{"title":"Influence of Trichoderma harzianum- seed Coating on the Biochemical Characteristics of Wheat (Triticum aestivum L.) Under Salt Stress","authors":"Maleeha Umber, Rashida Sultana, Faiza Nasir, Rizwana Mubashar, Ragheeba Sehar","doi":"10.21743/pjaec/2021.12.08","DOIUrl":"https://doi.org/10.21743/pjaec/2021.12.08","url":null,"abstract":"Salt stress is one of the main limitations to Triticum aestivum productivity all around the world. An experiment was conducted to assess the effect of Trichoderma harzianum seed coating on germination and seedling development of wheat under salt stress (60 and 120 mM NaCl). The seeds of six wheat cultivars, namely Shafaq-06, Punjab-11, Millet, Seher, Pirsabik, and Aari were seed coated with Trichoderma (taken from NARC Pakistan) at the rate of 2 x 107 CFU using PelGel for 24 h. After air-drying at room temperature for 12 h, the coated seeds were sown in small pots. Experimentation was laid out in a completely randomized design with three replications. The data for various biochemical attributes were collected after 30 d of germination to test the seed and seedling vigor, respectively. Trichoderma harzianum seed coating reduced the amount of hydrogen peroxide, catalase, Malondialdehyde, and increased protein content, Ascorbate Peroidase, and total phenolics under salt stress advocating that its use is effective in the cultivation of crops in saline areas because it inhibits oxidative damage by triggering various phenolic compounds and scavenging proteins.","PeriodicalId":19846,"journal":{"name":"Pakistan Journal of Analytical & Environmental Chemistry","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47893428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hizbullah Khan, Muhammad Najeeb Khan, M. Sirajuddin, S. Salman, M. Bilal
{"title":"Assessment of Drinking Water Quality of Different Areas in Tehsil Isa Khel, Mianwali, Punjab, Pakistan","authors":"Hizbullah Khan, Muhammad Najeeb Khan, M. Sirajuddin, S. Salman, M. Bilal","doi":"10.21743/pjaec/2021.12.16","DOIUrl":"https://doi.org/10.21743/pjaec/2021.12.16","url":null,"abstract":"Water samples were collected from 43 sites of Tehsil Isa Khel areas in order to determine the physicochemical parameters such as pH, electrical conductivity, turbidity, total hardness, calcium hardness, magnesium hardness, M. alkalinity, chloride ion (Cl- ), and fluoride ion (F-) concentration. The obtained results show that in Tehsil Isa Khel, only in Kala Bagh city, Kala Bagh water scheme (w/s), Tola Bangi Khel w/s, Kot Chandna, Awan Wala, Gidran Wala, and Cheena Pora water is drinkable. Overall, electrical conductivity, hardness, total alkalinity, chloride, fluoride levels in the water of Tehsil Isa Khel are very high and not fit for drinking, washing, and industrial purpose. The ultimate result of this study is helpful to address the leading cause of public health problems related to the deteriorated quality of drinking water, and an integrated approach is therefore required to provide safe drinking water to people in Tehsil Isa Khel.","PeriodicalId":19846,"journal":{"name":"Pakistan Journal of Analytical & Environmental Chemistry","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42249662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Carbon Based Electrode Materials and their Architectures for Capacitive Deionization","authors":"B. Samejo, Shagufta Gul, Suraya Samejo, Naveed Qasim Abro, Najma Memon","doi":"10.21743/pjaec/2021.12.02","DOIUrl":"https://doi.org/10.21743/pjaec/2021.12.02","url":null,"abstract":"The effective desalination and purification devices for seawater/ brackish water treatment are crucial in sustainable progress. Techniques that render high salt removal efficiency and water purification ability at low applied potentials play a central role in sustainable water supplies. One of them is capacitive deionization (CDI) which has drawn significant consideration as a promising deionization technology since the last decade. Desalination efficiency profoundly depends on the utilized electrode material. The most widely used CDI electrodes are carbons due to their cost effectiveness and good stability. However, to acquire high electrosorption capacity, extensive researches are reported with modified carbon materials. CDI cell architectures are equally important for practical high salt removal performance. This review focuses on carbon materials in CDI along with other emerging trends in diverse carbon types, e.g., carbon nanotubes and their composites. Various architectures reported in the literature to improve desalination efficiency are also included here.","PeriodicalId":19846,"journal":{"name":"Pakistan Journal of Analytical & Environmental Chemistry","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45132127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Determination of Total Phenolic, Flavonoid Content and Antimicrobial Properties in Different Solvent Extracts of Viburnum opulus L. (Gilaburu) in Afyonkarahisar","authors":"M. Düz, S. E. Korcan, G. U. Akkuş","doi":"10.21743/pjaec/2021.12.17","DOIUrl":"https://doi.org/10.21743/pjaec/2021.12.17","url":null,"abstract":"This study determined the antimicrobial and antioxidant activities of chloroform, ethyl acetate, methanol, and water extracts of the Viburnum opulus (V. opulus) plant. V. opulus (Gilaburu) was collected from Ahırdağı northern slope, Kırka Town, Afyonkarahisar region, and its leaves, branches, and fruit parts were dried and powdered at room temperature. The total phenolic content was compared with the gallic acid standard, the total flavonoid content with the quercetin standard, and the iron chelating effect with the EDTA standard. All extracts were evaluated for antimicrobial activity against Escherichia coli, Staphyllococcus aureus, and Candida glabrata strains by the agar well diffusion method. Gilaburu is widely used in Turkey, especially in alternative medicine. It has been determined that the fruit part of the plant has better antimicrobial properties, and the amount of phenolic compounds increases in more polar solvents. In conclusion, the use of Gilaburu as a medicinal drug can be achieved with new research on bioactive substances, especially in fruit extract.","PeriodicalId":19846,"journal":{"name":"Pakistan Journal of Analytical & Environmental Chemistry","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45294157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. A. Munir, K. Badri, L. Heng, M. M. Mackeen, E. E. Sage
{"title":"Histamine Detection in Mackerel (Scomberomorus Sp.) and its Products Derivatized with 9-Flourenilmethylchloroformate","authors":"M. A. Munir, K. Badri, L. Heng, M. M. Mackeen, E. E. Sage","doi":"10.21743/pjaec/2021.12.03","DOIUrl":"https://doi.org/10.21743/pjaec/2021.12.03","url":null,"abstract":"Histamine is commonly present in food containing proteins, like in mackerel. Consuming fish is imperative for the improvement of human muscles. Nevertheless, some studies reported ingesting fish containing histamine more than 50 mg·kg-1 can cause toxicity. This study analyzed and determined the composition of histamine in mackerel and its products commonly consumed in Malaysia, especially on the East Coast of Malaysia. These included processed mackerel such as canned products, satay (skewed fish) and keropok lekor (fish cake/ cracker). Histamine analysis was performed using High Performance Liquid Chromatography (HPLC) equipped with a fluorescence detector. A derivatizing reaction was applied to increase the sensitivity of HPLC to histamine using 9-flourenilmethylchloroformate (FMOC-Cl). The chromatographic separation was achieved in 15 min. Method validation was in accordance to Commission Decision 657/2002/CE. The linear range was at 0.16 – 5.00 µg·mL-1 (histamine) with the LOD at 0.10 µg·mL-1 and LOQ at 0.30 µg·mL-1 . Method applicability was checked on seven real samples involving raw, cooked, and dry products, yielding acceptable recovery.","PeriodicalId":19846,"journal":{"name":"Pakistan Journal of Analytical & Environmental Chemistry","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45849313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Statistical Analysis of SO2 and NO2 with Respect to Meteorological Parameters (Gwalior, India)","authors":"Mehrajuddin Bhat, A. C. Pandey","doi":"10.21743/pjaec/2021.12.07","DOIUrl":"https://doi.org/10.21743/pjaec/2021.12.07","url":null,"abstract":"The present study is carried out in Gwalior to know the level of pollutants viz sulphur dioxide (SO2) and nitrogen dioxide (NO2). In this study, both NO2 and SO2 were collected during different seasons, and estimation was done using chemical methods. The methods used for the determination of SO2 and NO2 in the ambient air of Gwalior was (Modified West and Geake method) and (Modified Jacob and Hochheisier). The SO2 from the air stream was absorbed in a sodium tetramer curate solution. NO2 was collected by bubbling air through a sodium hydroxide solution to form a stable solution of sodium nitrate. Meteorological parameters like temperature, relative humidity were recorded by thermometers and hygrometry during the sampling. Rainfall data was taken from Indian Meteorological Department, New Delhi, for four sampling years. The statistical analysis was carried out between the level of pollutants SO2 and NO2 measured and meteorological parameters recorded during the sampling. This study observed that pollutants were very high in winter and summer compared to monsoon and post-monsoon periods due to the heavy transport movement and favourable meteorological conditions like temperature, humidity, rainfall, and wind speed and directions.","PeriodicalId":19846,"journal":{"name":"Pakistan Journal of Analytical & Environmental Chemistry","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48101060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Assessment of Cadmium Toxicity in Drinking Water of Eight Talukas of Upper Sindh","authors":"N. Shar, G. Q. Shar","doi":"10.21743/pjaec/2021.12.09","DOIUrl":"https://doi.org/10.21743/pjaec/2021.12.09","url":null,"abstract":"In the present study, 240 groundwater samples were collected from eight Talukas of upper Sindh. The cadmium (Cd) content was determined by atomic absorption spectrophotometer. Concentration (µg/L), Daily Intake ofMetals (DIM),Health Risk Indexes (HRI) for children and adults caused due to Cd were measured through equations of EPA and WHO. Mean concentration of Cd in different Talukas of upper Sindh was found as; Daharki (6.20 µg/L), Ubauro (7.73 µg/L), Kashmore (7.53 µg/L), Tangwani (7.73 µg/L), Garhi Khairo (6.20 µg/L), Thul (5.00 µg/L), Qambar (7.674 µg/L) and Miro Khan (7.47 µg/L). Moreover, percent contamination of each Taluka was found as, Daharki (80%), Ubauro (93%), Kashmore (93%), Tangwani (93%), Gharhi Khairo (70%), Thul (56%), Qambar (93%) and Miro Khan (93%). The DIM of Cd for adult and children was calculated the range for adults was found as; Daharki (0.00006 - 0.00036), Ubauro (0.00006 - 0.00042), Kashmore (0.00006 - 0.00036), Tangwani (0.00006 - 0.00042), Garhi Khairo (0.00003 - 0.00056), Thul (0.00003 - 0.00056), Qambar (0.00008 - 0.00039) and Miro Khan (0.00008 - 0.00039) mg/kg-d, whereas, for children DIM of Cd was found lower than adults. The HRI values show that children compared to adults are under the potential risk threat due to HRI values > 1. Therefore, it can be suggested that groundwater found in above stated areas is unsafe for drinking purposes and must be treated before consumption.","PeriodicalId":19846,"journal":{"name":"Pakistan Journal of Analytical & Environmental Chemistry","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44637442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Muhammad Shahzeb Khan, M. Asif, Amina Khatoon, Shafia Arshad, Shagufta Usman, Iqra Karim
{"title":"Molecularly Imprinted Polymers: Promising Tool for the Human Virus Detection","authors":"Muhammad Shahzeb Khan, M. Asif, Amina Khatoon, Shafia Arshad, Shagufta Usman, Iqra Karim","doi":"10.21743/pjaec/2021.12.01","DOIUrl":"https://doi.org/10.21743/pjaec/2021.12.01","url":null,"abstract":"Molecular imprinting is an attractive research area for synthesizing unique functional polymers with high selectivity due to template oriented active sites. Molecularly imprinted polymers (MIPs) have a wide range of applications in chemical and biological sensing, drug delivery, and solidphase extraction owing to mechanical stability, reversibility, reproducibility, and cross-validity. MIPs are compatible with natural antibodies and are being used as antibody mimics/receptors in the biomedical field. Today, viral detection is the most popular research area due to emerging viral diseases with genetic variability and drug resistance. Therefore, there is a need to control viral infections by discriminative recognition of the viral pathogens. This review summarizes the literature on the detection of human viruses by using MIPs.","PeriodicalId":19846,"journal":{"name":"Pakistan Journal of Analytical & Environmental Chemistry","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46363324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Adeyeye, O. Ibigbami, A. J. Adesina, A. R. Olatoye, Y. A. Gbolagade
{"title":"Evaluation of Residual Polycyclic Aromatic Hydrocarbon Concentrations of Processed and Unprocessed Fish Body Parts: a Human Health Risk Assessment","authors":"E. Adeyeye, O. Ibigbami, A. J. Adesina, A. R. Olatoye, Y. A. Gbolagade","doi":"10.21743/pjaec/2021.12.11","DOIUrl":"https://doi.org/10.21743/pjaec/2021.12.11","url":null,"abstract":"Concentrations of polycyclic aromatic hydrocarbons (PAHs) from a dry extract of fresh and smoked body parts of Clarias gariepinus were examined to determine associated potential human health risks. Gas chromatography analysis was employed for the PAHs determination. The PAHs levels ranged from 0.001 µg/kg [indeno (1,2,3-cd)pyrene] to 11.7 µg/kg (acenaphthene) in fresh (extract), while the smoked (extract) showed 0.001 µg/kg [indeno (1,2,3-cd)pyrene] to 12.7 µg/kg (pyrene). The liver and head smoked were observed to be more contaminated as compared to fresh parts. Individual PAHs in the fish parts were less than the 12.0 µg/kg limit in food as set by the European Union (EU). Although there was evidence of contamination, the potential health risk associated with the fish consumption revealed no observable potential health risk to consumers.","PeriodicalId":19846,"journal":{"name":"Pakistan Journal of Analytical & Environmental Chemistry","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46889902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Spectrofluorimetric Determination of Some N. Containing Medicines Using Rhodamine 6G as a Chromogenic Reagent","authors":"Theia’a N. Al-Sabha, Mohamed Y. Dhamra","doi":"10.21743/pjaec/2021.12.15","DOIUrl":"https://doi.org/10.21743/pjaec/2021.12.15","url":null,"abstract":"A sensitive spectrofluorimetric method has been developed for the analysis of some medicines containing primary, secondary, and tertiary amino groups, namely Diclofenac (DIC), Domperidone (DOM), Famotidine (FAM), and Propranolol (PRO), in their pure and medicinal forms. The method is based on the quenching of the fluorescence intensity of rhodamine 6G (R6G) through the formation of ion-pair complexes between the above medicines and the R-6G reagent, which is measured at 552 nm after excitation at 402 nm. The calibration graphs were rectilinear in the concentration ranges of 0.10- 9.00, 0.05-15.00, 0.10-14.0 and 0.05-5.00 µg mL-1 for above medicines respectively. The recovery (%) values were ranged between 99.45%- 100.97%. The detection limits ranged in the concentration of 0.243-0.754 µg/mL, and the limits of quantitation were 0.806- 2.420 µgmL-1 for all drugs. The method was successfully applied for the determination of these drugs in their pharmaceutical preparations.","PeriodicalId":19846,"journal":{"name":"Pakistan Journal of Analytical & Environmental Chemistry","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47002235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}