发布求助
文献互助 智能选刊 最新文献

Preparative biochemistry最新文献

筛选
英文 中文
Bead centrifuge tube inserts--a semi-quantitative theory. 1984. 头离心管插入-半定量理论。1984.
Preparative biochemistry Pub Date : 1993-02-01 DOI: 10.1080/10826069308544543
A Polson, K J van der Merwe
{"title":"Bead centrifuge tube inserts--a semi-quantitative theory. 1984.","authors":"A Polson, K J van der Merwe","doi":"10.1080/10826069308544543","DOIUrl":"https://doi.org/10.1080/10826069308544543","url":null,"abstract":"A semi-quantitative theory is proposed for the sedimentation of particles in centrifuge tubes filled with polymer beads acting as sedimentation aids. The theory concerns the attainment of approximate sedimentation-diffusion equilibrium in minimum time of centrifugation. It is shown empirically that the time to reach this state is approximately proportional to the diameter of the polymer beads as predicted by the theory.","PeriodicalId":20391,"journal":{"name":"Preparative biochemistry","volume":"23 1-2","pages":"121-6"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10826069308544543","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19354420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation of viruses by electro-extraction of infected plants. 1977. 用电萃取法分离感染植物的病毒。1977.
Preparative biochemistry Pub Date : 1993-02-01 DOI: 10.1080/10826069308544536
A Polson
{"title":"Isolation of viruses by electro-extraction of infected plants. 1977.","authors":"A Polson","doi":"10.1080/10826069308544536","DOIUrl":"https://doi.org/10.1080/10826069308544536","url":null,"abstract":"","PeriodicalId":20391,"journal":{"name":"Preparative biochemistry","volume":"23 1-2","pages":"51-7"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10826069308544536","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19354427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A rapid method for the purification of the MEF1 strain of poliomyelitis virus. 1954. 一种快速纯化脊髓灰质炎病毒MEF1株的方法。1954.
Preparative biochemistry Pub Date : 1993-02-01 DOI: 10.1080/10826069308544545
A Polson, G Selzer
{"title":"A rapid method for the purification of the MEF1 strain of poliomyelitis virus. 1954.","authors":"A Polson, G Selzer","doi":"10.1080/10826069308544545","DOIUrl":"https://doi.org/10.1080/10826069308544545","url":null,"abstract":"","PeriodicalId":20391,"journal":{"name":"Preparative biochemistry","volume":"23 1-2","pages":"143-8"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10826069308544545","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19382002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Studies on poliomyelitis virus--concentration and purification of the virus. 1957. 脊髓灰质炎病毒的研究——病毒的浓缩和纯化。1957.
Preparative biochemistry Pub Date : 1993-02-01 DOI: 10.1080/10826069308544548
A Polson, J W Hampton
{"title":"Studies on poliomyelitis virus--concentration and purification of the virus. 1957.","authors":"A Polson, J W Hampton","doi":"10.1080/10826069308544548","DOIUrl":"https://doi.org/10.1080/10826069308544548","url":null,"abstract":"","PeriodicalId":20391,"journal":{"name":"Preparative biochemistry","volume":"23 1-2","pages":"167-70"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10826069308544548","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19382005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Special issue on virus separation and purification methods. 病毒分离和纯化方法特刊。
Preparative biochemistry Pub Date : 1993-02-01
{"title":"Special issue on virus separation and purification methods.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":20391,"journal":{"name":"Preparative biochemistry","volume":"23 1-2","pages":"1-284"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19354417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An insert for the reorienting gradient rotor for virus extraction from infected plants. 1979. 用于从受感染植物中提取病毒的重定向梯度转子的插入件。1979.
Preparative biochemistry Pub Date : 1993-02-01 DOI: 10.1080/10826069308544538
A Polson
{"title":"An insert for the reorienting gradient rotor for virus extraction from infected plants. 1979.","authors":"A Polson","doi":"10.1080/10826069308544538","DOIUrl":"https://doi.org/10.1080/10826069308544538","url":null,"abstract":"A device made of nylon and which is inserted in the bowl of the reorienting gradient rotor is described. This insert acts as a container for holding infected plant tissues for the purpose of separating virus by centrifugal force from intact and fresh plants, from frozen and thawed plants and from plant tissue disintegrated by mechanical means. The extracted fluid represents 80 to 90% of that present in the untreated plant tissue. Electron micrographs taken of concentrates of the viruses prepared by the centrifugation extraction procedure indicate that extraneous materials are reduced to a low level.","PeriodicalId":20391,"journal":{"name":"Preparative biochemistry","volume":"23 1-2","pages":"69-78"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10826069308544538","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19354429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigations of poliomyelitis antigens. 1958. 脊髓灰质炎抗原的调查。1958.
Preparative biochemistry Pub Date : 1993-02-01 DOI: 10.1080/10826069308544549
A Polson, A Ehrenberg, R Cramer
{"title":"Investigations of poliomyelitis antigens. 1958.","authors":"A Polson, A Ehrenberg, R Cramer","doi":"10.1080/10826069308544549","DOIUrl":"https://doi.org/10.1080/10826069308544549","url":null,"abstract":"The complex nature of the antigens in suckling mouse-adapted MEFi virus was indicated by Selzer and Poison1, who found that the infectivity was associated with two components of approximate sedimentation coefficients 100 and 173 S respectively. In addition to these components a “soluble antigen” was found which had a sedimentation coefficient of about 22 S. These results, which were obtained by using a Spinco preparative centrifuge (Poison and Linder2), have now been confirmed in the Spinco analytical ultracentrifuge of the Nobel Institute, Stockholm, and other types of poliomyelitis virus have been studied in both instruments.","PeriodicalId":20391,"journal":{"name":"Preparative biochemistry","volume":"23 1-2","pages":"171-90"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10826069308544549","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19382006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The sedimenting-droplet procedure for measuring fluid densities. 1980. 测量流体密度的沉淀液滴法。1980.
Preparative biochemistry Pub Date : 1993-02-01 DOI: 10.1080/10826069308544540
A Polson, H Alk, K Achtleitner
{"title":"The sedimenting-droplet procedure for measuring fluid densities. 1980.","authors":"A Polson, H Alk, K Achtleitner","doi":"10.1080/10826069308544540","DOIUrl":"https://doi.org/10.1080/10826069308544540","url":null,"abstract":"An apparatus is described which is used for the determination of velocities of falling droplets of small volumes (5/xl) through hydrophobic mixtures. From calibration curves with sodium chloride solutions of different molarities and specific gravities, the densities of the fluid in the droplets were obtained by interpolation. The density of the solutions of proteins may be determined and used in the calculation of partial specific volumes. The hydrophobic fluid used was a mixture of Dow Corning 200, kerosine to reduce the viscosity, and 1.2-dichlorobenzine to increase the specific gravity to approximately that of water.","PeriodicalId":20391,"journal":{"name":"Preparative biochemistry","volume":"23 1-2","pages":"81-92"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10826069308544540","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19354430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Determination of the number of Fab1 pieces which react with high molecular weight antigens. 1982. 测定与高分子量抗原发生反应的Fab1片段数目。1982.
Preparative biochemistry Pub Date : 1993-02-01 DOI: 10.1080/10826069308544541
A Polson, K J van der Merwe
{"title":"Determination of the number of Fab1 pieces which react with high molecular weight antigens. 1982.","authors":"A Polson, K J van der Merwe","doi":"10.1080/10826069308544541","DOIUrl":"https://doi.org/10.1080/10826069308544541","url":null,"abstract":"","PeriodicalId":20391,"journal":{"name":"Preparative biochemistry","volume":"23 1-2","pages":"93-107"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10826069308544541","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19352987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Purification of viruses by centrifugation in gradients of inert substances. 用惰性物质梯度离心纯化病毒。
Preparative biochemistry Pub Date : 1993-02-01 DOI: 10.1080/10826069308544544
A Polson
{"title":"Purification of viruses by centrifugation in gradients of inert substances.","authors":"A Polson","doi":"10.1080/10826069308544544","DOIUrl":"https://doi.org/10.1080/10826069308544544","url":null,"abstract":"<p><p>By using a reorienting gradient centrifuge rotor cut from a block of Nylon and fitted with eight septae, it was possible to separate the components of the haemolymph of the mollusc Turbo sarmaticus into three fractions in a sucrose gradient held in the bowl of the rotor. The fractions were (108 and 98)S, 44S and 16-22S. The success of the experiment was due to the large differences in the sedimentation coefficients of the components. When the rotor was applied to the natural mixture of the five viruses of the caterpillars of Nudaurelia cytheria only the main component could be isolated in a pure state. The viruses were separated by isopycnic centrifugation in \"self formed\" caesium chloride gradients, using a Beckman Model E analytical centrifuge in which a separation cell fitted with a centerpiece with two perforated partitions was used.</p>","PeriodicalId":20391,"journal":{"name":"Preparative biochemistry","volume":"23 1-2","pages":"127-40"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10826069308544544","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19354421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：604180095
Book学术官方微信