Plant Breeding最新文献

{"title":"Development and application of a dCAPS marker for the white immature fruit rind colour gene w in cucumber (Cucumis sativus L.)","authors":"Yuqing Yang, Yuan Zhou, Yachen Xiang, Yuqing Zhou, Haibing Cui, Hanqiang Liu, Yupeng Pan","doi":"10.1111/pbr.13204","DOIUrl":"https://doi.org/10.1111/pbr.13204","url":null,"abstract":"Immature fruit rind colour, a key factor influencing consumer choice, is an important external quality trait in cucumbers. The application of molecular marker‐assisted selection (MAS) can significantly improve the efficiency of the cucumber breeding process that focuses on fruit rind colours. In this study, we developed a dCAPS marker Csw_dCAPS co‐segregated with cucumber white immature fruit rind regulating gene <jats:italic>w</jats:italic> (<jats:italic>Csaprr2</jats:italic>) based on its 1‐bp ‘G/‐’ sequence insertion. Both validations of marker Csw_dCAPS among 59 cucumber commercial cultivars and 41 inbred lines showed that the Csw_dCAPS marker has 100% accuracy rates in identifying the green and white coloured immature fruit rinds controlling by alleles <jats:italic>W</jats:italic> and <jats:italic>w</jats:italic>. Application of Csw_dCAPS marker in cucumber lines XNS023‐1 and XNS023‐2 revealed that the white immature fruit rind in XNS023‐1 is also caused by the 1‐bp ‘G/‐’ insertion of gene <jats:italic>w</jats:italic>. We discussed the derivation of gene <jats:italic>w</jats:italic> in cucumber XNS023‐1 according to the genetic background differences between XNS023‐1 and XNS023‐2. Besides, this newly developed marker was also compared with the previously reported linkage marker SSR06791 to underscore the precision and reliability of Csw_dCAPS. Herein, we first reported and verified a dCAPS marker Csw_dCAPS that completely co‐segregated with the white immature fruit rind regulating gene <jats:italic>w</jats:italic>. We concluded that this high‐accuracy dCAPS marker can effectively facilitate the MAS breeding process of new cucumber cultivars characterized by white‐coloured immature fruits.","PeriodicalId":20228,"journal":{"name":"Plant Breeding","volume":"15 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142207100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maintenance Breeding in Pigeonpea [Cajanus cajan (L.) Millspaugh] — An Assessment and Advanced Strategies","authors":"Kul Bhushan Saxena, Rachit K Saxena, Vijay A Dalvi, Arbind K Choudhary, Rafat Sultana","doi":"10.1111/pbr.13209","DOIUrl":"https://doi.org/10.1111/pbr.13209","url":null,"abstract":"The sustainable maintenance breeding of pigeonpea germplasm, genetic stocks, released cultivars, and hybrid parents is a tough job. The prime reason for their genetic contamination is the insect‐aided cross‐pollination. Although various public and private seed agencies are involved in maintenance breeding to produce different grades of pigeonpea seeds, but the limitation of resources and low priority make it difficult to meet the national seed requirements. This manuscript, aside from reviewing the present pigeonpea maintenance breeding methods, their limitations, and accomplishments, also highlights some advanced breeding and genomics approaches to enhance the efficiency of maintenance breeding.","PeriodicalId":20228,"journal":{"name":"Plant Breeding","volume":"17 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142207102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding the genetic basis of ear‐related traits in maize (Zea mays L.) using linkage mapping, association mapping and genomic prediction","authors":"Liguo Chang, Kunhui He, Jianchao Liu","doi":"10.1111/pbr.13212","DOIUrl":"https://doi.org/10.1111/pbr.13212","url":null,"abstract":"As important yield components, the genetic analysis of ear‐related traits could provide a theoretical basis for maize breeding. Here, we reported the comprehensive genetic architecture of five ear‐related traits using 150 recombinant inbred lines (RIL) populations derived from the cross between Xu178 and K12. Besides, two sets of association populations were used to dissect genetic loci of five traits by genome‐wide association study (GWAS). A total of 32 QTLs of ear‐related traits were detected in the linkage mapping. Based on the mixed linear model (MLM), a total of 117 significant SNP markers of ear‐related traits were detected. Furthermore, a combined GWAS and linkage mapping analysis revealed 51 significant SNP markers fell within the confidence interval of QTLs. A total of seven co‐located significant SNP markers among different traits were found. Finally, six important candidate genes related to grain development were screened out. In addition, through haplotype analysis, two favourable haplotypes were found on chromosome 4, which could increase row number per ear (RNE), kernel number per row (KNR) and grain yield per plant (GYP) to a certain extent. Compared with the random model, the prediction accuracy of genomic prediction (GP) was improved in different degrees by considering the significant SNP markers as fixed effects. The stable genetic QTLs, candidate genes and favourable haplotypes found in this study are valuable resources, which will provide theoretical reference for high‐yield breeding of maize. Taken together, the research results also highlight the benefits of integrating GWAS with GP to further improve the accuracy of GP.","PeriodicalId":20228,"journal":{"name":"Plant Breeding","volume":"9 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141929803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome size and chromosome number variation in sugarcane (Saccharum spp.) families and phenotypically contrasting Saccharum genotypes","authors":"José M. García, Aldo R. Andrada, Luis E. Erazzú, Alberto Acevedo","doi":"10.1111/pbr.13203","DOIUrl":"https://doi.org/10.1111/pbr.13203","url":null,"abstract":"Modern sugarcane (<jats:italic>Saccharum</jats:italic> spp.) cultivars originated from an intricate hybridization process, resulting in complex polyploid and aneuploid genomes. Flow cytometry (FCM), which has emerged as a precise and fast method for research on genome size in plants, has potential to unveil key aspects of sugarcane cytogenetics. In this study, FCM probes were conducted to estimate 2C DNA content in 175 <jats:italic>Saccharum</jats:italic> genotypes embracing phenotypically contrasting canes (commercial cultivars, energy‐cane hybrids, and a <jats:styled-content style=\"fixed-case\"><jats:italic>Saccharum spontaneum</jats:italic></jats:styled-content> accession) and full‐sib sugarcane families. FCM‐mediated DNA content estimates exhibited high accuracy in predicting chromosome numbers, offering a time‐efficient alternative to classical cytogenetic procedure. Low‐magnitude correlations detected between DNA content and phenotypic traits suggest that indirect selection of agronomic traits using FCM‐mediated DNA content estimates may not be efficient. DNA content variation patterns in sugarcane families varied according to the crossing, and revealed transgressive variations associated with rampant aneuploidy. Altogether, the findings uncover that loss or gain of genetic material constitutes a source of high genetic variability that may substantially impact on crop breeding.","PeriodicalId":20228,"journal":{"name":"Plant Breeding","volume":"49 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141873169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flavour improvement in early generations of fresh market tomatoes (Solanum lycopersicum L.): II. Response to breeders' sensory and marker‐assisted selection","authors":"Julia Hagenguth, Larissa Kanski, Hannah Kahle, Heiko C. Becker, Bernd Horneburg","doi":"10.1111/pbr.13202","DOIUrl":"https://doi.org/10.1111/pbr.13202","url":null,"abstract":"Fresh market tomatoes are often characterized by poor flavour. Flavour is a quantitative trait difficult to assess and often neglected in breeding. Classical sensory methods are not suitable for the evaluation of early segregating generations; simple physicochemical measurements are not sufficient to predict perceived aroma. Alternative selection methods are needed, such as the recently introduced Breeders' Sensory Test or marker‐assisted selection (MAS). Two unrelated crosses were chosen to evaluate the efficiency of phenotypic selection with the Breeders' Sensory Test (breeders' sensory selection, BS) and MAS for five quantitative trait loci for sweetness, sourness and tomato aroma (mapped in ‘Resi’ × ‘Auriga’) in organic low‐input and hydroponic cultivation. Selection for sensory attributes reduced fruit weight, emphasizing the challenge of breeding flavourful, large‐fruited tomatoes. Both selection methods were more effective in ‘Roterno F<jats:sub>1</jats:sub>‘× ‘Black Cherry’ derived from more distant parents. A trend towards higher efficiency of MAS in early segregating generations was observed for sweetness and tomato aroma. However, a major advantage of BS is its universal applicability. To improve flavour, combining both methods is recommended.","PeriodicalId":20228,"journal":{"name":"Plant Breeding","volume":"23 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141869292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sources of resistance to witches' broom disease in cacao (Theobroma cacao L.): Progress update and perspectives","authors":"Jocilene dos Santos Pereira, Ariana Silva Santos, Edna Dora Martins Newman Luz, Ronan Xavier Corrêa","doi":"10.1111/pbr.13205","DOIUrl":"https://doi.org/10.1111/pbr.13205","url":null,"abstract":"Sources of resistance are an important control measure for witches' broom disease in cacao. Their identification through quantitative trait loci (QTLs) and genetic diversity analysis would assist breeding programs to develop new cultivars with improved resistance traits. We conducted a systematic review that compiles the main advances in the search for resistance to witches' broom disease. The systematic review on Scopus, Springer Link, PubMed, SciELO, and Web of Science databases followed the Preferred Reporting Items for Systematic Reviews and Meta‐Analysis (PRISMA) guidelines using the State of the Art through Systematic Review (StArt) software. We identified genotypes exhibiting the most significant resistance in phenotypic analysis, along with combinations of crosses that are promising for future studies and cultivar development. Data on genetic diversity indicate a wide possibility of benefits from heterozygosis. Genetic mapping and association studies revealed eight QTLs associated with resistance and several candidate genes with known and unknown biological functions. More comprehensive research of the various sources of resistance to witches' broom disease is crucial for the advance of cacao breeding programs.","PeriodicalId":20228,"journal":{"name":"Plant Breeding","volume":"34 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141780290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative transcriptome profiling to untie the drought tolerance molecular mechanism of backcross rice (Oryza sativa L.) inbred","authors":"Kari Baghyalakshmi, Selvaraj Ramchander, Nallathambi Jagadeeshselvam, Muthurajan Raveendran, Paramasiwam Jeyaprakash","doi":"10.1111/pbr.13208","DOIUrl":"https://doi.org/10.1111/pbr.13208","url":null,"abstract":"Rice production is severely hampered by drought stress, which causes enormous economic losses. The issue of global climate change is gaining importance, and hence development of rice genotypes tolerant to drought stress is becoming more critical. To address this issue, backcross inbred lines (BILs) developed were subjected to drought stress, and their molecular mechanism was studied. The drought‐tolerant parent Apo and drought‐susceptible, high‐yielding IR64 along with two BILs, namely, CB 229 (&lt;jats:italic&gt;qDTY&lt;/jats:italic&gt;&lt;jats:sub&gt;&lt;jats:italic&gt;2.2&lt;/jats:italic&gt;&lt;/jats:sub&gt; + &lt;jats:italic&gt;qDTY&lt;/jats:italic&gt;&lt;jats:sub&gt;&lt;jats:italic&gt;3.1&lt;/jats:italic&gt;&lt;/jats:sub&gt; + &lt;jats:italic&gt;qDTY&lt;/jats:italic&gt;&lt;jats:sub&gt;&lt;jats:italic&gt;8.1&lt;/jats:italic&gt;&lt;/jats:sub&gt;) and CB 193‐3 (&lt;jats:italic&gt;qDTY&lt;/jats:italic&gt;&lt;jats:sub&gt;&lt;jats:italic&gt;3.1&lt;/jats:italic&gt;&lt;/jats:sub&gt; + &lt;jats:italic&gt;qDTY&lt;/jats:italic&gt;&lt;jats:sub&gt;&lt;jats:italic&gt;8.1&lt;/jats:italic&gt;&lt;/jats:sub&gt;) were tested in a greenhouse for their response to drought. In this study, CB 229 showed better performance under water stress irrigated conditions; it was on par with IR64. Drought‐responsive transcriptome profiling was carried out in both the parents and the superior BIL CB 229 through the RNA‐Seq approach. About 3050 differentially expressed genes (DEGs) (2021 upregulated and 1029 downregulated) were detected in tolerant BIL CB 229 in drought stress. Most of the DEGs were involved in carbohydrate metabolism and the formation of cell walls, as well as genes associated with metabolite adaptability, ROS homeostasis and post‐transcriptional regulation. Genes such as chaperone protein, senescence‐induced receptor‐like serine, mannose‐6‐phosphate isomerase, aquaporin and heat shock proteins (LOC_Os02g26840, LOC_Os02g25720, LOC_Os07g35570, LOC_Os01g64970, etc.) were upregulated in the tolerant Apo and CB 229. It was observed that the BIL CB 229 yielded higher grains than both parents under moisture stress. Ninety‐four genes in the quantitative trait loci (QTLs) region were found to be differentially regulated in CB 229, Apo and IR64. Out of 94, nine genes co‐localized within the QTL &lt;jats:italic&gt;qDTY&lt;/jats:italic&gt;&lt;jats:sub&gt;&lt;jats:italic&gt;2.2&lt;/jats:italic&gt;&lt;/jats:sub&gt;, 12 genes within &lt;jats:italic&gt;qDTY&lt;/jats:italic&gt;&lt;jats:sub&gt;&lt;jats:italic&gt;3.1&lt;/jats:italic&gt;&lt;/jats:sub&gt; and four genes within &lt;jats:italic&gt;qDTY&lt;/jats:italic&gt;&lt;jats:sub&gt;&lt;jats:italic&gt;8.1&lt;/jats:italic&gt;&lt;/jats:sub&gt; were differentially upregulated in CB 229 and downregulated in the susceptible genotype. The study revealed that the QTLs &lt;jats:italic&gt;qDTY&lt;/jats:italic&gt;&lt;jats:sub&gt;&lt;jats:italic&gt;2.2&lt;/jats:italic&gt;&lt;/jats:sub&gt;, &lt;jats:italic&gt;qDTY&lt;/jats:italic&gt;&lt;jats:sub&gt;&lt;jats:italic&gt;3.1&lt;/jats:italic&gt;&lt;/jats:sub&gt; and &lt;jats:italic&gt;qDTY&lt;/jats:italic&gt;&lt;jats:sub&gt;&lt;jats:italic&gt;8.1&lt;/jats:italic&gt;&lt;/jats:sub&gt; are found to have complementary effects and offer enhanced levels of tolerance against drought due to complementation. Additionally, this analysis discovered new DEGs that may be involved in functions r","PeriodicalId":20228,"journal":{"name":"Plant Breeding","volume":"24 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141785765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of red–blue light ratio on the phenology and morphology of different lentil (Lens culinaris Medik) and chickpea (Cicer arietinum) genotypes under a simple and resource‐efficient in‐house speed breeding method based on the application of extended photoperiod","authors":"Mohammed Mitache, Abdelmonim Zeroual, Aziz Baidani, Bouchaib Bencharki, Omar Idrissi","doi":"10.1111/pbr.13206","DOIUrl":"https://doi.org/10.1111/pbr.13206","url":null,"abstract":"In the complex context of rising food demand, natural resources degradation and worsening climate changes impact, food legumes could play an important role in sustainable agriculture and food security. To overcome these challenges, enhanced genetic gain has become a necessity. Speed breeding‐based extended photoperiod is being used to reinforce conventional breeding methods and to speed up the development of adapted varieties. Speed breeding growth chambers and greenhouses' light quality in terms of red–blue ratio is among important factors that need to be optimized to enhance the efficiency of this technique to accelerate plant growth and development while limiting plant stress. We have compared the effect of different light ratios of 5:3 red–blue, 3:5 red–blue and 4:4 white–white on phenological and morphological characteristics of three lentil and chickpea genotypes grown in a speed breeding‐based extended photoperiod growth chamber with 22 h light/2 h dark. The 5:3 red–blue light ratio increased plant height and reduced flowering time for the three tested lentil varieties with 36, 41 and 40 days after sowing, respectively, as well as for the three tested chickpea varieties with 42, 38 and 24 days after sowing, respectively. While the 3:5 red–blue ratio increased leaves and secondary stem growth for lentil; leaves and secondary stem growth, green canopy cover and seedling vigour for chickpea. Less vegetative growth and later flowering were observed under white light. Higher red–blue light ratio of 5:3 accelerated lentil and chickpea vegetative growth and flowering, making it more optimal for speed breeding‐based extended photoperiod. It should be pointed out that the suggested home‐designed and built protocol is cost‐effective and easy to implement especially in small‐scale breeding programmes in developing countries.","PeriodicalId":20228,"journal":{"name":"Plant Breeding","volume":"184 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141742193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prospects for tritordeum (×Tritordeum martinii A. Pujadas, Nothosp. Nov.) cereal breeding: Key points for future challenges","authors":"Cristina Rodríguez‐Suárez, María Dolores Requena‐Ramírez, Elisabetta Mazzucotelli, Anna Maria Mastrangelo, Ilaria Marcotuli, Agata Gadaleta, Antonio Martín, Dámaso Hornero‐Méndez, Sergio G. Atienza","doi":"10.1111/pbr.13207","DOIUrl":"https://doi.org/10.1111/pbr.13207","url":null,"abstract":"Tritordeum is the new cereal derived from crosses between the wild barley <jats:styled-content style=\"fixed-case\"><jats:italic>Hordeum chilense</jats:italic></jats:styled-content> Roem. et Schultz. and either durum or bread wheat, resulting in hexaploid and octoploid tritordeums, respectively. The success of tritordeum as a crop depends on the effectiveness of its breeding programme. In this work, new advanced tritordeum lines are screened for grain carotenoid content and disease susceptibility to analyse the impact of the current breeding strategies and to identify their strengths and putative limitations for future challenges. We conclude that selection for grain carotenoid content, the main strength for the tritordeum commercialization, should be reinforced not only by using the diversity of <jats:styled-content style=\"fixed-case\"><jats:italic>H. chilense</jats:italic></jats:styled-content> but also incorporating beneficial alleles from durum wheat. Furthermore, genes for stem rust resistance from the A and B wheat genomes must be incorporated into tritordeum breeding programme. Finally, when selecting for threshability, tritordeums without chromosome substitutions should be preferentially selected using a marker‐assisted selection approach.","PeriodicalId":20228,"journal":{"name":"Plant Breeding","volume":"12 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141742195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating effectiveness of clonal plant selection of alfalfa (Medicago sativa L.) and sainfoin (Onobrychis viciifolia Scop.) in mixtures: Mean performance and stability in a multi‐environment trial","authors":"Surendra Bhattarai, Hu Wang, Hari P. Poudel, Bill Biligetu","doi":"10.1111/pbr.13194","DOIUrl":"https://doi.org/10.1111/pbr.13194","url":null,"abstract":"Alfalfa (<jats:styled-content style=\"fixed-case\"><jats:italic>Medicago sativa</jats:italic></jats:styled-content> L.) ‐ grass or alfalfa ‐ sainfoin (<jats:styled-content style=\"fixed-case\"><jats:italic>Onobrychis viciifolia</jats:italic></jats:styled-content> Scop.) mixtures are commonly used for pastures in western Canada because of their high forage quality and their low risk of causing frothy bloat in grazing animals. However, the proportion of these two legumes declines in mixed forage stands over time. The objective of this study was to evaluate the effectiveness of selecting superior genotypes from clonally propagated alfalfa or sainfoin under plant competition in different growth environments. For this study, around 100 genotypes of each legume were cloned and transplanted into meadow bromegrass (<jats:italic>Bromus riparius</jats:italic> Relm.) or alfalfa swards at Saskatoon, SK and Lethbridge, AB, Canada in 2017. Genotype‐environment (G x E) interactions of alfalfa and sainfoin genotypes were analysed by an additive main‐effects and multiplicative interaction (AMMI) model. Significant variations in plant height, spring vigour and total dry matter yield (TDM) were observed for both species. Among the measured traits, plant height was a more highly heritable trait (H<jats:sup>2</jats:sup> = .16 for alfalfa and H<jats:sup>2</jats:sup> = .18 for sainfoin), while TDM was the least heritable (H<jats:sup>2</jats:sup> = .08 for alfalfa and H<jats:sup>2</jats:sup> = .04 for sainfoin). In the AMMI Analysis of variance for TDM, the genotype explained &lt;12% of the variation for both species, suggesting a direct selection of yield would result in a low genetic gain. The biplot mean performance (Y) x weighted average of absolute scores from the singular value decomposition of the matrix of BLUPs stability index (WAASB) identified several promising genotypes with superior performance and stability across different environments that were selected for producing synthetic lines. However, the synthetic lines of both alfalfa and sainfoin did not consistently exhibit superior performance in mixtures compared to their respective check cultivars.","PeriodicalId":20228,"journal":{"name":"Plant Breeding","volume":"13 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141742198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}