Porcine Health Management最新文献

{"title":"Characterization of the enteric virome of clinically healthy pigs around weaning on commercial farms in the Netherlands using next generation sequencing and qPCR.","authors":"M A R Schyns, R van den Braak, J Peijnenborg, S Coppens, M Deijs, M G J M Burggraaff, W I Kuller, S Theuns, L van der Hoek, A de Groof","doi":"10.1186/s40813-025-00446-5","DOIUrl":"https://doi.org/10.1186/s40813-025-00446-5","url":null,"abstract":"<p><strong>Background: </strong>Enteric virus infections around time of weaning have always been related to pig diseases such as postweaning diarrhea. Little, however, is known about the virus infection pattern (species, timing and viral load) in clinically healthy pigs. Virus infections may help to train and shape the immune system and presumably only lead to clinical disease when uncontrolled. Next Generation Sequencing (NGS) is a relatively new technique that can uncover the composition of the enteric virome. This study describes the dynamics of the enteric virome in clinically healthy pigs using NGS and qPCR until 10 weeks of age.</p><p><strong>Methods: </strong>Seven farms were selected based on the following criteria: diarrhea after weaning was visible in less than 5% of the pens, piglets reached 25 kg of body weight before 10 weeks of age and no antimicrobial batch treatment had been used on the farm for the last six months. Rectal swabs were taken in five different age groups: 2, 3.5, 5, 7 and 10 weeks of age, 10 piglets per age group, in a cross-sectional setup. Two NGS platforms were used to detect enteric viruses. Eleven virus-specific qPCRs were used to corroborate the results of the NGS analyses.</p><p><strong>Results: </strong>Rotavirus A, Porcine Kobuvirus, Enterovirus G and Porcine Astrovirus 3 and 4 were first detected at two weeks of age, followed by detection of Porcine Astrovirus 5 at 3.5 weeks of age, just before weaning. One week after weaning, at 5 weeks of age, Porcine Astrovirus 3 was undetectable, but now Porcine Astrovirus 1 and 2 had successively made their entry. Although Rotavirus B & C, Porcine Sapelovirus and Porcine Sapovirus were already detected just before weaning, the amount of virus peaked one week after weaning. Rotavirus H was first detected one week after weaning and peaked at 7 weeks of age. Many viruses were cleared by the age of 10 weeks.</p><p><strong>Conclusions: </strong>The timing and magnitude of subclinical enteric virus infections across farms were remarkably similar. Our study offers insight into the dynamics of enteric virome development in healthy pigs and provides essential context to NGS-based diagnostics.</p>","PeriodicalId":20352,"journal":{"name":"Porcine Health Management","volume":"11 1","pages":"41"},"PeriodicalIF":3.0,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144708555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autogenous Streptococcus suis serotype 1 bacterin: immunogenicities of sow and gilt vaccination protocols.","authors":"Georg Freymüller, Silke Lehnert, Christine Unterweger, Thomas Voglmayr, Christoph G Baums, Leonie Mayer","doi":"10.1186/s40813-025-00453-6","DOIUrl":"10.1186/s40813-025-00453-6","url":null,"abstract":"","PeriodicalId":20352,"journal":{"name":"Porcine Health Management","volume":"11 1","pages":"40"},"PeriodicalIF":3.0,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12275323/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144668135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial use in sows in Finland and its association with herd characteristics.","authors":"Kristina Ahlqvist, Camilla Munsterhjelm, Anna-Riia Holmström, Minna Kujala-Wirth, Vera Talvitie, Anna Valros, Mari Heinonen","doi":"10.1186/s40813-025-00447-4","DOIUrl":"10.1186/s40813-025-00447-4","url":null,"abstract":"","PeriodicalId":20352,"journal":{"name":"Porcine Health Management","volume":"11 1","pages":"39"},"PeriodicalIF":3.0,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12261644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of PCV2 vaccine immunogenicity and efficacy using ELISpot to detect virus-specific memory B cells.","authors":"Jie Fan, Fangcheng Fan, Zhixiong Chen, Ping Chen, Yanli Zhu, Xin Li, Tiantian Liu, Runcheng Li, Wei Dong, Meng Ge","doi":"10.1186/s40813-025-00452-7","DOIUrl":"10.1186/s40813-025-00452-7","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Porcine Circovirus 2 (PCV2) vaccination plays a crucial role in preventing porcine circovirus-associated disease (PCVAD). Nevertheless, pig farms face significant challenges in evaluating vaccination efficacy due to the inability of PCV2 vaccines to achieve sterilizing immunity and the variability among vaccine manufacturers. These challenges are further compounded by the limitations of conventional antibody detection methods, which fail to distinguish between maternally-derived antibodies (MDAs) and vaccine-induced antibodies. The accurate evaluation and selection of PCV2 vaccines is critical for the swine industry. The present study aimed to develop an Enzyme-linked immunospot (ELISpot) assay for directly detecting PCV2-specific memory B cells. This approach was used to assess the presence of PCV2-specific memory B cells in piglets with high levels of MDA vaccinated with different PCV2 vaccines, thus enabling the evaluation of vaccine immunogenicity at the cellular level. Furthermore, antibody levels and the viremia status were analyzed using Enzyme-linked immunosorbent assay (ELISA) and quantitative real-time polymerase chain reaction (qPCR) respectively to provide a comprehensive assessment of the ELISpot assay potential for evaluating the vaccine immunogenicity of PCV2 vaccines.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;The findings revealed that the optimal conditions for the developed ELISpot assay included stimulation with R848 at a final concentration of 1 µg·mL⁻¹ for three days, a PCV2 Cap protein coating concentration of 1.25 µg·mL⁻¹, a biotinylated goat anti-pig IgG antibody concentration of 5 µg·mL⁻¹, and an HRP-streptavidin concentration of 0.25 µg·mL⁻¹. In high MDA piglets immunized with different vaccines, serum antibody detection showed that PCV2 antibody levels declined continuously over time in all vaccinated and saline-injected control groups, demonstrating similar trends. In contrast, ELISpot analysis demonstrated a significant increase in PCV2-specific memory B cell levels in all three vaccinated groups compared to the saline-injected group. Among the vaccines tested, Vaccine A induced the highest levels of specific memory B cells, followed by Vaccine B. This was consistent with the lower PCV2 infection rates and viremia levels observed in Vaccine A and Vaccine B groups, compared to Vaccine C and saline-injected control groups.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;We established an ELISpot assay to quantify PCV2-specific memory B cells, revealing that vaccinated piglets with high MDA levels developed robust memory B cell responses. However, levels of PCV2 IgG antibodies in vaccinated piglets remained statistically indistinguishable from control piglets. These findings demonstrate that ELISpot-based profiling of PCV2-specific memory B cells overcomes the confounding effects of MDA in vaccine efficacy assessments. This approach reliably reflects the humoral immune response induced by vaccination and its re","PeriodicalId":20352,"journal":{"name":"Porcine Health Management","volume":"11 1","pages":"38"},"PeriodicalIF":3.0,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12247238/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144609182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary supplementation of Galactooligosaccharides improves growth performance and intestinal barrier function of offspring.","authors":"Jichang Zheng, Haibo Shen, Bi'e Tan, Yulong Yin, Jing Wang","doi":"10.1186/s40813-025-00437-6","DOIUrl":"10.1186/s40813-025-00437-6","url":null,"abstract":"<p><p>This study aims to investigate the effects of maternal supplementation with galactooligosaccharides (GOS) on intestinal digestion, absorption, and barrier function of offspring. Twenty four late-pregnant Ningxiang sows were randomly assigned into 3 treatments, including basic diet, basic diet with 0.5% GOS, basic diet with 100 mg/kg aureomycin, 20mg/kg ciprofloxacin and 10mg/kg vancomycin. The results showed that maternal dietary supplementation of GOS increased the body weight and average daily gain, reduced the diarrhea score of piglets, and increased the activities of digestive enzymes including maltase and α-amylase. Maternal GOS improved the intestinal barrier integrity and function of piglets, which exhibiting with increased ileal ratio of villus to crypt, and higher expressions of secretory immunoglobulin A, tight junction protein and mucin-2 in ileum. Maternal GOS treatment increased the contents of anti-inflammatory factors IL-4, IL-10 and TGF-β, but decreased the contents of pro-inflammatory factors IL-1β, IL-6 in the ileum and colon of piglets. In addition, piglets in maternal GOS treatment had increased abundance of probiotics in the colonic contents, as well as higher short-chain fatty acids concentrations in the colonic and cecal contents. Overall, this study provides data support for the nutritional regulation of maternal supplementation with 0.5% GOS to promote intestinal health of offspring.</p>","PeriodicalId":20352,"journal":{"name":"Porcine Health Management","volume":"11 1","pages":"37"},"PeriodicalIF":3.0,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12228311/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144565102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: from Brambell to beyond: advancing pig welfare through the five freedoms framework.","authors":"Mari Heinonen, Elisabeth Grosse Beilage","doi":"10.1186/s40813-025-00449-2","DOIUrl":"10.1186/s40813-025-00449-2","url":null,"abstract":"","PeriodicalId":20352,"journal":{"name":"Porcine Health Management","volume":"11 1","pages":"36"},"PeriodicalIF":3.0,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal evolution of Sow herd age structure and its impact on the performance of Spanish commercial farms.","authors":"S Sanz-Fernández, C Díaz-Gaona, J Simões, J C Casas-Rosal, N Alòs, L Tusell, R Quintanilla, Vicente Rodríguez-Estévez","doi":"10.1186/s40813-025-00451-8","DOIUrl":"10.1186/s40813-025-00451-8","url":null,"abstract":"<p><strong>Background: </strong>Efficient herd management is crucial for maintaining constant pig production. This study analysed the evolution of census structure over time and how productive performance variables related to farm efficiency are affected and evolved, using data from 427 Spanish commercial pig farms over three years (2020-2022). Farms were classified into three types of herd age structures based on the first coefficient of a quadratic function representing sow parity distribution. A longitudinal analysis was performed to evaluate changes in herd age structure and productivity over time, applying repeated measures ANOVA.</p><p><strong>Results: </strong>The herd age structure types in 2020 were: HS1 (with a downward-concave trend), HS2 (with a trend close to a straight line), and HS3 (with an upward-concave trend). HS1 farms had the highest productivity over time, maintaining superior performance compared to HS2 and HS3 (p < 0.01), with 31.4 piglets weaned per sow per year in 2021 and 30.9 in 2022. However, HS1 farms showed moderate consistency in herd parity structure over time, with 48.6% remaining in the same group in 2021 and 43% in 2022. HS2 farms showed the greatest herd parity structure stability over time, with 60.1% and 54.5% of farms remaining in this group in 2021 and 2022, respectively. HS3 farms were the least stable, with only 29% retaining their classification by 2022, and had the lowest productivity.</p><p><strong>Conclusions: </strong>Classifying farms by herd age structure provides valuable insights into how parity distribution influences farm productivity over time and how herd parity structure evolves. HS1 farms achieved the best productivity over the study but require specific management practices to maintain their stability. HS2 remained with the most stable herd structure over time, with intermediate productivity, while HS3 farms showed the worst stability and performance. Thus, HS1 is recommended as the optimal herd age structure for maximizing productivity in the short and medium term. Further research should focus on identifying specific management factors to optimize productivity and ensure long-term herd structure stability of HS1.</p>","PeriodicalId":20352,"journal":{"name":"Porcine Health Management","volume":"11 1","pages":"35"},"PeriodicalIF":3.0,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12180210/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound examination of the uterine diameter and the uterine blood vessels size in temporarily confined sows in the postpartal period- a pilot study.","authors":"Tip-Apa Akkhaphan, Preechaphon Taechamaeteekul, Alexander Grahofer, Padet Tummaruk","doi":"10.1186/s40813-025-00448-3","DOIUrl":"10.1186/s40813-025-00448-3","url":null,"abstract":"<p><strong>Background: </strong>Prolonged farrowing duration is currently one of the challenges in the swine industry, which leads to postpartum complications by increasing uterine inflammation, and interference with physiological processes after parturition. The aim of the present study was to assess the relationship between uterine involution and uterine vessel size with farrowing duration in temporarily confined sows (during the day of farrowing and for the following 3 days) under tropical climates.</p><p><strong>Results: </strong>Fourteen Landrace × Yorkshire sows were included in the study. Uterine diameter and uterine vessel size were measured from 2 to 15 days postpartum using B-mode ultrasonography, a non-invasive technique that enables continuous monitoring of uterine involution and detection of reproductive problems in the same sows throughout the 15-day postpartum period. To determine the relationship between farrowing duration and uterine diameter, sows were categorized into two groups: sows with a farrowing duration of ≤ 300 min (normal) and sows with a farrowing duration > 300 min (prolonged). The mean farrowing duration was 200 ± 128 min, with 28.6% of sows experiencing prolonged farrowing. In the prolonged farrowing group, the diameter decreased from 43.5 ± 9.4 mm to 12.4 ± 0.5 mm, while in the normal group, it decreased from 30.7 ± 1.4 mm to 13.6 ± 0.4 mm. On day 2, sows in the prolonged farrowing group exhibited a higher uterine diameter than the normal group (P = 0.042). There was no significant difference in the size of the uterine blood vessels between the two groups (prolonged farrowing: 7.3 ± 1.3 mm to 2.5 ± 0.1 mm; normal farrowing 6.1 ± 0.7 mm to 2.7 ± 0.1 mm) (P = 0.397). However, the uterine diameter was correlated with the size of the uterine blood vessels in sows with either a normal farrowing duration (r = 0.705, P < 0.001) or a prolonged farrowing duration (r = 0.749, P < 0.001).</p><p><strong>Conclusions: </strong>This is the first study to evaluate and provide initial data on uterine vessel size and its correlation with the uterine diameter in postpartum sows. Although no significant differences between uterine vessel size and farrowing duration were detected, a notable increase in uterine size during the initial 2 days postpartum in temporarily confined sows under tropical climates with prolonged farrowing duration was identified.</p>","PeriodicalId":20352,"journal":{"name":"Porcine Health Management","volume":"11 1","pages":"33"},"PeriodicalIF":3.0,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12153129/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zoonotic rat hepatitis E virus infection in pigs: farm prevalence and public health relevance.","authors":"Javier Caballero-Gómez, Ignacio García-Bocanegra, David Cano-Terriza, María Casares-Jiménez, Saúl Jiménez-Ruiz, María A Risalde, Lucía Ríos-Muñoz, Antonio Rivero-Juárez, Antonio Rivero","doi":"10.1186/s40813-025-00450-9","DOIUrl":"10.1186/s40813-025-00450-9","url":null,"abstract":"<p><p>Rat hepatitis E virus (ratHEV) is an emerging zoonotic pathogen causing acute and chronic hepatitis, with zoonotic transmission pathways that remain unclear. While rats are its primary reservoir, recent experimental studies revealed that pigs are susceptible to ratHEV infection. To better understand its epidemiology, we assessed the herd prevalence of ratHEV in 64 extensively managed pig farms in southwestern Spain. Serum samples from 1,861 animals were pooled and analyzed using a multiplex qPCR. Viral RNA was detected in 9 (1.9%) of the 466 analyzed pools, with a herd prevalence of 10.9% (7/64; 95%CI: 5.4-20.9). Positive farms were distributed across the study area, and ratHEV circulation was limited to fattening farms (17.5%) (P = 0.007). These findings suggest pigs could act as intermediate hosts between rats and humans, posing a public health risk. Enhanced surveillance in pig farms and pork products is crucial to assess zoonotic transmission risks.</p>","PeriodicalId":20352,"journal":{"name":"Porcine Health Management","volume":"11 1","pages":"34"},"PeriodicalIF":3.0,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12160372/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and possibility of vertical transmission of G9P[23] and G12P[7] group A rotavirus strains in pigs.","authors":"Yang Li, Chunliu Gao, Lili Wu, Jie Qing, Minxia Zhang, Mengli Qiao, Zhiqiang Hu, Bingzhou Zhang, Chen Yang, Zewei Wang, Lulu Li, Zheng Yan, Weisheng Wu, Wei Liu, Jing Ren, Xiaowen Li","doi":"10.1186/s40813-025-00445-6","DOIUrl":"10.1186/s40813-025-00445-6","url":null,"abstract":"<p><strong>Background: </strong>Porcine group A rotavirus (RVA) is a significant causative agent of diarrhea in piglets, leading to substantial economic losses in pig farms worldwide. While horizontal transmission of RVA among pig populations is well documented, the possibility of vertical transmission from sows to newborn piglets has not been definitively confirmed.</p><p><strong>Results: </strong>In this study, piglet testicles, umbilical cord blood, and colostrum were collected from porcine RVA (PoRVA)-active farms for analysis. The samples presented high PoRVA-positive rates, with 70.00% in the testicle samples, 55.00% in the umbilical cord blood samples, and 73.33% in the colostrum samples. Immunohistochemical assays confirmed the presence of PoRVA in neonatal piglet testicles. Additionally, two PoRVA strains, RVA/Pig/CHN/QT/2023/G9P [23] (QT2023) and RVA/Pig/CHN/BH/2023/G12P [7] (BH2023), were isolated from newborn piglet testicles. Complete genome analyses revealed that strains QT2023 and BH2023 both presented a Wa-like backbone, with the genotype constellation of G9-P [23]-I5-R1-C1-M1-A8-N1-T1-E1-H1 and G12-P [7]-I5-R1-C1-M1-A8-N1-T1-E1-H1, respectively. While strains QT2023 and BH2023 originated from PoRVAs, sequence identities and phylogenetic analyses suggested close relationships with human rotaviruses in specific genes. Furthermore, successful viral replication of these strains in MA-104 cells was observed. Inoculation of PoRVA-negative piglets with strains QT2023 and BH2023 resulted in clinical diarrhea, fecal virus shedding, and intestinal pathological changes, highlighting the pathogenicity of these strains.</p><p><strong>Conclusion: </strong>This study provides evidence that PoRVA can breach the placental barrier and spread to newborn piglets through vertical transmission. These discoveries enhance our understanding of the transmission route of porcine RVA and have the potential to guide the development of efficient vaccine strategies for combating this disease.</p>","PeriodicalId":20352,"journal":{"name":"Porcine Health Management","volume":"11 1","pages":"32"},"PeriodicalIF":3.0,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12144683/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}