Porcine Health Management最新文献

{"title":"Experimental efficacy of vaccination of weaned piglets with a modified-live commercial PRRS virus vaccine against the challenge with a Spanish highly virulent PRRSV-1 strain.","authors":"M Cortey, M Jiménez, L Aguirre, J M Sánchez-Carvajal, J Gómez-Laguna, I Domingo-Carreño, H Clilverd, M Marcos, R Menjon, S Von Berg, E Mateu","doi":"10.1186/s40813-025-00423-y","DOIUrl":"10.1186/s40813-025-00423-y","url":null,"abstract":"<p><strong>Background: </strong>In 2020, a highly virulent PRRSV-1 strain emerged in Spain and rapidly spread across the country. The purpose of the present study was to test in a piglet model whether a commercial PRRSV-1 modified live vaccine was able to confer protection against strain R1, a representative of the emerging clade. For that purpose, two groups of 26 piglets were either vaccinated intradermally or kept as controls; 42 days later, half of the animals in each group were intranasally challenged with the R1 strain. Then, animals were followed to assess the development of clinical signs (until 14 days post-challenge), lung lesions (10- and 35-days post-challenge), weight gains, viremia and nasal shedding and the immune response (anti PRRS virus nucleoprotein antibodies) by ELISA and virus specific-interferon-γ secreting cells by ELISPOT).</p><p><strong>Results: </strong>Challenge of naïve pigs resulted in high fever (up to 41.9 °C), lethargy and severely retarded growth (0.748 kg/day). In contrast, vaccinated/challenged pigs had less fever and for a shorter period, lower clinical scores and a higher average daily weight gain (0.940 kg/day), comparable to the unchallenged animals. At 10 days-post challenge, in naïve animals on average 49.1% of the lung was pneumonic (range 8-81%) while in vaccinated animals the average was 15.7% (4-41%). Duration of viremia was reduced in vaccinated animals and after 14 days post-challenge, most were negative by RT-qPCR. In contrast, 50% of the naïve/challenged pigs remained viremic at 35 days post-challenge. Vaccination induced rapid seroconversion and challenge of naïve animals resulted in 100% of ELISA-positive pigs by day 14 post-challenge. Regarding the development of IFN-γ responses, for vaccinated animals the frequencies increased until day 35 post-vaccination. After challenge, in vaccinated pigs, the peak of the R1-specific IFN-γ response was reached at 14 days and then the viremia ceased, although nasal shedding persisted in some vaccinated animals.</p><p><strong>Conclusions: </strong>In the present trial, vaccination resulted in improved clinical course, better weight gain and reduced viremia. At the peak of the infection, lung lesions were reduced in most animals although some individuals still had extensive pneumonia. In summary, vaccination was shown to provide partial but significant protection against the highly virulent R1 strain.</p>","PeriodicalId":20352,"journal":{"name":"Porcine Health Management","volume":"11 1","pages":"10"},"PeriodicalIF":3.0,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11846179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143477088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PRRSV-1 outbreak in a farrowing farm caused by a vaccine derived strain: a case report.","authors":"Arnaud Lebret, Patricia Renson, Mathieu Brissonnier, Céline Chevance, Valérie Normand, Justine Favrel, Jean-François Da-Costa, Justine Jeusselin, Théo Nicolazo, Yannick Blanchard, Olivier Bourry, Gwenaël Boulbria","doi":"10.1186/s40813-025-00425-w","DOIUrl":"10.1186/s40813-025-00425-w","url":null,"abstract":"<p><strong>Background: </strong>The benefits of porcine reproductive and respiratory syndrome (PRRS) modified live virus vaccines (MLV) have been largely proven, however, the safety of these vaccines is questioned since vaccine strains can revert to virulence due to random mutations or recombination events. Reversion to virulence has been previously described for PRRSV-2 MLVs and recently for PRRSV-1 MLV after recombination. This case report describes the introduction of a PRRSV-1 strain derived from a MLV associated with an outbreak of reproductive disorder in a 1000-sow farrow-to-wean farm in France.</p><p><strong>Case presentation: </strong>In January 2023, unusual fever and lethargy in sows, and premature farrowings were reported in a farm that was regularly controlled as PRRS stable, through mass vaccination of the sows. PRRSV-1 was detected by PCR in sows and suckling piglet samples. Sequencing of ORF5, ORF7, and whole genome (WGS) was performed. Time-to-baseline production and total production losses were calculated using statistical process control methods. ORF5 and ORF7 nucleotide sequences indicated that the strain isolated from the clinical samples was differentiable from the DV MLV strain used in the farm (94.1% and 95.9% respectively) but closely related to the VP-046 Bis MLV strain which was never used (99.0% and 99.2% respectively). WGS of the farm PRRSV strain confirmed the high nucleotide identity percentage with the VP-046 Bis MLV strain (98.6%) over the entire genome and no recombination events was detected with MLV strains authorized in France. After different investigations aiming to identify the source of contamination, we were able to detect a closely related strain (99.46% of identity with the case farm strain across the entire genome) in a wean-to-finish farm located 400 m further. It took 17 batches (34 weeks) to recover the baseline production of piglets after implementation of a PRRSV stabilization protocol, which represented a total loss of 812 weaned piglets.</p><p><strong>Conclusion: </strong>This is the first case report of a PRRSV-1 MLV which might have reverted to virulence in France and has caused substantial economic losses.</p>","PeriodicalId":20352,"journal":{"name":"Porcine Health Management","volume":"11 1","pages":"9"},"PeriodicalIF":3.0,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence estimation of Pestivirus scrofae (atypical porcine pestivirus) among Hungarian pig herds and the effects of different sample types on detection rates.","authors":"Lilla Dénes, Mihály Albert, Barbara Igriczi, Gyula Balka","doi":"10.1186/s40813-024-00416-3","DOIUrl":"10.1186/s40813-024-00416-3","url":null,"abstract":"<p><strong>Background: </strong>Atypical porcine pestivirus (APPeV), also known as Pestivirus scrofae, is a member of the Pestivirus genus within the Flaviviridae family. Experimental infections have directly linked APPeV to congenital tremor (CT) type A-II in congenitally infected piglets born to challenged sows. Here, we report the assessment of the prevalence of APPeV in Hungarian pig herds and the influence of different sample types on detection rates.</p><p><strong>Results: </strong>Altogether, 2650 blood serum, 198 oral fluid and 163 processing fluid samples were obtained via a systemic approach from 26 Hungarian farms and one Slovakian farm. The samples originated from different age groups and were analyzed via reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The estimated prevalence of APPeV was determined to be 66.67% in the sampled farms, indicating the widespread distribution of the virus within Hungary. Within the positive farms, APPeV genetic material was detected in the serum (21%), processing fluid (57%), and oral fluid (72%) samples. Notably, in some farms, the presence of APPeV was confirmed in only specific sample types, and five farms had APPeV in all three sample types. Age group analysis revealed that 10-week-old animals had the highest positivity rate in their blood serum (27%), whereas 20-week-old animals presented the highest rate in their oral fluid samples (59%). Processing fluid and oral fluid samples proved to be valuable for noninvasive diagnostic matrices, allowing for efficient population-level virus detection. We determined the partial NS2-3 coding region of 15 Hungarian strains and a Slovakian strain, and our phylogenetic analysis revealed that very similar strains can be found on different farms.</p><p><strong>Conclusion: </strong>In conclusion, our study provides insights into APPeV prevalence in Hungarian pig herds, emphasizing the importance of different sample types for accurate diagnostics. These findings contribute to our understanding of the virus's distribution across different age groups.</p>","PeriodicalId":20352,"journal":{"name":"Porcine Health Management","volume":"11 1","pages":"8"},"PeriodicalIF":3.0,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143425943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The kinetics of maternal and self-developed Streptococcus suis-specific antibodies.","authors":"Sandra Vreman, Rutger Jansen, Mikael Bastian, Patricia Beckers, Miriam van Riet, Helmi Fijten, Jan Fledderus, Astrid de Greeff, Hélène Winkelman, Norbert Stockhofe-Zurwieden, Lluís Fabà, Henk J Wisselink, Manouk Vrieling","doi":"10.1186/s40813-025-00422-z","DOIUrl":"10.1186/s40813-025-00422-z","url":null,"abstract":"<p><strong>Background: </strong>Streptococcus suis (S. suis) infections are responsible for a large disease burden in piglets during the nursery phase, compromising animal welfare and increasing antibiotic use. The immune gap caused by decreased maternal-derived antibodies (MDA) and insufficient levels or functionality of acquired antibodies in weaned pigs could play a role in the increased susceptibility to S. suis infections. To better understand this, two studies were performed. Study I evaluated the associations between sow antibodies in colostrum and serum, birth parameters (e.g., birth weight, colostrum intake and piglet growth) and the levels of S. suis-specific (serotypes 2 and 9) antibodies in one-day-old piglets from four farms. Subsequently, study II used one of these farms to evaluate S. suis-specific and total antibody kinetics in piglets (10 litters with 6 selected piglets per litter, total n = 60) from birth until 10 weeks of age. Additionally, tonsil swabs from sows and piglets were taken to evaluate the S. suis tonsillar carrier status (serotypes 2 and 9) before and after weaning.</p><p><strong>Results: </strong>High variability in serum and colostrum antibody levels was observed between and within the four farms (study I). In study II, there was a decrease in S. suis-specific MDA after 24 h of age, with the lowest level occurring at approximately 18/19 days of age. Afterwards, there was an increase in specific antibodies, most likely due to acquired immunity. Colostrum intake, birth weight and 24-h weight gain after birth were important parameters that were positively associated with S. suis antibody levels in piglets after birth but also affected these antibody levels at a later age. All the piglet tonsils were colonized with S. suis serotype 9 before weaning, while the prevalence of serotype 2 increased after weaning.</p><p><strong>Conclusions: </strong>Total Ig against S. suis in serum declined after birth and the lowest level was detected just before weaning. Farmers and veterinarians should focus on piglets with low birth weights and late-born piglets because these parameters reduce both the S. suis-specific MDA preweaning and the specific antibodies acquired postweaning. Colostrum intake and 24 h-weight gain also affect the level of S. suis specific antibodies on day 1.</p>","PeriodicalId":20352,"journal":{"name":"Porcine Health Management","volume":"11 1","pages":"7"},"PeriodicalIF":3.0,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11806565/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143371074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary steviol glycosides mixture supplementation modulates the gene expression of gut chemoreceptors and enhances the antioxidant capacity in weaned piglets.","authors":"Yunxia Xiong, Zhentao He, Qiwen Wu, Hao Xiao, Shuting Cao, Xuefen Yang, Yajing Li, Zongyong Jiang, Cui Zhu, Li Wang","doi":"10.1186/s40813-024-00414-5","DOIUrl":"10.1186/s40813-024-00414-5","url":null,"abstract":"<p><strong>Background: </strong>Stevia glycosides (SGs) have been widely used as an ideal sugar alternative in the food industry. However, the potential application of SGs mixture in the diets of weaned piglets remains unexplored. This study aimed to investigate the effect of dietary SGs mixture supplementation on growth performance, gene expression of gut chemoreceptors, and antioxidant capacity in weaned piglets.</p><p><strong>Methods: </strong>A total of 216 weaned piglets (Duroc × Landrace × Yorkshire, 7.36 ± 0.04 kg body weight) were randomly assigned to 6 groups (6 pens/group with 6 piglets/pen), and were fed with the basal diet supplemented with 0, 100, 150, 200, 250, or 300 mg/kg SGs mixture for 42 days. The serum, liver, longissimus thoracis, and jejunal samples were collected on day 43.</p><p><strong>Results: </strong>The results showed that inclusion the SGs mixture in the diet did not have a significant impact on growth performance from days 1 to 28 (P > 0.05). But increasing the concentration of SGs mixture tended to linearly decrease the average daily gain from days 1 to 42 (P = 0.052). However, 150 mg/kg SGs mixture supplementation significantly increased the mRNA expression of taste receptor family 1 member 2 (T1R2) and glucose transporters 2 (GLUT2) in the jejunum (P < 0.05), while 150 and 200 mg/kg SGs mixture supplementation significantly increased T1R3 mRNA expression (P < 0.05). Moreover, 150 mg/kg SGs mixture supplementation significantly reduced serum malondialdehyde content (P < 0.05). Increasing the concentration of SGs mixture linearly and quadratically increased serum total superoxide dismutase (T-SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activity, as well as hepatic T-SOD, GSH-Px activity, and muscle total antioxidant capacity contents (P < 0.05). Furthermore, piglets fed a diet supplemented with 100 mg/kg SGs mixture had higher serum T-SOD, CAT, and GSH-Px activities compared with the other treatments (P < 0.05).</p><p><strong>Conclusions: </strong>Therefore, our results suggest that dietary 100 ~ 150 mg/kg SGs mixture supplementation modulates gene expression of sweet taste recognition receptors and glucose transporters, while also enhancing the antioxidant capacity of weaned piglets.</p>","PeriodicalId":20352,"journal":{"name":"Porcine Health Management","volume":"11 1","pages":"6"},"PeriodicalIF":3.0,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11803942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Umbilical outpouchings in pigs - an international survey on fitness for transport, welfare and management.","authors":"Marie-Louise Hansen, Inge Larsen, Tina Birk Jensen, Charlotte Sonne Kristensen, Ken Steen Pedersen","doi":"10.1186/s40813-024-00411-8","DOIUrl":"10.1186/s40813-024-00411-8","url":null,"abstract":"<p><strong>Background: </strong>Umbilical outpouchings (UOs) in pigs are a multifactorial disease and little is known about effective prevention strategies and risk factors for UO development. UOs are common in Danish pigs and legislation complicates and increases the cost of keeping and raising pigs with UO. Recommendations for preventive measures exist but the scientific evidence behind the recommendations is often lacking. This study´s purpose was to investigate veterinarians' perspectives on UO pigs´ fitness for transport, the welfare of UO pigs, the significance of UO, risk factors for the development of UO, and the management of pigs with UO. This study´s purpose was to investigate veterinarians' perspectives on UO pigs´ fitness for transport, the welfare of UO pigs, the significance of UO, risk factors for the development of UO, and the management of pigs with UO.</p><p><strong>Results: </strong>The survey received 93 complete responses from veterinarians working within porcine health management. Because of the large proportion of replies from Danish veterinarians, all reporting of results was divided among Danish and non-Danish veterinarians. There were no statistically significant differences between the two groups in the assessment of fitness for transport. Both groups mainly agreed to a series of statements regarding the significance of UO and risk factors for UO development. The management of UO was also similar across the groups except for the use of neonatal antibiotics which was used considerably more in Danish herds.</p><p><strong>Conclusions: </strong>Umbilical outpouchings seem to be perceived as a challenge across pork production; affecting the welfare of the individual pig as well as the management of the entire production. There were no significant differences between Danish and non-Danish veterinarians' assessments of fitness for transport, and almost all the veterinarians agreed that some UO pigs might need special attention and care. Most would also recommend preventive measures. Despite most respondents in this survey working under the laws of the European Union, some were unaware of legislation regarding UO pigs.</p>","PeriodicalId":20352,"journal":{"name":"Porcine Health Management","volume":"11 1","pages":"4"},"PeriodicalIF":3.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11721061/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A proposal of a fecal scoring system based on physicochemical analyses of suckling pig feces.","authors":"Ana Maria Pons, Blanca González, Joan Pujols, Jordi Serratosa, Joaquim Segalés, Ernesto A Gómez, Jorge Martínez","doi":"10.1186/s40813-024-00417-2","DOIUrl":"10.1186/s40813-024-00417-2","url":null,"abstract":"<p><strong>Background: </strong>Digestive disorders are one of the main health problems in suckling piglets. The correct visual identification of feces in suckling piglets is an important tool for the diagnosis of enteric diseases. The aim of the present observational study was to analyze different physicochemical parameters of the feces of suckling piglets aged 0 to 21 days: visual appearance (color and consistency), fecal dry matter (FDM) content and pH. A total of 482 fecal samples were collected and visually classified into six categories: meconium, colostrum stage feces and 4 further scores according to the degree of consistency: 0 = form; 1 = pasty; 2 = liquid; and 3 = watery feces. The percentage of FDM was estimated by two drying methods, oven and microwave, doing duplicates in each one to evaluate methods, and both were compared.</p><p><strong>Results: </strong>The most frequent colors of each feces category were dark green or dark brown for meconium; orange for colostrum; formed feces were mostly ocher and for the rest of the feces, the colors varied predominantly cream and ocher. Regarding FDM, liquid and watery categories had no statistically significant differences between them; meconium and colostrum feces FDM were not statistically different from pasty feces. The correlation coefficient between the FDM values of the duplicate analyses of the samples by both methods (oven and microwave) was very high (> 0.988). Importantly, no differences were found while comparing the results between both methods (p = 0.078), and the correlation coefficient between all samples analyzed with both methods was very high (> 0.98). Meconium was the only one that differed significantly from the rest in terms of pH.</p><p><strong>Conclusion: </strong>The physicochemical study of the feces of suckling piglets including color, FDM and pH allowed the establishment of an objective fecal score to characterize the stools in this age group.</p>","PeriodicalId":20352,"journal":{"name":"Porcine Health Management","volume":"11 1","pages":"5"},"PeriodicalIF":3.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11716274/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serotype distribution and antimicrobial susceptibility of Streptococcus suis isolates from porcine diagnostic samples in Hungary, 2020-2023.","authors":"Ervin Albert, István Emil Kis, Krisztián Kiss, Katalin K-Jánosi, Tamás Révész, Imre Biksi","doi":"10.1186/s40813-024-00419-0","DOIUrl":"10.1186/s40813-024-00419-0","url":null,"abstract":"<p><strong>Background: </strong>Streptococcus suis (S. suis) is a major swine pathogen and a significant zoonotic agent, causing substantial economic losses in the swine sector and having considerable public health importance. The control and management of S. suis-related conditions has become increasingly challenging due to the multitude of involved serotypes with varying antimicrobial resistance patterns. Here, we report the serological distribution and antimicrobial susceptibility of S. suis isolates isolated form clinical samples of Hungarian large-scale swine farms.</p><p><strong>Results: </strong>Between 2020 and 2023, altogether 296 S. suis isolates were obtained from diseased pigs of 64 Hungarian pig operations. Serotyping of the isolates was carried out by using molecular methods (cps-typing). The isolated strains belonged to 24 single cps-types. The most frequently detected cps-types during the four years of this passive survey were 9 (19.6%), 2 (19.3%), 1/2 (18.9%) and 7 (14.5%). The brain, spleen, endocardial valve thrombus and lung proved to be the most frequent site of S. suis strain isolation, and animals 29-75 days of age were affected in the highest proportion. Antimicrobial susceptibility testing of the isolates was performed by determining the minimal inhibitory concentration for 15 antimicrobial agents of veterinary and human importance using a commercial microdilution assay. More than 90% of the tested isolates proved to be susceptible to the examined beta-lactams, cephalosporins and florfenicol, as well as to rifampicin, trimethoprim/sulfamethoxazole and vancomycin. Phenotypic resistance profiles (resistotypes) of clindamycin-tetracyclin (3.8%), clindamycin-erythromycin-tetracyclin (8.4%) and clindamycin-erythromycin-tetracyclin-trimethoprim / sulfamethoxazole (3.8%) were most frequently detected. Vancomycin resistance was observed in the case of 1 S. suis strain.</p><p><strong>Conclusions: </strong>The dominance of S. suis cps-types 9, 2, 1/2 and 7 in Hungary over the four years of this study aligns with previous reports from several countries worldwide. The presence of highly susceptible S. suis isolates suggests a prudent antibiotic usage and treatment practice in the surveyed Hungarian swine operations. In contrary, the presence of several resistotypes could indicate the problem of antibiotic resistance in the future.</p>","PeriodicalId":20352,"journal":{"name":"Porcine Health Management","volume":"11 1","pages":"3"},"PeriodicalIF":3.0,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11708007/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Field study on the suitability of oral fluid samples for monitoring of Lawsonia intracellularis and Brachyspira hyodysenteriae by multiplex qPCR under field conditions.","authors":"Matthias Eddicks, Gerald Reiner, Sigena Junker, Hermann Willems, Sabrina Becker, Julia Stadler, Josefine Hagn, Mathias Ritzmann","doi":"10.1186/s40813-024-00415-4","DOIUrl":"https://doi.org/10.1186/s40813-024-00415-4","url":null,"abstract":"<p><strong>Background: </strong>Monitoring or surveillance of infectious diseases is crucial in terms of herd health management of livestock. Investigations of oral fluids have become an animal friendly routine strategy to monitor respiratory pathogens in pigs. Less is known about the suitability of oral fluids for the detection of enteric pathogens in swine. In the present study we evaluated the use of oral fluids to monitor B. hyodysenteriae and L. intracellularis compared to pooled fecal samples by multiplex qPCR in a pen-wise follow-up of fattening pigs. Therefore, we collected oral fluids at an age of 12, 16 and 20 weeks of life and compared them to pooled fecal samples collected from the same pens on two fattening farms.</p><p><strong>Results: </strong>Cohen´s Kappa analysis revealed a substantial agreement between oral fluids and pooled fecal samples on pen level (Cohen´s Kappa: 0.745; p < 0.001). DNA-loads of L. intracellularis were tendentially higher (p = 0.053) in pooled fecal samples than in the corresponding OFs.</p><p><strong>Conclusions: </strong>The present study shows that oral fluids are an appropriate tool to monitor B. hyodysenteriae and L. intracellularis on conventional fattening farms under field conditions. However, multiple pen testing should be conducted to increase the diagnostic performance and sensitivity.</p>","PeriodicalId":20352,"journal":{"name":"Porcine Health Management","volume":"11 1","pages":"2"},"PeriodicalIF":3.0,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11706030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparison of two systems for group housing of gestating sows - effects on productivity, removal, and treatments.","authors":"Kaisa Ryytty Sylvén, Torun Wallgren, Pontus Almerheim, Lena Eliasson Selling, Magdalena Jacobson, Per Wallgren","doi":"10.1186/s40813-024-00410-9","DOIUrl":"https://doi.org/10.1186/s40813-024-00410-9","url":null,"abstract":"<p><strong>Background: </strong>Group housing of sows has been extensively studied since the EU banned gestation crating. Well-managed group-housing promotes sow welfare, but the impact varies based on factors such as feeding, group characteristics, and environmental features. Adequate floor space, particularly directly post-mixing, is crucial for social interactions, natural behaviours, and to reduce injuries caused by aggression. The aim of this study was to compare two group-housing systems for gestating sows with respect to productivity, treatment frequency, and removal of sows. Both systems were static but differed in space allowance, quantity of enrichment material and feeding management. System I comprised of large sized pens with deep litter straw bedding, housing in total 40 sows, and System II of smaller sized pens with permanent access to straw, housing 8 to 10 sows.</p><p><strong>Results: </strong>The mean parity number was 3.1 ± 1.3 in both groups. Sows housed in System I with large groups (n = 40) in large pens with deep litter straw gave birth to 16.8 ± 0.33 (Least Squares Means, LSM) piglets, compared to 15.4 ± 0.31 (LSM) for sows in System II kept in smaller groups (n = 8-10) in smaller pens (p = 0.0005). Medical treatments of sows were more frequent (p < 0.001) in System II. The incidence of replacement of sows was comparable in both systems, and there was a high occurrence of sows becoming pregnant during the subsequent insemination in both groups.</p><p><strong>Conclusions: </strong>This study indicated that sows kept in larger groups provided with a larger floor space (a total area of 156 m², corresponding to 3.9 m² per sow) and housed on deep straw had a higher number of liveborn and weaned piglets and lower incidence of antibiotic treatments than sows with less floor space (a total area of 24.5 m², corresponding to 2.5-3.1 m² per sow) and less bedding/manipulable material.</p>","PeriodicalId":20352,"journal":{"name":"Porcine Health Management","volume":"11 1","pages":"1"},"PeriodicalIF":3.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11706077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}