High-dose fluoxetine improved long-term potentiation of the hippocampal dentate gyrus in male rats

IF 0.3 Q4 PHYSIOLOGY

Physiology and Pharmacology Pub Date : 2021-09-01 DOI:10.52547/ppj.25.3.223

S. Shahidi, Leila Rabiee, A. Komaki, Reihaneh Sadeghian

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引用次数: 2

Abstract

Introduction: Paradoxical data have been reported regarding the effects of fluoxetine on different types of learning and memory. Hippocampus-dependent memory is mediated by long-term potentiation (LTP). Here, we evaluated the effects of acute administration of fluoxetine on LTP induction in the hippocampal dentate gyrus of intact rats. Methods: Eighteen rats were divided into three groups: the control group received saline 15min before high-frequency stimulation (HFS) and the fluoxetine groups were treated with fluoxetine (2 or 10mg/kg), 15min before HFS. The rats were anesthetized with urethane and put in a stereotaxic system for surgery, electrode implantation and field recording. After ensuring a steady-state baseline response, a single intraperitoneal injection of saline or fluoxetine (2 or 10mg/kg) was done. Next, population spike amplitude, excitatory postsynaptic potential (EPSP) slope, and paired-pulse stimuli (to determine recurrent inhibitory interneuron) were measured in the hippocampal dentate gyrus in three groups. Results: The results showed that population spike amplitude markedly increased in the fluoxetine (2 and 10mg/kg) group than in the saline group. Also, EPSP slope induction in the fluoxetine (10mg/kg) group showed an increase, 60min after HFS compared with the control group. Fluoxetine did not significantly affect recurrent inhibition. Conclusion: These results indicated that the acute administration of high-dose fluoxetine (10mg/kg) can induce LTP. Thus, fluoxetine can be considered as a memory enhancer in intact rats.

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大剂量氟西汀改善雄性大鼠海马齿状回的长期增强

导言:关于氟西汀对不同类型学习和记忆的影响，有矛盾的数据报道。海马体依赖性记忆是由长期增强(LTP)介导的。在这里，我们评估了氟西汀急性给药对完整大鼠海马齿状回LTP诱导的影响。方法:将18只大鼠分为3组，对照组在高频刺激(HFS)前15min给予生理盐水，氟西汀组在高频刺激(HFS)前15min给予氟西汀(2或10mg/kg)。用氨基甲酸乙酯麻醉大鼠，置于立体定向系统中进行手术、电极植入和现场记录。在确保稳定的基线反应后，进行单次腹腔注射生理盐水或氟西汀(2或10mg/kg)。接下来，测量三组海马齿状回的群体峰值振幅、兴奋性突触后电位(EPSP)斜率和成对脉冲刺激(以确定复发抑制性中间神经元)。结果:氟西汀(2和10mg/kg)组的种群尖峰幅度明显高于生理盐水组。氟西汀(10mg/kg)组与对照组相比，HFS后60min EPSP斜率诱导增加。氟西汀对复发抑制无显著影响。结论:大剂量氟西汀(10mg/kg)急性给药可诱导LTP。因此，氟西汀可以被认为是完整大鼠的记忆增强剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Physiology and Pharmacology PHYSIOLOGY-

CiteScore

0.70

自引率

0.00%

发文量

期刊介绍： Physiology and Pharmacology is the official English publication of the Iranian Society of Physiology and Pharmacology. The journal publishes Review articles, Full-length original articles, Letter to editor and Short communications in physiology, pharmacology and related subjects. The aim of this journal is to provide a medium of scientific communication for investigators in the field of Physiology and Pharmacology. The editors will welcome original basic and applied research articles from Physiologists and Pharmacologists. Articles should be in English language. The papers submitted to this journal must not be Published or under consideration for publication elsewhere. Physiology and Pharmacology is an open access journal which means that all contents is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search or link to the full text of the articles in this journal without asking prior permission from the publisher or the author.