Physiology and Pharmacology最新文献_第4页

Association of two specific haplotypes of serotonin transporter gene with fluvoxamine treatment outcomein Iranian patients with obsessive compulsive disorder 伊朗强迫症患者血清素转运基因的两种特定单倍型与氟伏沙明治疗结果的关系

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Physiology and Pharmacology Pub Date : 2021-12-01 DOI: 10.52547/phypha.25.4.3

Sareh Asadi, F. Rashidi, J. Shams, A. Ahmadiani

引用次数: 0

The effect of swimming exercise on thyroid function, spatial memory and anxiety in normal and propylthiouracil-induced hypothyroidism in Wistar rats 游泳运动对正常及丙硫脲嘧啶所致甲状腺功能减退大鼠甲状腺功能、空间记忆及焦虑的影响

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Physiology and Pharmacology Pub Date : 2021-09-01 DOI: 10.52547/ppj.25.3.231

Zulkhah Noor, Denny Agustiningsih, M. Soesatyo, S. Soejono

{"title":"The effect of swimming exercise on thyroid function, spatial memory and anxiety in normal and propylthiouracil-induced hypothyroidism in Wistar rats","authors":"Zulkhah Noor, Denny Agustiningsih, M. Soesatyo, S. Soejono","doi":"10.52547/ppj.25.3.231","DOIUrl":"https://doi.org/10.52547/ppj.25.3.231","url":null,"abstract":"Introduction: Swimming exercises improve various nerve growth factors and angiogenesis that encouraged the researchers to investigate the effect of swimming exercises on thyroid function (cyclic adenosine monophosphate [cAMP] and dual oxidase 2 [DUOX2] in free T4 [FT4] secretion), spatial memory, behavior and anxiety on normal Wistar pups and those given with propylthiouracil (PTU). Methods: The subjects of this research were normal Wistar pups and those given 25 ppm PTU from the 1st week until the 12th week of age. Swimming activity was started in 4-weekold pups after acclimatization for 1 week. Swimming exercises were conducted with a load of 1–2% body weight and 30min duration per day, 5 times a week for 8 weeks. The levels of FT4 serum, cAMP and DUOX2 of the thyroid gland homogenate were measured using enzyme-linked immunosorbent assay. The rats’ ability to maintain spatial memory was measured using the Morris water maze and anxiety using the open field maze. Results: The FT4 levels significantly decreased after the administration of 25 ppm PTU for 3 weeks and it was much more decreased after 12 weeks administration. The administration of 25 ppm PTU for 12 weeks reduced the cAMP levels, increased DUOX2 and reduced the spatial memory skills and exploration behavior of rats. Swimming exercise increased and normalized these parameters. Conclusion: Swimming exercises for 8 weeks improve thyroid function, learning ability and spatial memory of normal and PTU-induced hypothyroid Wistar rats.","PeriodicalId":20151,"journal":{"name":"Physiology and Pharmacology","volume":"13 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84326969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-dose fluoxetine improved long-term potentiation of the hippocampal dentate gyrus in male rats 大剂量氟西汀改善雄性大鼠海马齿状回的长期增强

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Physiology and Pharmacology Pub Date : 2021-09-01 DOI: 10.52547/ppj.25.3.223

S. Shahidi, Leila Rabiee, A. Komaki, Reihaneh Sadeghian

{"title":"High-dose fluoxetine improved long-term potentiation of the hippocampal dentate gyrus in male rats","authors":"S. Shahidi, Leila Rabiee, A. Komaki, Reihaneh Sadeghian","doi":"10.52547/ppj.25.3.223","DOIUrl":"https://doi.org/10.52547/ppj.25.3.223","url":null,"abstract":"Introduction: Paradoxical data have been reported regarding the effects of fluoxetine on different types of learning and memory. Hippocampus-dependent memory is mediated by long-term potentiation (LTP). Here, we evaluated the effects of acute administration of fluoxetine on LTP induction in the hippocampal dentate gyrus of intact rats. Methods: Eighteen rats were divided into three groups: the control group received saline 15min before high-frequency stimulation (HFS) and the fluoxetine groups were treated with fluoxetine (2 or 10mg/kg), 15min before HFS. The rats were anesthetized with urethane and put in a stereotaxic system for surgery, electrode implantation and field recording. After ensuring a steady-state baseline response, a single intraperitoneal injection of saline or fluoxetine (2 or 10mg/kg) was done. Next, population spike amplitude, excitatory postsynaptic potential (EPSP) slope, and paired-pulse stimuli (to determine recurrent inhibitory interneuron) were measured in the hippocampal dentate gyrus in three groups. Results: The results showed that population spike amplitude markedly increased in the fluoxetine (2 and 10mg/kg) group than in the saline group. Also, EPSP slope induction in the fluoxetine (10mg/kg) group showed an increase, 60min after HFS compared with the control group. Fluoxetine did not significantly affect recurrent inhibition. Conclusion: These results indicated that the acute administration of high-dose fluoxetine (10mg/kg) can induce LTP. Thus, fluoxetine can be considered as a memory enhancer in intact rats.","PeriodicalId":20151,"journal":{"name":"Physiology and Pharmacology","volume":"1 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81360764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The protective effects of apigenin on OLN-93 cellular model of cerebral stroke 芹菜素对脑卒中OLN-93细胞模型的保护作用

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Physiology and Pharmacology Pub Date : 2021-09-01 DOI: 10.52547/ppj.25.3.270

Samira Miraee, M. Farhadi, S. B. Jameie, Shabnam Najafie

{"title":"The protective effects of apigenin on OLN-93 cellular model of cerebral stroke","authors":"Samira Miraee, M. Farhadi, S. B. Jameie, Shabnam Najafie","doi":"10.52547/ppj.25.3.270","DOIUrl":"https://doi.org/10.52547/ppj.25.3.270","url":null,"abstract":"Introduction: Cerebral stroke is known to be the third most common cause of death in the world. To study pathophysiology and effects of the therapeutic agents on stroke, the cellular model of stroke recently was used more. ONL-93, oligodendrocyte like cell, is known as an appropriate model to study the role of glial cells in stroke. Apigenin is a flavonoid that has neuroprotective and neurogenic effects; therefore, the purpose of this study was to investigate the role of apigenin flavonoid on the OLN-93 cell line in terms of oxygen and glucose deprivation in the cellular stroke model. Methods: The cells were divided into experimental, negative and positive control groups. Then, MTT, reactive oxygen species (ROS), annexin and propidium iodide as well as Western blotting assays were performed to evaluate the viability and apoptosis. Results: The results showed that there was a significant increase in the number of live cells following administration of 1μM of apigenin in experimental groups and also, there was a significant difference in the number of live cells between two doses of 1μM and 0.75μM of the apigenin. The amount of ROS produced at a concentration of 1μM apigenin was a significant decrease compared to the positive control group and apoptotic cells also decreased significantly. The results for the expression of P53 protein showed a significant reduction in experimental groups. Conclusion: Based on our results, apigenin could have beneficial effects through the reduction of P53 and ROS production.","PeriodicalId":20151,"journal":{"name":"Physiology and Pharmacology","volume":"1 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84960758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The impact of mild and short-term social stress on urinary levels of T3 and iodine in students of elementary schools 轻度和短期社会压力对小学生尿T3和碘水平的影响

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Physiology and Pharmacology Pub Date : 2021-09-01 DOI: 10.52547/ppj.25.3.214

Sakineh Nouri Saeidlou, E. Saboory, L. Derafshpour, S. Masudi, Zafar Gholinejad, Yousef Rasmi

引用次数: 0

Antimicrobial potential of a gel containing hydrogen peroxide and hyaluronic acid 含有过氧化氢和透明质酸的凝胶的抗菌潜力

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Physiology and Pharmacology Pub Date : 2021-09-01 DOI: 10.52547/ppj.25.3.189

J. Tadakamadla, Elisa Boccalari, C. Maspero

引用次数: 0

Protective effects of date palm pollen extract on gentamicin-induced hepatotoxicity 枣花粉提取物对庆大霉素肝毒性的保护作用

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Physiology and Pharmacology Pub Date : 2021-09-01 DOI: 10.52547/ppj.25.3.251

Zeynab Mohamadi yarijani, S. Madani, S. Changizi-Ashtiyani, Houshang Najafi

引用次数: 0

Nicotine effects on enzymatic antioxidant defenses in human breast and ovarian cell lines 尼古丁对人乳腺和卵巢细胞系酶促抗氧化防御的影响

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Physiology and Pharmacology Pub Date : 2021-09-01 DOI: 10.52547/ppj.25.3.279

A. Yarahmadi, N. Mohammadi, F. Zal

{"title":"Nicotine effects on enzymatic antioxidant defenses in human breast and ovarian cell lines","authors":"A. Yarahmadi, N. Mohammadi, F. Zal","doi":"10.52547/ppj.25.3.279","DOIUrl":"https://doi.org/10.52547/ppj.25.3.279","url":null,"abstract":"Introduction: Nowadays, the prevalence of nicotine abuse among women has increased dramatically. In the current study, we aimed to investigate the effect of nicotine exposure on breast MCF-7 and ovarian OVCAR-3 cell lines for assessing the toxicity of nicotine in the cells of these organs. Methods: The MCF-7 and OVCAR-3 cells were treated with increasing nicotine concentrations ranging from 0 (control), 10-11, 10-8 and 10-6 M for 24h. Effect of nicotine treatments on major antioxidant enzymes catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), cellular levels of glutathione (GSH) and malondialdehyde (MDA) were monitored. Results: We showed that the CAT activity in MCF-7 cells increased only at 10-6 M dose of nicotine. The GPx and GR activity was decreased at 10-8 and 10-6 M of nicotine in MCF-7 cells, but in OVCAR-3 cells, this decrease was significant only at 10-6 M dose of nicotine. Reduced GSH decrease was statistically significant only at 10-8 and 10-6 M of nicotine in MCF-7 cells; otherwise, in OVCAR-3 cells, this decline was significant only at 10-6 M of nicotine. Nicotine at 10-8 and 10-6 M concentration caused a significant increase in MDA levels in MCF-7 cells. Conclusion: This study showed that breast MCF-7 cells are more vulnerable than ovarian OVCAR-3 cells against nicotine-induced oxidative toxicity.","PeriodicalId":20151,"journal":{"name":"Physiology and Pharmacology","volume":"58 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82891329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular mechanisms and signaling pathways involved in immunopathological events of COVID-19 COVID-19免疫病理事件的分子机制和信号通路

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Physiology and Pharmacology Pub Date : 2021-09-01 DOI: 10.52547/phypha.25.3.11

A. Peyvandi, S. Niknazar, F. Zare Mehrjerdi, Hojjat Allah Abbaszadeh, S. Khoshsirat, M. Peyvandi

{"title":"Molecular mechanisms and signaling pathways involved in immunopathological events of COVID-19","authors":"A. Peyvandi, S. Niknazar, F. Zare Mehrjerdi, Hojjat Allah Abbaszadeh, S. Khoshsirat, M. Peyvandi","doi":"10.52547/phypha.25.3.11","DOIUrl":"https://doi.org/10.52547/phypha.25.3.11","url":null,"abstract":"Introduction: COVID-19, a novel coronavirus that causes severe acute respiratory syndrome (SARS-CoV-2), is currently regarded as the most serious viral disease. During corona infection, viruses bind to host proteins and employ a variety of cellular pathways for their own purposes. Cell signaling is important for the regulation of cellular function. SARS-CoV-2 infection alters multiple signal transduction pathways that are critical for cell survival. The virus causes a severe and prolonged period of hypercytokinemia with misusing of these signaling cascades. Hyperactivation of the host immune system after infection with SARS-CoV-2 is the main cause of death in COVID-19 patients. Thus, to develop effective therapeutic approaches, it is necessary to first understand the problem and the underlying molecular pathways implicated in host immunological function/dysfunction. A number of intracellular signaling cascades have been implicated in infected cell pathways, including MAPK pathway, NF-Kappa B pathway, JAK-STAT signaling pathway, PI3K/AKT/mTOR pathway and TLRI signaling cascades. Here, we have presented the molecular insights on the potential mechanisms involved in immunopathological events of COVID-19.","PeriodicalId":20151,"journal":{"name":"Physiology and Pharmacology","volume":"66 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83149199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Antimicrobial potential of a gel containing hydrogen peroxide and hyaluronic acid (Letter) 含有过氧化氢和透明质酸的凝胶的抗菌潜力(字母)

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Physiology and Pharmacology Pub Date : 2021-09-01 DOI: 10.52547/PHYPHA.25.3.10

J. Tadakamadla, Elisa Boccalari, C. Maspero

引用次数: 0