Hassan Abushanab, Aly I. Mohamed, J. Eljakee, A. Samir
{"title":"Snake venom as an antibacterial agent","authors":"Hassan Abushanab, Aly I. Mohamed, J. Eljakee, A. Samir","doi":"10.21608/vmjg.2019.156587","DOIUrl":"https://doi.org/10.21608/vmjg.2019.156587","url":null,"abstract":"","PeriodicalId":202817,"journal":{"name":"Veterinary Medical Journal (Giza)","volume":"370 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123060693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Minor groove binder probe real-time RT-PCR for detection of foot-and mouth disease virus in Egypt","authors":"H. Abu-Elnaga","doi":"10.21608/vmjg.2018.155932","DOIUrl":"https://doi.org/10.21608/vmjg.2018.155932","url":null,"abstract":"Shorter, more specific minor groove binders (MGBs) probes are dsDNA-binding agents attached to the 3' end of Taq Man probes that could be designed strictly to the invariant region. Application and assessing of a new trend for viral detection in Egypt depending on MGB probe real-time RT-PCR (rRT-PCR) applied on local FMDV serotypes O, A, and SAT2. Moreover, FMDV O was detected using two serotype specific primer sets by SYBR Green real-time RT-PCR assaying rapid formats. The limit of detection of diluted RNAs using MGB probe rRT-PCR assay reached to ≤ 6 FG/ul. Besides, the high specificity of it was clear. In contrary, the employing of FMDV O specific primer pairs in SYBR Green real-time RT-PCR showed less sensitivity and specificity, particularly one of them displayed poor performance illustrating important cause of the false negative results in the conventional PCR. Lastly, the local financial cost of MGB probe is considered the obvious hinder in my country.","PeriodicalId":202817,"journal":{"name":"Veterinary Medical Journal (Giza)","volume":"78 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130580084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Kafafy, S. El Soally, E. Aboul-Soud, M. Zaghloul, C. Mikhael
{"title":"Preparation of trivalent vaccine against lumpy skin disease using different capripox viral strain","authors":"M. Kafafy, S. El Soally, E. Aboul-Soud, M. Zaghloul, C. Mikhael","doi":"10.21608/vmjg.2018.155986","DOIUrl":"https://doi.org/10.21608/vmjg.2018.155986","url":null,"abstract":"","PeriodicalId":202817,"journal":{"name":"Veterinary Medical Journal (Giza)","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122478120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The use of ultasonication for preparation of nano-adjuvant rabies inactivated vaccine","authors":"Amany El-zieny, A. Soliman, S. ElDek, A. Farghali","doi":"10.21608/vmjg.2018.156217","DOIUrl":"https://doi.org/10.21608/vmjg.2018.156217","url":null,"abstract":"","PeriodicalId":202817,"journal":{"name":"Veterinary Medical Journal (Giza)","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122332409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Kafafy, E. Aboul-Soud, Olfat E. Nakhla, Christine Michael, Nermin El-Said
{"title":"Preliminary studies on preparation and evaluation of a local isolate tissue culture propagated pigeon pox vaccine","authors":"M. Kafafy, E. Aboul-Soud, Olfat E. Nakhla, Christine Michael, Nermin El-Said","doi":"10.21608/vmjg.2018.156213","DOIUrl":"https://doi.org/10.21608/vmjg.2018.156213","url":null,"abstract":"","PeriodicalId":202817,"journal":{"name":"Veterinary Medical Journal (Giza)","volume":"104 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134405353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Abu-Elnaga, S. Rizk, Hind Dauod, Akram Hegazy, W. Shabana
{"title":"Studies on the using of 2-Phenoxyethanol as an alternative to Thiomersal as a preservative in Foot-and-Mouth Disease vaccine","authors":"H. Abu-Elnaga, S. Rizk, Hind Dauod, Akram Hegazy, W. Shabana","doi":"10.21608/vmjg.2018.155935","DOIUrl":"https://doi.org/10.21608/vmjg.2018.155935","url":null,"abstract":"The progress in foot-and-mouth disease (FMD) vaccine production directed primarily towards the safety of the vaccine, purity of the antigen, selection of proper additives, as adjuvant and preservative. Thimerosal (Merthiolate) has been used as a preservative since 1930. Nevertheless, it is important to note that Thiomersal itself proved to be very toxic because it contains mercury. Hence, the current article discussed the cause and the prevention measures of the pyrogen-free colored sediment that might appear in the vaccine formula. Where the etiology might appear in the biological product was approached and solved. Besides, 2-phenoxyethanol examined as an alternative preservative in FMD vaccine, where it showed safety and efficacy as substitutional.","PeriodicalId":202817,"journal":{"name":"Veterinary Medical Journal (Giza)","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125077267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}