Periodicum Biologorum最新文献_第5页

Polyethylenimine as a gene delivery tool in triple-negative breast cancer cell line and breast cancer stem cell model 聚乙烯亚胺在三阴性乳腺癌细胞系和乳腺癌干细胞模型中的基因传递工具

IF 0.3 4区生物学

Periodicum Biologorum Pub Date : 2022-07-01 DOI: 10.18054/pb.v123i3-4.10472

M. Matovina, Steffi Lemmens, M. Kralj, K. Ester

{"title":"Polyethylenimine as a gene delivery tool in triple-negative breast cancer cell line and breast cancer stem cell model","authors":"M. Matovina, Steffi Lemmens, M. Kralj, K. Ester","doi":"10.18054/pb.v123i3-4.10472","DOIUrl":"https://doi.org/10.18054/pb.v123i3-4.10472","url":null,"abstract":"Background and purpose: PEI is a cationic polymer able to neutralise negative DNA charges and to condense large genes which makes it suitable for gene delivery in human cells. Despite its low cost, simplicity of use and moderate toxicity, there is still room for broader usage and experimental adjustments, especially in cell lines that are difficult to transfect. In the presented research, we used PEI for the delivery of plasmid DNA into TNBC cell line SUM159 and breast CSC model HMLE-Twist.Material and methods: Cultured cells were transfected with GFP-expressing plasmid using both PEI and Lipofectamin. Transfection efficiency was determined by flow cytometry measurements of the intensity of the green fluorescence, while viability was determined by measuring intensity of the red fluorescence after propidium iodide staining. Results: In SUM159 and HMLE-Twist cells we obtain transfection efficiency between 30-40% using PEI, while cytotoxicity was generally low to moderate. PEI caused 10% of cell death in SUM159 and 20 % in HMLE-Twist. Transfection efficiency of PEI was comparable and even higher than the efficiency of the Lipofectamine in both SUM159 and HMLE-Twist. In mammary epithelia (control HMLE) we obtained only 20 % transfection efficiency using both carriers.Conclusions: We demonstrated for the first time that PEI represents a suitable nanocarrier for gene delivery into breast CSC model. We successfully transfected both breast CSC model HMLE-Twist and TNBC line SUM159. Since PEI is inexpensive and easy to use, we recommend it for further exploitations of these cell lines in TNBC research.","PeriodicalId":19950,"journal":{"name":"Periodicum Biologorum","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46886211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disinfectant as Removal Agent of the Pre-Formed Biofilm by Staphylococcus sp. Isolated from Dental Clinics in Taif, KSA 消毒剂作为去除KSA Taif牙科诊所分离的葡萄球菌预先形成的生物膜的试剂

IF 0.3 4区生物学

Periodicum Biologorum Pub Date : 2021-07-01 DOI: 10.18054/pb.v123i1.6850

Mahassen Khalil., H. Elhariry, Tahany M. Alzaidi

{"title":"Disinfectant as Removal Agent of the Pre-Formed Biofilm by Staphylococcus sp. Isolated from Dental Clinics in Taif, KSA","authors":"Mahassen Khalil., H. Elhariry, Tahany M. Alzaidi","doi":"10.18054/pb.v123i1.6850","DOIUrl":"https://doi.org/10.18054/pb.v123i1.6850","url":null,"abstract":"Background and purpose: Staphylococcus sp. are pathogenic bacteria widely investigated for its high incidence in clinical environments and its ability to form strong biofilms. The biofilm-related infections can resist several antimicrobial agents; therefore, the therapy of these infections may face difficulties. The aim of this study is to identify staphylococcal bacteria isolated from dental clinics, evaluate the effect of some disinfectants on removing the preformed biofilms and to demonstrate the adhesion of cells on the surfaces of some materials used for manufacturing the dental material using Scanning Electron Microscope (SEM). Material and Methods: Out of 70 different swabs, 12 Staphylococcal isolates were recovered. All isolates were subjected to 13 antimicrobial agents to test their sensitivity. The biofilm established isolates, as well as the effect of some disinfectant as removal agents of the preformed biofilm were estimated using microtiter plate (MtP) test. SEM was used for demonstrating biofilm formation by the tested isolates on stainless steel and rubber surfaces. Results: Of 12 Staphylococcus isolates, 33.3, 16.6 and 50% were shown strong, moderate and weak biofilm producers, respectively. Adhesions of the preformed biofilm were found to be reduced in the presence of betadine and sodium bicarbonate. Captured photographs by SEM confirmed the capability of biofilm formation by S. aureus H3 and S. hominis M0401 on stainless steel and rubber surfaces. Conclusion: the effective preventive strategies for infection control are essential to prevent staphylococcal infections in the dental clinics. This study reveals effective antibiofilm agents as betadine and sodium bicarbonate.","PeriodicalId":19950,"journal":{"name":"Periodicum Biologorum","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49620933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A remarkable teratological case in Carabus violaceus L. (Coleoptera: Carabidae) from Poland 波兰紫背锦鸡畸形一例（鞘翅目：锦鸡科）

IF 0.3 4区生物学

Periodicum Biologorum Pub Date : 2021-07-01 DOI: 10.18054/pb.v123i1.7790

A. Schwerk, D. Klich, E. Wojtowicz

引用次数: 0

A multi-level system for planning compensatory habitats as a new tool to prevent biodiversity loss in protected areas due to development plans 一个多层次的补偿栖息地规划系统，作为防止保护区因发展计划而丧失生物多样性的新工具

IF 0.3 4区生物学

Periodicum Biologorum Pub Date : 2021-07-01 DOI: 10.18054/pb.v123i1.7161

Renata Rozman, D. Tome, M. Debeljak

{"title":"A multi-level system for planning compensatory habitats as a new tool to prevent biodiversity loss in protected areas due to development plans","authors":"Renata Rozman, D. Tome, M. Debeljak","doi":"10.18054/pb.v123i1.7161","DOIUrl":"https://doi.org/10.18054/pb.v123i1.7161","url":null,"abstract":"Background \u0000In Slovenia, compensatory habitats (CH) are currently determined on the basis of a subjective expert judgement and without using any clearly defined methodology, due to which the success rates of CH implementation are low. The aim of this research is to fill in a methodological gap and propose a new multi-level system for planning CH. The system assures a transparent and more objective determination of the size of a CH in the processes of appropriate assessments (AA). \u0000 Materials and methods: \u0000The system was developed by using a multi criteria decision analysis, a multi-attribute decision support model and the DEXi modelling tool. It was tested on a study case the Škofljica bypass road with its impact to a Whinchat (Saxicola rubetra) at Natura 2000 site Ljubljansko barje. \u0000 Results: \u0000The system with three modules and a possibility of what-if analysis was developed to assess the species endangerment and the size of the CH. The system identified that the case study has significant impacts to the Whinchat, therefore the CH of a slightly larger size than the habitat lost was proposed. In addition, the system indicated that only one of the three potential locations of the CH is suitable for implementing the CH. \u0000 Conclusions: \u0000The system allows a transparent and more objective assessment of the spatial plan. It is a new, easy-to-use, adjustable, cost- and time-efficient method that can be used to make reliable and transparent decisions during the assessment processes. \u0000 Keywords: \u0000Natura 2000; Offsetting; Ecological compensation; Habitat (re)creation; Appropriate Assessment; Sustainable development;","PeriodicalId":19950,"journal":{"name":"Periodicum Biologorum","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45373973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of microbiological indoor air quality in a public hospital in the city of Agadir, Morocco 摩洛哥阿加迪尔市一家公立医院室内微生物空气质量评估

IF 0.3 4区生物学

Periodicum Biologorum Pub Date : 2021-07-01 DOI: 10.18054/pb.v123i1.6461

Said Oulkheir, Halima Safouan, Fadwa El Housse, M. Aghrouch, K. Ounine, A. Douira, S. Chadli

{"title":"Assessment of microbiological indoor air quality in a public hospital in the city of Agadir, Morocco","authors":"Said Oulkheir, Halima Safouan, Fadwa El Housse, M. Aghrouch, K. Ounine, A. Douira, S. Chadli","doi":"10.18054/pb.v123i1.6461","DOIUrl":"https://doi.org/10.18054/pb.v123i1.6461","url":null,"abstract":"Background and Purpose: Air is the greatest dissemination agency of pathogenic microbes, which cause significant problem in the indoor hospital environment, in particularly in terms of nosocomial infections. In this context, it is important to know the types of microorganisms present in suspension in an air sample to assess the initial situation and the effectiveness of corrective measures. \u0000 Materials and Methods: This study aimed to assessment of microbiological indoor air quality in a three hospital units: central resuscitation, neonatal resuscitation and operating room, using a passive sampling method. \u0000 Results: Findings of this study indicated that the central resuscitation recorded the highest bacterial counts population 3.33 102 CFU/m3. Total viable count of fungal was recorded high in neonatal resuscitation. This research showed that Staphylococcus non aureus and Staphylococcus aureus were the most predominant among isolated bacteria. The percentage was 70% -21% in central resuscitation and 79%-13% in operating room. In neonatal resuscitation Staphylococcus non aureus represent (29%) followed by Staphylococcus aureus (19%), Pseudomonas aeruginosa (17%) and Pasteurella pneumotropica (16%). Thus, the fungal genera identified in operating room were Penicillium 61%, Aspergillus niger (20%) and Candida glabrata (19%). In neonatal resuscitation, we found Penicillium 51%, Candida glabrata 25% and Aspergillus niger 20%. In central resuscitation, the most predominant fungi were Cladosporium (30%), Penicillium (28%) and Candida glabrata (13%). \u0000 Conclusions: Microorganisms isolated from indoor air constitute microbial reservoirs that may present a risk of infection for both patients and staff. In this light microbiological monitoring of the environment in health facilities is a topic that is part of the news of the prevention of nosocomial infections.","PeriodicalId":19950,"journal":{"name":"Periodicum Biologorum","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41529236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First record of Pistia stratiotes L. (Araceae) in Croatia, with the consideration of possible introduction pathways 克罗地亚首个天南星科植物的记录，并考虑可能的引种途径

IF 0.3 4区生物学

Periodicum Biologorum Pub Date : 2021-07-01 DOI: 10.18054/pb.v123i1.7674

I. Boršić, Tomica Rubinić

{"title":"First record of Pistia stratiotes L. (Araceae) in Croatia, with the consideration of possible introduction pathways","authors":"I. Boršić, Tomica Rubinić","doi":"10.18054/pb.v123i1.7674","DOIUrl":"https://doi.org/10.18054/pb.v123i1.7674","url":null,"abstract":"Background and Purpose: The species Pistia stratiotes L. (Araceae) is South American (or pan-tropical) free-floating, aquatic macrophyte, present on all continents, except Antarctica, today. In Europe it occurs occasionally and casually in many countries, but it is established only in thermally abnormal waters in Slovenia, France and Germany. Although it was found in different European countries, even in Slovenia, it has never been recorded in Croatia. \u0000Materials and Methods: Floristic research in the Sava-Strmec Special reserve (Zagreb County) was carried out during autumn (September and November) of 2017, using GPS Receiver to determine investigated localities. \u0000Results: The species Pistia stratiotes was firstly recorded on 9th September 2017 on one locality in the Sava-Strmec Special reserve (Zagreb County). On 24th and 27th November 2017 several other localities in the vicinity were found. On each locality a small colony of plants was found. Although exact introduction pathway is not known, several possible pathways were taken into consideration and discussed. \u0000Conclusion: A first record of the macrophyte Pistia stratiotes for Croatia is reported here. For now, it should be treated as a casual alien species.","PeriodicalId":19950,"journal":{"name":"Periodicum Biologorum","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47322453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Evaluation of the Ultrastructure and Expression of Desmoglein 2 in Breast Cancer: A Novel Biomarker 一种新的生物标志物:乳腺癌中纤粒蛋白2的超微结构和表达的评价

IF 0.3 4区生物学

Periodicum Biologorum Pub Date : 2021-05-24 DOI: 10.21203/RS.3.RS-506346/V1

Maryam Mohammadhosseini, H. Mirzaei, A. Majd, M. Farhadi, N. Shayanfar

{"title":"Evaluation of the Ultrastructure and Expression of Desmoglein 2 in Breast Cancer: A Novel Biomarker","authors":"Maryam Mohammadhosseini, H. Mirzaei, A. Majd, M. Farhadi, N. Shayanfar","doi":"10.21203/RS.3.RS-506346/V1","DOIUrl":"https://doi.org/10.21203/RS.3.RS-506346/V1","url":null,"abstract":"\u0000 BackgroundBreast cancer is the most common malignancy among Iranian women. In recent years, the study of dysfunction in the expression of cell-cell junction genes and the related proteins in the malignant process has been at the center of attention. MethodsIn this study, 50 patients were selected who had both cancerous tissue and adjacent healthy tissue. The expression of the desmoglein 2 gene was evaluated. Healthy and cancerous tissue were compared using routine hematoxylin and eosin staining. The total protein was also compared between these two groups. The ultrastructural examination was performed.ResultsThe real-time polymerase chain reaction results showed a decrease in the expression of the desmoglein 2 gene in all tumor samples compared to the healthy samples (p-value <0.0001). Besides, receiver operating characteristic curve analysis showed that the area under the curve was equal to 0.98. Transmission electron microscopy microscopic studies revealed a change in the status of desmosomal junctions. These findings were consistent with the qualitative decrease in the protein expression between the two target groups.ConclusionOverall, the findings showed that the association between desmoglein 2 gene expression and alterations in cellular connections leads to impaired cellular connections, which is an important risk factor for breast cancer. This result proposed the understudy gene as a new biomarker in the development of breast cancer.","PeriodicalId":19950,"journal":{"name":"Periodicum Biologorum","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2021-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41311612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epigenetic drug 5-azacytidine impairs the potential for odontogenesis but improves tooth morphogenesis in the transplanted embryonic mandible 表观遗传药物5-氮杂胞苷损害了移植胚胎下颌骨的牙发生潜力，但改善了牙齿的形态发生

IF 0.3 4区生物学

Periodicum Biologorum Pub Date : 2020-12-30 DOI: 10.18054/PB.V121-122I1-2.10718

Vedran Radujković

引用次数: 1

Synthesis and hydrolysis of auxins and their conjugates with different side-chain lengths: are all products active auxins? 不同侧链长度的生长素及其偶联物的合成和水解：所有产物都是活性生长素吗？

IF 0.3 4区生物学

Periodicum Biologorum Pub Date : 2020-12-30 DOI: 10.18054/PB.V121-122I3-4.10516

J. Ludwig-Müller

{"title":"Synthesis and hydrolysis of auxins and their conjugates with different side-chain lengths: are all products active auxins?","authors":"J. Ludwig-Müller","doi":"10.18054/PB.V121-122I3-4.10516","DOIUrl":"https://doi.org/10.18054/PB.V121-122I3-4.10516","url":null,"abstract":"Plants need hormone substances to regulate a plethora of responses during their life cycle. One major hormone class is called auxin, which is involved in many developmental processes. Besides the major auxin indole-3-acetic acid, there are other auxin-like molecules present in some, but not in all plants, an example would be chlorinated IAA in legumes. Among these are also the auxins with longer chains, indole-3-propionic acid and indole3-butyric acid. The auxin-dependent growth response is dependent on the concentration of the compound. While lower concentrations are mainly growth promoting, high concentrations are actually inhibiting some developmental processes. Therefore, tight control of the auxin concentration is essential for proper growth and development. This can be achieved by altering the amount of active auxin via transport, biosynthesis, degradation or reversible conjugation to small molecules. In addition, plants use auxin during their interaction with the environment, for example during abiotic stresses such as salt, temperature or water stress to adapt the growth responses specifically. Furthermore, auxin is involved in the development of plant disease symptoms, such as tumor growth or aberrant tissue formation. However, together with other plant hormones such as salicylic acid auxin can also modulate disease progression or resistance in different plant – microbe combinations.","PeriodicalId":19950,"journal":{"name":"Periodicum Biologorum","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45777397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

CRISPR-Cas in Escherichia coli: regulation by H-NS, LeuO and temperature 大肠杆菌CRISPR-Cas：H-NS、LeuO和温度的调节

IF 0.3 4区生物学

Periodicum Biologorum Pub Date : 2020-12-30 DOI: 10.18054/PB.V121-122I3-4.10994

Dora Markulin

引用次数: 1