发布求助
文献互助 智能选刊 最新文献

Preparative biochemistry最新文献

筛选
英文 中文
Concentration of solutes by freezing and centrifugation. 通过冷冻和离心浓缩溶质。
Preparative biochemistry Pub Date : 1993-02-01 DOI: 10.1080/10826069308544539
{"title":"Concentration of solutes by freezing and centrifugation.","authors":"","doi":"10.1080/10826069308544539","DOIUrl":"https://doi.org/10.1080/10826069308544539","url":null,"abstract":"The virus extraction rotor may be usefully applied for concentrating solutes such as low molecular weight substances, proteins, virus soluble antigens and picorna viruses by centrifuging frozen dilute solutions at moderately high rotor velocities in the virus extraction rotor which had been fitted with the filters and precooled to 0�C. To accomplish this a circular stainless steel container with inner diameter equal to the inner diameter of the insert minus 5 mm was made. The reason for the smaller diameter was to allow for the thickness of the set of filters which lined the inner surface of the rotor insert. A rod attached to a flat circular metal slab closed a hole in the base of the cylinder by means of an 0 ring in the metal slab. To conduct a concentration experiment the surface of the 0 ring was covered lightly with silicon grease and inserted in the groove on the bottom of the metal slab.","PeriodicalId":20391,"journal":{"name":"Preparative biochemistry","volume":"23 1-2","pages":"79-80"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10826069308544539","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19352980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Purification of potato leaf roll virus. 马铃薯叶卷病毒的纯化。
Preparative biochemistry Pub Date : 1993-02-01 DOI: 10.1080/10826069308544555
A Polson
{"title":"Purification of potato leaf roll virus.","authors":"A Polson","doi":"10.1080/10826069308544555","DOIUrl":"https://doi.org/10.1080/10826069308544555","url":null,"abstract":"ABSTRACT Potato leaf roll virus was purified by a combination of Takanami and Kubo's method and the use of the extraction and thin layer rotors and finally by centrifugation in tubes filled with beads.","PeriodicalId":20391,"journal":{"name":"Preparative biochemistry","volume":"23 1-2","pages":"267-71"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10826069308544555","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19354426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Preparative immunoabsorption electrophoresis. 1978. 制备性免疫吸收电泳。1978.
Preparative biochemistry Pub Date : 1993-02-01 DOI: 10.1080/10826069308544537
A Polson, M B von Wechmar, J W Moodie
{"title":"Preparative immunoabsorption electrophoresis. 1978.","authors":"A Polson, M B von Wechmar, J W Moodie","doi":"10.1080/10826069308544537","DOIUrl":"https://doi.org/10.1080/10826069308544537","url":null,"abstract":"ABSTRACT A method for separating a component from a mixture of antigens is described. The component, which may be a virus or subfraction of a virus, is isolated by driving the mixture by electrophoresis through a gel containing precipitating antibodies directed against the unwanted components. The method is illustrated by the isolation of hepatitis B antigen from whole serum and by the separation of wild cucumber mosaic virus from a strain of tobacco mosaic virus.","PeriodicalId":20391,"journal":{"name":"Preparative biochemistry","volume":"23 1-2","pages":"59-68"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10826069308544537","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19354428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Tube inserts as aids in preparative ultracentrifugation. 1983. 试管插入物辅助制备超离心。1983.
Preparative biochemistry Pub Date : 1993-02-01 DOI: 10.1080/10826069308544542
A Polson, K J van der Merwe
{"title":"Tube inserts as aids in preparative ultracentrifugation. 1983.","authors":"A Polson, K J van der Merwe","doi":"10.1080/10826069308544542","DOIUrl":"https://doi.org/10.1080/10826069308544542","url":null,"abstract":"","PeriodicalId":20391,"journal":{"name":"Preparative biochemistry","volume":"23 1-2","pages":"109-20"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10826069308544542","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19354419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation of the small infective particle in adapted MEF1 poliomyelitis. 1957. 适应性MEF1型脊髓灰质炎小感染颗粒的分离。1957.
Preparative biochemistry Pub Date : 1993-02-01 DOI: 10.1080/10826069308544547
A Polson, G Selzer
{"title":"Isolation of the small infective particle in adapted MEF1 poliomyelitis. 1957.","authors":"A Polson, G Selzer","doi":"10.1080/10826069308544547","DOIUrl":"https://doi.org/10.1080/10826069308544547","url":null,"abstract":"","PeriodicalId":20391,"journal":{"name":"Preparative biochemistry","volume":"23 1-2","pages":"159-65"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10826069308544547","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19382004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A theory for the displacement of proteins and viruses with polyethylene glycol. 1977. 用聚乙二醇取代蛋白质和病毒的理论。1977.
Preparative biochemistry Pub Date : 1993-02-01 DOI: 10.1080/10826069308544535
A Polson
{"title":"A theory for the displacement of proteins and viruses with polyethylene glycol. 1977.","authors":"A Polson","doi":"10.1080/10826069308544535","DOIUrl":"https://doi.org/10.1080/10826069308544535","url":null,"abstract":"","PeriodicalId":20391,"journal":{"name":"Preparative biochemistry","volume":"23 1-2","pages":"31-50"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10826069308544535","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18693169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation of a cytoplasmic polyhedrosis virus by physical and immunological techniques. 1975. 用物理和免疫学技术分离细胞质多角体病毒。1975.
Preparative biochemistry Pub Date : 1993-02-01 DOI: 10.1080/10826069308544552
R Rubinstein, L Stannard, A Polson
{"title":"Isolation of a cytoplasmic polyhedrosis virus by physical and immunological techniques. 1975.","authors":"R Rubinstein, L Stannard, A Polson","doi":"10.1080/10826069308544552","DOIUrl":"https://doi.org/10.1080/10826069308544552","url":null,"abstract":"","PeriodicalId":20391,"journal":{"name":"Preparative biochemistry","volume":"23 1-2","pages":"227-36"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10826069308544552","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19354423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Purification of filamentous plant viruses by thin layer centrifugation (applied to TMV, SCMV, PVX, SCV and YMC viruses). 1975. 丝状植物病毒的薄层离心纯化(适用于TMV、SCMV、PVX、SCV和YMC病毒)。1975.
Preparative biochemistry Pub Date : 1993-02-01 DOI: 10.1080/10826069308544553
A Polson, A Kiefer
{"title":"Purification of filamentous plant viruses by thin layer centrifugation (applied to TMV, SCMV, PVX, SCV and YMC viruses). 1975.","authors":"A Polson, A Kiefer","doi":"10.1080/10826069308544553","DOIUrl":"https://doi.org/10.1080/10826069308544553","url":null,"abstract":"ABSTRACT The construction of a modified thin layer ultracentrifuge rotor is described. This rotor was used in the purification of five filamentous plant viruses, viz. TMV, SCMV, PVX, SCV and YMC. The purification and concentration of these viruses in their monomeric forms is hazardous when conventional \"tube\" rotors are used since they invariably result in dissociation and aggregation of the virus particles. Using the thin layer rotor these infective agents may be concentrated in volumes of fluid equal to approximately 1% of the starting suspension and not as pellets obtained after ultracentrifugation in conventional \"tube\" rotors. Electron microscopy revealed that the virus particles concentrated by thin layer centrifugation were not aggregated and that only few fragments of the virus filaments were present in the final preparations.","PeriodicalId":20391,"journal":{"name":"Preparative biochemistry","volume":"23 1-2","pages":"237-53"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10826069308544553","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19354425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Purification of the neurite outgrowth promoting fragment of mouse laminin. 小鼠层粘连蛋白神经突生长促进片段的纯化。
Preparative biochemistry Pub Date : 1992-09-01 DOI: 10.1080/10826069208021373
S R Line
{"title":"Purification of the neurite outgrowth promoting fragment of mouse laminin.","authors":"S R Line","doi":"10.1080/10826069208021373","DOIUrl":"https://doi.org/10.1080/10826069208021373","url":null,"abstract":"<p><p>A method for isolation of the neurite outgrowth promoting fragment of mouse laminin (fragment 8) is described in this paper. Besides producing excellent yields, this method was shown to be fast and practical, since it is based on a single step which consists in an ion exchange chromatography of elastase digested laminin.</p>","PeriodicalId":20391,"journal":{"name":"Preparative biochemistry","volume":"22 3-4","pages":"229-37"},"PeriodicalIF":0.0,"publicationDate":"1992-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10826069208021373","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12462486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Partial purification of a receptor for the adipokinetic hormones from Musca autumnalis face flies. 秋蝇脂动激素受体的部分纯化。
Preparative biochemistry Pub Date : 1992-09-01 DOI: 10.1080/10826069208021372
N M Minnifield, D K Hayes
{"title":"Partial purification of a receptor for the adipokinetic hormones from Musca autumnalis face flies.","authors":"N M Minnifield,&nbsp;D K Hayes","doi":"10.1080/10826069208021372","DOIUrl":"https://doi.org/10.1080/10826069208021372","url":null,"abstract":"<p><p>Partial purification of the receptors for the neurohormones, diptera corpora cardiaca factors 1 and 2 (DCC1 and DCC2) was achieved. Receptor proteins were obtained from the abdomens of face fly, Musca autumnalis De Geer. Purification methods included detergent solubilization, affinity chromatography, and polyacrylamide gel electrophoresis. Analysis by gel electrophoresis has identified two proteins from this partial purification with relative molecular weights of 45 and 90 kD. A crude receptor preparation was used to develop a ligand binding assay with radiolabeled (tritiated and iodinated) DCC1. Ligand binding was inhibited by 90% when excess unlabeled DCC1 was added to the assay mixture. Ligand binding was optimum at pH 7.5. Binding saturation occurred at approximately 12 picomole radiolabeled ligand concentration. Because DCC1 and DCC2 have been shown to effect the lipid and trehalose levels in the insect an understanding of the neuropeptide-receptor interaction is important for the development of new methods of control of dairy and poultry muscoid flies.</p>","PeriodicalId":20391,"journal":{"name":"Preparative biochemistry","volume":"22 3-4","pages":"215-28"},"PeriodicalIF":0.0,"publicationDate":"1992-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10826069208021372","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12536389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：604180095
Book学术官方微信