Pengbai Li, Ke Li, C. Jing, Rui Wu, Gentu Wu, Mingjun Li, L. Qing
{"title":"Molecular characterization of a novel Conyza canadensis-infecting begomovirus in China","authors":"Pengbai Li, Ke Li, C. Jing, Rui Wu, Gentu Wu, Mingjun Li, L. Qing","doi":"10.1186/s42483-022-00118-0","DOIUrl":"https://doi.org/10.1186/s42483-022-00118-0","url":null,"abstract":"","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2022-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47624751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Feng He, Jie Yang, Yan-cun Zhao, P. Laborda, Yifan Jia, Asma Safdar, A. Kange, Bingxin Li, Lan Zhou, Q. Zeng, Sally Brown, Z. Fu, Fengquan Liu
{"title":"Identification and characterization of a stem canker and twig dieback disease of pear caused by Neofusicoccum parvum in Chinese mainland","authors":"Feng He, Jie Yang, Yan-cun Zhao, P. Laborda, Yifan Jia, Asma Safdar, A. Kange, Bingxin Li, Lan Zhou, Q. Zeng, Sally Brown, Z. Fu, Fengquan Liu","doi":"10.1186/s42483-022-00111-7","DOIUrl":"https://doi.org/10.1186/s42483-022-00111-7","url":null,"abstract":"","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2022-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44819062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yu Wang, Nan Yang, Yunna Zheng, Jiaolin Yue, V. Bhadauria, You-Liang Peng, Qianwang Chen
{"title":"Ubiquitination in the rice blast fungus Magnaporthe oryzae: from development and pathogenicity to stress responses","authors":"Yu Wang, Nan Yang, Yunna Zheng, Jiaolin Yue, V. Bhadauria, You-Liang Peng, Qianwang Chen","doi":"10.1186/s42483-021-00106-w","DOIUrl":"https://doi.org/10.1186/s42483-021-00106-w","url":null,"abstract":"","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"4 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2022-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65793806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Phytopathology ResearchPub Date : 2022-01-01Epub Date: 2022-06-20DOI: 10.1186/s42483-022-00128-y
Xueyan Duan, Wendi Ma, Zhiyuan Jiao, Yiying Tian, Ragab Gomaa Ismail, Tao Zhou, Zaifeng Fan
{"title":"Reverse transcription-recombinase-aided amplification and CRISPR/Cas12a-based visual detection of maize chlorotic mottle virus.","authors":"Xueyan Duan, Wendi Ma, Zhiyuan Jiao, Yiying Tian, Ragab Gomaa Ismail, Tao Zhou, Zaifeng Fan","doi":"10.1186/s42483-022-00128-y","DOIUrl":"https://doi.org/10.1186/s42483-022-00128-y","url":null,"abstract":"<p><p>Maize chlorotic mottle virus (MCMV) is one of the important quarantine pathogens in China. It often co-infects with one or two viruses in the family <i>Potyviridae</i> and causes maize lethal necrosis disease. Therefore, an accurate and sensitive method for the detection of MCMV is urgently needed. Combined with reverse transcription and recombinase-aided amplification, we developed a CRISPR<b>/</b>Cas12a-based visual nucleic acid detection system targeting the MCMV coat protein gene. The whole process can be completed within 45 min with high sensitivity. This system could detect cDNAs diluted up to 10<sup>-5</sup> when 2000 ng of total RNA was used for reverse transcription. The Cas12a/crRNA complex designed for MCMV detection could recognize and cleave the targeted double-stranded DNA, and ultimately cleave the single-stranded DNA probes and produce fluorescent signals. The green fluorescence produced under blue light (440-460 nm) in this procedure could be observed by the naked eye. Since this novel method is specific, rapid, sensitive and does not require special instruments and technical expertise, it should be suitable for on-site visual detection of MCMV in seeds, plants of maize and potentially in its insect vectors.</p>","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"4 1","pages":"23"},"PeriodicalIF":3.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40401949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of potato virus X resistance in potato cultivars and identification of an innate immunity-independent resistance phenotype","authors":"Jiahui Liu, Ye Liu, Yue Fang, Lili Zhang, Kai-Kang Yu, Xiaoyun Wu, Xiaofei Cheng","doi":"10.1186/s42483-021-00099-6","DOIUrl":"https://doi.org/10.1186/s42483-021-00099-6","url":null,"abstract":"","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2021-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45061691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhiyuan Jiao, Juan Wang, Yiying Tian, Siyuan Wang, Xi Sun, Siqi Li, Wendi Ma, T. Zhou, Z. Fan
{"title":"Maize catalases localized in peroxisomes support the replication of maize chlorotic mottle virus","authors":"Zhiyuan Jiao, Juan Wang, Yiying Tian, Siyuan Wang, Xi Sun, Siqi Li, Wendi Ma, T. Zhou, Z. Fan","doi":"10.1186/s42483-021-00094-x","DOIUrl":"https://doi.org/10.1186/s42483-021-00094-x","url":null,"abstract":"","PeriodicalId":20098,"journal":{"name":"Phytopathology Research","volume":"3 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2021-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42483-021-00094-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65793730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}