Xinran Gao, Kaiqiang Hao, Zhichao Du, Sijia Zhang, Zhiping Wang, M. An, Zihao Xia, Yuanhua Wu
{"title":"Identification of ceRNA-vsiRNA-mRNA network for exploring the mechanism underlying pathogenesis of sugarcane mosaic virus in resistant and susceptible maize inbred lines","authors":"Xinran Gao, Kaiqiang Hao, Zhichao Du, Sijia Zhang, Zhiping Wang, M. An, Zihao Xia, Yuanhua Wu","doi":"10.1186/s42483-023-00216-7","DOIUrl":"https://doi.org/10.1186/s42483-023-00216-7","url":null,"abstract":"","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"7 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138971867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Can Zhang, Shanshan Chen, Fan Zhang, Yuxin Zheng, Yuke Wang, Xili Liu
{"title":"The La-related protein PsLARP4_5 is crucial for zoospore production and pathogenicity in Phytophthora sojae","authors":"Can Zhang, Shanshan Chen, Fan Zhang, Yuxin Zheng, Yuke Wang, Xili Liu","doi":"10.1186/s42483-023-00210-z","DOIUrl":"https://doi.org/10.1186/s42483-023-00210-z","url":null,"abstract":"","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":" 27","pages":"1-10"},"PeriodicalIF":3.4,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139206933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yaqin Wang, Tao Hu, Hui Liu, Chenlu Su, Li Xie, Zhanqi Wang, Xueping Zhou
{"title":"Development and application of a self-assembling split-fluorescent protein toolkit to monitor geminiviral movement and infection in plant","authors":"Yaqin Wang, Tao Hu, Hui Liu, Chenlu Su, Li Xie, Zhanqi Wang, Xueping Zhou","doi":"10.1186/s42483-023-00209-6","DOIUrl":"https://doi.org/10.1186/s42483-023-00209-6","url":null,"abstract":"","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"27 1","pages":"1-10"},"PeriodicalIF":3.4,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139245411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The sucrose non-fermenting-1 kinase Snf1 is involved in fludioxonil resistance via interacting with the high osmolarity glycerol MAPK kinase Hog1 in Fusarium","authors":"Jing Wang, Ziyue Wen, Yun Chen, Zhonghua Ma","doi":"10.1186/s42483-023-00208-7","DOIUrl":"https://doi.org/10.1186/s42483-023-00208-7","url":null,"abstract":"","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"1 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139269398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Global phylodynamics of two relevant aphid-transmitted viruses in cucurbit crops: cucurbit aphid-borne yellows virus and watermelon mosaic virus","authors":"M. P. Rabadán, P. Gómez","doi":"10.1186/s42483-023-00207-8","DOIUrl":"https://doi.org/10.1186/s42483-023-00207-8","url":null,"abstract":"Abstract Cucurbit aphid-borne yellows virus (CABYV) and watermelon mosaic virus (WMV) are major plant pathogens that cause severe epidemics in cucurbit crops. While there has been an increasing interest in molecular epidemiological studies on both viruses at regional scales, their phylodynamic analysis by using the temporal data at global scale remains unexplored. In this study, we implemented the Nextstrain phylodynamic approach to comprehensively examine the coat protein gene and full-length genome sequences of the CABYV and WMV worldwide populations. Our analyses reconstructed a robust phylogeny of CABYV and confirmed the occurrence of isolates grouped into three clusters (Asian, Mediterranean, and Recombinant). Nextstrain analysis suggested that CABYV epidemics likely originated in Southeast Asia in fourteenth century, while the Mediterranean population emerged in Spain in seventeenth century. We also found a high divergence between Asian and Mediterranean isolates, with low genetic diversity and scarce evidence of selection, as reflected by the lack of gene flow. Moreover, the hierarchical analysis of molecular variance revealed a significant differentiation between CABYV populations grouped by geographical location and plant host. Additionally, the global phylogenetic reconstruction of the WMV population confirmed a clear differentiation among isolates, which grouped into two clusters (classical and emergent), and Nextstrain analysis suggested that WMV epidemics were most probably originated in USA during the sixteenth century. The initial WMV population diverged in the eighteenth century, with the origin of the emergent population in the nineteenth century. Our analysis confirmed that WMV population has a polyphyletic origin, defining an extensive genetic diversity. Overall, this work provides insights into the CABYV and WMV origin and evolutionary dynamics, gaining an understanding of the global spread of both viral diseases. Additionally, the integration of all spatio-temporal CABYV and WMV data, along with their genome sequence data by open access ( https://github.com/PiR92 ) and the Nextstrain approach, provides a valuable tool for compiling and sharing current knowledge of these viral diseases in cucurbit crops.","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"81 13","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134900999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ya-Zhou Zhang, Zhuo Li, Jie Man, Dan Xu, Lan Wen, Chen Yang, Qiang Xu, Qian-Tao Jiang, Guo-Yue Chen, Mei Deng, Yun-Liang Peng, Kai Zhong, Peng-Fei Qi, Yu-Ming Wei
{"title":"Genetic diversity of field Fusarium asiaticum and Fusarium graminearum isolates increases the risk of fungicide resistance","authors":"Ya-Zhou Zhang, Zhuo Li, Jie Man, Dan Xu, Lan Wen, Chen Yang, Qiang Xu, Qian-Tao Jiang, Guo-Yue Chen, Mei Deng, Yun-Liang Peng, Kai Zhong, Peng-Fei Qi, Yu-Ming Wei","doi":"10.1186/s42483-023-00206-9","DOIUrl":"https://doi.org/10.1186/s42483-023-00206-9","url":null,"abstract":"Abstract Fusarium head blight (FHB) caused by Fusarium species, seriously threatens the safety of wheat ( Triticum aestivum ) production. Resistant cultivars and fungicides are frequently used to control these FHB pathogens. However, Fusarium species have been adapting the current FHB control approaches in a manner that raises concern for future FHB control strategies, which could lead to a greater risk of FHB outbreaks. In this study, a total of 521 strains of Fusarium were isolated from Sichuan province of China, to investigate the diversity of Fusarium species and the genes associated with their adaptation. Seven species were identified based on molecular markers and morphological analysis. The virulence assays showed that Fusarium asiaticum (Fa) and Fusarium graminearum (Fg) were the two major causal agents of FHB, with high virulence and more frequent isolates. Fungicide resistance analysis showed that four isolates had developed the resistance to carbendazim, and four isolates had developed the resistance to tebuconazole. Of note, two point-mutation variants (F200Y and E198Q) occurred in the β2-tubulin gene, leading to the carbendazim resistance. The landscape of genomic diversity was analyzed through whole-genome sequencing, revealing a total of 182,811 and 430,733 variants (including: single nucleotide polymorphisms, SNP, insertion and deletion, Indel, and structure variation, SV) among the Fa and Fg isolates, respectively. In addition, potential alterations in gene function (15.22%) were predicted among Fg variants. These alterations offer potential helps for the Fusarium species to adapt to various managements of FHB, which may increase risks in developing fungicide-resistant isolates. However, these annotated genetic variants are valuable resources for further genetic and genomic studies, as well as potential markers to assist disease risk assessment.","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"49 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135432492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}