Plant Science Journal最新文献_第3页

Identification and evaluation of resistance to bacterial wilt in pepper 辣椒青枯病抗性鉴定与评价

Plant Science Journal Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.40378

F. Dang, Y. Lei, D. Guan, Zaiteng Wang, Shuilin He

引用次数: 2

Isolation and Screening of Microalgae with High-lipid Contents in Shanxi Province 山西高脂微藻的分离与筛选

Plant Science Journal Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.40415

Jianwei Ban, Jia Feng, S. Xie

{"title":"Isolation and Screening of Microalgae with High-lipid Contents in Shanxi Province","authors":"Jianwei Ban, Jia Feng, S. Xie","doi":"10.3724/SP.J.1142.2013.40415","DOIUrl":"https://doi.org/10.3724/SP.J.1142.2013.40415","url":null,"abstract":"This study attempted to choose potential oil-producing microalgae candidates across Shanxi province.Thirty-two strains of microalgae were tested.Among these microalgae,29 strains were isolated from water samples collected from four sites in the Fenghe River and the Salt Lakes Area in Yuncheng.Three strains of microalgae were bought from the Freshwater Algae Culture Collection of the Institute of Hydrobiology.Microalgae from the Shanxi province were isolated and purified by Micro-picking method and Spread-plate method.The biomass of 32 strains of microalgae varied between 48.9-422.2 mg/L,which were determined by dry weight.Chloroform-methanol extraction is a traditional total lipid measuring method and Nile red fluorescence is a new technique used for determining neutral lipids.In this study,Chloroform-methanol extraction and Nile red fluorescent staining were combined to determine lipid contents of the 32 strains of microalgae.The results showed that total lipid content of all strains were in the range of 5.4% DW-30.1% DW,and fluorescence value in unit volume were in the range of 4.1-181.5.High lipid productivity is a key selection criterion of species for biodiesel production.According to the lipid productivity of the microalgae,we screened three strains of microalgae that may be potential oil-producing candidates in Shanxi province.These three strains included NY017(Dunaliella salina),NY023(Nitzschia linearis)and NY025(Nitzschia palea).Their lipid productivities were 3.25 mg · L-1 · d-1,3.03 mg · L-1 · d and 2.11 mg · L-1 · d-1 respectively.","PeriodicalId":20134,"journal":{"name":"Plant Science Journal","volume":"31 1","pages":"415"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69788930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

A Revision of Some Species of the GenusPhyllostachys(Gramineae—Bambusoideae)(V) 毛竹属(禾本科-竹总科)若干种记述(V)

Plant Science Journal Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.60545

Lai Guang

引用次数: 0

Floral Vascular Comparative Anatomy ofStellaria mediaandMyosoton aquaticumand its Systematic Significance 中星与水草花维管比较解剖及其系统意义

Plant Science Journal Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.60525

Zu-Ren Li, N. Hu, Ji‐Gang Yang, B. Yang, Haimin Liao

引用次数: 0

Development of SSR Markers in Aquatic Plant Nymphoides peltata( Menyanthaceae ) Based on Information from Transcriptome Sequencing 基于转录组测序信息的水生植物绒球百合SSR标记的开发

Plant Science Journal Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.50485

Yangchen Yuan, Qing‐Feng Wang, Jin‐Ming Chen

{"title":"Development of SSR Markers in Aquatic Plant Nymphoides peltata( Menyanthaceae ) Based on Information from Transcriptome Sequencing","authors":"Yangchen Yuan, Qing‐Feng Wang, Jin‐Ming Chen","doi":"10.3724/SP.J.1142.2013.50485","DOIUrl":"https://doi.org/10.3724/SP.J.1142.2013.50485","url":null,"abstract":"Using the program MISA,a total of 12319EST-SSRs were sought out from 79536ESTs of aquatic plant Nymphoides peltata.Dinucleotide and trinucleotide SSRs were the two main motif types,accounting for 57.31% and 30.87%,respectively.The most abundant di-and tri-motifs were AG/CT(29.76%)and AAG/CTT(8.66%),respectively.We randomly selected 130primer pairs to evaluate their application and polymorphism across two populations of N.peltata.Seventyeight SSR markers were amplified successfully,yielding clear and discernible bands,among which37were found to be polymorphic and the polymorphic rate was 47.44%.Using the polymorphic SSR markers,a total of 114alleles were detected.The number of alleles per locus ranged from 2to 6(mean 3.08).Observed heterozygosity(Ho)and expected value(He)per locus varied from 0.229to 1.000and from 0.351to 0.756,respectively.Polymorphism information content(PIC)ranged from 0.286to 0.698,with an average of 0.495.The results indicated that the development of SSR molecular markers from the EST dataset generated by next generation sequencing in the aquatic plant N.peltata is valuable and effective.These newly generated SSR makers will provide new tools for studying genetic diversity and population genetic structure of N. peltata.","PeriodicalId":20134,"journal":{"name":"Plant Science Journal","volume":"31 1","pages":"485"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69789595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Functional Deletion Analysis ofFaChit1Promoter Region fromFestuca arundinacea 羊茅achit1启动子区功能缺失分析

Plant Science Journal Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.60562

Shidong Wang, Hao-Ru Wang, J. Wang

引用次数: 0

TOPSIS Based Comprehensive Evaluation of the Resistance in Wheat Germplasm to English Grain Aphid 基于TOPSIS的小麦种质抗蚜性综合评价

Plant Science Journal Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.30228

Fengqi Li, L. Kong, Yusheng Liu, He Wang, Jun-hua Peng

引用次数: 1

Development and Transfer Analysis of SSR in Dendrobium 石斛SSR的开发与转移分析

Plant Science Journal Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.50500

Qiu Daoshou, Xi-Long Zheng, Cai Shike, Jin-Rong Zheng, Lu Huanming, Zhang Lei, Deng Rui-yun, Wu Li, Liu Xiaojin

{"title":"Development and Transfer Analysis of SSR in Dendrobium","authors":"Qiu Daoshou, Xi-Long Zheng, Cai Shike, Jin-Rong Zheng, Lu Huanming, Zhang Lei, Deng Rui-yun, Wu Li, Liu Xiaojin","doi":"10.3724/SP.J.1142.2013.50500","DOIUrl":"https://doi.org/10.3724/SP.J.1142.2013.50500","url":null,"abstract":"To date,only 100SSR markers in Dendrobiumhave been developed,which are far from sufficient for research applications.To develop molecular markers,we mined SSR of Dendrobium from public nucleotide data through bioinformation methods.Some 1343Uni-DNA sequences were assembled from the 3599DNA sequences of Dendrobiumfrom GenBank.By scanning the Uni-DNA sequences,283SSRs were distributed in 205Uni-DNA sequences,with an average frequency of 1SSR per 2815bp.Sequence alignment indicated that 86of the 205SSR-DNA sequences had already been used to design primers.In this study,76primer pairs were designed from the remaining 119sequences for transferability analysis among 32 Dendrobium species.Results showed that 47primer pairs were amplified effectively with transfer rates ranging from 51.1%to 95.7%(average75.9%).Of which,46primer pairs were able to detect polymorphism among the Dendrobium species with 2-8alleles(average 4.0alleles).Ten pairs of polymorphic primers were selected to detect polymorphism in 60accessions of D.officinale,and 2-5alleles(average 3.4alleles)were found per SSR locus.Based on the SSR amplification pattern,the 60accessions of D.officinale were clustered into five clusters,and phenotypes were closer within clusters than between clusters.The sequencing of the amplified fragment of DM121revealed that allele variation within D.officinale was attributed mainly to the variation of SSR repeat numbers,whereas allele variations among Dendrobium species were also caused by a single base indel and substitution in the microsatellite flanking region.","PeriodicalId":20134,"journal":{"name":"Plant Science Journal","volume":"31 1","pages":"500"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69789268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research on Tissue Culture Technology of Racomitrium japonicum 日本血吸虫组织培养技术的研究

Plant Science Journal Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.60616

Mei-Juan Zhang, W. Sha

引用次数: 1

Expression and Localization of FtsZ from Spirulina platensis in Escherichia coli 螺旋藻FtsZ基因在大肠杆菌中的表达与定位

Plant Science Journal Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.20171

Juan Wu, Lu-Yang Zou, Qun Zeng, Hong Xu

{"title":"Expression and Localization of FtsZ from Spirulina platensis in Escherichia coli","authors":"Juan Wu, Lu-Yang Zou, Qun Zeng, Hong Xu","doi":"10.3724/SP.J.1142.2013.20171","DOIUrl":"https://doi.org/10.3724/SP.J.1142.2013.20171","url":null,"abstract":"Prokaryotic cytoskeletal protein FtsZ,a microtubule homolog,assembles into a compact circular structure at the mid-cell and plays an important role in cytokinesis.To explore the function of FtsZ in Spirulina platensis morphogenesis,we cloned the ftsZ gene from S.platensis and constructed its fusion tag of GFP expression plasmid pGFP-FtsZ.The recombined expression vector was transformed to Escherichia coli BL21.Western blot analysis showed that the GFP-FtsZ fusion gene was successfully expressed in the transformant.The transformed bacteria that expressed the GFP-FtsZ protein changed from normal short-rod shapes and formed long filaments.The length of the filamentation cells was proportional to the expression amount of FtsZ in cells.Regular-dot distribution of the GFP-FtsZ fusion protein in transformed bacteria was observed by fluorescent light microscopy.The data demonstrated that FtsZ was a highly conserved functional protein.The FtsZ of S.platensis assembled the complete cytokinesis apparatus and formed a Z-ring structure at the future division site to regulate cell division in E.coli.The overexpression of FtsZ blocked the normal cell cycle and led to cell filamentation.","PeriodicalId":20134,"journal":{"name":"Plant Science Journal","volume":"31 1","pages":"171"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69787730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0