Pflugers Archiv最新文献

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Pflugers Archiv Pub Date : 2024-04-16 DOI: 10.1007/s00424-024-02963-8

Andreas Draguhn, Armin Kurtz

引用次数: 0

Correction to: Liraglutide versus pramlintide in protecting against cognitive function impairment through affecting PI3K/AKT/GSK‑3β/TTBK1 pathway and decreasing Tau hyperphosphorylation in high‑fat dietstreptozocin rat model 更正：利拉鲁肽与普兰林肽通过影响PI3K/AKT/GSK-3β/TTBK1通路和降低高脂饮食大鼠模型中Tau过度磷酸化来防止认知功能损伤的比较

Pflugers Archiv Pub Date : 2024-04-05 DOI: 10.1007/s00424-024-02959-4

Hoda M. Moghazy, Nesreen G. Abdelhaliem, Sherine Ahmed Mohammed, Asmaa Hassan, Amany Abdelrahman

引用次数: 0

AFM-based nanoindentation indicates an impaired cortical stiffness in the AAV-PCSK9^DY atherosclerosis mouse model. 在AAV-PCSK9DY动脉粥样硬化小鼠模型中，基于afm的纳米压痕显示皮质刚度受损。

Pflugers Archiv Pub Date : 2022-09-01 Epub Date: 2022-06-01 DOI: 10.1007/s00424-022-02710-x

Leonie Achner, Tobias Klersy, Benedikt Fels, Tobias Reinberger, Cosima X Schmidt, Natalie Groß, Susanne Hille, Oliver J Müller, Zouhair Aherrahrou, Kristina Kusche-Vihrog, Walter Raasch

{"title":"AFM-based nanoindentation indicates an impaired cortical stiffness in the AAV-PCSK9DY atherosclerosis mouse model.","authors":"Leonie Achner, Tobias Klersy, Benedikt Fels, Tobias Reinberger, Cosima X Schmidt, Natalie Groß, Susanne Hille, Oliver J Müller, Zouhair Aherrahrou, Kristina Kusche-Vihrog, Walter Raasch","doi":"10.1007/s00424-022-02710-x","DOIUrl":"10.1007/s00424-022-02710-x","url":null,"abstract":"Investigating atherosclerosis and endothelial dysfunction has mainly become established in genetically modified ApoE-/- or LDL-R-/- mice transgenic models. A new AAV-PCSK9DYDY mouse model with no genetic modification has now been reported as an alternative atherosclerosis model. Here, we aimed to employ this AAV-PCSK9DY mouse model to quantify the mechanical stiffness of the endothelial surface, an accepted hallmark for endothelial dysfunction and forerunner for atherosclerosis. Ten-week-old male C57BL/6 N mice were injected with AAV-PCSK9DY (0.5, 1 or 5 × 1011 VG) or saline as controls and fed with Western diet (1.25% cholesterol) for 3 months. Total cholesterol (TC) and triglycerides (TG) were measured after 6 and 12 weeks. Aortic sections were used for atomic force microscopy (AFM) measurements or histological analysis using Oil-Red-O staining. Mechanical properties of in situ endothelial cells derived from ex vivo aorta preparations were quantified using AFM-based nanoindentation. Compared to controls, an increase in plasma TC and TG and extent of atherosclerosis was demonstrated in all groups of mice in a viral load-dependent manner. Cortical stiffness of controls was 1.305 pN/nm and increased (10%) in response to viral load (≥ 0.5 × 1011 VG) and positively correlated with the aortic plaque content and plasma TC and TG. For the first time, we show changes in the mechanical properties of the endothelial surface and thus the development of endothelial dysfunction in the AAV-PCSK9DY mouse model. Our results demonstrate that this model is highly suitable and represents a good alternative to the commonly used transgenic mouse models for studying atherosclerosis and other vascular pathologies.","PeriodicalId":20032,"journal":{"name":"Pflugers Archiv","volume":"22 1","pages":"993-1002"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9393126/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85394997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of the soluble epoxide hydrolase in the hair follicle stem cell homeostasis and hair growth. 可溶性环氧化物水解酶在毛囊干细胞稳态和毛发生长中的作用。

Pflugers Archiv Pub Date : 2022-09-01 Epub Date: 2022-06-01 DOI: 10.1007/s00424-022-02709-4

Zumer Naeem, Sven Zukunft, Stephan Günther, Stefan Liebner, Andreas Weigert, Bruce D Hammock, Timo Frömel, Ingrid Fleming

{"title":"Role of the soluble epoxide hydrolase in the hair follicle stem cell homeostasis and hair growth.","authors":"Zumer Naeem, Sven Zukunft, Stephan Günther, Stefan Liebner, Andreas Weigert, Bruce D Hammock, Timo Frömel, Ingrid Fleming","doi":"10.1007/s00424-022-02709-4","DOIUrl":"10.1007/s00424-022-02709-4","url":null,"abstract":"Polyunsaturated fatty acids (PUFAs) are used as traditional remedies to treat hair loss, but the mechanisms underlying their beneficial effects are not well understood. Here, we explored the role of PUFA metabolites generated by the cytochrome P450/soluble epoxide hydrolase (sEH) pathway in the regulation of the hair follicle cycle. Histological analysis of the skin from wild-type and sEH-/- mice revealed that sEH deletion delayed telogen to anagen transition, and the associated activation of hair follicle stem cells. Interestingly, EdU labeling during the late anagen stage revealed that hair matrix cells from sEH-/- mice proliferated at a greater rate which translated into increased hair growth. Similar effects were observed in in vitro studies using hair follicle explants, where a sEH inhibitor was also able to augment whisker growth in follicles from wild-type mice. sEH activity in the dorsal skin was not constant but altered with the cell cycle, having the most prominent effects on levels of the linoleic acid derivatives 12,13-epoxyoctadecenoic acid (12,13-EpOME), and 12,13-dihydroxyoctadecenoic acid (12,13-DiHOME). Fitting with this, the sEH substrate 12,13-EpOME significantly increased hair shaft growth in isolated anagen stage hair follicles, while its diol; 12,13-DiHOME, had no effect. RNA sequencing of isolated hair matrix cells implicated altered Wnt signaling in the changes associated with sEH deletion. Taken together, our data indicate that the activity of the sEH in hair follicle changes during the hair follicle cycle and impacts on two stem cell populations, i.e., hair follicle stem cells and matrix cells to affect telogen to anagen transition and hair growth.","PeriodicalId":20032,"journal":{"name":"Pflugers Archiv","volume":"25 1","pages":"1021-1035"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9393123/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78588492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flexible and multifaceted: the plasticity of renin-expressing cells. 灵活和多面性：肾素表达细胞的可塑性。

Pflugers Archiv Pub Date : 2022-08-01 Epub Date: 2022-05-05 DOI: 10.1007/s00424-022-02694-8

Katharina A E Broeker, Julia Schrankl, Michaela A A Fuchs, Armin Kurtz

引用次数: 0

Renal water transport in health and disease 肾水运在健康与疾病中的作用

Pflugers Archiv Pub Date : 2022-06-09 DOI: 10.1007/s00424-022-02712-9

E. Feraille, A. Sassi, V. Olivier, Grégoire Arnoux, Pierre-Yves Martin

引用次数: 3

Correction to: Two adjacent phosphorylation sites in the C-terminus of the channel’s α-subunit have opposing effects on epithelial sodium channel (ENaC) activity 更正:通道α-亚基c端两个相邻的磷酸化位点对上皮钠通道(ENaC)活性有相反的影响

Pflugers Archiv Pub Date : 2022-06-09 DOI: 10.1007/s00424-022-02717-4

A. Diakov, V. Nesterov, A. Dahlmann, C. Korbmacher