u0000Material and methods. The antioxidant effects of the composition were studied within the framework of decolorization and kinetic methods based on the inhibition of model radical cations of 2,2'-azino-bis-(3-ethylbenzthiosoline-6-sulfonic acid) diammonium salt. To determine the nature of the individual components' action, high-resolution mass spectrometry was used, based on the analysis of the spectra of which kinetic curves of the consumption of the components of the composition were constructed.\u0000Results. A study of the interaction of dihydroquercetin with α-tocopherol by the decolorization method showed that a slight subadditive effect was observed for all ratios of the components of the compositions. The kinetic method revealed a two-stage nature of the antioxidant activity manifestation of the composition, with the first stage of the induction period being attributed to the α–tocopherol action, and the second to dihydroquercetin. In support of the hypothesis expressed, it was shown by high-resolution mass spectrometry that a decrease in the concentration of dihydroquercetin in the composition begins only after almost complete α-tocopherol consumption.\u0000Conclusions. α-Tocopherol prevents the oxidation of dihydroquercetin by radical cations ABTS•+ when acting together in the composition, and regeneration of one antioxidant by another does not occur.","PeriodicalId":20508,"journal":{"name":"Problems Of Biological, Medical And Pharmaceutical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140470580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MEDICINAL PLANTS WITH RESTORATIVE AND TONIC EFFECT \u0000IN THE REPUBLIC OF BURYATIA (REVIEW)","authors":"P. G. Mizina, I. Lupanova, V. V. Karabaeva, E.V. Ferubko, O.A. Altaeva, O.M. Cybikova, O.Yu. Davydova","doi":"10.29296/25877313-2024-01-01","DOIUrl":"https://doi.org/10.29296/25877313-2024-01-01","url":null,"abstract":"Relevance. In many countries plants have long been used in folk medicine as a source of medicines, as they are well tolerated, gradual development of therapeutic effect and a mild effect on the body. Due to these features such medicines are being safely used in the treatment and prevention of exacerbations of chronic diseases: of cardiovascular system, respiratory system, digestive system, pathologies of the urinary tract, etc., and also as a rehabilitation therapy after past diseases.\u0000The purpose of the study is to systematize and generalize the data of world literature concerning the general health-improving and tonic properties study of plants growing in the Republic of Buryatia.\u0000Material and methods. In this work used publication materials from the PubMed and е-library databases, search.rsl. The keyword search: restorative, tonic effect, herbal medicines, medicinal plants of Buryatia, biologically active substances, Astragalus membranaceus, Saposhnikovia divaricatа, Scutellaria baicalensis, Sedum roseum, Crataegus sangunea. The survey comprises the data of foreign and national articles, published on the topic during last 20 years.\u0000Conclusions. All the plants listed in this review are used for thousands of years in the Tibetan medicine. Based on the analysis of the presented literature data, their range of medicinal use is much wider, then the applications in modern clinical practice. The information reported in this review may be the basis for the development of new herbal medicines, including officinal mixture, with specified pharmacological properties.","PeriodicalId":20508,"journal":{"name":"Problems Of Biological, Medical And Pharmaceutical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140472735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DEVELOPMENT OF APPROACHES TO THE ANALYSIS \u0000OF ELEMENTAL IMPURITIES IN TITANIUM DIOXIDE \u0000(BARIUM AND LEAD DEFINITION)","authors":"I.V. Paskar, S.P. Senchenko, O.A. Kapiturova, E.V. Borkovskaya, N.G. Paskar","doi":"10.29296/25877313-2024-01-04","DOIUrl":"https://doi.org/10.29296/25877313-2024-01-04","url":null,"abstract":"Relevance. Titanium dioxide (TiO2) is widely used in the pharmaceutical industry as a drug excipient (DE). Therefore, control of its content of ele-mental impurities (EI) plays an important role in ensuring the stability of quality and safety of drugs. The lack of harmonized pharmacopoeial require-ments for the content of EIs in TiO2, as well as the need for specialized validated methods of their analysis determine the actual task in the field of quality control of this substance.\u0000Research objective. This work is devoted to the development and validation of a method for the determination of barium and lead in TiO2 sub-stance.\u0000Material and Methods. The inductively coupled plasma atomic emission spectrometry (ICP-AES) method was used to determine the barium and lead content in the TiO2 substance. Acid extraction was used in the sample preparation process. All used reagents and materials were in accordance with pharmacopoeial requirements. A sample of TiO2 substance, manufactured by Venator Germany GmbH, Germany, was used as an object of study. Validation of the method was carried out in accordance with the requirements of GF RF for the following characteristics: specificity, linearity, LOQ, correctness, repeatability, intra-laboratory precision and range of the technique.\u0000Results. The method of barium and lead determination in TiO2 substance using ICP-AES demonstrates high accuracy and sensitivity. The results ob-tained during validation meet the acceptance criteria, which confirms the applicability of the technique for control of barium and lead content in TiO2 substance.\u0000Conclusions. A method for the determination of barium and lead impurities in TiO2 substance using the ICP-AES method and acid extraction during sample preparation has been developed. This approach enables the determination of barium and lead at the specification level (20 ppm and 5 ppm, re-spectively). The validation results meet the acceptance criteria for all characteristics investigated, confirming the ability to quantify barium and lead in the range of 50% to 150% of the specification level.","PeriodicalId":20508,"journal":{"name":"Problems Of Biological, Medical And Pharmaceutical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140475124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COMPONENT COMPOSITION OF ESSENTIAL OIL \u0000THYMUS PULCHELLUS S. A. MEY., \u0000INTRODUCED UNDER DAGESTAN CONDITIONS","authors":"F. Vagabova, A. M. Aliev","doi":"10.29296/25877313-2024-01-07","DOIUrl":"https://doi.org/10.29296/25877313-2024-01-07","url":null,"abstract":"The purpose of the study is to study the component composition of the essential oil in the aerial part of the endemic Krasnodar specimen Thymus pulchellus S. A. Mey., transplanted to the Tsudakhar experimental base of the Mountain Botanical Garden of the Dagestan Federal Research Center of the Russian Academy of Sciences (CEB, 1000 m above sea level, Dagestan, Russia ) to search for plants with a high yield of essential oil with a maximum content of valuable biologically active components and expand the growing area of this type of thyme. \u0000Material and methods. The aerial part of the Thymus pulchellus introduced at the CEB was collected in the flowering phase. The essential oil was obtained by hydrodistillation (Clevenger method) for 2 hours from dry raw materials prepared by conventional methods. Component qualitative and quantitative analysis of the isolated essential oil samples was carried out by chromatography-mass spectrometry on a Shimadzu GCMSQP2010plus on a Supelco SLB TM-5 ms column (30 m × 0.25 mm × 0.25 μm) in split mode. \u0000Results. The EF yield was 0.26% in terms of air-dry raw materials. Using chromatography-mass spectrometry, 56 components were isolated from EF samples, of which 97.67% of compounds were identified. \u0000Conclusions. For the first time, data are presented on the study of the composition of the essential oil from the aerial part of the introduced Dagestan sample of Thymus pulcellus. The resulting essential oil consists mainly of the sesquiterpene fraction (84.12%). Compared to other species, the essential oil of the studied pretty thyme showed a completely different set of main components, among which the main ones are limonene, γ-cadinene, α-muurolol, epicubenol, which allows us to assert the isolation of this species. The results obtained allow us to characterize the essential oil of Thymus pulchellus as a source of valuable components, and the plant itself as a promising medicinal raw material.","PeriodicalId":20508,"journal":{"name":"Problems Of Biological, Medical And Pharmaceutical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140478106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OBTAINING A WORKING STANDARD SAMPLE OF CHALCONE \u0000BUTEINE AND ITS QUANTITATIVE DETERMINATION \u0000IN PLANT RAW MATERIALS","authors":"E. O. Kulichenko, E. T. Oganesyan, S. V. Pechinskii, A. G. Kuregyan, M. Larsky, A. P. Pleten, A. A. Prokopov, T. Tatarenko-Kozmina","doi":"10.29296/25877313-2024-01-03","DOIUrl":"https://doi.org/10.29296/25877313-2024-01-03","url":null,"abstract":"Introduction. Butein ‒ 2',4',3,4-tetrahydroxychalcone is found in a number of plants that have a pronounced antimicrobial and antifungal effect: Butea monosperma Lam., hairy string ‒ Bidens pilosa L., tripartite string ‒ Bidens tripartita L., hydrophytum ‒ Hydnophytum formicarum Jack., early thyme ‒ Thymus praecox. Butein is not the dominant representative of the flavonoid class in plants, but it can serve as a marker compound for the analysis of raw materials containing this chalcone. \u0000The purpose of the study is to synthesize and analyze a working standard sample of butein, as well as to develop a method for its quantitative determination in Cosmos bipinnate raw materials using solid-phase extraction. \u0000Material and methods. Butein synthesis was carried out using the Claisen‒Schmidt reaction, the yield was 44,4%. Identification of the obtained butein was carried out using UV, IR and Mass spectroscopy. Quantitative determination of butein in the raw materials of Cosmos doubly persita was carried out by HPLC in combination with solid-phase extraction. \u0000Results. The results obtained confirm that the test sample is butein (2',4',3,4-tetrahydroxychalcone). To determine butein in plant raw materials, a standard sample of this compound should be available, and therefore the substance of this chalcone was synthesized and on its basis, according to the state pharmacopoeia, a working standard sample was obtained (butein content in it is 99,62%). Identification of butein by HPLC was carried out by the retention time, which in the chromatogram of the test solution is 160,70 min, in the chromatogram of the standard solution – 160,07 min  2%. The butein content in the flowers of the cosmos bipinnate we studied in terms of dry raw materials is 0,043%  2%. \u0000Conclusions. The synthesis of chalcone ‒ butein was carried out by alkaline condensation of resacetophenone with protocatechuic aldehyde. The yield of butein is 5,95  0,0195 g (44,4%). A working standard sample was obtained based on the butein substance, the authenticity and purity of which was confirmed by melting point, as well as using HPLC, mass spectroscopy, UV and IR spectroscopy. The resulting standard working sample was further used to develop a method for the quantitative determination of butein in Cosmos bipinnate flowers using solid-phase extraction. The butein content in terms of dry raw materials is 0,043%3%.","PeriodicalId":20508,"journal":{"name":"Problems Of Biological, Medical And Pharmaceutical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140471813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BIOLOGICAL FEATURES OF HEDYSARUM ALPINUM L. CELL CULTUREWHEN GROWN IN VITRO","authors":"P. S. Savin, N. S. Tsybulko, S. B. Myasnikova","doi":"10.29296/25877313-2024-01-08","DOIUrl":"https://doi.org/10.29296/25877313-2024-01-08","url":null,"abstract":"The purpose of the research conducted was to obtain a cell culture of Hedysarum alpicum, as a producer of xanthone mangiferin, by achieving the tasks of obtaining a cell culture of H. alpium growing in deep-cultivation conditions and studying its physiological characteristics. \u0000Material and methods. The object of the study was a cell culture of H. alpine obtained from the cotyledon of the germination, strain H. alpinum (C) 2016 of the collection of FSBSI «All-Russian Scientific Research Institute of Medicinal and Aromatic Plants» and the native plant grown on the pharmacopeial site of the Botanical Garden of FSBSI «All-Russian Scientific Research Institute of Medicinal and Aromatic Plants», harvest of 2013. \u0000Results. It has been established that growth regulators kinetin and 2.4D stimulate growth processes of H. alpium (C) cell culture 2016 in deep cultivation conditions. The duration of growth is 20–23 days. The maximum increase corresponds to 16 g/l on the 20th day of growth. The maximum consumption of oxygen by the cell culture is 30·104 mol/l·h on the 16th day of growth, when the cells are in the regular growth phase. \u0000Conclusion. As a result of the work carried out, a stable cell strain of H. alpium was obtained, growing on the surface of the agarized nutrient medium, which has the potential for the synthesis of mangiferin and work has been carried out on studying its physiological characteristics.","PeriodicalId":20508,"journal":{"name":"Problems Of Biological, Medical And Pharmaceutical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140471797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"APPLICATION OF SELECTIVE HYPROMELLOSE SORBENT \u0000FOR THE BENZOCAINE QUANTITATION \u0000IN PHARMACEUTICAL FORMULATIONS","authors":"М.N. Kachalkin, A. V. Voronin","doi":"10.29296/25877313-2024-01-05","DOIUrl":"https://doi.org/10.29296/25877313-2024-01-05","url":null,"abstract":"Introduction. In quality control of pharmaceutical formulations, there are methodological approaches based on the preliminary separation of sample components and approaches without preliminary separation. The use of solid-phase extraction (SPE) allows to separate analytes during sample prepa-ration, with the separation selectivity determining the analysis selectivity as a whole. Currently, sorbents that provide selectivity in interaction with the analyte are of interest. \u0000Aim. Evaluation of the metrological characteristics of benzocaine quantitation by UV-spectrophotometry in certain pharmaceutical formulations using hypromellose sorbent for solid-phase extraction.\u0000Material and methods. The sorbent using a method developed by us was obtained. Benzocaine (FS.2.1.0634, Russia State Pharmacopoeia XV ed.) to form active binding sites in sorbent structure was applied. The sorbent structure is polycyanoacrylate matrix with hypromellose fragments, the sur-face area is 255.50 m2/g, pore volume is 0.1433 cm3, pore diameter is 5.32 nm. The sorption capacity of hypromellose sorbent for benzocaine was 12.2±0.8 μg/g. The SPE technique is proposed. SPE includes the stages: conditioning, sample addition and step-by-step elution with purified water and hydrochloric acid solution 0.1 mol/L. The spectrophotometer SF-56 was used to measure the absorbance at an analytical wavelength of 286 nm. To evaluate the selectivity (specificity) of sample preparation during chromatographic (SPE) separation of pharmaceutical formulations, absorption spectra of eluates in the wavelength range of 200400 nm were scanned. The benzocaine identification in the eluate was carried out based on absorption peaks at 220 and 286 nm. The benzocaine calibration curve in the range of 120 μg/ml. To determination the metrological characteristics of benzocaine quan-titation method, 11 parallel determinations of samples of each pharmaceutical formulation were made. \u0000Results. When realizing the benzocaine quantitation technique for measuring absorbance, it is advisable to use only the first portions of eluate (a solu-tion of hydrochloric acid) in a volume of 510 ml were obtained. The relative error of benzocaine average concentration in the pharmaceutical formula-tions ranged from 1.28 to 1.34% for technique that included the SPE stage, and from 1.86 to 2.02% for technique that did not include it. A comparison of the modifications of the spectrophotometric quantitation of benzocaine in pharmaceutical formulation using hypromellose sorbent and technique without the SPE did not reveal a statistically significant difference in reproducibility. For the modification technique without SPE, an increase of benzo-caine concentration and in determination relative error by an average of 48.0% were observed.\u0000Conclusion. The possibility of using a selective hypromellose sorbent for sample preparation for benzocaine quantitation determination in pharmaceu-tical formulations has been showed. The use of a sorb","PeriodicalId":20508,"journal":{"name":"Problems Of Biological, Medical And Pharmaceutical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140485524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the efficiency of use of a biologically active supplement based on the extract of galega officinalis in the complex therapy of type 2 diabetes mellitus","authors":"V. Burkova, V. Sergun, A. A. Ivanov, Y. Samoylova, O. Oleinik","doi":"10.29296/25877313-2022-09-06","DOIUrl":"https://doi.org/10.29296/25877313-2022-09-06","url":null,"abstract":"","PeriodicalId":20508,"journal":{"name":"Problems Of Biological, Medical And Pharmaceutical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74418610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an identification method Vaccinium uliginosum leaves by thin-layer chromatography","authors":"A. A. Shamilov, V. N. Bubenchikova, E. Garsiya, Kh.A. Ibaeva, M. Larsky","doi":"10.29296/25877313-2022-11-02","DOIUrl":"https://doi.org/10.29296/25877313-2022-11-02","url":null,"abstract":"","PeriodicalId":20508,"journal":{"name":"Problems Of Biological, Medical And Pharmaceutical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74774833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling and dynamics of endogenous and exogenous oxidative stress in vitro","authors":"Y. Abalenikhina, S. K. Pravkin, A. Shchulkin, E. Rokunov, D.S. Nemtinov, E.P. Vasilyeva, E. Yakusheva","doi":"10.29296/25877313-2022-12-02","DOIUrl":"https://doi.org/10.29296/25877313-2022-12-02","url":null,"abstract":"","PeriodicalId":20508,"journal":{"name":"Problems Of Biological, Medical And Pharmaceutical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81819293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}