{"title":"Hematological, biochemical and histopathological studies on selected canine skin diseases","authors":"Soad A. Kamel, Osama M. Abdou, K. Ahmed, H. Farag","doi":"10.21608/vmjg.2021.196538","DOIUrl":"https://doi.org/10.21608/vmjg.2021.196538","url":null,"abstract":"The skin is the largest organ of the body that has many functions. Canine dermatology remain a challenging field that requires a variety of knowledge as dermatological problems are reported to be the most common health problem in small animal practice. Blood, skin and biopsy samples were collected from 50 dogs of different breeds and sexes. The present study is designed for identification of the different etiological agents causing skin diseases in dogs, evaluation of some hematological and biochemical parameters in diseased dogs and histopathological examination of selected cases using skin biopsy. These dogs were grouped into two groups, the first one was the control group (n=10) apparently healthy dogs and the second group (n=40) diseased dogs presented with different skin ailments where 12 out of 40 dogs were suffered from demodicosis, dermatophytosis (6 cases), pyoderma (8 cases) , mixed infection (10 cases) and canine atopic dermatitis (4 cases). Evaluation of the hematological parameters revealed presence of anemia and leukocytosis with apparent neutrophilia in dogs with pyoderma and eosinophilia in the other groups. Biochemical parameters lied within the reference range in all diseased groups. Staphylococcus spp. was the most common bacterial isolate in canine pyoderma. With respect to histopathological picture, results indicated presence of large focal area of epidermal liquefactive necrosis associated with dense neutrophils infiltration. Severe dermatitis, folliculitis, perifollicular and perivascular inflammatory cells infiltration, all these changes associated with cases of pyoderma. Demodicosis cases demonstrated as heavy mite infestation in different developmental stages in the stratum conium of the epidermis and in the follicles. Laminar orthokeratotic hyperkeratosis, vacuolated epidermal prickle cells, acanthosis and vaculation of keratinocytes of the infundibula. Dermatitis characterized by moderate to marked inflammatory infiltration. Necrosis of adnexa, folliculitis and perifollicultis were also recorded in demodecosis infected dogs. Fungal dermatosis showed pronounced laminar orthokeratotic hyperkeratosis and acanthosis. Vacuolar degeneration of keratinocytes and fungal Hyphae were seen in dermatophytosis infected dog. Ulcerative dermatitis in form of focal extensive area of epidermal necrosis and ulceration associated with inflammatory cells infiltration, acanthosis and spongiosis of prickle cell layer were noticed in mixed infection infected dogs. The most common infectious skin problems during our study were pyoderma, red mange and dermatophytosis. The predominant pathogen in pyoderma affected dogs was Staphylococcus spp. Histopathological examination was very useful in our study as they provide accurate diagnosis in a short period of time and help in clear visualization of the clinical picture.","PeriodicalId":202817,"journal":{"name":"Veterinary Medical Journal (Giza)","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115063000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Echocardiographic measurements of apparently Healthy Persian cats","authors":"Adel Kubesy, M. Ali, Ghada Ashraf","doi":"10.21608/vmjg.2021.192301","DOIUrl":"https://doi.org/10.21608/vmjg.2021.192301","url":null,"abstract":"Background:The objective of this study is to determine normal values for 2-Dimension (2D) and Motion-mode (M-mode) echocardiographic parameters in apparently healthy Persian cats. Apparently normal cats should be screened for hypertrophic cardiomyopathy. Aim: This study has two aims, first, to determine the normal M-mode and 2D echocardiographic measurements in Persian cats obtained from right lateral recumbent position and express the relation of these measurements with gender, age and body weight. Second, to study the prevalence of hypertrophic cardiomyopathy in Persian cats. Materials and Methods: Seventy eight (78) apparently healthy Persian cats, weighing between 2 and 5 kg, age range (2 to 14) years and of both sexes were enrolled in this study. Cats were subjected to M-mode echocardiography. Measurements from the right parasternal longitudinal axis left ventricular outflow tract view of the heart. Left ventricular internal dimension, interventricular septal thickness and left ventricular posterior wall thickness during diastole and systole, left atrial diameter, aortic root diameter, left ventricular systolic functional parameters, and indices were all measured. Left ventricular hypertrophy was identified when posterior wall during end of diastole ≥ 6 mm. So a separated group was made for Persian cats whose Posterior wall during diastole ≥ 6 mm to detect the apparently healthy Persian cats with Hypertrophic cardiomyopathy. Results: Gender showed statistically significant on heart rate (p=0.002). Age has statistical significant on left ventricle posterior wall during diastole. Body weight has statistical significance on left ventricle posterior wall during systole. Gender, age and weight have no effect on left ventricle internal diameter during systole and diastole, interventricular septum during systole and diastole, Left atrium and aorta. Hypertrophic cardiomyopathy is identified in 12 (15.3%) of the 78 examined cats. Conclusion: Gender has no effect on echocardiographic measurements except Heart rate is higher in females than in males, posterior wall during diastole increase by increasing age and posterior wall during systole increase by increasing body weight. Apparently normal cats should be screened for HCM.","PeriodicalId":202817,"journal":{"name":"Veterinary Medical Journal (Giza)","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126363721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Histological, and Histochemical Studies on the Tongue of Domestic Turkey (Meleagris Gallopavo)","authors":"Ebtihal M. M. Elleithy","doi":"10.21608/vmjg.2021.192319","DOIUrl":"https://doi.org/10.21608/vmjg.2021.192319","url":null,"abstract":"The purpose of this study was to investigate the micromorphological features of the tongue of domestic turkey (Meleagris Gallopavo). The tongue was studied by means of histological and histochemical methods to elucidate its micromorphological features. Six adult male domestic turkeys were used in this study. Samples from the tip, body and root portions of the tongue were fixed in 10% neutral buffered formalin. Stained sections with H&E revealed that the lingual mucosal lining is stratified squamous epithelium keratinized thicker in the dorsal surface than that of the ventral surface throughout the length of the tongue. The core of the tongue had a special lingual structure varied along the length of the tongue started at the apex as hyaline cartilage and changed in the body into fibrocartilage and continued caudally as spongy bone. By Alcian blue pH 2.5 the lingual salivary glands gave a strong reaction and appeared as clusters at the level of the body","PeriodicalId":202817,"journal":{"name":"Veterinary Medical Journal (Giza)","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117138783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Hassan, Wageih Armanious, Sabry Morgan, A. Samir, A. Orabi
{"title":"Overview on Listeria monocytogenes ecological niches, antibacterial susceptibility and virulence in bovine, caprine and ovine udder infection as a public health implication.","authors":"N. Hassan, Wageih Armanious, Sabry Morgan, A. Samir, A. Orabi","doi":"10.21608/vmjg.2021.192304","DOIUrl":"https://doi.org/10.21608/vmjg.2021.192304","url":null,"abstract":"A total of 410 milk samples were collected from different ruminant species as the following ; cows (n = 110), buffaloes (n = 87) ) , does (n=108) ) and ewes (n=105) from different Egyptian farms .The results revealed that prominence of L.monocytogenes in the examined milk samples are 1.72 % at apparently healthy cow , 2.77 % in subclinical mastitic cow and in apparently healthy goat is 1.38 % and this has a public health importance. The recovered isolates identified biochemically as L.monocytogenes confirmed phenotypically by positive hemolytic reaction and motility , followed by molecular confirmation using specific primer sequences of 16s rRNA at 1200 bp followed with sequencing and accession on genbank . Antibiogram of the isolated L. monocytogenes are demonstrated resistance to tetracyclines, lincomycin, streptomycin, cefotaxime, Trimethoprime sulfamethoxazole, florphenicols, and clindamycin, while adherence assay for test glass tube polystyrene microtiter plates conclude that L. monocytogenes was able to form a strong biofilm.","PeriodicalId":202817,"journal":{"name":"Veterinary Medical Journal (Giza)","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123779930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Raheel, A. Orabi, A. Erfan, Hassan Shaheen, S. Hassan
{"title":"Immune status of broiler chickens experimentally infected with avian E.coli supplemented with amino acid mixture rich in Glycine","authors":"I. Raheel, A. Orabi, A. Erfan, Hassan Shaheen, S. Hassan","doi":"10.21608/vmjg.2021.192316","DOIUrl":"https://doi.org/10.21608/vmjg.2021.192316","url":null,"abstract":"Meat producing broiler chickens may needs dietary amino acids of animal origin to obtain high growth, good health, muscles yield and healthy immune response. To investigate the effects of the amino acids mixture from animal origins rich in glycine on performance and immunity of broiler chickens an in vivo experiment was conducted at which T1 andT2 groups supplemented with amino acid mixture in every 10 days for successive 3 days, 8 hr. daily interval at dose 1 ml/L in drinking water and the second ( T2 group) was experimentally challenged with avian pathogenic E.coli (APEC) and the third group (T3) had no supplementation. The obtained results showed that amino acids mixture treated groups( T1 and T2) had a remarkable immune-modulation as noticed by significant elevation of phagocytic count and activities(lysozymes and nitric oxide production) ,expression of IL-4, IL-10 and interferon- gamma-genes using RT-PCR. Performance studies proved that there was an improvement of body weight gain and lowered mortality rate in amino acids treated group.","PeriodicalId":202817,"journal":{"name":"Veterinary Medical Journal (Giza)","volume":"95 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125178259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Isolation of Staphylococcus spp. and Pseudomonas spp from small animals' clinics: A potential hazard for nosocomial infections","authors":"Osamah M. Hassan, Mohamed Ali, Hassan El-agrab","doi":"10.21608/VMJG.2021.184887","DOIUrl":"https://doi.org/10.21608/VMJG.2021.184887","url":null,"abstract":"Veterinary clinic environments contain a variety of pathogens that can cause infection in animals. Pseudomonas aeruginosa is one of the most common Gram-negative nosocomial pathogens due to its versatility in nutrients and universal presence. Staphylococcus pseudintermedius and Staphylococcus aureus usually colonize the skin, mucous membranes, urogenital tract, and occasionally the alimentary tract in dogs and humans. This study was carried out on 250 samples collected from different clinics in Egypt for isolation of Staphylococcus and Pseudomonas spp. Among 250 samples, 125 samples were used for Staphylococcus isolation, revealing 77 samples had staphylococcus, with high count in floor and examination table respectively, while another 125 samples were used for Pseudomonas isolation, revealing 80 samples showed growth of Pseudomonas with high count in floor and examination table respectively. present especially in the examination table and floor. The improper cleaning program and disinfection lead to high results for isolated Staphylococcus and Pseudomonas species in the environmental surfaces in small animal's clinics","PeriodicalId":202817,"journal":{"name":"Veterinary Medical Journal (Giza)","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123748846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Farid, Shayma A. Hussein, Amr El-Karmouty, Yasser Wally
{"title":"Some anatomical and histological studies on the pharynx of the Goat (Capra-Hircus)","authors":"M. Farid, Shayma A. Hussein, Amr El-Karmouty, Yasser Wally","doi":"10.21608/vmjg.2019.156589","DOIUrl":"https://doi.org/10.21608/vmjg.2019.156589","url":null,"abstract":"","PeriodicalId":202817,"journal":{"name":"Veterinary Medical Journal (Giza)","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129616352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dina AbuElghait, K. Mohamed, E. Sheta, K. Abdel-moein, Naglaa A AbdElkader, Hala M. Zaher, A. Samir
{"title":"Detection of Helicobacter equorum in Equine in Egypt","authors":"Dina AbuElghait, K. Mohamed, E. Sheta, K. Abdel-moein, Naglaa A AbdElkader, Hala M. Zaher, A. Samir","doi":"10.21608/vmjg.2019.156590","DOIUrl":"https://doi.org/10.21608/vmjg.2019.156590","url":null,"abstract":"Helicobacter pylori, previously known as Campylobacter pylori, is a gram-negative, microaerophilic, spiral (helical), multi-flagellated bacterium usually found in the stomach, horses, calves, pigs, rabbits, and chickens were evaluated for Helicobacter presence, and the pathogenetic effect on their gastric mucosa. Therefore in our study, fifty gastric lavage and fecal samples were collected from fifty diseased horses and application of Lateral Flow and PCR assays for detection of Helicobacter species. Positive results confirmed only by PCR which revealed that twelve samples were positive among the examined samples (24%), sequencing of 16s rRNA gene amplicon shared 100% identity with H.equorum on GenBank, young foals show higher prevalence than adult horses. Occasionaly, H.equorum act as a potential zoonotic risk hazard thus, further studies are needed to investigate the zoonotic pathway to the human contacts.","PeriodicalId":202817,"journal":{"name":"Veterinary Medical Journal (Giza)","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127670788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dina Abo El-Soud, S. Ahmed, H. Salem, A. Tolba, A. Samir
{"title":"Evaluation of Bacillus subtilis spores probiotic as an antibiotic alternative to protect broiler chickens against pathogenic E.coli and Clostridum perfringens","authors":"Dina Abo El-Soud, S. Ahmed, H. Salem, A. Tolba, A. Samir","doi":"10.21608/vmjg.2019.156585","DOIUrl":"https://doi.org/10.21608/vmjg.2019.156585","url":null,"abstract":"","PeriodicalId":202817,"journal":{"name":"Veterinary Medical Journal (Giza)","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130056787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Abou Gazia, Hazem Sherif, Ahmed M. Elsify, H. Elbaz, M. Elkamshishi, R. Ghallab
{"title":"Seroprevalence of Camel Brucellosis in Egypt","authors":"K. Abou Gazia, Hazem Sherif, Ahmed M. Elsify, H. Elbaz, M. Elkamshishi, R. Ghallab","doi":"10.21608/vmjg.2019.156588","DOIUrl":"https://doi.org/10.21608/vmjg.2019.156588","url":null,"abstract":"","PeriodicalId":202817,"journal":{"name":"Veterinary Medical Journal (Giza)","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129072184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}