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Effect of in vivo administration of parathyroid hormone on amino acid transport in membrane vesicles prepared from rabbit kidney tubules. 体内注射甲状旁腺激素对兔肾小管制备的膜囊中氨基酸转运的影响。
Physiological chemistry and physics Pub Date : 1982-01-01
P Bidot-Lopez, B Schinbeckler, B C O'Malley
{"title":"Effect of in vivo administration of parathyroid hormone on amino acid transport in membrane vesicles prepared from rabbit kidney tubules.","authors":"P Bidot-Lopez,&nbsp;B Schinbeckler,&nbsp;B C O'Malley","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It has been postulated that high concentrations of parathyroid hormone might increase transport of amino acids across basal lateral membrane into the cell and that the amino-aciduria seen in hyperparathyroidism occurs because of leakage across brush border membrane into the lumen. Accordingly, we separated plasma membranes from renal proximal convoluted tubules into basal lateral and brush border fractions, both of which form vesicles. We found that for the vesicles from rabbits pretreated with parathyroid hormone, 14C-L-alanine incorporation was indeed enhanced in the basal lateral fraction, but not in the brush border fraction. Nor was significant enhancement found for vesicles from rabbits pretreated with cycloheximide in addition to hormone. We conclude that L-alanine uptake is greatest in the basal lateral membrane fraction after parathyroid hormone treatment in vivo. In such case, the two membranes also differ from each other in endocrine response.</p>","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":"14 3","pages":"239-42"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18199377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preliminary studies of magnetic field facilitation of electric conduction in electrically switched "on" dye films that may be room-temperature superconductors. 在可能成为室温超导体的电开关染料薄膜中，磁场促进导电的初步研究。
Physiological chemistry and physics Pub Date : 1982-01-01
F W Cope
{"title":"Preliminary studies of magnetic field facilitation of electric conduction in electrically switched \"on\" dye films that may be room-temperature superconductors.","authors":"F W Cope","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Some organic dyes of a double diazo structure, pressurized into thin films, show trapping of magnetic flux at room temperature, suggestive of the behavior of type 2 superconductors. Some also show switching from low to high electrical conductivity at a voltage threshold, like amorphous inorganic semiconductors. An applied magnetic field facilitates switching, as well as current flow after switching, in a dye that traps magnetic flux but not in a dye of almost similar structure that does not trap magnetic flux. For best results, the magnetic field should be applied before switching and in a direction perpendicular to current flow. The magnetic field effects observed in the dye films at room temperature may be analogous to \"anomolous\" magnetic phenomena observed in metallic superconductors and/or in superconducting Josephson junctions at low temperatures. The implied possibility of superconductive mechanisms of biomagnetic effects, and apparent relation to the electrical switching seen in wet melanin, give the findings biological relevance.</p>","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":"14 5","pages":"423-30"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18200596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anion dependent swelling of crab nerve fibers during potassium- and veratridine depolarization. 钾和缬草碱去极化过程中蟹神经纤维的阴离子依赖性肿胀。
Physiological chemistry and physics Pub Date : 1982-01-01
K Iwasa
{"title":"Anion dependent swelling of crab nerve fibers during potassium- and veratridine depolarization.","authors":"K Iwasa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Swelling of crab claw nerve fibers, monitored as weight increase, takes place while the nerve fibers are immersed in a medium high in K+, Cs+ or Rb+ concentration, or in artificial seawater to which 0.2 mM veratridine is added. Similar swelling is observed in nerves in sodium salt solutions which also contain low concentrations of divalent cations. The swelling observed under these conditions is dependent on anions, giving a series (from efficient anion to less efficient ones): I- greater than SCN- greater than Br- greater than Cl- much greater than F- greater than glutamate, ethylsulfate. The presence of this series indicates that the nerve swelling observed is attributed to influx of external salt. Uptake measurements of radioactive anions and cations corroborate the presence of salt influx under these conditions. In analyzing the anion dependence of nerve swelling, two factors, membrane and cytoskeletal structure, are examined. It is shown that the cytoskeletal structure, which is sensitive to the lyotropic series of anions, plays an important role in this phenomenon.</p>","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":"14 6","pages":"503-12"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18202329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation of yeast nuclei. Evidence of chromatin-associated proteolytic activity. 酵母核的分离。染色质相关蛋白水解活性的证据。
Physiological chemistry and physics Pub Date : 1982-01-01
S Ruggieri, G Magni
{"title":"Isolation of yeast nuclei. Evidence of chromatin-associated proteolytic activity.","authors":"S Ruggieri,&nbsp;G Magni","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Purified yeast nuclei contain proteolytic activities which are associated with chromatin. pH optimum is in the range 8.0--8.5. Partial purification reveals the presence of three fractions corresponding to different molecular weights. Boiled chromatin supernatants are able to inhibit proteolytic activity. The inhibition effect of various compounds is also described. The purity of the chromatin preparation seems to rule out artifacts due to contamination.</p>","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":"14 4","pages":"315-22"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17816956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effects of some aldose sugars on the oxygen uptake in stimulated and non-stimulated isolated mouse pancreatic islets: configurational and conformational factors. 一些醛糖对受刺激和非受刺激小鼠离体胰岛摄氧量的影响:构型和构象因素。
Physiological chemistry and physics Pub Date : 1982-01-01
C Hellerstrom, N V Marsden
{"title":"The effects of some aldose sugars on the oxygen uptake in stimulated and non-stimulated isolated mouse pancreatic islets: configurational and conformational factors.","authors":"C Hellerstrom,&nbsp;N V Marsden","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":"14 6","pages":"495-502"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17817327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nonspecific and cooperative binding of lectins to microorganisms. 凝集素与微生物的非特异性和协同结合。
Physiological chemistry and physics Pub Date : 1982-01-01
L D Kahn
{"title":"Nonspecific and cooperative binding of lectins to microorganisms.","authors":"L D Kahn","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Binding of lectins to microbial cell walls was investigated by fluorimetric titration and Scatchard plot. Data were correlated with agglutinability. Concanavalin A and lectins of wheat germ, soybean, pea, lentil, and peanut were tested against Escherichia coli. Micrococcus luteus, Lactobacillus plantarum, and Bacillus subtilis. In cases where binding occurred, it was either nonspecific or positively cooperative. Agglutination was observed only in those combinations of lectin and microorganism that showed positive cooperative binding, suggesting a definite relation between binding and agglutination. Lectins binding to the same carbohydrate did not necessarily bind to the same microorganism, confirming the complexity of the lectin-receptor bond.</p>","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":"14 1","pages":"3-7"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17868678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Degradation of proteins synthesized in the presence of cycloheximide in normal and transformed fibroblasts. 正常和转化成纤维细胞中环己亚胺存在下合成蛋白质的降解。
Physiological chemistry and physics Pub Date : 1982-01-01
J Hernandez-Yago, E Knecht, A Martinez-Ramon, S Grisolia
{"title":"Degradation of proteins synthesized in the presence of cycloheximide in normal and transformed fibroblasts.","authors":"J Hernandez-Yago,&nbsp;E Knecht,&nbsp;A Martinez-Ramon,&nbsp;S Grisolia","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Monolayer cultures of 3T3 and WI-38 fibroblasts were pulse labeled with radioactive leucine in the presence of cycloheximide. The rate of protein degradation was measured and compared with that of SV-40 virus transformed cells. The results clearly show that normal and transformed cells have essentially identical rates of degradation of proteins synthesized in the presence of cycloheximide. These findings indicate that the lower rates of protein degradation observed in some transformed cells is not a general rule.</p>","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":"14 4","pages":"327-33"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17372542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PTH-induced amino acid transport in membrane vesicle preparations from rabbit kidney proximal convoluted tubule. pth诱导兔肾近曲小管膜泡制剂中氨基酸转运的研究。
Physiological chemistry and physics Pub Date : 1982-01-01
P Bidot-Lopez, B Schinbeckler, B C O'Malley
{"title":"PTH-induced amino acid transport in membrane vesicle preparations from rabbit kidney proximal convoluted tubule.","authors":"P Bidot-Lopez,&nbsp;B Schinbeckler,&nbsp;B C O'Malley","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":"14 2","pages":"169-76"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18199291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
General anesthetics directly inhibit electron mobility: dipole dispersion theory of anesthetic action. 全麻直接抑制电子迁移率:偶极色散理论的麻醉作用。
Physiological chemistry and physics Pub Date : 1982-01-01
S R Hameroff, R C Watt, J D Borel, G Carlson
{"title":"General anesthetics directly inhibit electron mobility: dipole dispersion theory of anesthetic action.","authors":"S R Hameroff,&nbsp;R C Watt,&nbsp;J D Borel,&nbsp;G Carlson","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A model system of gaseous electron mobility, excitation, and plasma activity was used to study direct effects of six gases, including four general anesthetics, in oxygen. Helium increased, and nitrogen had minimal effects on gaseous excitation. Nitrous oxide, as well as the potent anesthetics halothane, enflurane, and isoflurane, inhibited gaseous excitation, nitrous oxide having the weakest anesthetic effect. The data are compatible with the view that anesthetic inhibition is mediated by Van der Waals dipole dispersion interactions among anesthetic molecules (e.g., halogenated hydrocarbons) and electrons accelerated by the applied field. Dipole dispersion interactions may also mediate anesthetic effects on synaptic protein conformational control.</p>","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":"14 3","pages":"183-7"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18200703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Apparent similarity in protein compositions of maximally deviated cancer cells. 最大偏差癌细胞的蛋白质组成有明显的相似性。
Physiological chemistry and physics Pub Date : 1982-01-01
G N Ling, R C Murphy
{"title":"Apparent similarity in protein compositions of maximally deviated cancer cells.","authors":"G N Ling,&nbsp;R C Murphy","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":20124,"journal":{"name":"Physiological chemistry and physics","volume":"14 3","pages":"213"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18200707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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