Phytopathology Research最新文献_第2页

Identification and genomic analyses of a novel actinobacterium Streptomyces shaowuensis sp. nov. with biocontrol potential for rice bacterial blight 具有水稻白叶枯病防治潜力的新型放线菌邵武链霉菌的鉴定与基因组分析

2区农林科学

Phytopathology Research Pub Date : 2025-03-20 DOI: 10.1186/s42483-025-00313-9

Ya‐Wen He, Jodi Woan‐Fei Law, Sepideh Mazhari Azad, Wenhua Hu, Kai Song, Kah Ooi Chua, Lian Jiang, Yu Jin, Learn−Han Lee, Lian Zhou

{"title":"Identification and genomic analyses of a novel actinobacterium Streptomyces shaowuensis sp. nov. with biocontrol potential for rice bacterial blight","authors":"Ya‐Wen He, Jodi Woan‐Fei Law, Sepideh Mazhari Azad, Wenhua Hu, Kai Song, Kah Ooi Chua, Lian Jiang, Yu Jin, Learn−Han Lee, Lian Zhou","doi":"10.1186/s42483-025-00313-9","DOIUrl":"https://doi.org/10.1186/s42483-025-00313-9","url":null,"abstract":"Abstract Bacterial leaf blight (BLB), caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most severe bacterial diseases in rice. The current BLB-prevention strategy depends on chemical antimicrobials. The biological control methods have gained considerable attention. Among them, Streptomyces are particularly promising candidates due to their ability to produce diverse natural antimicrobial and plant-growth-promoting metabolites. In this study, we isolated a Streptomyces strain HSW2009 from the rice rhizosphere. This strain displayed significant antagonistic activity against Xoo. The antagonistic metabolite was extracted and purified from the HSW2009 culture. High-performance liquid chromatography-mass chromatography and nuclear magnetic resonance analyses revealed that the active compound is Piericidin A1, a member of the piericidin family metabolites containing a 4-pyridinol core linked with a methylated polyketide side chain. Piericidin A1 was shown to protect rice from Xoo infection in the microclimate chamber. The strain HSW2009 produced pale yellow aerial mycelia on the agar plate of the International Streptomyces Project-2 medium. Its cellular morphology conformed to that typically observed in the genus Streptomyces . Phylogenetic analysis of 16S rRNA gene sequences showed that HSW2009 was closely related to Streptomyces zagrosensis , S. youssoufiensis , and S. varsoviensis . HSW2009 displayed a unique DNA profile in BOX-PCR fingerprinting analysis and had a genome size of 8,806,972 bp, with a 72.83% G + C content. Average nucleotide identity analysis and digital DNA–DNA hybridization using the Type Strain Genome Server supported HSW2009 as a novel Streptomyces sp. It was therefore proposed as Streptomyces shaowuensis sp. nov., type strain HSW2009.","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://phytopatholres.biomedcentral.com/counter/pdf/10.1186/s42483-025-00313-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147920202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Volatile organic compounds of hoary stock are responsible for suppressing downy mildew of grape in an intercropping system 间作葡萄霜霉病发生时，白垩原材挥发性有机化合物具有抑制霜霉病的作用

2区农林科学

Phytopathology Research Pub Date : 2025-01-14 DOI: 10.1186/s42483-024-00291-4

Weiping Deng, Fei Du, Rongliang Yang, Hanyang Yao, Min Yang, Xinyue Mei, Chen Ye, Su Li, Yixiang Liu, Huichuan Huang, Shusheng Zhu

{"title":"Volatile organic compounds of hoary stock are responsible for suppressing downy mildew of grape in an intercropping system","authors":"Weiping Deng, Fei Du, Rongliang Yang, Hanyang Yao, Min Yang, Xinyue Mei, Chen Ye, Su Li, Yixiang Liu, Huichuan Huang, Shusheng Zhu","doi":"10.1186/s42483-024-00291-4","DOIUrl":"https://doi.org/10.1186/s42483-024-00291-4","url":null,"abstract":"Abstract Grape downy mildew ( Plasmopara viticola ) is an air-borne disease and difficult to control. It has been observed that intercropping grapevines ( Vitis vinifera ) with aromatic plants can effectively suppress the airborne disease and volatile organic compounds (VOCs) from the aromatic plants are believed to have antimicrobial activities. In this study, a two-year field trial was established by intercropping grapevine and hoary stock ( Matthiola incana ) to evaluate the control of grape downy mildew. The field results showed that intercropping effectively suppressed grape downy mildew, particularly during the blooming stage of hoary stock. VOCs from hoary stock plants exhibited a dosage-dependent antimicrobial activity against grape downy mildew. To examine the role of VOCs, hoary stock plants were grown in an enclosed chamber, and VOCs were collected at the time points before and post blooming. The collected VOCs were subjected to gas chromatography-mass spectrometry (GC–MS) analysis and in vitro bioassays. Twenty-four VOCs found from pre-blooming and 36 VOCs from post-blooming hoary stock plants were identified. Seventeen VOCs demonstrated consistent inhibitory activities against P. viticola , including seven terpenoids, five benzenoids, and five aliphatics. Among the 17 VOCs, five were unique to post-blooming hoary stock, while 12 were common to both pre- and post-blooming hoary stock. The antimicrobial VOCs offers a potential eco-friendly alternative to managing downy mildew.","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://phytopatholres.biomedcentral.com/counter/pdf/10.1186/s42483-024-00291-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147882598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Identification and validation of a major QTL on chromosome 2A for wheat-Parastagonospora nodorum interactions. 小麦与芽孢副盘孢相互作用2A染色体上一个主要QTL的鉴定与验证。

IF 3.5 2区农林科学

Phytopathology Research Pub Date : 2025-01-01 Epub Date: 2025-09-24 DOI: 10.1186/s42483-025-00371-z

Cong Li, Xinyao He, Jian Ma, Pawan K Singh

{"title":"Identification and validation of a major QTL on chromosome 2A for wheat-Parastagonospora nodorum interactions.","authors":"Cong Li, Xinyao He, Jian Ma, Pawan K Singh","doi":"10.1186/s42483-025-00371-z","DOIUrl":"10.1186/s42483-025-00371-z","url":null,"abstract":"Wheat (Triticum aestivum L.) is frequently affected by Septoria nodorum blotch (SNB), a fungal disease that significantly reduces wheat yields. In this study, two recombinant inbred line (RIL) populations, developed from crosses involving two elite CIMMYT breeding lines (WUYA and KATH) and a common susceptible male parent (CIANO T79), were used to detect quantitative trait loci (QTL) associated with SNB resistance. High-density genetic maps were constructed for these RIL populations by incorporating presence/absence variation (PAV) markers using the DArTseq genotyping platform. Three major and stable QTL linked to SNB resistance were identified on chromosomes 2A, 4B, and 5B. Among these, QSnb.cim-2A accounted for 22.16%-28.74% and 17.62%-19.71% of the phenotypic variation in the WUYA/CIANO T79 and KATH/CIANO T79 populations, respectively, and it was also validated in the CASCABEL/CIANO T79 RIL population. The remaining two QTL, QSnb.cim-4B and QSnb.cim-5B, were found to be associated with Rht-B1b and tsn1, respectively. The combined effect of these three QTL significantly improved SNB resistance while also reducing plant height, indicating their promising utilization in wheat breeding programs.Supplementary information: The online version contains supplementary material available at 10.1186/s42483-025-00371-z.","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"7 1","pages":"82"},"PeriodicalIF":3.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457223/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145150484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryphonectria hypovirus 1 infection suppresses the pathogenicity but increases the mycotoxin deoxynivalenol production of Fusarium graminearum 低毒1型镰刀菌感染抑制了禾谷镰刀菌的致病性，但增加了真菌毒素脱氧雪腐镰刀菌醇的产生

2区农林科学

Phytopathology Research Pub Date : 2024-09-27 DOI: 10.1186/s42483-024-00272-7

Ruiling Bian, Huan Ren, Mengge Jiang, Zhihao Zhang, Shiyu Zhai, Tianxing Pang, Ida Bagus Andika, Liying Sun

{"title":"Cryphonectria hypovirus 1 infection suppresses the pathogenicity but increases the mycotoxin deoxynivalenol production of Fusarium graminearum","authors":"Ruiling Bian, Huan Ren, Mengge Jiang, Zhihao Zhang, Shiyu Zhai, Tianxing Pang, Ida Bagus Andika, Liying Sun","doi":"10.1186/s42483-024-00272-7","DOIUrl":"https://doi.org/10.1186/s42483-024-00272-7","url":null,"abstract":"Abstract Fusarium graminearum is a prominent pathogen responsible for causing head blight disease in small grain cereals, leading to substantial agricultural damage. A recent study revealed the infectivity of a hypovirus, Cryphonectria hypovirus 1 (CHV1), in F. graminearum . However, the effects of CHV1 on the pathogenicity and development of the fungus remain largely unexplored. In this study, we investigated the effects of CHV1 infection on F. graminearum and made several interesting discoveries. First, we observed that CHV1 infection attenuated the pathogenicity of F. graminearum and reduced its conidiation. CHV1 efficiently spread through various transmission modes, such as hyphal fusion and spores, including conidiospores and ascospores. However, the accumulation of the virus was reduced following transmission through spores. Interestingly, despite causing hypovirulence, CHV1 infection enhanced the production of deoxynivalenol (DON), a mycotoxin known to play a role in F. graminearum ’s virulence and induction of DON production by a DON-inducing medium elevated CHV1 accumulation. The transcriptional expression of key regulatory genes involved in DON biosynthesis or toxisome formation was upregulated in response to CHV1 infection. Furthermore, our study revealed that CHV1 infection suppressed the formation of lipid droplets. Overall, our study provides insights of how the infection of a hypovirulence-inducing mycovirus affects the physiological and metabolic processes in F. graminearum .","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s42483-024-00272-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147905039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Biosynthesis of cupric oxide nanoparticles: its antiviral activities against TMV by directly destroying virion and inducing plant resistance 氧化铜纳米颗粒的生物合成：通过直接破坏病毒粒子和诱导植物抗性来抗病毒TMV

2区农林科学

Phytopathology Research Pub Date : 2024-06-25 DOI: 10.1186/s42483-024-00250-z

Shuaikang Liu, Weiqiang Tian, Zhongwei Liu, Xuefeng Wei, Kai Yuan, Wei Du, S.H. Chen, Shuhan Chen, Dong Zhou, Lin Cai

{"title":"Biosynthesis of cupric oxide nanoparticles: its antiviral activities against TMV by directly destroying virion and inducing plant resistance","authors":"Shuaikang Liu, Weiqiang Tian, Zhongwei Liu, Xuefeng Wei, Kai Yuan, Wei Du, S.H. Chen, Shuhan Chen, Dong Zhou, Lin Cai","doi":"10.1186/s42483-024-00250-z","DOIUrl":"https://doi.org/10.1186/s42483-024-00250-z","url":null,"abstract":"Abstract Tobacco mosaic virus (TMV) is widely recognized as one of the most important plant viruses, causing significant agricultural losses in terms of both quality and yield worldwide each year. This study demonstrated the biosynthesis of copper oxide nanoparticles (CuONPs) using orange peel extract for effective control of TMV infection both in vitro and in vivo. After treatment with CuONPs (100 mg/L) for 2 h, TMV particles exhibited evident fragmentation in vitro, reducing infectivity on tobacco plants. Similarly, the application of CuONPs on Nicotiana benthamiana ( N . benthamiana ) positively impeded viral replication and accumulation in vivo. Interestingly, the expression of systemic resistance-related genes (PR1, PR2, ERF1 , and JAZ3 ) in the host plant was up-regulated by CuONPs treatment, supporting that CuONPs activated plant immunity to inhibit TMV. Importantly, the application of CuONPs (100 mg/L) did not exhibit any toxic effects on tobacco and, instead, resulted in the promotion of chlorophyll content, as well as an increase in the fresh weight and dry weight of the plant when compared to the control treatment. Overall, we proposed that the appropriate concentration of CuONPs (100 mg/L) can directly break viral particles by passivating, boost plant immunity by stimulating systemic acquired resistance (SAR), and provide nutritional supplements to promote plant growth.","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://phytopatholres.biomedcentral.com/counter/pdf/10.1186/s42483-024-00250-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147908047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Banana bunchy top virus movement protein induces resistance in banana against Fusarium wilt 香蕉束顶病毒运动蛋白诱导香蕉抗枯萎病

2区农林科学

Phytopathology Research Pub Date : 2024-04-30 DOI: 10.1186/s42483-024-00242-z

Weiying Wang, Wenqiang Wan, Qian Chen, Tàiyún Wèi, H. Q. Zhang

{"title":"Banana bunchy top virus movement protein induces resistance in banana against Fusarium wilt","authors":"Weiying Wang, Wenqiang Wan, Qian Chen, Tàiyún Wèi, H. Q. Zhang","doi":"10.1186/s42483-024-00242-z","DOIUrl":"https://doi.org/10.1186/s42483-024-00242-z","url":null,"abstract":"Abstract Banana Fusarium wilt, which is known as Panama disease and caused by Fusarium oxysporum f. sp. cubense ( Foc ), is a destructive disease that can lead to plant death and complete loss of banana plantations. For obtaining resistant varieties against Fusarium wilt, research is required to elucidate the mechanisms of genetic resistance to Foc . In this research, the banana plants infected with banana bunchy top virus (BBTV) showed resistance to Foc . RNA-seq results showed that the infection with Foc upregulated many resistance-related genes in BBTV-positive plants, especially genes related to ROS production. It was also found that more H 2 O 2 in BBTV-positive plants was induced during Foc infection. The movement protein (MP) of BBTV could increase H 2 O 2 levels by promoting the accumulation of MaSGT1a (suppressor of the G-two allele of SKP1) via direct interaction. Thus, the resistance to Foc induced by BBTV may attribute to MP increasing MaSGT1a protein levels to induce ROS production. MaSGT1a could promote the accumulation of R proteins MaRPM1 and MaRPP8, and increase H 2 O 2 levels in Nicotiana benthamiana . The expression of R proteins in Nicotiana benthamiana caused an increase in H 2 O 2 levels and promoted resistance to Foc . Thus, the MP of BBTV could induce plant defense through interaction with MaSGT1a. We speculate that MaSGT1a is likely involved in R gene-mediated resistance and thereby promotes the resistance of banana against Foc .","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147903317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Four sugarcane ScDIR genes contribute to lignin biosynthesis and disease resistance to Sporisorium scitamineum 甘蔗4个ScDIR基因参与木质素的生物合成和对甘蔗孢杆菌的抗性

2区农林科学

Phytopathology Research Pub Date : 2024-04-02 DOI: 10.1186/s42483-024-00237-w

Xiufang Li, Zongling Liu, Haoming Wu, Zhuo-Xin Yu, Jiaorong Meng, Haiyun Zhao, Xingli Deng, Yizu Su, Baoshan Chen, Ru Li

{"title":"Four sugarcane ScDIR genes contribute to lignin biosynthesis and disease resistance to Sporisorium scitamineum","authors":"Xiufang Li, Zongling Liu, Haoming Wu, Zhuo-Xin Yu, Jiaorong Meng, Haiyun Zhao, Xingli Deng, Yizu Su, Baoshan Chen, Ru Li","doi":"10.1186/s42483-024-00237-w","DOIUrl":"https://doi.org/10.1186/s42483-024-00237-w","url":null,"abstract":"Abstract Sugarcane ( Saccharum spp.) is a major sucrose and bioenergy crop in the world. The fungal pathogen Sporisorium scitamineum causes sugarcane smut, a devastating disease that destroys stalks and reduces sugar content in sugarcane. This disease can be controlled most effectively by applying smut-resistant sugarcane varieties. Previous studies have shown that Dirigent (DIR ) genes are involved in the synthesis of the lignin precursor pinoresinol, which plays a crucial role in plant resistance to biotic stresses. However, the immune response of the DIR homologs in sugarcane ( ScDIR ) has not been reported yet. In this study, we found that the lignin content of smut-resistant sugarcane varieties (ZZ1, ZZ6, and ZZ9) was significantly higher than that of smut-susceptible varieties (GT42, ROC22, and FN41), and the lignin content of sugarcane increased after smut infection. The smut-resistant and smut-susceptible clones derived from the same genetic population (ROC25 × YZ89-7) showed similar patterns. Quantitative real-time PCR assays revealed that among the 64 DIR genes in sugarcane, ScDIR5 , ScDIR7 , ScDIR11 , and ScDIR40 showed elevated expression after S. scitamineum infection. In vitro coupling reactions showed that the four corresponding ScDIR proteins could mediate the coupling of coniferyl alcohol and its conversion into the lignin precursor pinoresinol. Overexpression of the four ScDIR genes in Nicotiana benthamiana enhanced disease resistance to the fungal pathogens Sclerotium rolfsii , Rhizoctonia solani , and Botrytis cinerea . Moreover, transgenic sugarcane overexpressing these ScDIR genes showed enhanced resistance to smut disease. Taken together, our findings provide evidence that sugarcane ScDIR genes can improve the resistance of plants to fungal pathogens and highlight their potentials in sugarcane breeding for disease resistance.","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://phytopatholres.biomedcentral.com/counter/pdf/10.1186/s42483-024-00237-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147893287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Uncovering the mechanisms underlying pear leaf apoplast protein-mediated resistance against Colletotrichum fructicola through transcriptome and proteome profiling 通过转录组和蛋白质组分析揭示梨叶片细胞质蛋白介导的抗果孢子菌机制

IF 3.4 2区农林科学

Phytopathology Research Pub Date : 2024-01-09 DOI: 10.1186/s42483-023-00220-x

Chenyang Han, Zhiyuan Su, Yan-cun Zhao, Chaohui Li, B. Guo, Qi Wang, Fengquan Liu, Shaoling Zhang

引用次数: 0

Scopoletin negatively regulates the HOG pathway and exerts antifungal activity against Botrytis cinerea by interfering with infection structures, cell wall, and cell membrane formation Scopoletin 负向调节 HOG 通路，并通过干扰感染结构、细胞壁和细胞膜的形成对灰霉病菌发挥抗真菌活性

IF 3.4 2区农林科学

Phytopathology Research Pub Date : 2024-01-02 DOI: 10.1186/s42483-023-00219-4

Xiaojun Yuan, Fugen Yang, Yameng Wang, Shaoxuan Li, Demeng Zhang, Wenxing Liang, Qianqian Yang

引用次数: 0

Epidemiological description of fire blight introduction patterns to Central Asia and the Caucasus region based on CRISPR spacer typing and genome analysis. 基于 CRISPR spacer 分型和基因组分析对中亚和高加索地区火疫病传入模式的流行病学描述。

IF 3.2 2区农林科学

Phytopathology Research Pub Date : 2024-01-01 Epub Date: 2024-11-20 DOI: 10.1186/s42483-024-00283-4

Fabio Rezzonico, Saykal Bobushova, Dali Gaganidze, Mahabat Konurbaeva, Sergey Mukhanov, Sara Jordan, Tinatin Sadunishvili, Nataliya Drenova, Theo H M Smits, Tinatin Doolotkeldieva

{"title":"Epidemiological description of fire blight introduction patterns to Central Asia and the Caucasus region based on CRISPR spacer typing and genome analysis.","authors":"Fabio Rezzonico, Saykal Bobushova, Dali Gaganidze, Mahabat Konurbaeva, Sergey Mukhanov, Sara Jordan, Tinatin Sadunishvili, Nataliya Drenova, Theo H M Smits, Tinatin Doolotkeldieva","doi":"10.1186/s42483-024-00283-4","DOIUrl":"10.1186/s42483-024-00283-4","url":null,"abstract":"In the last two decades, fire blight has progressively spread eastward from Europe and the Mediterranean area to several pome-fruit producing regions of Asia. Its causative agent, the bacterial pathogen Erwinia amylovora, was detected in several new countries, including Georgia, Kyrgyzstan, and Kazakhstan. In the latter two states, the disease creates a threat not only to the commercial apple and pear production, but also to the wild Malus and Pyrus species that constitute the basis of the local forest ecosystems. In this study, we investigated the genetic diversity of the pathogen in Central Asia and the Caucasus region utilizing CRISPR Repeat Regions (CRRs) genotyping and genome sequencing, with the aim to understand its dissemination patterns across the continent. Genome sequence analysis revealed that all strains from these two regions exclusively derived from the archetypal CRR1 genotype A. Our analysis revealed three main E. amylovora clades in Central Asia, with distinct yet partial overlapping geographical distributions. Genomic relationships among isolates indicate that Central Asian strains are genetically closest to those from the Persian region and the Middle East, while the Georgian population is genetically more distant and can align with strains from the Volga District in southern Russia and the Eastern Mediterranean area. Notably, this study also includes strains from the first confirmed occurrences of fire blight in Uzbekistan, Tajikistan, and China. Our findings highlight the importance of phylogenetic analysis and genome sequencing in understanding the phytopathogen epidemics and protecting key agricultural species and the genetic resources of their wild counterparts in the forest.","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"6 1","pages":"66"},"PeriodicalIF":3.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11576639/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0