Pesquisa Veterinaria Brasileira最新文献_第2页

Vitamin A deficiency as a cause of blindness in feedlot calves 维生素A缺乏是饲养场小牛失明的原因

IF 0.6 4区农林科学

Pesquisa Veterinaria Brasileira Pub Date : 2023-06-30 DOI: 10.1590/1678-5150-pvb-7249

R. C. Pupin, Larissa L. Souza, Thaísa X. Silva, H. Fonseca, Eduarda do Amaral Souza Silva, D. Gomes, M. A. Araújo, R. Lemos

{"title":"Vitamin A deficiency as a cause of blindness in feedlot calves","authors":"R. C. Pupin, Larissa L. Souza, Thaísa X. Silva, H. Fonseca, Eduarda do Amaral Souza Silva, D. Gomes, M. A. Araújo, R. Lemos","doi":"10.1590/1678-5150-pvb-7249","DOIUrl":"https://doi.org/10.1590/1678-5150-pvb-7249","url":null,"abstract":"ABSTRACT: Vitamin A is an essential micronutrient to reproduction, development, normal growth, and function of the nervous system and vision, especially in growing animals. Hypovitaminosis A can affect any of these functions. The aim of this study was to describe the epidemiology, clinical signs, pathological aspects, and diagnostic approach in an outbreak of blindness in feedlot calves fed only grain. Five of 57 ten-month-old calves became blind after eight months at the feedlot. Clinical signs were mydriasis, absent pupillary and menace reflexes, bilaterally. Hypovitaminosis A was suspected and subsequently confirmed by low serum levels of vitamin A. One calf was euthanized and necropsied, and no gross findings were present. Histopathological lesions were restricted to the retina and characterized by degeneration and atrophy of the photoreceptor layer and some retinal scars. The calves were treated with intramuscular vitamin A injection at a dose of 5,000 international units (IU) per kilogram of body weight (kg BW) every 60 days until slaughter. Affected calves did not recover visual capacity, but no other cases occurred.","PeriodicalId":19991,"journal":{"name":"Pesquisa Veterinaria Brasileira","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67519641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of phosphorus levels in bones and serum of buffaloes (Bubalus bubalis) before and after supplementation with a selective mineral mixture 补充选择性矿物混合物前后水牛(Bubalus bubalis)骨骼和血清中磷水平的评价

IF 0.6 4区农林科学

Pesquisa Veterinaria Brasileira Pub Date : 2023-05-29 DOI: 10.1590/1678-5150-pvb-7130

C.M.C. Oliveira, H. A. Bomjardim, N. Silveira, José Alcides S. Silveira, Kelson C.F. Faial, E. S. Silva Filho, F. M. Salvarani, J. D. Barbosa

{"title":"Evaluation of phosphorus levels in bones and serum of buffaloes (Bubalus bubalis) before and after supplementation with a selective mineral mixture","authors":"C.M.C. Oliveira, H. A. Bomjardim, N. Silveira, José Alcides S. Silveira, Kelson C.F. Faial, E. S. Silva Filho, F. M. Salvarani, J. D. Barbosa","doi":"10.1590/1678-5150-pvb-7130","DOIUrl":"https://doi.org/10.1590/1678-5150-pvb-7130","url":null,"abstract":"ABSTRACT: This study aimed to evaluate phosphorus (P) concentration in serum and bone, the percentage of ash and the specific bone density of buffaloes on Ilha de Marajó before and after mineral supplementation. For this study, 14 crossbred buffaloes of Murrah and Mediterranean descent aged between 18 and 36 months were used. The average values of P before supplementation in serum and bone, the percentage of bone ash and the specific bone density were 5.68mg/dL±1.18, 16.53%±0.53, 59.95%±1.96 and 1.52g/cm3±0.32, respectively, which demonstrated P deficiency in animals raised on Ilha de Marajó. After supplementation with P for a period of seven months, the values were 6.61mg/dL±0.87, 16.90%±0.56 and 60.30%±0.95 and 1.71g/cm3±0.21, respectively. These results showed a significant increase in P concentration in blood serum, specific bone density and percentage of P in ash (P<0.05), but there was no significant increase in the percentage of ash. The average increase in P in the serum and ash did not reach normal levels in all animals; however, 28.6% of the animals had normal values of P in serum and 50% in the ash, and 64.3% had normal specific bone density values. The nonre-establishment, in some of the animals, of the variables of P serum and bone after supplementation for seven months may have occurred as a result of the low intake of the mineral mixture and by the low concentration of P in the Brachiaria brizantha cv. Marandu used for feeding animals during the experiment.","PeriodicalId":19991,"journal":{"name":"Pesquisa Veterinaria Brasileira","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67518626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathology, microbiology, and molecular evaluation of tissues from equids serologically positive for Burkholderia mallei in Midwestern Brazil 巴西中西部马氏伯克氏菌血清学阳性马科马组织的病理学、微生物学和分子评价

IF 0.6 4区农林科学

Pesquisa Veterinaria Brasileira Pub Date : 2023-05-29 DOI: 10.1590/1678-5150-pvb-7172

Leandro S. Rocha, Aruaque L.F. Oliveira, Felipe P. Arruda, L. C. Pitchenin, Valéria Dutra, Luciano Nakazato, F. Furlan, E. M. Colodel

{"title":"Pathology, microbiology, and molecular evaluation of tissues from equids serologically positive for Burkholderia mallei in Midwestern Brazil","authors":"Leandro S. Rocha, Aruaque L.F. Oliveira, Felipe P. Arruda, L. C. Pitchenin, Valéria Dutra, Luciano Nakazato, F. Furlan, E. M. Colodel","doi":"10.1590/1678-5150-pvb-7172","DOIUrl":"https://doi.org/10.1590/1678-5150-pvb-7172","url":null,"abstract":"ABSTRACT: Glanders is a disease caused by the bacterium Burkholderia mallei that primarily affects horses, mules and donkeys. The disease can cause lesions in the skin, lungs and several other organs. However, it often manifests as an asymptomatic disease. In Brazil, serological tests of high sensitivity and specificity are used to assist in the detection of antibodies against B. mallei and to contribute to the control of the disease. However, due to the mandatory euthanasia of seroreactive animals, equids with positive serology for B. mallei and asymptomatic generated great conflicts between breeders, veterinarians and diagnostic laboratories. This study clarifies the limitations of complementary diagnostic tests for detecting B. mallei. It describes the clinical, morphological and laboratory findings in 24 equines from different municipalities in the Mato Grosso State, Brazil, which reacted to the complement fixation test and were positive in the western blotting test for glanders. Data and tissue samples were collected from 24 horses for histological, microbiological and molecular analysis. In 23 horses, no clinical signs, morphological alterations, microbiological isolation, or molecular detection would characterize B. mallei infection. On the other hand, samples from an asymptomatic horse without lesional alterations showed sequence amplification compatible with B. mallei in the PCR. Considering that the infection by B. mallei is subject to the application of animal sanitary defense measures and that, by international requirement and national legislation, the serological results are tools that should support the sanitation procedures for the error of the bacteria in the Mato Grosso State, Brazil.","PeriodicalId":19991,"journal":{"name":"Pesquisa Veterinaria Brasileira","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67519101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Main diseases of cattle in the midwestern region of São Paulo state <s:1>圣保罗州中西部地区牛的主要疾病

IF 0.6 4区农林科学

Pesquisa Veterinaria Brasileira Pub Date : 2023-05-08 DOI: 10.1590/1678-5150-pvb-7216

Cristiana R. Bromberger, João Pedro M. Oliveira, Ana Maria D. Costa, R. M. Amorim, A. S. Borges, J. P. Oliveira-Filho

{"title":"Main diseases of cattle in the midwestern region of São Paulo state","authors":"Cristiana R. Bromberger, João Pedro M. Oliveira, Ana Maria D. Costa, R. M. Amorim, A. S. Borges, J. P. Oliveira-Filho","doi":"10.1590/1678-5150-pvb-7216","DOIUrl":"https://doi.org/10.1590/1678-5150-pvb-7216","url":null,"abstract":"ABSTRACT: São Paulo state is one of the country’s largest producers of beef and milk, and the midwestern region plays a key role in this production, as half of São Paulo’s cattle herd is found in this region. These numbers alone demonstrate the importance of livestock in this region. Therefore, this study aimed to describe the main epidemiological and clinical signs in cattle cases at the Large Animal Hospital at FMVZ-Unesp, located in the midwestern region of São Paulo state. The present retrospective study assessed 638 clinical cases of cattle treated from January 2010 to December 2019 (10 years). Digestive system diseases were the most prevalent, diagnosed in 30.3% of patients, followed by neurological diseases (19.1%) and respiratory diseases (10.5%). The other diseases were distributed in decreasing order as follows: musculoskeletal (7.8%), hematopoietic (6.1%), genitourinary (5.6%), metabolic and nutritional (5.5%), neonatal (4.7%), cutaneous (2.6%), poisoning (2.5%), lymphatic (2.2%), cardiovascular (1.6%) and other diseases (1.4%). Rabies, a fatal zoonotic disease, was the main cause of death in this study and the main disease associated with neurological signs (23.7%). Recognizing the main diseases of cattle in this region will promote the adoption of prophylactic measures to minimize their occurrence and manage treatment to avoid economic losses and decreased productivity of herds.","PeriodicalId":19991,"journal":{"name":"Pesquisa Veterinaria Brasileira","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67519161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cutaneous pythiosis in equines in the Amazon Biome 亚马逊生物群中马的皮癣病

IF 0.6 4区农林科学

Pesquisa Veterinaria Brasileira Pub Date : 2023-04-28 DOI: 10.1590/1678-5150-pvb-7167

J. D. Barbosa, Hanna Gabriela da Silva Oliveira, S. M. Bosco, N. Silveira, Camila C. Barbosa, M. F. Brito, C.M.C. Oliveira, F. M. Salvarani

{"title":"Cutaneous pythiosis in equines in the Amazon Biome","authors":"J. D. Barbosa, Hanna Gabriela da Silva Oliveira, S. M. Bosco, N. Silveira, Camila C. Barbosa, M. F. Brito, C.M.C. Oliveira, F. M. Salvarani","doi":"10.1590/1678-5150-pvb-7167","DOIUrl":"https://doi.org/10.1590/1678-5150-pvb-7167","url":null,"abstract":"ABSTRACT: The study aimed to describe the clinicopathological aspects of 37 cases of pythiosis, 34 in horses and three in mules, from properties located in the Amazon biome of Pará, Brazil. The clinical signs observed in the animals were weakness, poor-to-regular nutritional status, pale mucous membranes, itching at the lesion site, and lameness when the limbs were affected. The lesions were located on the lips, nostrils, rib region, thoracic and abdominal walls, scapular, distal limbs, foreskin, perineum and udder. Macroscopically, ulcerative and granulation-tissue-like masses were observed, with fistulous tracts filled with yellowish and foul-smelling serosanguinous discharges. In the biopsy, it was possible to visualize white and firm areas with foci of yellowish necrotic material and hardened masses, called kunkers. The histopathological examination showed a pyogranulomatous inflammatory reaction with the presence of Pythium insidiosum hyphae, which were impregnated with black, confirming the diagnosis of pythiosis in equids in the Amazon biome, being the first report of the disease in mules in the region.","PeriodicalId":19991,"journal":{"name":"Pesquisa Veterinaria Brasileira","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67519090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Increased frequency of pneumonia in dogs with meningioma in ventral rhombencephalon 腹侧斜脑膜瘤犬患肺炎的频率增加

IF 0.6 4区农林科学

Pesquisa Veterinaria Brasileira Pub Date : 2023-04-21 DOI: 10.1590/1678-5150-pvb-7114

B. Almeida, L. Henker, M. Bianchi, S. Pavarini, L. Sonne, D. Driemeier

{"title":"Increased frequency of pneumonia in dogs with meningioma in ventral rhombencephalon","authors":"B. Almeida, L. Henker, M. Bianchi, S. Pavarini, L. Sonne, D. Driemeier","doi":"10.1590/1678-5150-pvb-7114","DOIUrl":"https://doi.org/10.1590/1678-5150-pvb-7114","url":null,"abstract":"ABSTRACT: Intracranial tumors occurring in specific brain regions, such as the cerebellopontine angle, may be associated with cranial nerve dysfunction and dysphagia in humans and animals. Although dysphagia is a known risk factor for pneumonia, only postoperative pneumonia has been investigated in veterinary medicine. This study aimed to describe the clinical and pathological features of dogs with untreated intracranial meningiomas and concomitant pneumonia. Data from post-mortem examination registries from 2011 to 2021 were used (n=23). The frequency of pneumonia and other characteristics were compared between dogs with meningiomas in the ventral rhombencephalon region (VR group; n=13) and those with meningiomas in other intracranial sites (OIS group; n=10). The frequency of pneumonia was higher in the VR group than in the OIS group (n=5 vs. n=0; P=0.039). Plaque-like lesions were also more common in the VR group than in the OIS group (P=0.012). Dogs with concomitant pneumonia had cerebellopontine angle (n=3) and basilar meningiomas (n=2), mainly plaque-like lesions extending to or from other brain areas. In dogs with concomitant pneumonia, meningiomas had invasive (n=5) and compressive (n=3) growth behaviors and nerve roots involved in the swallowing process were frequently affected. Microscopically, these meningiomas were classified as atypical (n=4) and meningiomas (n=1). The reported clinical signs included anorexia (n=3), adipsia (n=1), and dysphagia (n=1). Our findings suggest untreated dogs with ventral rhombencephalon meningiomas may develop cranial nerve damage and aspiration pneumonia.","PeriodicalId":19991,"journal":{"name":"Pesquisa Veterinaria Brasileira","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67518801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retrospective evaluation of canine anal sac carcinoma (260 cases) in Brazil: late detection and failures in lymph node evaluation 巴西犬肛囊癌(260例)的回顾性分析:淋巴结评估的晚期发现和失败

IF 0.6 4区农林科学

Pesquisa Veterinaria Brasileira Pub Date : 2023-04-21 DOI: 10.1590/1678-5150-pvb-7210

F. N. Paiva, S. Moreira, Laís Calazans Menescal Linhares, F. Sueiro, A. B. Nardi, P. Jark

{"title":"Retrospective evaluation of canine anal sac carcinoma (260 cases) in Brazil: late detection and failures in lymph node evaluation","authors":"F. N. Paiva, S. Moreira, Laís Calazans Menescal Linhares, F. Sueiro, A. B. Nardi, P. Jark","doi":"10.1590/1678-5150-pvb-7210","DOIUrl":"https://doi.org/10.1590/1678-5150-pvb-7210","url":null,"abstract":"ABSTRACT: Anal sac neoplasms are common in companion animals, and the epidemiological profile has been extensively described in international studies; however, national data are still lacking. Data on the Brazilian reality of anal sac carcinoma cases’ diagnosis and treatment are also scarce. The purpose of this study was to retrospectively evaluate cases of canine anal sac carcinoma and assess the profile of involvement, tumor size, and lymphatic invasion at the time of diagnosis. Information was obtained from Vetpat laboratory database, from 260 cases spanning a 12-year period (2010-2021). In histopathological evaluation at the time of diagnosis, data on sex, age, and race were described, as well as tumor size and lymphatic invasion. The presence of metastasis was also assessed in cases where lymph nodes were sampled. Simple descriptive statistical analysis was used to evaluate the data. Adult and elderly, female, and mixed-breed animals were more involved, indicating differences from international studies that can be attributed to sociocultural factors. In terms of tumor size, it was observed that 93% of the cases had the largest diameter above 2.5cm. Only 7% of the cases had the largest diameter below 2.5cm, demonstrating the often late diagnosis and the importance of rectal palpation examination during the general physical assessment of canine patients, particularly at an advanced age. Regarding lymphatic invasion and affected lymph nodes, 50% of the cases had lymphatic invasion described in the histopathological examination. However, only 5% of the lymph nodes were sent along with the primary tumor, indicating the disease’s aggressive behavior but with possible metastases underdiagnosed.","PeriodicalId":19991,"journal":{"name":"Pesquisa Veterinaria Brasileira","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67519059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serological frequency of Leptospira spp. in buffaloes (Bubalus bubalis) in Paraná state, Brazil 巴西帕拉那<e:1>州水牛钩端螺旋体的血清学频率

IF 0.6 4区农林科学

Pesquisa Veterinaria Brasileira Pub Date : 2023-04-21 DOI: 10.1590/1678-5150-pvb-7147

Louise Krueger, A. W. Biondo, L. B. Kmetiuk, Maria do Carmo C.S.H. Lara, V. Castro, Cristina C. Dib, Rodrigo A.M. Oliveira, João H. Perotta, I. R. B. Barros Filho

{"title":"Serological frequency of Leptospira spp. in buffaloes (Bubalus bubalis) in Paraná state, Brazil","authors":"Louise Krueger, A. W. Biondo, L. B. Kmetiuk, Maria do Carmo C.S.H. Lara, V. Castro, Cristina C. Dib, Rodrigo A.M. Oliveira, João H. Perotta, I. R. B. Barros Filho","doi":"10.1590/1678-5150-pvb-7147","DOIUrl":"https://doi.org/10.1590/1678-5150-pvb-7147","url":null,"abstract":"ABSTRACT: Leptospirosis is a zoonosis that can infect buffaloes and other mammalians, and it’s caused by Gram-negative bacteria of the genus Leptospira spp. The infection occurs through contact with urine or a contaminated environment with the mucous membranes of the animals, and the disease has two phases: leptospiremia and leptospiruria. The objective of this study was to determine the frequency of leptospirosis in buffaloes in the state of Paraná, Brazil, as well as the main serovars present in this area. A total of 276 blood samples were collected from buffaloes from 18 properties located in different cities and mesoregions of Paraná state. A microscopic agglutination test (MAT) was performed to detect the presence of anti-Leptospira spp. antibodies and to identify its serovars. The prevalence was 77.17% (213/276) of buffaloes seropositive for at least one serovar. The most frequent serovar was Icterohaemorrhagiae, followed by the Pomona serovar and serovar Hardjo. This study seems to be the first to determine the frequency of buffaloes with leptospirosis in the state of Paraná. Prevention and control measures are important to control the disease in herds.","PeriodicalId":19991,"journal":{"name":"Pesquisa Veterinaria Brasileira","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67518940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of smartphone messaging applications to increase diagnostic efficiency in veterinary diagnostic laboratories 使用智能手机短信应用程序提高兽医诊断实验室的诊断效率

IF 0.6 4区农林科学

Pesquisa Veterinaria Brasileira Pub Date : 2023-04-14 DOI: 10.1590/1678-5150-pvb-7226

R. Lemos, C. C. Guizelini, Thaísa X. Silva, Larissa L. Souza, Helena C.F. Fonseca, Gisele C. Bonato, D. Gomes, R. C. Pupin

{"title":"Use of smartphone messaging applications to increase diagnostic efficiency in veterinary diagnostic laboratories","authors":"R. Lemos, C. C. Guizelini, Thaísa X. Silva, Larissa L. Souza, Helena C.F. Fonseca, Gisele C. Bonato, D. Gomes, R. C. Pupin","doi":"10.1590/1678-5150-pvb-7226","DOIUrl":"https://doi.org/10.1590/1678-5150-pvb-7226","url":null,"abstract":"ABSTRACT: Many inconclusive diagnoses have been reported in studies carried out in veterinary diagnostic laboratories (VDLs). These inconclusive diagnoses are often associated with field veterinarians sending tissue samples and carcasses for histopathological examination. The use of social media in care has the potential as a tool for improvements in animal health. We evaluated the improvement in the rate of conclusive diagnoses in the VDL when using a messaging application for smartphones as a guidance tool for field veterinarians to refer animals and sample tissues for necropsy and histopathology. Veterinarians contacted the VDL team via a messaging app and forwarded videos, images, and clinical-epidemiological history. Based on this information, guidelines were created in real-time for contacting professionals about methods for collecting samples during necropsy and making diagnoses and scheduling visits to properties. The data obtained showed that smartphone messaging applications could be useful tools to expand the performance of VDLs and improve their diagnostic efficiency, especially when sending samples of animals that died is impossible due to certain sanitary conditions.","PeriodicalId":19991,"journal":{"name":"Pesquisa Veterinaria Brasileira","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67519903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Outbreak of leishmaniasis caused by Leishmania enriettii in guinea pigs (Cavia porcellus) 豚鼠中由埃利什曼原虫引起的利什曼病暴发

IF 0.6 4区农林科学

Pesquisa Veterinaria Brasileira Pub Date : 2023-04-14 DOI: 10.1590/1678-5150-pvb-7241

Cibele Ribeiro, Kethleen N. Koubiach, L. S. Faccini, M. Teixeira, Fábio A. Schüür, V. Thomaz-Soccol, C. Barros, A. Coelho

{"title":"Outbreak of leishmaniasis caused by Leishmania enriettii in guinea pigs (Cavia porcellus)","authors":"Cibele Ribeiro, Kethleen N. Koubiach, L. S. Faccini, M. Teixeira, Fábio A. Schüür, V. Thomaz-Soccol, C. Barros, A. Coelho","doi":"10.1590/1678-5150-pvb-7241","DOIUrl":"https://doi.org/10.1590/1678-5150-pvb-7241","url":null,"abstract":"ABSTRACT: We describe an outbreak of leishmaniasis in seven guinea pigs (Cavia porcellus) in which nodular ulcerated skin lesions of varying sizes were observed in the nasal cavity, upper lip, pinnae, vulva, and periarticular region of the limbs. Cytologic exam of collected samples of the lesions in the auricle of one of the animals revealed macrophages containing parasitophorous vacuoles of approximately 4.0μm in diameter in their cytoplasm with morphology suggestive of Leishmania sp. Although skin lesions spontaneously regressed in two of the Guinea pigs, only one survived. All six animals that died were necropsied. Grossly, all animals showed bloody nodular cutaneous lesions with crusts. One of the guinea pigs had distended dark red and firm lungs. Histopathology of the skin lesions revealed histiocytic interstitial acanthotic dermatitis associated with a myriad of Leishmania organisms within macrophages cytoplasm. In the lung, the lesions were characteristic of broncho-interstitial pneumonia with focal infiltrates of neutrophils, epithelioid macrophages, and multinucleated giant cells containing 2µm basophilic amastigotes with morphology compatible with Leishmania spp. A focal granulomatous lesion ,associated with the causal agent in the lung is a novel description of leishmaniasis in guinea pigs caused by L. enriettii. The polymerase chain reaction (PCR) technique with mini-exon primer performed in samples of lesions from two affected guinea pigs was positive and equal to the reference strain, identifying Leishmania enriettii. The cytological, macroscopic, and histological lesions associated with the PCR technique allowed the diagnosis of leishmaniasis and the identification of the specie L. enriettii.","PeriodicalId":19991,"journal":{"name":"Pesquisa Veterinaria Brasileira","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67519557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0