A. Gentile, P. Nota, Gaia Urbinati, A. Frattarelli, C. Forni, E. Caboni, S. Lucioli
{"title":"Morpho-physiological effects of irrigation with saline water in ex vitro plants of Juglans regia ‘Sorrento’","authors":"A. Gentile, P. Nota, Gaia Urbinati, A. Frattarelli, C. Forni, E. Caboni, S. Lucioli","doi":"10.1080/11263504.2023.2234910","DOIUrl":"https://doi.org/10.1080/11263504.2023.2234910","url":null,"abstract":"Abstract Soil salinization is a global problem limiting growth and economical yield in fruit species including walnut. Knowledge of the response of varieties and cultivars to salinity can represent an important contribution to future walnut plantation in areas affected by soil salinization. Juglans regia ‘Sorrento’ is a commercially important Italian variety. The present work aimed to explore the morpho-physiological response to NaCl-induced stress in ‘Sorrento’ plants using clonally propagated ex vitro plantlets to reduce the impact of genetic variation. Ex vitro acclimatized plants were exposed to salinity conditions by irrigating pot soil with half-strength Hoagland solution containing 100 mM NaCl for 20 days. Salt-induced effects on plant growth, chlorophyll and carotenoid content, osmolality, membrane injury and on activities of antioxidant enzymes catalase and guaiacol peroxidase (G-POD) in leaves were evaluated. Morphological and growth parameters were not significantly altered by salt stress with respect to the control. Slight differences in leaf necrosis and absence of injury in the membranes, stable content of photosynthetic pigments, and an increase in G-POD activity were also found in salt-treated plants. These results collectively suggest that ‘Sorrento’ has potential for the cultivation in salinized environments.","PeriodicalId":20099,"journal":{"name":"Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology","volume":"26 1","pages":"984 - 991"},"PeriodicalIF":0.0,"publicationDate":"2023-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83098135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alessia Marino, F. Ori, G. Pacioni, M. Leonardi, M. Iotti
{"title":"Characterization of culturable truffle-inhabiting fungi isolated from Tuber melanosporum, T. aestivum and T. borchii ascomata","authors":"Alessia Marino, F. Ori, G. Pacioni, M. Leonardi, M. Iotti","doi":"10.1080/11263504.2023.2234904","DOIUrl":"https://doi.org/10.1080/11263504.2023.2234904","url":null,"abstract":"Abstract Truffles in the genus Tuber host and feed a wide community of organisms including insects, bacteria, and fungi. Among microorganisms, truffle-inhabiting fungi (TIF) have been poorly investigated and only a limited number of yeast and filamentous species have been identified. In this work we isolated and barcoded culturable TIF from 16 ascomata of T. melanosporum, T. aestivum and T. borchii of different provenances. Eighteen strains were identified a species level, four at genus level and one at order level. Penicillium was the most represented genus with five species and the only TIF genus isolated from T. melanosporum ascomata. Six TIF had colonies with a yeast morphology, including two species of Candida. Five species (Leohumicola sp., Knufia tsunedae, Hypocreales sp., Sagenomella cf. verticillata, Kondoa sp.) had rare ITS sequences compared to those deposited in GenBank. Except Peniophora cinerea, all TIF identified in this work have never been reported in Tuber ascomata until now. In-depth investigations on TIF and their host specificity are needed to evaluate the effects of these fungi on the truffle life cycle and the quality of ascomata.","PeriodicalId":20099,"journal":{"name":"Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology","volume":"16 1","pages":"978 - 983"},"PeriodicalIF":0.0,"publicationDate":"2023-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88491020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Salma Jlizi, Yassine Oulad El Majdoub, A. Zardi‐Bergaoui, F. Cacciola, Katia Arena, L. Mondello, A. Harrath, H. Ben Jannet
{"title":"HPLC-PDA/ESI-MS determination of the polyphenolic profile and evaluation of the cytotoxic effect of Tetraclinis articulata bark using in vitro and in silico methods","authors":"Salma Jlizi, Yassine Oulad El Majdoub, A. Zardi‐Bergaoui, F. Cacciola, Katia Arena, L. Mondello, A. Harrath, H. Ben Jannet","doi":"10.1080/11263504.2023.2231930","DOIUrl":"https://doi.org/10.1080/11263504.2023.2231930","url":null,"abstract":"Abstract The present research aimed to establish the polyphenolic profile and to appraise the cytotoxic activity first by in vitro analyses, followed by a confirmatory in silico study to establish a stronger biochemical rationale for the activity of the ethyl acetate extract obtained from the bark of Tetraclinis articulata. Total phenolics and flavonoids of the ethyl acetate extract were quantified (phenolics, 65.30 mg gallic acid equivalents/g extract; flavonoids, 28.90 mg quercetin equivalents/g extract). A total of 22 polyphenolic compounds were identified in the ethyl acetate extract by HPLC-PDA/ESI-MS analysis. Tetraclinis articulata extract exerted a significant cytotoxic effect against a HeLa cell line (IC50 = 07.02 ± 1.15 μg/mL). The in silico analysis was in agreement with the in vitro study in which major constituents of ethyl acetate extract showed low binding energy and a correct mode of interaction in the active pocket of the enzyme (PDB: 4I5I). These results might be due to the synergistic action of constituents present in ethyl acetate extract or acting separately. This is the first report on the polyphenolic composition and cytotoxic activity of the extract from the bark of T. articulata.","PeriodicalId":20099,"journal":{"name":"Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology","volume":"5 1","pages":"970 - 977"},"PeriodicalIF":0.0,"publicationDate":"2023-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85322986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Frigerio, G. Capotorti, E. D. Vico, Malika Ouled Larbi, F. Grassi, Carlo Blasi, M. Labra, W. Nissim
{"title":"Tree tracking: species selection and traceability for sustainable and biodiversity-friendly urban reforestation","authors":"J. Frigerio, G. Capotorti, E. D. Vico, Malika Ouled Larbi, F. Grassi, Carlo Blasi, M. Labra, W. Nissim","doi":"10.1080/11263504.2023.2234907","DOIUrl":"https://doi.org/10.1080/11263504.2023.2234907","url":null,"abstract":"Abstract The current climate change and increasing climate variability represent a serious global challenge that demands urgent responses. Among the multiple actions that should be taken, afforestation and reforestation are the most common strategies. The current paper aims to assess the potential of tree species to be used in urban forestry in the Po Plain ecoregional province (Northern Italy) using DNA barcoding approach for plant traceability and ecophysiological indexes (shade, drought and waterlogging) for plant selection. DNA barcoding markers results showed a 55.5% of success, but the power of discrimination increased to 94.5% when considering only the autochthonous ecoregional species pool. Concerning ecophysiological markers, Fagus sylvatica, Abies alba, Picea abies and Taxus baccata were very shade-tolerant. The best performing trees concerning drought tolerance were oak species (Quercus ilex, Q. pubescens and Q. cerris), pines (Pinus pinea, P. nigra and P. sylvestris) and Fraxinus ornus. Only a few tree species (Salix alba, Alnus glutinosa, Populus nigra and Prunus padus) showed high tolerance to waterlogging. The combination of both approaches makes it possible to select the most suitable species, based on eco-pedological and microclimatic conditions, and to trace their identity along the entire supply chain, from nursery to planting, for urban forestry. GRAPHICAL ABSTRACT","PeriodicalId":20099,"journal":{"name":"Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology","volume":"1 1","pages":"920 - 934"},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89652594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chayma Ikan, R. Ben-Laouane, Redouane Ouhaddou, C. Ghoulam, A. Meddich
{"title":"Co-inoculation of arbuscular mycorrhizal fungi and plant growth-promoting rhizobacteria can mitigate the effects of drought in wheat plants (Triticum durum)","authors":"Chayma Ikan, R. Ben-Laouane, Redouane Ouhaddou, C. Ghoulam, A. Meddich","doi":"10.1080/11263504.2023.2229856","DOIUrl":"https://doi.org/10.1080/11263504.2023.2229856","url":null,"abstract":"Abstract Drought stress (DS) is one of the severe abiotic stresses that cause reductions in agricultural production. This study was conducted to examine the efficiency of arbuscular mycorrhizal fungi (M: consortium) and plant growth-promoting rhizobacteria (R: Z2 and Z4 strains, B: CHIN14, AAP22, AAP2, and CHIN15 strains), individually or in combination, as an appropriate strategy to improve durum wheat productivity under two water regimes: well-watered (75% FC) and DS (25% FC). DS exhibition reduced biomass, stomatal conductance, stem water potential, and increased malondialdehyde and hydrogen peroxide content. Under DS, AMF or B promoted plant growth, such as shoot height by 49 and 17%, respectively; by activating the photosynthetic mechanism and increasing the synthesis of proline content by 369% in plants treated with AMF, and antioxidant enzyme activities, such as polyphenoloxydase (PPO), in leaves by 36% in plant inoculated with B. After the experiment, total organic matter and carbon of the soil were improved by single application of B by 99 and 317%, respectively. This finding implies that the combination of mycorrhiza and PGPR regulates the physiological and biochemical processes utilized by wheat to cope with drought and improves the comprehension of the soil-plant relationship.","PeriodicalId":20099,"journal":{"name":"Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology","volume":"10 1","pages":"907 - 919"},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82866471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Leandra Leto, S. L. Facchin, M. Micheli, B. Chiancone
{"title":"Tissue culture technology for sumac (Rhus coriaria) propagation","authors":"Leandra Leto, S. L. Facchin, M. Micheli, B. Chiancone","doi":"10.1080/11263504.2023.2229826","DOIUrl":"https://doi.org/10.1080/11263504.2023.2229826","url":null,"abstract":"Abstract The cultivation of Rhus coriaria, after a long period of abandonment, is spreading again, thanks to the increasing interest in natural products of which this plant is rich. Traditional sumac propagation, difficult and slow, does not guarantee to cover the plant request for new plantings; in vitro tissue culture can be a valid alternative to supply, in a short time and independently of the season, many plants. In this study, three in vitro tissue culture procedures and several auxin/cytokinin combinations were tested in order to identify a successful protocol for the mass propagation of sumac. A satisfying level of sprouts development was obtained from nodal explant culture and from encapsulated microcuttings, while a scarce response was given by in vitro-cultured petioles. None of the methods tested resulted in adventitious root formation; it will, therefore, be necessary to test further auxin/cytokinin combinations. To the best of our knowledge, this is the first study investigating in vitro sumac propagation, using the encapsulation and petiole culture methods. Results are encouraging and set the basis for further research to improve proliferation efficiency and to induce shoots to root. ABSTRACT","PeriodicalId":20099,"journal":{"name":"Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology","volume":"79 1","pages":"881 - 889"},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74275911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Serra, José C. Hernández, M. Alonso, M. B. Crespo
{"title":"Helianthemum bilyanense (Cistaceae), a new gypsum-halophytic specialist species from south-eastern Spain related to H. polygonoides","authors":"L. Serra, José C. Hernández, M. Alonso, M. B. Crespo","doi":"10.1080/11263504.2023.2231939","DOIUrl":"https://doi.org/10.1080/11263504.2023.2231939","url":null,"abstract":"Abstract A new species, Helianthemum bilyanense (sect. Helianthemum), is described from a single population in gypsum saltmarshes of inland Alicante province (Comunidad Valenciana), in the south-eastern part of the Iberian Peninsula. It is closely related to H. polygonoides, but several differences in its general habit, floral pieces and indumentum of stem, fruit and seed allow easy recognition. Data on morphology, ecology, distribution and conservation are reported for the new species, and its taxonomic affinities are discussed based on phylogenetic analyses of the internal transcribed spacer region of nuclear ribosomal DNA. Due to the scarce number of individuals, the reduced area of occupancy and the existence of serious threats on the only know population, H. bilyanense is suggested; (i) to be labelled as CR according to the IUCN red list categories and (ii) to be included in the Spanish and the Valencian catalogues of threatened plant species as ‘threatened with extinction’.","PeriodicalId":20099,"journal":{"name":"Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology","volume":"13 1","pages":"939 - 949"},"PeriodicalIF":0.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87241861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An integrative approach supports the taxonomic distinction of the Sardo-Corsican endemic Euphorbia semiperfoliata from the widespread E. amygdaloides (Euphorbiaceae)","authors":"Danka Caković, B. Frajman","doi":"10.1080/11263504.2023.2231926","DOIUrl":"https://doi.org/10.1080/11263504.2023.2231926","url":null,"abstract":"Abstract In the western Mediterranean, Corsica and Sardinia are characterised by high plant diversity and endemism, and exhibit a tight biogeographic connection. One of the endemic taxa thriving in forests, macchia, along riversides and in mountain grasslands of both islands is Euphorbia semiperfoliata. It was often considered a subspecies of the widespread European forest plant Euphorbia amygdaloides, or deemed closely related with the western Asian E. macroceras and E. oblongifolia. Here, we explored the evolutionary origin of E. semiperfoliata using nuclear ITS sequences that showed that E. semiperfoliata is divergent from E. amygdaloides and the other species of E. sect. Patellares, which indicates its old, likely Tertiary origin. In addition, its relative genome size is different from E. amygdaloides and other species and several morphological characters revealed by morphometric analyses differentiate E. semiperfoliata from E. amygdaloides. Finally, we provide a taxonomic treatment for E. semiperfoliata, including the typification and a morphological description.","PeriodicalId":20099,"journal":{"name":"Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology","volume":"10 1","pages":"958 - 969"},"PeriodicalIF":0.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77399987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Angelica remotiserrata (Apiaceae), a new species from Turkey based on morphological and molecular data","authors":"H. Tuncay, E. Akalın","doi":"10.1080/11263504.2023.2231947","DOIUrl":"https://doi.org/10.1080/11263504.2023.2231947","url":null,"abstract":"Abstract Angelica remotiserrata is a novel endemic species discovered in the Kastamonu provinces of northern Turkey. The new species is described by morphological, carpological, ecological and phylogenetic analysis. A. remotiserrata is morphologically distinct from Angelica sylvestris by the coarsely serrata leaf margins, the small and not swollen sheath, the larger fruit and the 12–14 dorsal vittae. A phylogenetic analysis based on the internal transcribed spacer (ITS) sequence of the ribosomal DNA was conducted to reveal the evolutionary relationships of the new species. Its relationships with related species and proposed conservation status were reviewed. The morphological, and molecular evidence clearly supports that A. remotiserrata is a new species.","PeriodicalId":20099,"journal":{"name":"Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology","volume":"37 1","pages":"950 - 957"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75475906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Quintino Giovanni Manni, R. Gennaio, G. Pellegrino
{"title":"An overview on the presence of Iris unguicularis (Iridaceae) in Salento (Apulia, southern Italy) and a new record of Iris unguicularis subsp. carica for the Italian vascular flora","authors":"Quintino Giovanni Manni, R. Gennaio, G. Pellegrino","doi":"10.1080/11263504.2023.2229849","DOIUrl":"https://doi.org/10.1080/11263504.2023.2229849","url":null,"abstract":"Abstract Iris unguicularis sensu lato is an endemic species to the Mediterranean region. In Italy, the species is alien and recorded in Liguria, Tuscany, Abruzzo, and Apulia administrative regions. In the latter region, the species was recorded in Salento, Province of Lecce (Southern Apulia), in three main stations: Li Fani, in the administrative territory of the municipality of Salve, Serra del Rio, Castiglione d’Otranto (Andrano) in the residual Mediterranean scrub areas, along with the new station of Serra of Masseria Grande, Spongano, and on a site near Surano. Morphological analyses and genetic investigations made it possible to establish how the specimens from Li Fani (Salve) belong to I. unguicularis subsp. unguicularis, a species of allochthonous origin, while the plants of Castiglione d’Otranto and Spongano represent a taxonomically distinct group, identifiable as I. unguicularis subsp. carica. The presence of this subspecies in Salento redefines the western boundaries of the area known to date, constituting a novelty for the Apulian and Italian flora.","PeriodicalId":20099,"journal":{"name":"Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology","volume":"50 1","pages":"890 - 898"},"PeriodicalIF":0.0,"publicationDate":"2023-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79788794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}