Pertanika Journal of Tropical Agricultural Science最新文献_第5页

The Effect of Different Lignocellulose Biomass-Based Substrates on the Enhancement of Growth, Yield, and Nutritional Composition of Grey Oyster Mushrooms 不同木质纤维素生物质基质对灰牡蛎生长、产量和营养成分的影响

IF 0.6

Pertanika Journal of Tropical Agricultural Science Pub Date : 2023-06-09 DOI: 10.47836/pjtas.46.3.04

N. H. Saidan, Mohd Shahrul Ridzuan Hamil, Khomaizon Abdul Kadir Pahirul Zaman, N. N. A. Zakaria, Norsyafiera Syuhada Fadzil, Kharul Azmi Muazzam Abdul Rahman

{"title":"The Effect of Different Lignocellulose Biomass-Based Substrates on the Enhancement of Growth, Yield, and Nutritional Composition of Grey Oyster Mushrooms","authors":"N. H. Saidan, Mohd Shahrul Ridzuan Hamil, Khomaizon Abdul Kadir Pahirul Zaman, N. N. A. Zakaria, Norsyafiera Syuhada Fadzil, Kharul Azmi Muazzam Abdul Rahman","doi":"10.47836/pjtas.46.3.04","DOIUrl":"https://doi.org/10.47836/pjtas.46.3.04","url":null,"abstract":"The local agricultural industry has produced a lot of biomass waste from agro-based materials, which contain much lignocellulose that can be used as substrates for oyster mushrooms (Pleurotus ostreatus) cultivation. This study aims to compare the effect of different lignocellulose biomass-based substrates on oyster mushrooms’ growth, yield, and nutritional composition. Three different substrates (cassava peel, sugarcane bagasse, and sawdust) were prepared, and the growth response was observed and measured. The fastest mycelial colonization was achieved from sugarcane bagasse after 29 days of cultivation. Oyster mushrooms grown with sugarcane bagasse substrate showed the highest carbohydrate, fiber, and energy content with 10.70%, 7.70%, and 52.00 kcal, respectively, compared to mushrooms grown with other substrates. The biological efficiency of sugarcane bagasse is also comparable to sawdust. Thus, it can be concluded that sugarcane bagasse has a high potential to be used as an alternative biomass-based substrate for cultivating P. ostreatus with enhancement on the growth, yield, and nutritional composition.","PeriodicalId":19890,"journal":{"name":"Pertanika Journal of Tropical Agricultural Science","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46222459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evaluation of Avian Papillomavirus Occurrences and Effective Sampling Materials for Screening Purpose in Bird Species Through Systematic Review and Meta-Analysis 通过系统回顾和荟萃分析评估禽乳头瘤病毒在鸟类中的发生率和用于筛查的有效采样材料

IF 0.6

Pertanika Journal of Tropical Agricultural Science Pub Date : 2023-05-16 DOI: 10.47836/pjtas.46.2.17

Nur Najihah, A. Nurul Najian, A. Syahir, J. Abu, K. L. Ho, Wen Siang Tan, A. R. Mariatulqabtiah

{"title":"Evaluation of Avian Papillomavirus Occurrences and Effective Sampling Materials for Screening Purpose in Bird Species Through Systematic Review and Meta-Analysis","authors":"Nur Najihah, A. Nurul Najian, A. Syahir, J. Abu, K. L. Ho, Wen Siang Tan, A. R. Mariatulqabtiah","doi":"10.47836/pjtas.46.2.17","DOIUrl":"https://doi.org/10.47836/pjtas.46.2.17","url":null,"abstract":"Papillomaviruses (PVs), double-stranded circular DNA viruses, typically cause regressing papillomas (warts) on mucosal or keratinized epithelia of a wide spectrum of species. The viruses largely infect mammals, whereby PV infections in humans, bovines, and rabbits are extensively reported. However, studies on non-mammalian PVs, particularly avian ones, are relatively lacking and worthy of investigation. This study performed a meta-analysis post-systematic review based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses statement guidelines to evaluate the occurrences of avian papillomaviruses (APVs) in bird species and effective materials used for virus detection. The electronic databases Science Direct, Medline via PubMed, and Google Scholar were used to search for the journal articles. Upon article eligibility check, the QUADAS-2 was employed to assess the data. Of 1139 records, 31 were eligible for full-text review, but only 9 were significant for the final review. The results showed that APVs are highly prevalent among the Fringillidae family, with a proportion of 81%, followed by Laridae (30%) and Anatidae (13%). The pooled prevalence of APV in tissue samples was 38%, while in swab samples was 13%. Only one study reported positive APV from fecal materials (0.4%); hence, the reliability comparison between these three samples was not performed. This study concluded that APVs are most prevalent in the Fringillidae bird family, while tissues are the most suitable biological samples for APV screening and should be considered as a single sample material. From epidemiology, knowledge of APV incidences and distribution may assist in controlling papillomatosis in bird species.","PeriodicalId":19890,"journal":{"name":"Pertanika Journal of Tropical Agricultural Science","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49163507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Study on Leaf Anatomy in Selected Garcinia Species in Peninsular Malaysia 马来西亚半岛藤黄属植物叶片解剖的比较研究

IF 0.6

Pertanika Journal of Tropical Agricultural Science Pub Date : 2023-05-16 DOI: 10.47836/pjtas.46.2.18

Aiesyaa Majdiena Emlee, Che Nurul Aini CHE AMRI, Mohd Razik Midin

{"title":"Comparative Study on Leaf Anatomy in Selected Garcinia Species in Peninsular Malaysia","authors":"Aiesyaa Majdiena Emlee, Che Nurul Aini CHE AMRI, Mohd Razik Midin","doi":"10.47836/pjtas.46.2.18","DOIUrl":"https://doi.org/10.47836/pjtas.46.2.18","url":null,"abstract":"A comparative study of leaf anatomy was attempted on Garcinia species in Peninsular Malaysia to identify anatomical features useful in species identification and classification. The species are Garcinia mangostana var. mangostana , Garcinia mangostana var. malaccensis , and Garcinia celebica. Leaves were collected from two different regions: Kuantan, Pahang and Kepong, Kuala Lumpur. The leaf anatomical study was done using the methods of leaf peeling, leaf venation, leaf cross-section, and scanning electron microscopy. The assessment of the leaf anatomy found that these three Garcinia species showed similarities in anatomical features, including the presence of paracytic stomata on the abaxial surface, a straight to wavy anticlinal wall of both adaxial and abaxial surfaces, a thick cuticle wax layer, the presence of druses, mucilage canal, petiole vascular bundle, the presence of collenchyma cells in the midrib, and also the presence of sclerenchyma cells in midrib and petiole. Meanwhile, the notable anatomical variation observed in this study included three types of midrib vascular bundles: the outline of the leaf margin, the presence of tanniferous idioblast, leaf marginal, and laminal venation as six types of epicuticular waxes present on epidermal surface. Overall, this study highlighted the anatomical features that are taxonomically valuable, which could be used to identify selected Garcinia species in Malaysia.","PeriodicalId":19890,"journal":{"name":"Pertanika Journal of Tropical Agricultural Science","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49483020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Phytase Enzyme Improves Growth Performance and Body Chemical Composition of Sangkuriang Catfish (Clarias gariepinus var. Sangkuriang) Juvenile 植酸酶对桑库良鲶鱼幼鱼生长性能和体化学成分的影响

IF 0.6

Pertanika Journal of Tropical Agricultural Science Pub Date : 2023-05-16 DOI: 10.47836/pjtas.46.2.20

D. Rachmawati, P. H. Riyadi, I. Samidjan, T. Elfitasari, Diana Chilamawati, S. Windarto, Rosa Amalia, Dewi Nurhayati, Tristiana Yuniarti, Yunanto Yunanto

{"title":"Phytase Enzyme Improves Growth Performance and Body Chemical Composition of Sangkuriang Catfish (Clarias gariepinus var. Sangkuriang) Juvenile","authors":"D. Rachmawati, P. H. Riyadi, I. Samidjan, T. Elfitasari, Diana Chilamawati, S. Windarto, Rosa Amalia, Dewi Nurhayati, Tristiana Yuniarti, Yunanto Yunanto","doi":"10.47836/pjtas.46.2.20","DOIUrl":"https://doi.org/10.47836/pjtas.46.2.20","url":null,"abstract":"Due to their nutritional value, alternative vegetable materials such as soybean are needed to promote sustainable aquaculture development. However, phytate in soybean could interfere with the protein digestion of the fish body. This study examines the effectiveness of phytate enzymes in enhancing protein digestibility, growth performance, mineral digestion, and body chemical composition of Sangkuriang catfish (Clarias gariepinus var. Sangkuriang) juveniles. The study was completely randomized design with 4 treatments and 3 repetitions. One hundred thirteen Sangkuriang catfish juveniles (7.65 ± 0.14 g) were used for each repetition. The fish were fed with an experimental diet supplemented with various doses of phytase enzyme: 0 (A), 500 (B), 1,000 (C), and 1,500 (D) FTU/kg of feed. The mineral digestibility, protein digestibility, protein efficiency ratio (PER), feed conversion ratio (FCR), the efficiency of feed utilization (EFU), apparent digestibility coefficients (ADCp), relative growth rate (RGR), and survival rate (SR) were observed. Data were analyzed using analysis of variance followed by Duncan’s multiple range test. The results showed that adding 1,000 FTU/kg of phytase enzyme (C) exhibited the highest PER, FCR, EFU, ADCp, and RGR value of Sangkuriang catfish juveniles compared to other treatments. All treatment groups (B-C) had the same SR value (100%) and had the highest crude protein and ash content compared to the A group. All group treatments also exhibited a higher phosphorus, calcium, potassium, magnesium, iron, zinc, manganese, copper, and cobalt. Therefore, phytase enzyme supplementation could improve protein digestibility, mineral digestibility, growth performance, and body chemical composition of Sangkuriang catfish juveniles.","PeriodicalId":19890,"journal":{"name":"Pertanika Journal of Tropical Agricultural Science","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44200353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Edible Coatings (Spirulina and Chitosan) on the Quality and Shelf Life of Starfruit (Averrhoa carambola L. cv. B10) Throughout Storage 食用包衣(螺旋藻和壳聚糖)对杨桃品质和保质期的影响。B10)整个存储

IF 0.6

Pertanika Journal of Tropical Agricultural Science Pub Date : 2023-05-16 DOI: 10.47836/pjtas.46.2.19

Zahrah-Izati Azhar Shapawi, S. H. Ariffin, R. Shamsudin, Muhammad Syahmeer How, Ahmad Hafiz Baharom

{"title":"The Effect of Edible Coatings (Spirulina and Chitosan) on the Quality and Shelf Life of Starfruit (Averrhoa carambola L. cv. B10) Throughout Storage","authors":"Zahrah-Izati Azhar Shapawi, S. H. Ariffin, R. Shamsudin, Muhammad Syahmeer How, Ahmad Hafiz Baharom","doi":"10.47836/pjtas.46.2.19","DOIUrl":"https://doi.org/10.47836/pjtas.46.2.19","url":null,"abstract":"The edible coating is one of the preservation methods widely applied by food industries as it is beneficial in suppressing respiration, minimising moisture loss, and reducing food wastage. This study investigates the effects of edible coating (Spirulina platensis and chitosan) on the quality and shelf life of B10 throughout storage at room temperature 27°C. The quality analysis of colour (L*, a*, b*, and hue), browning index, fresh weight and physical appearance were evaluated on days 0, 4, 8, 12, and 14. There was a significant difference for all quality analyses between storage days (p < 0.05). The physical appearance showed that at day 8, B10 coated with spirulina maintained the greenish colour while chitosan-coated and controlled turned the fruit bright yellowish, indicating ripening. Control samples were observed to have major browning at day 12, whereas samples coated with spirulina and chitosan only showed early signs of browning. Samples with spirulina coating have the least a* (greenness-redness) and b* (blueness-yellowness) values, which showed that spirulina was able to slow down the ripening process in comparison to chitosan and control (p < 0.05). For the hue value, samples with chitosan coating showed the least colour changes (p < 0.05), followed by spirulina and control with 9.04, 9.43, and 30.82°, respectively. It proved that coated samples provide the best results in slowing down the colour changes and browning of the carambola compared to the control. Besides, the regression analysis resulted in a good fitness (R2 near 1) for browning and weight loss analysis for all coatings, which were agreed to be reliable and had a good predictive indicator power when the storage days were extended. Hence, these results would be potentially useful for the fresh produce industry to prolong the shelf-life of B10 during distribution, transportation, and commercialisation.","PeriodicalId":19890,"journal":{"name":"Pertanika Journal of Tropical Agricultural Science","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42014593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Salicylic Acid and Gibberellic Acid Increase Stem Cutting Growth of Pennisetum purpureum cv. Mahasarakham and Pennisetum purpureum x Pennisetum americanum 水杨酸和赤霉酸的应用促进了马哈萨拉姆狼尾草和美国狼尾草的茎部生长

IF 0.6

Pertanika Journal of Tropical Agricultural Science Pub Date : 2023-05-05 DOI: 10.47836/pjtas.46.3.01

Amnat Phetsuwan, Narisa Kunpratum, Marootpong Pooam, K. Somtrakoon, W. Chouychai

{"title":"Application of Salicylic Acid and Gibberellic Acid Increase Stem Cutting Growth of Pennisetum purpureum cv. Mahasarakham and Pennisetum purpureum x Pennisetum americanum","authors":"Amnat Phetsuwan, Narisa Kunpratum, Marootpong Pooam, K. Somtrakoon, W. Chouychai","doi":"10.47836/pjtas.46.3.01","DOIUrl":"https://doi.org/10.47836/pjtas.46.3.01","url":null,"abstract":"The Napier grass continues to be a popular forage crop in Nakhonsawan Province and other areas in the northern and east-northern parts of Thailand. Increasing the growth and biomass of Napier grass growing in low-quality sandy soil will increase the economic value of this plant. The stem cutting of two cultivars of Napier grass, cv. Pakchong 1 (Pennisetum purpureum x Pennisetum americanum) and sweet grass (Pennisetum purpureum cv. Mahasarakham), were exposed to two plant growth regulators, gibberellic acid and salicylic acid, and two application methods, soaking for 24 hr and watering after inoculation into the soil, were studied. For one plant growth regulator test, the most appropriate concentration of gibberellic acid and salicylic acid were 0.01 and 100 mg/L, respectively, for the soaking and watering method. There was an interaction between the combination of plant growth regulator type and application method for both cultivars. The best stimulation effect for sweet grass was soaking with 100 mg/L salicylic acids only. The response of Napier grass cv. Pakchong 1 was different. Soaking with 0.01 mg/L gibberellic acid and watering with 0.01 mg/L gibberellic acid was suitable for increasing shoot and root length while soaking with 0.01 mg/L gibberellic acid and watering with 100 mg/L salicylic acid was suitable for increasing fresh and dry weight. This information will be useful for stimulating the growth of Napier grass in agricultural purposes.","PeriodicalId":19890,"journal":{"name":"Pertanika Journal of Tropical Agricultural Science","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48498550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of Spacing on Early Growth Rate and Yield of Hybrid Eucalyptus Stands 间距对杂交桉树林分早期生长率和产量的影响

IF 0.6

Pertanika Journal of Tropical Agricultural Science Pub Date : 2023-04-18 DOI: 10.47836/pjtas.46.2.15

G. L. Fernandes, G. G. Casas, L. Fardin, Gilciano Saraiva Nogueira, R. Leite, L. Couto, H. Leite

{"title":"Effects of Spacing on Early Growth Rate and Yield of Hybrid Eucalyptus Stands","authors":"G. L. Fernandes, G. G. Casas, L. Fardin, Gilciano Saraiva Nogueira, R. Leite, L. Couto, H. Leite","doi":"10.47836/pjtas.46.2.15","DOIUrl":"https://doi.org/10.47836/pjtas.46.2.15","url":null,"abstract":"Optimizing tree spacing in a forest plantation is one of the main management techniques to improve stand quality and productivity. Its influence on growth from an early age is an important matter for forest management. This study aims to evaluate the effect of tree spacing on early growth rate and yield over time in Eucalyptus grandis × Eucalyptus camaldulensis hybrids. The data were obtained from an experiment in Itamarandiba, Minas Gerais, Brazil. The plots were composed of five planting spacing (3.00 m × 0.50 m, 3.00 m × 1.00 m, 3.00 m × 1.50 m, 3.00 m × 2.00 m, and 3.00 m × 3.00 m) measured at the ages of 7, 12, 24, 36, 48, 61, 77, 85, and 102 months. Growth and yield were analyzed by fitting the Gompertz model and a baseline exponential model up to 36 months of age to evaluate the influence of early growth on the harvest age. A Pearson correlation matrix was also generated to find out the relationship between the mean annual increment in the respective treatments during the studied period. It was observed that a positive correlation in the average annual increase in the 3 × 2 and 3 × 3 spacings. It was verified that tree spacing influenced the yielded wood volume and the optimal harvest age. The early growth rate influences the optimal harvest, which may explain a possible loss of yield during the productive cycle of the forest stand.","PeriodicalId":19890,"journal":{"name":"Pertanika Journal of Tropical Agricultural Science","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42419154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Revisiting In vitro Micropropagation Protocols of Mimosa pudica for Enhanced Seed Germination, Shoot Multiplication, and Root Initiation 重述含羞草体外微繁技术促进种子萌发、芽增殖和根形成

IF 0.6

Pertanika Journal of Tropical Agricultural Science Pub Date : 2023-04-18 DOI: 10.47836/pjtas.46.2.12

Norshidah Baharuddin, Muhamad Aidilfitri Mohamad Roslan, Noor Amani Ramli, Azzreena Mohamad Azzeme, Zuraida Ab Rahman, M. E. Khayat, Helmi Wasoh, Zulfazli M. Sobri

{"title":"Revisiting In vitro Micropropagation Protocols of Mimosa pudica for Enhanced Seed Germination, Shoot Multiplication, and Root Initiation","authors":"Norshidah Baharuddin, Muhamad Aidilfitri Mohamad Roslan, Noor Amani Ramli, Azzreena Mohamad Azzeme, Zuraida Ab Rahman, M. E. Khayat, Helmi Wasoh, Zulfazli M. Sobri","doi":"10.47836/pjtas.46.2.12","DOIUrl":"https://doi.org/10.47836/pjtas.46.2.12","url":null,"abstract":"Mimosa pudica is a medicinal plant worthy of therapeutic properties. It is often overlooked as one of the weed species, and the potential was underappreciated despite its abundance in nature, particularly in tropical climate countries. Considering the aptitude of this species, the micropropagation protocol of M. pudica was revisited and enhanced. The seed surface sterilization and germination were assessed, followed by shoot multiplication rate and root initiation efficiency. Seeds of M. pudica were best surface sterilized with 35% of Clorox and recorded the highest germination rate at 65.55% in media of three-quarter strength Murashige and Skoog (MS) combined with 30 g of sucrose. The explants were harvested and subcultured into multiplication media fortified with 6-benzylaminopurine, kinetin, and coconut water. The highest number of new shoots and leaves were obtained at 3 shoots and 3.27 cm in media of MS combined with 17.6 μm kinetin. Half-strength MS media devoid of hormone was recommended for root induction, resulting in the 5 healthy roots at 2.06 cm after 4 weeks of culture. Overall, the developed micropropagation protocol of M. pudica could facilitate its large-scale cultivation, indicating its potential as a medicinal crop for the extraction of bioactive compounds.","PeriodicalId":19890,"journal":{"name":"Pertanika Journal of Tropical Agricultural Science","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70929308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of Proteolytic Enzyme from Pineapple Crown 菠萝冠蛋白水解酶的分离

IF 0.6

Pertanika Journal of Tropical Agricultural Science Pub Date : 2023-04-18 DOI: 10.47836/pjtas.46.2.14

Noorazizah Arsad, Azman Abd Samad, H. Jamaluddin, W. R. Z. Wan Dagang

{"title":"Isolation of Proteolytic Enzyme from Pineapple Crown","authors":"Noorazizah Arsad, Azman Abd Samad, H. Jamaluddin, W. R. Z. Wan Dagang","doi":"10.47836/pjtas.46.2.14","DOIUrl":"https://doi.org/10.47836/pjtas.46.2.14","url":null,"abstract":"The pineapple waste from the pineapple industry has contributed to an increase in waste in Malaysia and worldwide every year. A major type of endopeptidase enzymes found in pineapple is fruit bromelain, stem bromelain, ananain, and comasain. This study aims to extract and purify protease from the crown of MD2 pineapple. Protease was extracted and purified using anion exchange chromatography, gel filtration, and desalting before being identified using liquid chromatography-mass spectrometry (LC-MS). Proteolytic activity was determined using the well diffusion method and Casein Digestion Unit. In the present study, the proteolytic assay showed that 1 kg crown of MD2 cultivar produced an activity of 126.0 ± 3.86 U/ml, a specific activity of 3937.50 U/mg. In the present study, the proteolytic assay showed that 1 kg crown of MD2 cultivar produced an activity of 126.0 ± 3.86 U/mL, a specific activity of 3937.50 U/mg and the total activity of 3.94 × 109 U. The molecular weight of the purified enzyme was in the range of 25 to 35 kDa under the optimum condition of pH 7 and 37°C. Purification of the extract yielded a band at the molecular weight of 20–25 kDa at the optimum pH of 3 and 9 at 60°C. From LC-MS analysis, the purified enzyme from the crown extract was similar to ananain under accession number A0A199VSS3 (according to Uniprot). It had five unique peptides and covered 97/356 amino acids (44.9% coverage). The ananain (EC 3.4.22.31) is classified in the subfamilies of cysteine protease C1A (clan CA, family C1), a peptidase family related to papain. In conclusion, protease was extracted and identified as an ananain-like protease from the crown.","PeriodicalId":19890,"journal":{"name":"Pertanika Journal of Tropical Agricultural Science","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44315958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Parthenium hysterophorus Weed Fecundity and Seed Survival at Different Soil pH and Burial Conditions 不同土壤pH值和埋置条件下独雌菊的杂草繁殖力和种子存活率

IF 0.6

Pertanika Journal of Tropical Agricultural Science Pub Date : 2023-04-18 DOI: 10.47836/pjtas.46.2.13

M. Ghazali, U. Sinniah, M. S. Ahmad-Hamdani

{"title":"Parthenium hysterophorus Weed Fecundity and Seed Survival at Different Soil pH and Burial Conditions","authors":"M. Ghazali, U. Sinniah, M. S. Ahmad-Hamdani","doi":"10.47836/pjtas.46.2.13","DOIUrl":"https://doi.org/10.47836/pjtas.46.2.13","url":null,"abstract":"Parthenium hysterophorus L. is regarded as one of the most invasive weed species. This study evaluated the effect of soil pH on P. hyterophorus weed growth and fecundity, as well as the effect of burial depths on P. hyterophorus seed survival and emergence. The first study evaluated five soil pH levels (acidic, sub-acidic, neutral, sub-basic, and basic) in a randomised complete block design. Seed germinability and subsequent seedling growth (height, leaf area, biomass, and seed number/plant) were evaluated. This study showed that P. hysterophorus seeds have a similar germination capacity under varying soil pH conditions. However, acidic soil indicated accelerated growth (25% higher biomass with 15.2% more leaf area) and fecundity (13.4% faster to enter the flowering stage at 74 days after sowing). In the second study, two factors were tested; burial depths (0.5, 5, 10, and 20 cm) being nested into seven burial durations (0, 2, 4, 6, 8, 10, and 12 months). Seed viability (final germination percentage and germination rate index), electrical conductance, and emergence percentage were assessed with the burial conditions. The results showed that only seed buried at 0.5 cm depth indicated emergence (81.3% of cumulative emergence). Forty per cent of the seeds remained viable after exhumation at 0.5–5 cm depth, while only 19–27% survived as the burial depth increased beyond 10 cm depth within 12 months. The seed remained viable for more than 12 months as a seed bank, despite faster deterioration at increasing soil depth.","PeriodicalId":19890,"journal":{"name":"Pertanika Journal of Tropical Agricultural Science","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43572766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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